تاثیر چاقی و مکمل‌یاری عصاره سیر درکنار فعالیت هوازی بر بیان ژن‌های فاکتور نورون‌زایی مشتق‌شده از مغز، تیروزین کینازB و حافظه فضائی کوتاه مدت رت‌های نر ویستار

محورهای موضوعی : فصلنامه زیست شناسی جانوری

بهروز اسفندیاری ¹ , رضا رضائی شیرازی ² , سیدجواد ضیاءالحق ³ , سعید قربانی ⁴ , حبیب اصغرپور ⁵

1 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
3 - گروه تربیت بدنی و علوم ورزشی، واحد شاهرود، دانشگاه آزاد اسلامی، شاهرود، ایران
4 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران
5 - گروه تربیت بدنی و علوم ورزشی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران

تاریخ دریافت : 1400/08/30 تاریخ پذیرش : 1400/11/02 تاریخ انتشار : 1401/06/01

کلید واژه: چاقی, عصاره سیر, اختلال شناختی, ماز Y, فعالیت بدنی هوازی,

چکیده مقاله :

میزان اختلال عملکرد شناختی در چاقی چند سالی است بسیار مورد تحقیق قرار گرفته‌است. همچنین تاثیر عصاره سیر و فعالیت هوازی بر این اختلالات شناختی در افراد چاق همچنان ناشناخته است. 40 سر رت نر ویستار جهت القاء چاقی به مدت 12 هفته رژیم غذایی پرچرب قرار گرفتند و در نهایت 30 سر رت چاق با استفاده از شاخص Lee شناسایی شده و سپس به طور تصادفی به 5 گروه کنترل، چاق، سیر، تمرین هوازی، سیر+ تمرین هوازی تقسیم شدند. تمرینات هوازی شامل30 دقیقه در روز، m/min 8 و 5 روز در هفته و عصاره سیر و استویا با غلظت mg/kg 250 نیز به آب مصرفی روزانه اضافه شد. بافت مغز جهت مطالعات بیان ژن به روش real-time PCR به آزمایشگاه ارسال شد. از آزمون ANOVA یکطرفه و آزمون تعقیبی LSD جهت تعیین اختلاف بین گروه‌ها استفاده و سطح معنی‌داری 0.05 درنظر گرفته شد. نتایج نشان داد در مقایسه با گروه کنترل، 12 هفته تغذیه پرچرب، وزن بدن موش‌های صحرائی را افزایش داد (05/0 > p) و حتی در طول مداخلات عصاره‌ای 8 هفته ای و تمرینات، همچنان نسبت به گروه کنترل بالاتر باقی ماند. بعلاوه مقادیر بیان ژن BDNF کاهش غیرمعنی‌دار داشته (05/0 < p) و مقادیر بیان ژن TrKB، نیز در 12 هفته رژیم پرچرب در موش‌های صحرائی، نسبت به گروه کنترل سالم، افزایش غیرمعنی‌دار داشت (05/0 < p). از طرفی، در ماز Y، درصد تناوب در موش‌های صحرائی چاق به طورمعنی‌داری کمتر از گروه کنترل بود (05/0 >p ). القاء رژیم پرچرب در کودکی موجب تغییرات خفیف ژنی در موش‌های صحرائی نر ویستار شده و همچنین بنظر می‌رسد ترکیب فعالیت های بدنی هوازی و عصاره‌ای موثر تر از مداخله‌های هوازی و عصاره‌ای به تنهائی باشد.

چکیده انگلیسی:

The extent of cognitive impairment in obesity has been extensively researched for several years. Also, the effect of garlic extract and aerobic activity on these cognitive disorders in obese people is still unknown. Forty male Wistar rats were exposed to a high-fat diet for 12 weeks to induce obesity, and finally 30 obese rats were identified using the Lee index and then randomly divided into 5 groups: control, obesity, garlic, aerobic exercise, garlic + exercise. Were aerobically divided. Aerobic exercise including 30 minutes per day, 8 / m / min 5 days a week and garlic and stevia extract at a concentration of 250 mg / kg were added to the daily water intake. Brain tissue was sent to the laboratory to study gene expression by real-time PCR. One-way ANOVA and LSD post hoc test were used to determine the differences between groups and a significance level of 0.05 was considered. The results showed that compared to the control group, 12 weeks of high-fat diet increased the body weight of rats (p <0.05) and even during 8-week extract interventions and training, remained higher than the control group. . In addition, BDNF gene expression levels decreased significantly (P <0.05) and TrKB gene expression levels also increased significantly in 12 weeks of high-fat rats compared to the healthy control group (p <0.05). On the other hand, in the Y maze, the percentage of rotation in obese rats was significantly lower than the control group (p <0.05). Induction of a high-fat diet in childhood causes mild genetic changes in male Wistar rats, and the combination of aerobic and extractive physical activity appears to be more effective than aerobic and extractive interventions alone.

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