تاثیر فلز کادمیوم بر میزان آنزیم کاتالاز و بیومارکر دی تیروزین در برخی گونه های زراعی
محورهای موضوعی : یافته های نوین کشاورزیسیده فاطمه موسوی 1 , داود حبیبی 2 , افشین مظفری 3 , نورعلی ساجدی 4
1 - دانشگاه آزاد اسلامی، واحد اراک، ایران
2 - دانشگاه آزاد اسلامی، واحد کرج، گروه زراعت و اصلاح نباتات، کرج، ایران
3 - دانشگاه آزاد اسلامی، واحد ایلام، گروه زراعت و اصلاح نباتات، ایلام، ایران
4 - دانشگاه آزاد اسلامی، واحد اراک، گروه زراعت و اصلاح نباتات، اراک، ایران
کلید واژه: آنزیم آنتی اکسیدانت, بیومارکر بیوشیمیایی, کاتالاز و دی تیروزین,
چکیده مقاله :
این آزمایش در گلخانه تحقیقاتی دانشکده کشاورزی دانشگاه آزاد اسلامی واحد کرج در سال 89- 1388به اجرا در آمد. آزمایش بصورت فاکتوریل در قالب طرح پایه بلوک های کامل تصادفی با 3 تکرار اجرا شد. فاکتورهای آزمایش شامل میزان کادمیوم در خاک در سه سطح (صفر، 40 و 80 میلی گرم در کیلو گرم خاک خشک) و گونه گیاه زراعی در سه سطح (یونجه یکساله (Medicago rigidula)، ماشک گل خوشه ای (Vicia villosa) وکلزا(Barassica napus)) بود. نتایج آزمایش نشان داد میزان کادمیوم در خاک و گونه های زراعی بر کلیه صفات مورد مطالعه اثر معنی داری داشت. بالاترین میزان بیومارکر دی تیروزین (D-T) در کلزا (با 99/10 میکرومول بر گرم وزن تازه بافت) و پایین ترین میزان در گیاه ماشک گل خوشه ای (با 86/8 میکرومول بر گرم وزن تازه بافت) بود. بیشترین و کمترین میزان آنزیم آنتی اکسیدانت کاتالاز (CAT) به ترتیب متعلق بود یونجه (با 79/55 واحد بر میلی گرم پروتئین) و ماشک گل خوشه ای (با 77/52 واحد بر میلی گرم پروتئین). نتایج آزمایش نشان داد با افزایش غلظت کادمیوم (Cd)در خاک، مقدار بیومارکر دی تیروزین افزایش یافت اما برعکس میزان آنزیم کاتالاز کاهش یافت. دوز 80 و صفر (میلی گرم کادمیوم در کیلوگرم خاک خشک) به ترتیب با 72/11 و 52/6 (میکرومول بر گرم وزن تازه بافت) بالاترین و پایین ترین میزان بیومارکر دی تیروزین را به خود اختصاص دادند. دوز صفر و 80 (میلی گرم کادمیوم در کیلوگرم خاک خشک) به ترتیب با 30/63 و 39/49 (میکرومول بر گرم وزن تازه بافت) بالاترین و پایین ترین میزان آنزیم کاتالاز را به خود اختصاص دادند.
In order to study Effect of Cadmium on Ditirozin (D-T) Biochemical biomarker and Catalas (CAT) Antioxidant Enzyme in some Crop species the some Crop species, an experiment was conducted in experimental Greenhouse of Islamic Azad University of Karaj branch in 2009-2010 year. The studies were carried out in a Factorial experiment based on a randomized complete block design with three replactions. The factors were Cd doses into soil at three levels 0, 40 and 80 (mg Cd/kg.dw soil) and Crop species at three levels, annual Alfalfa (Medicago rigidula), Hairy vetch (Vicia villosa ) and Canola (Barassica napus). Results showed that different Crop species and Cd doses into soil had highly significant effect on all of Experiment characteristics. Highest amount of Ditirozin (D-T) Biochemical Biomarker related to Canola (with 10.99 µMol/g.Fw) and lowest of this trait related to Hairy vetch (with 8.86 µMol/g.Fw). Highest amount of Catalase (CAT) Antioxidant Enzyme related to Alfalfa (with 55.79 U/mg.protein) and lowest of this trait related to Hairy vetch(with 52.77 U/mg.protein). With increase Cd Doses into soil, Ditirozin (D-T) Biomarker content was increased, but Catalas Antioxidant Enzyme was decreased. 80 (mg Cd/kg.dw Soil) doses with 11.72 (µMol/g.Fw) and 0 (mg Cd/kg.dw Soil) doses with 6.52 (µMol/g.Fw) were highest and lowest Ditirozin (D-T) Content, respectively. 0 and 80 (mg Cd/kg.dw Soil) doses with 63.30 (U/mg.protein) and 49.39 (U/mg.protein) were highest and lowest Catalas (CAT) Antioxidant Enzyme Content, respectively.
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