Application of Nanoanalysis: Amine-Functionalized Mesoporous Silica nanoparticles for Mercury Speciation in Human Samples
Subject Areas : Journal of NanoanalysisSara Davari 1 , Hamid Shirkhanloo 2 , Negar Motakef Kazemi 3
1 - Department of Medical Nanotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences
Branch, Islamic Azad University, (IAUPS), Tehran, Iran
2 - Research Institute of Petroleum Industry (RIPI), West Entrance Blvd., Olympic Village, Tehran, Iran
3 - Department of Medical Nanotechnology, Faculty of Advanced Sciences & Technology, Pharmaceutical Sciences
Branch, Islamic Azad University, (IAUPS), Tehran, Iran
Keywords: Human blood, Amine-functionalized mesoporous silica nanoparticles, Dispersive-ionic liquid-micro-solid phase extraction, Mercury species,
Abstract :
Mercury (Hg) causes hazardous cumulative effects in humans; like central nervous system disorders,hypertension and chromosomal aberrations. Therefore, due to high toxicity and bioaccumulationfactor, mercury determination and speciation in human blood is very important. A sensitive,accurate, precise and inexpensive method was demonstrated for preconcentration and speciationof ultra-trace mercury in human blood samples. The amine-functionalized mesoporous silicananoparticles (NH2-MSNPs) as solid phase sorbent was prepared and investigated in a new andsimple dispersive-ionic liquid-micro-solid phase extraction (D-IL-μ-SPE) procedure for rapidspeciation and determination of trace inorganic and organic mercury [Hg+2 , (CH3Hg)+ and (C2H5Hg)+]in human blood samples. Under the optimal conditions, the linear range, limit of detection andpreconcentration factor were obtained 0.05-9.8 μg L−1, 15 ng L−1 and 9.6 for human blood samples,respectively (Peak Area, RSD<5%). The reusability and batch adsorption capacity of the MSNPsand NH2-MSNPs were also obtained 25 and 159 mg g-1, respectively. The developed method wassuccessfully applied for validation of methodology by standard reference materials (SRM).