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Manuscript ID : 12843 Visit : 191 Page: 1 - 6

Article Type: Original Research

Evaluating the relationship of fungal contamination and ochratoxin A content in nonalcoholic beers from different climatic regions

Subject Areas :

Somayeh Alsadat Hosseini Khorami 1 , Reza Mahdavi 2 , Abolhasan Kazemi 3

1 - Department of Nutrition and dietetic, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia|Department of Nutrition and dietetic, Tabriz University of Medical Sciences, Tabriz, Iran
2 - Department of Nutrition and dietetic, Tabriz University of Medical Sciences, Tabriz, Iran
3 - Tropical and Infection Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Received: 2018-04-02 Accepted : 2018-06-09 Published : 2018-06-01

Keywords: Fungal contamination, Mycotoxin, Nonalcoholic beers, Ochratoxin A, &lt, i&gt, Aspergillus niger&lt, /i&gt, ,

Abstract :

Ochratoxin A (OTA) is a mycotoxin produced by some species of fungi such as Aspergillus and Penicillium. It is found as a contaminant in a variety of animal and human foods. Ochratoxin A has teratogenic, hepatotoxic, nephrotoxic, nephrocarcinogenic and immunosuppressive effects on human and animals. This study was carried out to evaluate the content of ochratoxin A in non-alcoholic beers, which were randomly collected from different retail outlets. All samples were analyzed for ochratoxin A by ELISA. Identification of fungal isolates was based on both macroscopic characters (colony growth, colony diameter) and microscopic characters. The tease Mount technique was used in this study and the fungi were cultivated in YGC (yeast glucose chloramphenicol agar) medium. All of the samples were contaminated with ochratoxin A but the levels of the contamination were below the maximum permitted levels. However, the difference between local and imported beer samples was not statistically significant (p>0.05). The mycological survey showed that 100% of domestic and imported beer samples were contaminated with Aspergillus, while 31.4% of the domestic and 40% of the imported beer samples were contaminated with Penicillium. Among the Aspergillus species, the most representative specie was Aspergillus niger. Although the ochratoxin A concentrations of non-alcoholic beers were under the European maximum permitted levels, the long-term continual consumption may have considerable health problem despite the low levels of contamination.

References:


