استخراج تریگونلین از پلاسما با استفاده از کامپوزیت مغناطیسی جدید از طریق به کار گیری طراحی آزمایش
محورهای موضوعی : فصلنامه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی
مهدیه عباسی میمند
1
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مریم کاظمی پور
2
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مهدی انصاری دوگاهه
3
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مهدی شهیدی زندی
4
1 - دانشجوی دکتری، گروه شیمی، دانشگاه آزاد اسلامی واحد کرمان، کرمان، ایران
2 - استاد، گروه شیمی، دانشگاه آزاد اسلامی واحد کرمان، کرمان، ایران
3 - استاد، گروه کنترل غذا و دارو، دانشکده داروسازی، دانشگاه علوم پزشکی کرمان، کرمان، ایران
4 - دانشیار، گروه شیمی، دانشگاه آزاد اسلامی واحد کرمان، کرمان، ایران
کلید واژه: استخراج فاز جامد مغناطیسی, بنتونیت, پلاسما, تریگونلین, بتاسیکلودکسترین,
چکیده مقاله :
در پژوهش حاضر ترکیب بنتونیت/نانوذرات آهن/بتاسیکلودکسترین (B/I/C) به عنوان یک جاذب موثر به منظور جداسازی و پیش‌تغلیظ تریگونلین سنتز و شناسایی شد. شرایط تجربی برای یافتن متغیرهای مهم به منظور جذب سطحی تریگونلین روی جاذب بنتونیت/ بتاسیکلودکسترین/ نانوذرات آهن شامل زمان ، مقدار جاذب وpH بود. برای عمل واجذب متغیرهایی مثل دما ، غلظت سدیم کلرید ، زمان و حجم محلول نمک مورد بررسی قرار گرفتند میکروسکوپ الکترونی پویشی (SEM)، طیف‌سنجی مادون قرمز (FT-IR)، میکروسکوپ الکترونی عبوری (TEM)، میکروسکوپ نیروی اتمی (AFM)، مغناطیس‌سنج نمونه ارتعاشی (VSM)، آنالیز سطح BET و پراش پرتوایکس ( (XRD )برای تعیین ساختار جاذب سنتز شده مورد استفاده قرار گرفتند. سپس طراحی آزمایش به منظور بهینه‌سازی فاکتورهایی که فرایند استخراج را تحت تاثیر قرار می‌دهند به کار برده شد. تریگونلین در نمونه‌های پلاسما استخراج و با روش HPLC-UV اندازه‌گیری شد. روش MSPE (استخراج فاز جامد مغناطیسی) به طور موثر قادر به استخراج تریگونلین بود. منحنی کالیبراسیون درگستره غلظتی 10-0.05 میکروگرم بر میلی‌لیتر خطی با ضریب همبستگی (0.9998)، به‌دست آمد. حد تشخیص (LOD) و حد کمی‌سازی (LOQ) روش به ترتیب 0.005، 0.018 میکروگرم بر میلی‌لیتر و میزان بازده استخراج 114 درصد به‌دست آمد. این جاذب به طور موفقیت‌آمیزی برای اندازه‌گیری تریگونلین در پلاسما به کاربرده شد.
Bentonite-β-cyclodextrin-iron nanoparticles (Ben-βCD-INP) composite was synthesized,characterized, and utilized like an innovative magnetic solid-phase extraction ( MSPE) adsorbent to separate and preconcentrate trigonelline . Experiment conditions to find important variables of trigonelline adsorption on the Ben-βCD-INP were A: time, B: Adsorbent amount, C: pH and for desorption were A: temperature, B: concentration, C: time, and D: Volume .. The adsorbent contributes significantly in the extraction process. The INP in the nano composite makes it easy to remove the adsorbent with the help of a magnet. Scanning electron microscopy( SEM ), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), and X-ray diffraction (XRD) were used to characterize the synthesized adsorbent. Experimental design was used to optimize the factors affecting extraction. trigonelline in plasma samples was extracted and evaluated by a reversed-phase HPLC–UV method. The findings have shown that MSPE was able to extract trigonelline efficiently. The developed HPLC–UV procedure for the determination of trigonelline in human plasma, showed linear relation shipin the concentration interval of 0.05-10 mg/L (r = 0. 9998) with the limit of detection (LOD), the limit of quantitation (LOQ) and recovery (%) of 0.005, 0.018 mg/L, and 114%, respectively. The procedure was successfully used to determine the concentration of trigonelline in human plasma. This study offers a promising hybrid nano bio material adsorbent in biomedical nanotechnology to selective measurement of the drug or supplements such as trigonelline from plasma.
