تخمین برون تنی تولید نیتروژن میکروبی و واکنش‪های تخمیری شکمبه‪ای به مقادیر و طول زمان استفاده از یک مخلوط تجاری آنزیم

Subject Areas : Camel

ع.  فرامرزی-گرمرودی ¹ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
م.  دانش مسگران ² (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)
ع.ر.  وکیلی ³ (Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran)

Received: 2015-04-27 Accepted : 2015-05-31 Published : 2016-03-01

Keywords: آنزیم, تجزیه‪پذیری, متان, نیتروژن میکروبی,

Abstract :

هدف آزمایش حاضر برآورد کمی فراسنجه‪های تولید گاز، تولید متان و تولید نیتروژن میکروبی جیره کاملاً مخلوط عمل‪آوری شده با سطوح مختلف مخلوط آنزیمی ناتوزایم® به روش برون تنی بود. جیره کاملاً مخلوط حاوی سیلوی ذرت، علف خشک یونجه، کاه گندم و کنسانتره به ترتیب به میزان 390، 110، 22 و 478 گرم در کیلوگرم ماده خشک بود. دویست و پنجاه میلی‪گرم از جیره پایه آسیاب (1 میلی‪متر) و خشک شد (4=n) و درون شیشه‪های انکوباسیون (125 میلی‪لیتر) ریخته شد. محلولی از مخلوط آنزیمی 0، 12 و 24 ساعت پیش از شروع انکوباسیون (96 ساعت) به شیشه‪های حاوی جیره پایه افزوده شد به گونه‪ای که میزان آنزیم در شیشه‪ها به 84/0، 68/1 و 52/2 میلی‪گرم در گرم ماده خشک رسید. پس از اتمام انکوباسیون، نیمه عمر تولید گاز محاسبه شد. آزمایش تولید گاز دیگری بر اساس نیمه عمر تولید گاز برای اندازه‪گیری حجم گاز، تولید متان و ماده خشک باقی مانده انجام شد. سطح آنزیم تأثیر معنی‪داری بر فراسنجه‪های تولید گاز و پاسخ‪های تخمیری به جز تولید نیتروژن میکروبی نداشت. آغشته‪سازی خوراک پایه 24 ساعت پیش از شروع انکوباسیون به طور معنی‪داری (05/0>P) تجزیه‪پذیری ماده خشک و حجم گاز را کاهش داد ولی درصد و حجم متان، میلی‪لیتر متان تولید شده به ازای میلی‪گرم ماده خشک ناپدید شده و تولید نیتروژن میکروبی افزایش یافت. آغشته‪سازی خوراک پایه، 12 و 24 ساعت پیش از شروع انکوباسیون به طور معنی‪داری (05/0>P) حجم گاز تجمعی را به ترتیب 16 و 8 درصد افزایش ولی ثابت نرخ تولید گاز را به ترتیب 31 و 13 درصد کاهش دادند.

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