The Effect of Plant Growth Regulators on Callus Induction and Regeneration in Three Chrysanthemum (Chrysanthemum grandiflorum Ramat) Cultivars Under In Vitro Culture Condition
محورهای موضوعی : مجله گیاهان زینتیMohammad Reza Shafiei 1 , Abdollah Hatamzadeh 2 , Pejman Azadi 3 , Habibollah Samizadeh Lahiji 4
1 - Ornamental Plants Research Center (OPRC), Horticultural Sciences Research Institute (HSRI), Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran.
2 - Department of Horticultural Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 - Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran
4 - Department of Agronomy Plant Breeding, Faculty of Agriculture Science, University of Guilan
کلید واژه: tissue culture, auxin, Micropropagation, Benzyl Adenine, explant,
چکیده مقاله :
Chrysanthemums are known as one of the top three flowers in the floriculture industry. This ornamental plant is traditionally propagated by cuttings. The use of in vitro subculture is expanding for plants like chrysanthemums that are propagated by asexual methods. This research evaluated the effect of plant growth regulators on callus induction and regeneration in three chrysanthemum cultivars including ‘Bonfire Yellow’, ‘Rambla’, and ‘Bella Rosa’. For callus generation, the leaf explants were cultivated in the Murashige and Skoog (MS) culture medium modified with with benzyl adenine (1, 2, or 3 mg/L BA) and naphthaleneacetic acid (0.5 or 1 mg/L NAA) in a factorial experiment based on a completely randomized design. The highest rate of regeneration of ‘Bonfire Yellow’(31.25%), ‘Rambla’(25%), and ‘Bella Rosa’ (31.25%) were obtained from the culture media containing 2 mg/L BA + 1 mg/L NAA, 1 mg/L BA + 1 mg/L NAA, and 3 mg/L BA + 0.5 mg/L NAA, respectively.
گل داوودی به‌عنوان یکی از سه گل مهم در صنعت گلکاری شناخته می‌شود. این گل به‌صورت سنتی توسط قلمه تکثیر می‌شود. استفاده از ریزازدیای درون شیشهای در گیاهانی مانند داوودی که با روش غیر جنسی تکثیر می‌شوند رو به گسترش است. این پژوهش جهت بررسی اثر تنظیم کننده‌های رشد گیاهی بر تولید کالوس و میزان باززایی در سه رقم داوودی Bonfire yellow، Rambla و Bella rosa انجام گرفت. ریزنمونه‌های برگی داوودی جهت تولید کالوس و بصورت آزمایش فاکتوریل در قالب طرح کامل تصادفی در محیط کشت موراشیگ و اسکوگ (MS) حاوی 3 درصد ساکارز و 7 گرم در لیتر آگار به همراه مقادیر مختلف تنظیم کننده‌های رشد بنزیل آدنین (1، 2 و 3 میلی گرم در لیتر) و نفتالین استیک اسید (0/5 و 1 میلی گرم در لیتر) کشت شدند. نتایج به دست آمده در سطح 1 درصد آزمون LSD معنیدار بود. محیط کشت حاوی 2 میلیگرم در لیتر BA و 1 میلیگرم در لیتر NAA در رقم Bonfire yellow، محیط کشت حاوی 1 میلیگرم در لیتر BA و 1 میلیگرم در لیتر NAA در مورد رقم Rambla و محیط کشت حاوی 3 میلیگرم در لیتر BA و 0/5 میلیگرم در لیتر NAA در رقم Bella rosa بیشترین باززایی را نشان داد.
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