1- Mohamadi N, Sharififar F, Pournamdari M, Ansari M. A review on biosynthesis, analytical techniques, and pharmacological activities of trigonelline as a plant alkaloid. Journal of dietary supplements. 2018; 15(2):207-22.
2- Hosseini Z, Hassanloo T, Kowsari M, Majidian M. Trigonelline as an anti-diab-etic metabolite increased in inoculated fen-ugreek by Trichoderma. Advanced Resea-rch in Microbial Metabolites and Techno-logy. 2019;2(1):65-75.
3- Lorigooini Z, Dehsahraei KS, Bijad E, Dehkordi SH, Amini-Khoei H. Trigone-lline through the attenuation of oxidative stress exerts antidepressant-and anxiolytic-like effects in a mouse model of maternal separation stress. Pharmacology. 2020;105 (5-6):289-99.
4- Laila O, Murtaza I, Abdin MZ, Ahmad S, Khan MS. Development and validation of a high-performance thin-layer chromate-graphy based method for the quantification of trigonelline in fenugreek (Trigonella fo-enum-graecum) seeds. JPC-Journal of Pla-nar Chromatography-Modern TLC. 2019; 32(2):95-102.
5- Perrone D, Donangelo CM, Farah A. Fast simultaneous analysis of caffeine, tri-gonelline, nicotinic acid and sucrose in coffee by liquid chromatography-mass spe-ctrometry. Food chemistry. 2008;110(4): 1030-5.
6- Cheng Z-X, Jin-Jun W, Zhong-Qiu L, Na L. Development of a hydrophilic inte-raction chromatography-UPLC assay to det ermine trigonelline in rat plasma and its application in a pharmacokinetic study. Chinese journal of natural medicines. 2013;11(2):164-70.
7- Zhang J, Liu D, Meng X, Shi Y, Wang R, Xiao D, et al. Solid phase extraction based on porous magnetic graphene oxi-de/β-cyclodextrine composite coupled with high performance liquid chromatography for determination of antiepileptic drugs in plasma samples. Journal of Chromatogra-phy A. 2017;1524:49-56.
8- Midttun Ø, Ulvik A, Nygård O, Ueland PM. Performance of plasma trigonelline as a marker of coffee consumption in an epidemiologic setting. The American jour-nal of clinical nutrition. 2018;107(6):941-7.
9- Wen C, Lin C, Cai X, Ma J, Wang X. Determination of sec-O-glucosylhamaudol in rat plasma by gradient elution liquid chromatography-mass spectrometry. Jour-nal of Chromatography B. 2014;944:35-8.
10- Shi L-n, Lin Y-M, Zhang X, Chen Z-l. Synthesis, characterization and kinetics of bentonite supported nZVI for the removal of Cr (VI) from aqueous solution. Che-mical Engineering Journal. 2011;171(2): 612-7.
11- Khan, K.A., Suidan, M.T. and Cross, W.H., “Anaerobic activated carbon filter for the treatment of phenol-bearing waste-water.” J. Water Pollut. Control Fed., 1519-1532, 1981.
12- Faraji, M., Yamini, Y., Rezaee, M., "Magnetic nanoparticles: synthesis, stabili-zation, functionalization, characterization, and applications", J. Iran. Chem. Soc., 7, 1-37, 2010.
