چند شکلی‌ در ژن گیرنده شماره یک-A ملاتونین و ارتباط آن با نرخ زایش در گوسفندان نژاد زل و نائینی

Subject Areas : Camel

ن.  مرادی ¹ (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ق.  رحیمی ² (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ن.  نظیفی ³ (Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran)
ع.  نوربخش ⁴ (Department of Animal Science, Orumie University, Orumie, Iran)

Received: 2013-01-05 Accepted : 2013-04-16 Published : 2014-03-01

Keywords: زل, چند شکلی, ژن گیرنده شماره یک-A ملاتونین, نائینی, نرخ زایش,

Abstract :

تاثیر ژن 1A گیرنده ملاتونین بر نرخ زایش در 150 میش نژاد گوسفند زل و نائینی مورد مطالعه قرار گرفت. به‌ منظور تعیین چند شکلی­ها از تکنیک  PCR-RFLPبا کمک دو آنزیم محدود کننده MnlI و RsaI استفاده شد. دو آلل M و C در هر دو نژاد دارای بیشترین فراوانی بودند. ژنوتیپ  MMدر نژاد زل با فراوانی 52/0 و در نژاد نائینی با فراوانی 60/0 فراوان‌ترین ژنوتیپ بود. در نژاد زل ژنوتیپ CT با فراوانی 45/0 و در نژاد نائینی ژنوتیپ CC با فراوانی 44/0 بیشترین فراوانی را نشان دادند. ژنوتیپ  MMCTبا فراوانی (25/0) فراوان‌ترین ژنوتیپ بود. در آنالیز تجزیه واریانس اختلاف معنی‌داری بین ژنوتیپ‌های  MTNR1Aو صفت نرخ زایش در نژاد نائینی نشان داده نشد، در حالی‌که ژنوتیپ‌های mm و Mm در نژاد زل عملکرد بهتری را در میانگین نرخ زایش نشان دادند. به‌ نظر می‌رسد با انتخاب گوسفندان با این ژنوتیپ‌ها بتوان نرخ زایش در نژاد زل را بهبود بخشید. آلل‌های mC و mT در هر دو نژاد زل و نائینی تأثیر مثبتی بر نرخ بره‌زایی نشان دادند. ژنوتیپ mmCT بیشترین میانگین نرخ زایش را در بین سایر ژنوتیپ‌ها داشت. در پایان، چند شکلی در جایگاه ملاتونین می‌تواند تنها بخشی از تنوع ژنتیکی فعالیت جنسی فصلی در این دو نژاد را توجیه نماید در نتیجه عملکرد سایر ژن‌ها را هم باید مورد توجه قرار داد.

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