The effect of LHRH-A2 alone and in combination with pimozide on induction of final maturation in Three-spot gourami (Trichogaster tricopterus)
Subject Areas : Marine BiologyT. Naji 1 , H. Hosseinzadeh sahafi 2 , G. Shakibaian 3 , M. K. Jazebizadeh 4 , Sh. Ejtemaei Mehr 5
1 - Pharmaceutical Sciences Branch, Islamic Azad University
2 - Iranian Fisheries Research Organization
3 - Pharmaceutical Sciences Branch, Islamic Azad University
4 - Lar Consulting Engineering
5 - Medical Sciences Branch, Tehran University
Keywords: LHRH-A2, Pimozide, Three-spot gourami, Final maturation,
Abstract :
The effect of LHRH-A2 alone and combined with dopamine antagonist (pimozide) on induction of final maturation were investigated in Three-spot gourami. Therefore, 80 female fish were evaluated in 8 treatments. The fishes in different groups were treated with LHRH-A2 (2, 8 µg per fish), pimozide (50µg per fish) and the combination of LHRH-A2 (2, 8 µg per fish) and pimozide(50µg per fish), there were 3 groups as control. Controls received the mixture of DMSO/PG (1:9) and FIA/Saline (1:1). One group also was inject. 7 days after injection the mean of gonadosomatic index percentage and histological structures of ovary were compared with control groups. Statistical analyses showed that the results of second (GSI%: 4/477) and fifth (GSI%: 5/027) Treatments in comparison with control group (GSI%: 2/92) were significant (P<0.05). This result was approved by histological study. Histological results showed that fifth treatment was more effective on induction of final maturation in Three-spot gourami.