13- Márquez-Sillero I, Cárdenas S, Valcár-cel M. Determination of water-soluble vit-amins in infant milk and dietary supple-ment using a liquid chromatography on-line coupled to a corona-charged aerosol detector. Journal of Chromatography A. 2013;1313:253-8.
14- Shahwan T, Üzüm Ç, Eroğlu AE, Lieberwirth I. Synthesis and characteri-zation of bentonite/iron nanoparticles and their application as adsorbent of cobalt ions. Applied Clay Science. 2010;47(3-4):257-62.
15- Abdolmohammad-Zadeh H, Talleb Z. Magnetic solid phase extraction of gemfi-brozil from human serum and pharmacy-eutical wastewater samples utilizing a β-cyclodextrin grafted graphene oxide-magn-etite nano-hybrid. Talanta. 2015;134:387-93.
16- Mohamadi N, Sharififar F, Pournam-dari M, Ansari M. Determination of trig-onelline in human plasma by magnetic solid-phase extraction: a pharmacokinetic study. Nanomedicine. 2020;16(4):323-33.
17- Hashemian M, Ghasemi-Kasman M, Ghasemi S, Akbari A, Moalem-Banhangi M, Zare L, et al. Fabrication and evalu-ation of novel quercetin-conjugated Fe3O4-β-cyclodextrin nanoparticles for potential use in epilepsy disorder. International journal of nanomedicine. 2019;14:6481.
18- Khoobi M, Khalilvand‐Sedagheh M, Ramazani A, Asadgol Z, Forootanfar H, Faramarzi MA. Synthesis of polyethy-leneimine (PEI) and β‐cyclodextrin grafted PEI nanocomposites with magnetic cores for lipase immobilization and esterific-ation. Journal of Chemical Technology & Biotechnology. 2016;91(2):375-84.
19- Lang R, Yagar EF, Eggers R, Hofmann T. Quantitative investigation of trigonell-ine, nicotinic acid, and nicotinamide in foods, urine, and plasma by means of LC-MS/MS and stable isotope dilution anal-ysis. Journal of agricultural and food che-mistry. 2008;56(23):11114-21.
20- Cheng Z-X, Jin-Jun W, Zhong-Qiu L, Na L. Development of a hydrophilic inter-action chromatography-UPLC assay to det-ermine trigonelline in rat plasma and its application in a pharmacokinetic study. Chinese journal of natural medicines. 2013;11(2):164-70.
21- Pang H-Q, Tang Y-P, Cao Y-J, Tan Y-J, Jin Y, Shi X-Q, et al. Comparatively evaluating the pharmacokinetic of fifteen constituents in normal and blood defici-ency rats after oral administration of Xin-Sheng-Hua Granule by UPLC–MS/MS. Journal of Chromatography B. 2017;1061: 372-81.
22- Caporaso N, Whitworth MB, Grebby S, Fisk ID. Non-destructive analysis of sucrose, caffeine and trigonelline on single green coffee beans by hyperspectral ima-ging. Food Research International. 2018; 106:193-203.
23- Abd Malek NN, Jawad AH, Abdulha-meed AS, Ismail K, Hameed B. New magnetic Schiff's base-chitosan-glyoxal/fly ash/Fe3O4 biocomposite for the removal of anionic azo dye: An optimized process. International journal of biological macrom-olecules. 2020;146:530-9.
24- Kudr J, Haddad Y, Richtera L, Heger Z, Cernak M, Adam V, et al. Magnetic nanoparticles: From design and synthesis to real world applications. Nanomaterials. 2017;7(9):243.
25- Huang X, Yi C, Fan Y, Zhang Y, Zhao L, Liang Z, et al. Magnetic Fe3O4 nan-oparticles grafted with single-chain antib-ody (scFv) and docetaxel loaded β-cycl-odextrin potential for ovarian cancer dual-targeting therapy. Materials Science and Engineering: C. 2014;42:325-32.
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