Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer

Subject Areas : Microbiology

M. Shahmoradi ¹ , M. Rasouli ² , R. Footuhi Ghazvini ³ , A. Imani ⁴ , Y. Hamidogli ⁵

1 - Department of Horticultural science, Faculty of Agriculture, Guilan University, Rasht, Iran
2 - Department of Horticulture, Faculty of Agriculture, Malayer University, Malayer, Iran
3 - Department of Horticultural science, Faculty of Agriculture, Guilan University, Rasht, Iran
4 - Department of Horticultural, Seed and Plant Improvement Institute (SPII), Karaj 31587, Iran
5 - Department of Horticultural science, Faculty of Agriculture, Guilan University, Rasht, Iran

Received: 2015-10-08 Accepted : 2015-10-08 Published : 2013-09-01

Keywords: PCR, Almond, self-incompatibility, Sf-allele, S1-allele, SfF/SfR primer,

Abstract :

Self-incompatibility in almond and Prunus species is an important trait that prevents self-fertilization. Selfincompatibility in almond is controlled gametophytically by the multiallelic S-locus. Present study was done in order to identification and preliminary selection of self compatible progenies resulted from controlled crosses in almond using specific primer SfF/SfR. Some important morphological traits of parental crosses were evaluated using almond descriptor. Also, progenies (F1) of five crosses inculding; A (Tuono × 101 Genotype), B (Supernova × 101 Genotype), C (Genco × Shahrood 21), D (Tuono × Shahrood 12) and E (Tuono × shahrood 17) were tested using PCR in order to DNA amplification and self-compatibility evaluation. The result of PCR method found that using SfF/SfR pair primer, self-compatible progenies showed 449 bp bands, while this band not observed in self-incompatible progenies. In addition, all self- incompatible progenies appeared no S1 allele at any condition. According to Chi-square test, ratios of self-compatible to self-incompatible progenies were to Mendelian principles. It can be suggested that PCR method of this research has high potential in order to distinguish self-compatible and incompatible genotypes.