سنتز سبز نانوذرات طلاتوسط گیاه دارویی آویشن شیراز

محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم

تاریخ دریافت : 1393/10/20 تاریخ پذیرش : 1393/12/10 تاریخ انتشار : 1394/03/20

کلید واژه: نانوذره, طلا, سبز, گیاه, آویشن شیراز,

چکیده مقاله :

امروزه سنتز زیستی نانوذرات طلا مورد توجه قرار گرفته است. عصاره‌های گیاهی سازگار با محیط زیست هستند و با توجه به کاهش هزینه ها و عدم استفاده از مواد سمی برای سنتز نانوذرات مناسب می‌باشند. در پژوهشحاضر، روش سبز برای تولید نانوذرات طلا با استفاده ازعصاره برگ گیاه آویشن استفاده شده است عصاره آبیاز برگ گیاه دارویی آویشنشیرازتهیه شد. این عصاره با غلظت مختلف با محلول HAuCl4 مخلوط گردید. سنتز نانوذرات توسط تغییر رنگ محلول HAuCl4بررسی گردید، تشکیل نانوذره طلابا استفاده از طیف سنجی UV -Visو شکل و اندازه نانو ذرات با میکروسکوپ الکترونی عبوری بررسی شد، آنالیز انتشار انرژی اشعه ایکس برای تآیید حضور عنصر طلا در نمونه‌ها انجام شد. رنگمحلول HAuCl4 در ترکیب با عصاره گیاهی آویشن شیرار از زرد به سرخ یاقوتیتغییر یافت، این تغییر رنگ نشان دهنده سنتز نانوذرات طلا بود. طیف سنجی UV -Vis نشان داد که پیک جذبی حدود 540 نانومتر می‌باشد. تصاویر میکروسکوپ الکترونی گذاره نشان داد که نانوذرات در اشکال کروی، نامنظم و پنج وجهی می‌باشند.همچنین اندازه‌های نانو ذرات مختلف بین 50-1نانومتر تعیین شد. آنالیز انتشار انرژی اشعه ایکس پیک جذبی را در محدود 3/2 کیلو وات بدلیل وجود نانو ذرات طلا نشان می‌دهد. نتایج بیان گر آن است که می‌توان توسط عصاره آویشن شیراز نانو ذر ه ات طلا سازگار با محیط زیست، بدون استفاده از مواد شیمیایی نامناسب و خطرناک تولید کرد.

چکیده انگلیسی:

Biosynthesis of nanoparticle offers a vast studied today. Plant extracts are eco-friendly and cost effective to synthesis nanoparticles. In present study, has been presented a simple biosynthesis of gold nanoparticles usingZataria multiflora leaf’s extract. The  aqua extract from Zataria multiflora leaf’sextract was prepared. This extract in different concentration with HAuCl4 solution was mixed. Synthesized nanoparticle investigated by the change of color of auric chloride, and growth of nanoparticle in different time andconcentration was monitored by using UV-Vis Spectroscopy, transmission electron microscope (TEM) was used to analyze the shape and size nanoparticles, Energy-dispersive X-ray spectroscopy )EDS( analysis for evaluated present of gold element. The colour of HAuCl4 solution change from yellow in color to ruby red, this confirmed that gold nanoparticle was fabricated. UV-visible spectroscopyshowed the absorbance peak of gold nanoparticles occur around 540 nm in Zataria multiflora leaf’sextract. TEM images of sample revealed that the nanoparticles were spherical, pantagon and shaped with no definite morphology. This nanoparticle were different from 1-50 nm in size. EDS analysis results showed absorption peak approximately at 2.30 keV due to presence of gold nanoparticles. The results showed that gold nanoparticles canbe producedbyZataria multiflora leaf’sextract without usingenvironmentallyhazardouschemicalsunsuitable agent.

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