تغییرات آنزیم‌های مرتبط با دفاع در گیاهچه چغندرقند رقم Brigita علیه ویروس پیچیدگی شدید بوته چغندرقند (BSCTV) وباکتری Pseudomonas fluorescens استرین CHA0
محورهای موضوعی : گیاه پزشکیعفت عالم‌زاده 1 , کرامت‌اله ایزدپناه 2 , موسی زارعی 3 , علی‌اکبر بهجت‌نیا 4
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کلید واژه: Antioxidant enzymes, BSCTV, Pseudomonas fluorescens strain CAH0, ویروس پیچیدگی شدید بوته چغندرقند, آنزیم‌های ضداکسنده, باکتری Pseudomonas fluorescens استرین CHA0,
چکیده مقاله :
تنش‌های مختلف محیطی شامل تنش‌های زنده و غیرزنده سبب تولید رادیکال‌های آزاد اکسیژن می‌شوند از جمله این تنش‌ها می‌توان به برهمکنش گیاه-ویروس اشاره کرد. گیاهان با دارا بودن سیستم ضد اکسنده که شامل ترکیبات آنزیمی و غیر آنزیمی می‌باشد معمولا سطوح رادیکال‌های آزاد اکسیژن را در سلول در حد متعادل نگه می‌دارند. هدف از انجام این تحقیق، بررسی اثر ویروس پیچیدگی شدید بوته چغندرقند (BSCTV) و همچنین اثر این ویروس به همراه باکتری Pseudomonas fluorescens استرین CHA0 بر نحوه فعالیت آنزیم‌های ضداکسنده در یک رقم حساس چغندرقند (Brigita) می‌باشد فعالیت ترکیبات آنزیمی ضداکسنده در این بررسی با استفاده از دستگاه طیف سنج در دمای اتاق اندازه‌گیری شد. نتایج حاصل از بررسی نشان داد که وقتی علائم شدید آلودگی روی برگ‌ها گسترش می‌یاید و مقدار ویروس به حداکثر می‌رسد افزایش معنی‌داری در میزان فعالیت آنزیم‌های ضداکسنده آسکوربیت پراکسیداز، پراکسیداز و کاهش معنی‌داری در میزان فعالیت آنزیم کاتالاز مشاهده می‌شود. همچنین در تنش ویروس به همراه باکتری میزان فعالیت آنزیم‌ها در همه موارد با مقداری کاهش نسبت به گیاهان تحت تنش ویروس به تنهایی همراه بوده‌‌است.
Various environmental stresses including biotic and abiotic stresses lead to the production of reactive oxygen species (ROS). Accumulation of reactive oxygen species often have been detected in plant pathogen- interactions. Plants with a combination of enzymatic and non-enzymatic antioxidant systems usually ROS levels in cells kept in moderation. The aim of the present work is to study the effect of BSCTV infection and Pseudomonas fluorescens strain CAH0 on the activities of antioxidant enzymes in sugar beet plants by using a UV / Vis 2100 spectrophotometer. The results of the study showed when severe symptoms of the infection developed on the leaves and the virus content reached its maximum, a significant increase in the activities of several antioxidant enzymes, ascorbate peroxidase and peroxidase and a significant decrease in the developed on the leaves and the virus content reached its maximum, a significant increase in the activity of antioxidant enzyme, Catalse, was found. Also in virus's tension associated with bacteria, enzyme activity was reduced in all items.
منابع
Al-Aghabary, K., Zhu, Z. & Shi, Q.H., 2004. Influence of silicon supply on chlorophyll content, chlorophyll fluorescence, and antioxidative enzyme activities in tomato plants under salt stress. Journal of Plant Nutrition, 27: 2101-2115.
Blokhina, O., Virolainen, E. & Fagerstedt, K.V. 2003. Antioxidants, Oxidative Damage and Oxygen Deprivation Stress. Annals of Botany, 91: 179–194.
Bolok Yazdi, H.R., Heydarnejad, J. & Massumi, H. 2008. Genome characterization and genetic diversity of Beet curly top Iran virus: a geminivirus with a novel nonanucleotide. Virus Genes, 36: 539-545.
Briddon, R., Stenger, D., Bedford, I., Stanley, J., Izadpanah, K. & Markham, P. 1998. Comparison of a Beet curly top virus isolate originating from the old world with those from the new world. European Journal of Plant Pathology, 104: 77-84.
Chance, B. & Maehly, A.C. 1995. Assay of catalases and peroxidases, pp. 764-775, In: Colowick S.P. & Kaplan N.O. (Ed.) Methods in enzymology. Acad. Press, New York.
