آنالیز ژنوم کلروپلاستی دو گونه دیپلوئید B. oleracea و B. rapa و دو گونه تتراپلوئید B. napus و B. juncea خانواده شب‌بویان (Brassicaceae) با استفاده از روش‌های بیوانفورماتیکی
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
1 - بخش تحقیقات علوم زراعی و باغی، مركز تحقیقات و آموزش كشاورزی و منابع طبیعي آذربایجان غربی، سازمان تحقیقات، آموزش و ترویج كشاورزی، ارومیه، ایران
2 - بخش تحقیقات علوم زراعی و باغی، مركز تحقيقات و آموزش كشاورزي و منابع طبيعي آذربايجان غربي، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران
کلید واژه: بیوانفورماتیک, براسیکا, کلروپلاست, شب‌بو, DNA,
چکیده مقاله :
امروزه، محبوبیت جنس براسیکا به دلیل ارزش غذایی و اثرات دارویی آن در حال افزایش است. این گروه شامل تعداد زیادی از غذاهای گیاهی مانند کلم، کلم بروکلی، گل کلم، خردل و همچنین دانه‌های روغنی کلزا است. در مطالعه حاضر، ژنوم کامل DNA کلروپلاست دو نوع دیپلوئید خانواده شب‌بو B. oleracea (C, n=9) و B. rapa (A, n=10) با دو نوع آلوتتراپلوئید B. napus (AC, n=19) و B. juncea (AB, n=18) مطالعه و ارزیابی شد. نتایج نشان داد در بین گونه‌های انتخاب شده، گونه B. napus با 152860 جفت باز دارای کوتاه‌ترین طول نوکلئوتیدی بوده و دو گونه B. rapa و B. juncea هرکدام با 153483 جفت باز دارای طول مساوی بوده و طول توالی نوکلئوتیدی در گونه B. oleracea برابر 153364 جفت باز می‌باشد. در بررسی نواحی کد کننده هر چهار ژنوم، مشخص شد که گونه B. napus با 79715 جفت باز در ناحیه CDS دارای کوتاه‌ترین طول توالی در این ناحیه و دو گونه B. rapa و B. juncea هر کدام با 80463 جفت باز در ناحیه CDS دارای طول بازی مساوی بوده و در مورد B. oleracea طول این ناحیه برابر 80559 جفت باز می‌باشد. نواحی غیر کد کننده اینترونی در گونه B. rapa با بیشترین‌ترین طول (30197 جفت باز) که فقط دو جفت باز با B. juncea (30195 جفت باز) اختلاف دارد. طول این ناحیه در B. oleracea برابر 30178 جفت باز بوده و گونه B. napus با 298660 جفت باز دارای کوتاه‌ترین طول می‌باشد.
Nowadays, the popularity of the genus Brassica is increasing due to its nutritional value and medicinal effects. This group includes a large number of vegetable foods such as cabbage, broccoli, cauliflower, mustard and rapeseed oil. In this study, the complete chloroplast DNA genome of two diploid species of the oleracea family, B. oleracea (C, n=9) and B. rapa (A, n=10) with two allotetraploid species B. napus (AC, n=19) and B. juncea (AB, n=18) was studied and evaluated. The results showed that among the selected species, B. napus has the shortest nucleotide length with 152860 bp, and two species B. rapa and B. juncea each have the same length with 153483 bp, and the length of the nucleotide sequence in B. oleracea species It is equal to 153364 bp. In the analysis of the coding regions of all four genomes, it was found that the species B. napus with 79715 bp in the CDS region has the shortest sequence length, and the two species B. rapa and B. juncea each with 80463 bp in the CDS region have the same length. B. oleracea, showed the length of this region equal to 80559 bp. Intronic non-coding regions in B. rapa species with the longest length (30197 bp) which is only two bp different from B. juncea (30195 bp). The length of this region in B. oleracea is equal to 30178 bp and B. napus has the shortest length with 298660 bp.
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