Evaluation of Alginate/Collagen Edible Coatings with Betanin and Cumin to Improve the Shelf Life of Lighvan Cheese

Subject Areas : food microbiology

F. Ahmadimaram ¹ , T. Mostaghim ² , Sh. Shahriari ³

1 - M.Sc. Graduated of the Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor of the Department of Food Science and Technology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor of the Department of Chemical Engineering, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran.

Received: 2021-06-03 Accepted : 2021-06-03 Published : 2021-07-01

Keywords: Alginate, Betanin, Cumin, Cheese, Collagen,

Abstract :

Lighvan cheese is one of the most popular and consumed cheese in Iran. The aim of this study was to use coated alginates-collagen containing essential oils of cumin and betanin to improve the quality and shelf life of the Lighvan cheese. Initially, film samples containing different levels of cumin essential oils (4, 6, and 8%) and betanin (4 and 6%) were prepared, and then the physicochemical and antioxidant properties of all films were investigated. The physicochemical, antioxidant, and sensory properties of the samples of the Lighvan cheese were evaluated during 21day storage period. The results of experiments on edible films showed that the combination of essential oils of cumin and betanin into edible films increased the thickness, moisture content, water-solubility, and permeability to steam (p <0.05). The results indicated that the coated cheese samples containing cumin and betanin have effective antioxidant activity. Sensory evaluation results showed that the coated cheese samples had no negative effect on organoleptic acceptance (p <0.05). This study indicated that the addition of cumin essential oil and betanin to the alginate-collagen film had a positive effect on increasing the shelf-life of the Lighvan cheese. The selected sample contained essential oils of cumin and betanin at the concentrations of 8 and 6% respectively.

References:

Abbdellaoui, M., Bouhlali, E. & El Rhaffari, L. (2019). Chemical Composition and Antioxidant Activities of the Essential Oils of Cumin (Cuminum cyminum) Conducted Under Organic Production Conditions. Journal of essential oil-bearing plants, doi:org/10.1016/j.ijbiomac.2019.11.004.

Adams, B., Sivarooban, T., Hettiarachchy, N. S. & Johnson, M. G. (2005). Inhibitory activity against Listeria monocytogenes by soy protein edible film containing grape seed extract, nisin, and malic acid. The Student Journal of the Dale Bumpers College of Agricultural. Food and Life Sciences, 6, 3-9.

Aguirre, B. & León, R.E. (2013). Antimicrobial, mechanical and barrier properties of triticale protein films incorporated with oregano essential oil. Food Bioscience, 1, 2-9.

Ani, V., Varadaraj, M. C. & Akhilender Naidu, K. (2006). Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin. European Food Research Technology, 224, 109-115.

Arashisar, Ş., Hisar, O., Kaya, M. & Yanik, T. (2004). Effects of modified atmosphere and vacuum packaging on microbiological and chemical properties of rainbow trout (Oncorynchus mykiss) fillets. International Journal of Food Microbiology, 97, 209-214.

ASTM. (1995). Standard test methods for water vapor transmission of material E96-95. Philadelphia, PA: American Society for Testing and Material.

Atarés, L., De Jesús, C., Talens, P. & Chiralt, A. (2010). Characterization of SPI-based edible films incorporated with cinnamon or ginger essential oils. Journal of Food Engineering, 99, 384-391.

Attar, M. A., Yavarmanesh, M., Mortazavi, A., Edalatian Dovom, M. R. & Habibi Najafi, M. B. (2017). Antibacterial effects of Lactococcus lactis isolated from Lighvan cheese regarding the recognition of Nisin, Lacticin, and Lactococcin structural genes. LWT - Food Science and Technology, doi: 10.1016/j.lwt.2017.10.044.

August, A. D., Kong, H. J. & Mooney, D. J. (2006). Alginate hydrogels as biomaterials. Macromolecule Bioscience, 7, 623-633.

Brand-Williams, W., Cuvelier, M. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT- Food Science and Technology, 28, 25-30.

Cerqueira, M. A., Lima, A. M., Souza, B. W. S., Teixeira, J. A., Moreira, R. A. & Vicente, A. A. (2009). Functional polysaccharides as edible coatings for cheese. Journal of Agriculture and Food Chemistry, 57, 1456-1462.

Chen, Q., Gan, Z., Zhao, J., Wang, Y., Zhang, S., Li, J. & Ni, Y. (2014). In vitro comparison of antioxidant capacity of cumin (Cuminum cyminum L.) oils and their main components. LWT - Food Science and Technology, 55, 632-637.

