The effect of biosynthesized silver nanoparticles on FAE1 and FAD2 gene expression in Camelina sativa

Subject Areas : Plant Physiology

Tayebehalsadat Mirmoeini ¹ , Leila Pishkar ² , Danial Kahrizi ³ , Giti Barzin ⁴ , Naser Karimi ⁵

1 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
2 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran
3 - Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
4 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran.
5 - Plant Physiology Laboratory, Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran

Received: 2021-01-10 Accepted : 2021-06-13 Published : 2021-10-01

Keywords: nanoparticles, Real Time PCR, Gene expression, Biosynthesize, Camelina,

Abstract :

Nanotechnology is a field of research related to physics, chemistry, engineering sciences with the application of new techniques and production of nanoscale materials and an emerging field in interdisciplinary research especially biotechnology. Camelina is an oilseed and re-emerging plant that requires a lot of research on its oil production process. This study was conducted to investigate the effect of biosynthesized silver nanoparticles on the expression level of FAE1 and FAD2 in Soheil cultivar of Camelina oilseed plant based on a completely randomized design with four replications in 2018-2019. The aqueous extract of Camelina leaf and silver nitrate salt was used to prepare the nanoparticles. Experimental treatments included 0.5, 1, 2 and 3 mg / L of silver nanoparticles. After the preparation of foliar samples for all treatments, RNA extraction, cDNA synthesis and temperature gradient determination, the Real Time PCR reaction was used to study gene expression patterns. Data were then analyzed using GenEX and SAS software. The results showed that the effect of silver nanoparticles on FAE1 and FAD2 gene expression was significant (p<0.05) and the effect was increased with increasing silver nanoparticle concentration. The highest enhancement was observed at 3 mg / L silver nanoparticles.

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