Polymorphism of the Melatonin Receptor 1A Gene and Its Association with Litter Size in Zel and Naeini Sheep Breeds
محورهای موضوعی : Camelن. مرادی 1 , ق. رحیمی 2 , ن. نظیفی 3 , ع. نوربخش 4
1 - Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
4 - Department of Animal Science, Orumie University, Orumie, Iran
کلید واژه:
چکیده مقاله :
The influence of melatonin receptor 1A gene on litter size was studied in 150 ewes from Zel (n=100) and Naeini (n=50) sheep breeds. Two restriction fragment length polymorphism (RFLP) analyses were done to determine all genotypes occurring by variations at MnlI and RsaI restriction sites. The M and C were the most frequent alleles in both of these breeds. The MM genotype was predominant in Zel and Naeini breeds (0.52 and 0.60, respectively). The CT genotype (0.45) has the highest frequency in Zel and the CC genotype (0.44) was predominant in Naeini breed. The MMCT genotype was identified with the highest frequency of 0.25. There were no significant differences between melatonin receptor 1A (MTNR 1A) genotypes and litter size in Naeini ewes. But it seems that selection of animals with mm and Mm genotypes can progress mean litter size in Zel breed. The positive effect of mC and mT alleles was significant on lambing rate in Zel and Naeini breeds. However, the mmCT genotype has considerably greater mean litter size than the other ones. Finally, the MTNR 1A polymorphism can explain only a small part of the genetic variability on seasonal sexual activity between these breeds. Therefore, the implication of other genes must be noticed.
تاثیر ژن 1A گیرنده ملاتونین بر نرخ زایش در 150 میش نژاد گوسفند زل و نائینی مورد مطالعه قرار گرفت. به منظور تعیین چند شکلیها از تکنیک PCR-RFLPبا کمک دو آنزیم محدود کننده MnlI و RsaI استفاده شد. دو آلل M و C در هر دو نژاد دارای بیشترین فراوانی بودند. ژنوتیپ MMدر نژاد زل با فراوانی 52/0 و در نژاد نائینی با فراوانی 60/0 فراوانترین ژنوتیپ بود. در نژاد زل ژنوتیپ CT با فراوانی 45/0 و در نژاد نائینی ژنوتیپ CC با فراوانی 44/0 بیشترین فراوانی را نشان دادند. ژنوتیپ MMCTبا فراوانی (25/0) فراوانترین ژنوتیپ بود. در آنالیز تجزیه واریانس اختلاف معنیداری بین ژنوتیپهای MTNR1Aو صفت نرخ زایش در نژاد نائینی نشان داده نشد، در حالیکه ژنوتیپهای mm و Mm در نژاد زل عملکرد بهتری را در میانگین نرخ زایش نشان دادند. به نظر میرسد با انتخاب گوسفندان با این ژنوتیپها بتوان نرخ زایش در نژاد زل را بهبود بخشید. آللهای mC و mT در هر دو نژاد زل و نائینی تأثیر مثبتی بر نرخ برهزایی نشان دادند. ژنوتیپ mmCT بیشترین میانگین نرخ زایش را در بین سایر ژنوتیپها داشت. در پایان، چند شکلی در جایگاه ملاتونین میتواند تنها بخشی از تنوع ژنتیکی فعالیت جنسی فصلی در این دو نژاد را توجیه نماید در نتیجه عملکرد سایر ژنها را هم باید مورد توجه قرار داد.
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