Costaa, M. J., Maciela, L.C., Teixeira, J. A., Vicente, A. A. & Cerqueira, M. A. (2018). Use of edible films and coatings in cheese preservation: Opportunities and challenges. Food Research International, 107, 84-92.

Dehghani, S., Hosseini, S. V. & Regenstein, J. M. (2018). Edible films and coatings in seafood preservation: a review. Food Chemistry, 240, 505-513.

Dimitrios, B. (2006). Sources of natural phenolic antioxidants. Trends in Food Science and Technology, 17, 505-512.

Du, W. X., Olsen, C. W., Avena-Bustillos, R. J., Mchugh, T. H., Levin, C. E. & Mandrell, R. (2009). Antibacterial effects of allspice, garlic, and oregano essential oils in tomato films determined by overlay and vapor-phase methods. Journal of Food Science, 74, M390-M397.

Ebrahimi, T. & Shahriari, S. (2016). Extraction of Betanin Using Aqueous Two-Phase Systems. Bulletin of the Chemical Society of Japan, 565-572.

Emamifar, A. & Bavaisi, S. (2020). Nanocomposite coating based on sodium alginate and nano-ZnO for extending the storage life of fresh strawberries (Fragaria × ananassa Duch). Journal of Food Measurement and Characterization, doi:org/10.1007/s11694-019-00350-x

Esatbeyoglu, T., Wagner, A.E., Motafakkerazad, R. & Nakajima, Y. (2014). Free radical scavenging and antioxidant activity of betanin: Electron spin resonance spectroscopy studies and studies in cultured cells. Food Chemistry and Toxicology, doi: 10.1016/j.fct.2014.1008.1007.

Escribano, J., Pedrenp, M.A., Garcia-Carmona, F. & Munoz, R. (1998). Characterization of the antiradical activity of betalains from Beta vulgaris L. roots. Phytochemistry Analysis, 9, 124-127.

Fajardo, P., Martins, J. T., Fucinos, C., Pastrana, L., Teixeira, J. A. & Vicente, A. A. (2010). Evaluation of a chitosan-based edible film as carrier of natamycin to improve the storability of Saloio cheese. Journal of Food Engineering, 101, 349-356.

Guerra-Martínez, J., Montejano, J. & Martín-del-Campo, S. (2012). Evaluation of proteolytic and physicochemical changes during storage of fresh Panela cheese from Queretaro, Mexico and its impact in texture. CyTA - Journal of Food, 10 (4), 296-305.

Haddar, A., Sellimi, S., Ghannouchi, R., Alvarez, O.M., Nasri, M. & Bougatef, A. (2012). Functional, antioxidant and film-forming properties of tuna-skin gelatin with a brown algae extract. International Journal of Biological Macromolecules, 51(4), 477-483.

Hajlaoui, H., Mighri, H., Noumi, E., Snoussi, M., Trabelsi, N. & Ksouri, R. (2010). Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: A high effectiveness against Vibrio spp. strains. Food Chemistry and Toxicology, 48, 2186-2192.

Han, J. H. & Krochta, J. M. (1999). Water vapor permeability and wetting properties of whey protein coating on paper. American Society of Agricultural and Biological Engineers, 42, 1375-1382.

Hernandez, E. (1994). Edible coatings for lipids and resins. In J. M. Krochta, E. A. Baldwin, & M. O. Nisperos-Carriedo (Eds.), Edible coatings and films to improve food quality (pp. 279-304). Lancaster, PA: Technomic Publishing Co.

Jeon, B. H., Dempsey, B. A., Royer, R. A. & Burgos, W. D. (2003). Kinetics and mechanisms for reactions of Fe(II) with iron(III) oxides. Environment Science and Technology, 37, 3309-3315.

Jirovetz, L., Buchbauer, G., Stoyanova, A. S., Georgiev, E. V. & Damianova, S.T. (2005). Composition, quality control, and antimicrobial activity of the essential oil of cumin (Cuminum cyminum L.) seeds from Bulgaria that had been stored up to 36 years. International Journal of Food Science and Technology, 40, 305-310.

Kampf, N. & Nussinovitch, A. (2000). Hydrocolloid coating of cheeses. Food Hydrocolloids, 14 (6), 531-537.

Kaur, D. & Sharma, R. (2012). An Update on Pharmacological Properties of Cumin. International Journal of Research in Pharmacy and Science, 2(4), 14-27.

Kavas, N. & Kavas, G. (2016). Physical-chemical and antimicrobial properties of Egg White Protein Powder films incorporated with orange essential oil on Kashar Cheese. Food Science and Technology Campinas, 36(4), 672-678.

