Histological study of seed development in Tribulus terrestris L. (Zygophyllaceae)
Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms
fahimeh
mokhtari
1
(tabriz)
elham
mohajel kazemi
2
(tabriz university)
mina
kazemian
3
(tabriz university)
mahboubeh
aliasgharpour
4
(tabriz)
ali
movafeghi
5
(tabriz university)
Keywords: Endosperm, Axial placentation, Apical cell, Phytochemical studies, Fluorescent microscope,
Abstract :
The study of embryonic and endosperm development in different plant species is important from a phylogenetic and systematic point of view. The aim of the present study was to investigate the histological evaluation of seed development in Tribulus terrestris L. by microscopic analysis. Samples were studied at different developmental stages after fixation by different histological techniques. The results showed that the ovules were anatropous, bilayer and axially placentation. Endosperm was observed in the early stages, which was a nuclear type that changed to a cell type during embryonic development. In the latest stages, remnants of the endosperm were observed as a net-like structure around the cotyledons. According to histological studies, divisions occurred faster in the basal cell than in the apical cell. The suspender was completely degenerated during the torpedo embryo stage. According to cyto-chemical studies, accumulation of starch grains in the seed coat was observed in the early stages of embryonic development. Moreover in the mature embryo, storage components contained protein bodies and lipid compounds. The seed coat consists of two layers, the outer layer of which contains calcium oxalate crystals. Fluorescent microscopic analysis showed that a cumulative lipid nature of waxes was observed in the grain. It seems that the study of features such as the pattern of organ formation in this plant can be effective in understanding the heterogeneity between species of the Zygophyllaceae family.
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