Clarke, S.F., Guy, P.L., Burritt, D.J. & Jameson, P.E. 2002. Changes in the activities of antioxidant enzymes in response to virus infection and hormone treatment. Physiologia Plantarum, 114: 157-164.
Ebadzad Sahraei, G., Behjatnia, S.A.A. & Izadpanah, K. 2008. Viruses causing curly top of beet and other plants in Fars. 18th Iranian plant protection congress, 25-28 Agu. 2008, Hamedan, Iran.
Fattahi, Z., Behjatnia, A., Afsharifar, A., Hamzeh Zarghani, H. & Izadpanah, K. 2012. Screening of sugar beet cultivars for resistance to Iranian isolate of Beet severe curly top virus using an infectious clone of the virus. Iranian Journal of Plant Pathology, 48: 111-112. (In Persian with English abstract)
Garmendia, I., Goicoechea, N. & Aguireolea, J. 2004. Effectiveness of three Glomus species in protecting pepper (Capsicum annuum L.) against Verticillium wilt. Biological Control, 31: 296-305.
Hakmaoui, A., Perez-Bueno, M. L., Garcin-fontana, B., Camejo, D., Jimenez, A.,Sevilla, F. & Baron., M., 2012. Analysis of the response of Nicotiana benthamiana to infection with two strains of pepper mild mottle virus. Journal of Experimental Biology, 63:5487-96.
Hernández, J.A., Diaz-Vivancos, P., Rubio, M., Olmos, E., Ros-Barceló, A. & Martínez-Gómez, P. 2006. Long-term PPV infection produces an oxidative stress in a susceptible apricot cultivar but not in a resistant cultivar. Physiologia Plantarum, 126, 140–152.
Hernández, J.A., Gullner, G., Clemente-Moreno, M.J., Künstler, A., Juhász, C., Díaz-Vivancos, P. & Király, L. 2015. Oxidative stress and antioxidative responses in plant-virus interactions. Physiological and Molecular Plant Pathology, In Press.
Kohler, J., Hernandes, J.A., Caravaca, F. & Roldan, A. 2009. Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress. Enviromental and Experimental Botany, 65: 245-252.
Maurhofer, M., Hass, D., Meuwly, P., Metraux, J-P. & Defago, G. 1994. Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHA0: influence of the gacA gene and of pyoverdine production. Phytopathology, 84: 139-146.
Maurhofer, M., Keel, C., Schnider, U., Voisard, C., Hass, D. & Defago, G. 1992. Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHAO on its disease suppressive capacity. Phytopathology, 82: 190-195.
Milosevic, S., Simonovic, A., Cingel, A., Jevremovic, S., Todorovic, S., Filipovic, B. & Suboti, A. 2012. Response of antioxidative enzymes to long-termTomato spotted wilt virus infection and virus elimination by meristem-tip culture in two Impatiens species. Physiological and Molecular Plant Pathology, 79: 79-88.
Nakano, A. & Asada, K. 1987. Purification of ascorbate peroxidase in spinach chloroplasts: Its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant and Cell Physiology, 28: 131–140.
Polle, A., Otter, T. & Seifert, F. 1994. Apoplastic peroxidases and lignification in needles of Norway spruce (Picea abies L.). Plant Physiology, 106: 53–60.
Riedle-bauer, M. 2000. Role of Reactive Oxygen Species and Antioxidant Enzymes in Systemic Virus Infections of Plants. Journal of Phytopathology, 148: 297-302.
Sgherri, C, Ranieri, A. & Quartacci, M.F. 2013. Antioxidative responses in Vitis vinifera infected by grapevine fanleaf virus. Journal of Plant Physiology, 170: 121– 128.
Song, X.S., Wang, Y.J., Mao, W.H., Shi, K., Zhou, Y.H., Nogués, S. & Yu, J.Q. 2009. Effects of cucumber mosaic virus infection on electron transport and antioxidant system in chloroplasts and mitochondria of cucumber and tomato leaves. physiologia plantarum, 135: 246-57.
Torres, M.A., Jones, J.D.G. & Dangl, J.L. 2006. Reactive oxygen species signaling in response to pathogens. Plant Physiology, 141: 373–378.
Van-Loon, L., Baker, P.A. & Pieterse, C.M. 1998. Systemic resistance induced by rhizospher bacteria. Annul Review Phytopathology, 36: 453-483.
Yi, S., Yu, S. & Choi, D. 2003. Involvement of hydrogen peroxide in repression of catalase in TMV-infected resistant tobacco. Molecules and Cells, 15: 364−369.