King, A. H. (1983). Brown seaweed extracts (Alginates). Food Hydrocolloids, 2, 115-188.

Kirk, R. S. & Sawyer, R. (1991). Pearson's Composition and Analysis of Foods, 9th ed. Longman Scientific & Technical, Essex 643.

Koushki, M. R., Azizi, M. H., Koohy-Kamaly, P. & Azizkhani, M. (2015). Effect of Calcium Alginate Edible Coatings on Microbial and Chemical Properties of Lamb Meat during Refrigerated Storage. Journal of Food Quality and Hazards Control, 2, 6-10.

Lopez-Rubio, A., Fabra, M. J., Martinez-Sanz, M. & Mendoza, S. (2017). Biopolymer-Based Coatings and Packaging Structures for Improved Food Quality. Journal of Food Quality, 1-2.

Manohar, C. M., Kundgar, S. D. & Doble, M. (2017). Betanin immobilized LDPE as antimicrobial food wrapper. LWT-Food Science and Technology, doi: 10.1016/j.lwt.2016.07.020.

Margolies, B. J. & Barbano, D. M. (2018). Determination of fat, protein, moisture, and salt content of Cheddar cheese using mid-infrared transmittance spectroscopy. Journal of Dairy Science, 101(2), 924-933.

Mei, J., Yuan, Y., Wu, Y. & Li, Y. (2013). Characterization of edible starch–chitosan film and its application in the storage of Mongolian cheese. International Journal of Biological Macromolecules, 57, 17-21.

Miles, A., Misra, S. S. & Irwin, J. O. (1938). The estimation of the bactericidal power of the blood. Journal of Hygiene, 38, 732-749.

Najda, A., Dyduch, J. & Brzozowski, N. (2008). Flavonoid content and antioxidant activity of caraway roots (Carum carvi L.). Vegetable Crops Research Bulletin, 68, 127-33.

Pantaleao, I., Pintado, M. M. E. & Pocas, M. F. F. (2007). Evaluation of two packaging systems for regional cheese. Food Chemistry, 102, 481-487.

Perumalla, A. V. S. & Hettiarachchy, N. S. (2011). Green tea and grape seed extracts- potential applications in food safety and quality. Food Research International, 44, 827-839.

Rafei, S., Azizkhani, M. & Areaei, P. (2017). Impact of antioxidative properties of cumin and tarragon essential oils on the quality of full-fat white cheese. Journal of food technology and nutrition,14, 70-90.

Ramezani, M., Hosseini, S. M., Shahzadeh Fazeli, S. A. & Amozegar, M. A. (2017). PCR-DGGE analysis of fungal community in manufacturing process of a traditional Iranian cheese. Iranian Journal of Microbiology, 10(3), 180-186.

Ramos, Ó. L., Pereira, J. O., Silva, S. I., Fernandes, J. C., Franco, M. I., Lopes-da-Silva, A. & Malcata, F. X. (2012). Evaluation of antimicrobial edible coatings from a whey protein isolate base to improve the shelf life of cheese. Journal of Dairy Science, 95(11), 6282-6292.

Rhim, J. W. & Wang, L. F. (2013). Mechanical and water barrier properties of agar/carrageenan/konjac glucomannan ternary blend biohydrogel films. Carbohydrate Polymers, 96, 71-81.

Seydim, A. C. & Sarikus, G. (2006). Antimicrobial activity of whey protein-based edible films incorporated with oregano, rosemary, and garlic essential oils. Food Research International, 39, 639-644.

Strack, D., Vogt, T. & Schliemann, W. (2003). Recent advances in betalain research. Phytochemistry, 62, 247-269.

Wang, X. Y., Du, Y. M., Luo, J. W., Lin, B. F. & Kennedy, J. F. (2007). Chitosan/organic rectorite nanocomposite films: structure, characteristic, and drug delivery behavior. Carbohydrate Polymers, 69, 41-49.

Wang, L. F. & Rhim, J. W. (2015). Preparation and application of agar/alginate/collagen ternary blend functional food packaging films. International Journal of Biological Macromolecules, 80, 460-468.

Weerakkody, N. S., Caffin, N., Turner, M. S. & Dykes, G. A. (2010). In vitro antimicrobial activity of less-utilized spice and herb extracts against selected food-borne bacteria. Food Control, 21, 1408-1414.

Zhong, Y., Cavender, G. & Zhao, Y. (2013). Investigation of different coating application methods on the performance of edible coating on Mozzarella cheese. LWT – Food Science and Technology, 56, 1-8.