  1. Cramer B, Harrer H, Nakamura K, Uemura D, Humpf H-U. Total synthesis and cytotoxicity evaluation of all ochratoxin A stereoisomers. Bioorganic & Medicinal Chemistry. 2010;18(1):343-7.
    2.
  2. Ringot D, Chango A, Schneider Y-J, Larondelle Y. Toxicokinetics and toxicodynamics of ochratoxin A, an update. Chemico-Biological Interactions. 2006;159(1):18-46.
    3.
  3. Zaied C, Bouaziz C, Azizi I, Bensassi F, Chour A, Bacha H, et al. Presence of ochratoxin A in Tunisian blood nephropathy patients. Exposure level to OTA. Experimental and Toxicologic Pathology. 2011;63(7-8):613-8.
    4.
  4. Wang Y, Liu J, Cui J, Xing L, Wang J, Yan X, et al. ERK and p38 MAPK signaling pathways are involved in ochratoxin A-induced G2 phase arrest in human gastric epithelium cells. 2012;209(2):186-92.
    5.
  5. Pfohl-Leszkowicz A. Ochratoxin A and aristolochic acid involvement in nephropathies and associated urothelial tract tumours. Toxicology Letters. 2009;60(4):465-83.
    6.
  6. Vettorazzi A, van Delft J, de Cerain AL. A review on ochratoxin A transcriptomic studies. Food and Chemical Toxicology. 2013;59:766-83.
    7.
  7. Thuvander A, Breitholtz-Emanuelsson A, Brabencova D, Gadhasson I. Prenatal exposure of Balb/c mice to ochratoxin A: effects on the immune system in the offspring. Food and Chemical Toxicology. 1996;34(6):547-54.
    8.
  8. Lioi M, Santoro A, Barbieri R, Salzano S, Ursini M. Ochratoxin A and zearalenone: a comparative study on genotoxic effects and cell death induced in bovine lymphocytes. Mutation Research/Genetic Toxicology and Environmental Mutagenesis.  2004;557(1):19-27.
    9.
  9. Djukanović L, Stefanović V, Basta-Jovanović G, Bukvić D, Glogova S, Dimitrijević J, et al. Investigation of Balkan endemic nephropathy in Serbia: how to proceed? Srpski arhiv za celokupno lekarstvo. 2010;138(3-4):256-61.
    10.
  10. Andrade PD, da Silva JLG, Caldas EDJ. Simultaneous analysis of aflatoxins B1, B2, G1, G2, M1 and ochratoxin A in breast milk by high-performance liquid chromatography/fluorescence after liquid–liquid extraction with low temperature purification (LLE–LTP). Journal of Chromatography A. 2013;1304:61-8.
    11.
  11. Duarte S, Pena A, Lino C. A review on ochratoxin A occurrence and effects of processing of cereal and cereal derived food products. Food Microbiology. 2010;27(2):1.87-98.
    12.
  12. Khalesi M, Khatib N. The effects of different ecophysiological factors on ochratoxin A production. Environmental Toxicology and Pharmacology. 2011;32(2):113-21.
    13.
  13. Mateo R, Medina Á, Mateo EM, Mateo F, Jiménez M. An overview of ochratoxin A in beer and wine. International Journal of Food Microbiology. 2007;119(1-2):79-83.
    14.
  14. Abarca M, Bragulat M, Castella G, Cabanes FJAEM. Ochratoxin A production by strains of Aspergillus niger var. niger. Applied and Environmental Microbiology. 1994;60(7):2650-2.
    15.
  15. Téren J, Varga J, Hamari Z, Rinyu E, Kevei F. Immunochemical detection of ochratoxin A in black Aspergillus strains. Mycopathologia. 1996;134(3):171-6.
    16.
  16. Abarca M, Accensi F, Bragulat M, Cabañes FJJ. Current importance of ochratoxin A–producing Aspergillus spp. Journal of Food Protection. 2001;64(6):903-6.
    17.
  17. Jorgensen K. Survey of pork, poultry, coffee, beer and pulses for ochratoxin A. Food Additives and Contaminants. 1998;15(5):550-4.
    18.
  18. Kuiper-Goodman T, Scott PJB, BES es. Risk assessment of the mycotoxin ochratoxin A. Food Additives and Contaminants. 1989;2(3):179-248.
    19.
  19. Micco C, Grossi M, Miraglia M, Brera C. A study of the contamination by ochratoxin A of green and roasted coffee beans. Food Additives and Contaminants. 1989;6(3):333-9.
    20.
  20. Pietri A, Bertuzzi T, Pallaroni L, Piva IVG. Occurrence of ochratoxin A in Italian wines. Food Additives and Contaminants. 2001;18(7):647-54.
    21.
  21. Sage L, Krivobok S, Delbos É, Seigle-Murandi F, Creppy EEJ. Fungal flora and ochratoxin A production in grapes and musts from France. Journal of Agricultural and Food Chemistry. 2002;50(5):1306-11.
    22.
  22. Zimmerli B, Dick R. Ochratoxin A in table wine and grape‐juice: occurrence and risk assessment. Food Additives and Contaminants. 1996;13(6):655-68.
    23.
  23. Medina Á, Jiménez M, Gimeno-Adelantado JV, Valle-Algarra FM, Mateo RJ. Determination of ochratoxin A in beer marketed in Spain by liquid chromatography with fluorescence detection using lead hydroxyacetate as a clean-up agent. Journal of Chromatography A. 2005;1083(1-2):7-13.
    24.
  24. Odhav B, Naicker V. Mycotoxins in South African traditionally brewed beers. Food Additives and Contaminants. 2002;19(1):55-61.
    25.
  25. Visconti A, Pascale M, Centonze GJ. Determination of ochratoxin A in domestic and imported beers in Italy by immunoaffinity clean-up and liquid chromatography. Journal of Chromatography A. 2000;888(1-2):321-6.
    26.
  26. Maurya SP, Prakash PY, Bairy IJ. A simplified touch tape preparation from tube cultures for microscopic examination of filamentous fungi. Journal of Microbiological Methods. 2011;86(1):128-9.
    27.
  27. Ibáñez-Vea M, Lizarraga E, González-Peñas E, de Cerain AL. Co-occurrence of type-A and type-B trichothecenes in barley from a northern region of Spain. Food Control. 2012;25(1):81-8.
    28.
  28. Esteban A, Abarca ML, Bragulat MR, Cabañes FJ. Effects of temperature and incubation time on production of ochratoxin A by black aspergilli. Research in Microbiology. 2004;155(10):861-6.
    29.
  29. Gumus T, Arici M, Demirci MJ. A survey of barley, malt and beer contamination with ochratoxin A in Turkey. Journal of the Institute of Brewing. 2004;110(2):146-9.
    30.
  30. Magan N, Aldred D. Conditions of formation of ochratoxin A in drying, transport and in different commodities. Food Additives and Contaminants. 2005;22(s1):10-6.
    31.
  31. Kralj Cigić I, Prosen H. An overview of conventional and emerging analytical methods for the determination of mycotoxins. International Journal of Molecular Sciences. 2009;10(1):62-115.
    32.

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