Prevalence and phylogenetic analysis of Listeria monocytogenes isolated from the fillets of two farmed fish in Shahrekord in 2018

Subject Areas : Aquatic Products

Esmaeil Pirali Khairabadi ¹ , Seyedh Sedigheh Mousavi ² , Hassan Momtaz ³ , Farzaneh Nikokhah ⁴ , Seyed Pejman Hosseini Shekarabi ⁵ , Mehdi Raissy ⁶

1 - Department of Fisheries Engineering and Sciences, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Chaharmahal and Bakhtiari, Iran.
2 - Department of Fisheries Engineering and Sciences, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Chaharmahal and Bakhtiari, Iran
3 - Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4 - Department of Fisheries Engineering and Sciences, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Chaharmahal and Bakhtiari, Iran
5 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، گروه شیلات، تهران، ایران
6 - Department of Aquatic Animal Health, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Received: 2020-09-02 Accepted : 2020-11-14 Published : 2020-12-21

Keywords: Rainbow trout, Common carp, Listeria, Phylogenetics,

Abstract :

Fish and other aquatic animals, directly and indirectly, can be contaminated with Listeria monocytogenes and consequently, fish and their products are considered a carrier for this bacterium. The present study investigated the contamination of farmed rainbow trout and common carp with this bacterium as well as the phylogenetics of the isolates. A total of 50 samples of farmed fish were obtained from local fish supply centers in Shahrekord City from July to September 2018. Based on the molecular experiments, 18 (36%) of the 50 studied samples were contaminated with L. monocytogenes as 26% and 10% of the isolates were found in trout and carp, respectively. From 18 isolates, 12 samples (66.66%) belonged to serotype 4b, one belonged to serotype 1/2a and the other 5 samples belonged to serotype 1/2b. According to the results of the present study and regarding the importance of the bacteria in terms of public health, continuous and accurate hygienic monitoring seems to be necessary.