The Effects of Juglans Leaf Extract on Prevention of Molecular and Tissue Damages and Enzymatic changes in Chicken Liver Induced by CCl4

Subject Areas : Developmental biology of plants and animals , development and differentiation in microorganisms

Nazila Vahidi Eyrisofla ¹ , Vida Hojati ² , Mohammad-Reza Yazdian ³ , Morteza Zendehdel ⁴ , Hooman Shajiee ⁵

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Medical Science, Qom Branch, Islamic Azad University, Qom, Iran
4 - Department of Basic sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Received: 2018-06-30 Accepted : 2018-09-22 Published : 2019-05-22

Keywords: chicken, prevention, liver, Juglans, ccl4,

Abstract :

Today, the use of additives such as antibiotics and growth hormone and etc which increasing production efficiency in breeding broiler chickens is inevitable and has side effects such as liver damage. Oxidative stress occurs due to imbalance between oxidant and antioxidants. Studies have shown juglans leaves have an antioxidant effect on free radicals. The aim of the present study is to evaluate the possible effect of juglans leaf extract on CCL4- iduced liver damages (molecular and tissue) and enzymes changes in chicken. 50 chickens were used and classified in 5. Treatment groups received 0.5, 1 and 1.5 mg/kg of juglans leaf extract from day 21 of the experiment. Healthy and poisoned groups were without any extracts receive. On the 35th day of the experiment, 1 cc of CCL4 injected intra peritoneally into the poison control and experimental groups. Blood and liver tissue sampling was done. At high doses of juglans leaf extract, the histopathology results showed that the cells and vessels were regularly curable and sinusoids were healthy. Expression of BCL2 increased and BID expression reduced. Enzymatic tests including SGOT, SGPT, ALK.PHOS, GGT showed reducing in compare with control (P<0/05). It showed that juglans leaves extract boosts the BCL2 (anti-apoptotic gene) and reduces BID (apoptosis gene) in liver of chicken (P<0/001). It prevents the liver cells from disintegrating and destroys sinusoids and liver blood vessels. The high doses of juglans leaf extract cause liver resistance to ccl4 toxicity in chicken.