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کد مقاله : 543125 بازدید : 100 صفحه: 9 - 18

10.22034/aej.2018.543125

نوع مقاله: پژوهشی

اثر محلول‎پاشی متانول و اتانول بر ترکیبات اسانس نعناع‌فلفلی

محورهای موضوعی : بوم شناسی گیاهان زراعی

حسن نورافکن 1 , زهرا کلانتری 2 , فاطمه سفیدکن 3

1 - گروه علوم باغبانی، دانشکده کشاورزی، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران
2 - گروه علوم باغبانی، دانشکده کشاورزی، واحد میانه، دانشگاه آزاد اسلامی، میانه، ایران
3 - مؤسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

تاریخ دریافت : 1396/11/08 تاریخ پذیرش : 1397/04/21 تاریخ انتشار : 1397/06/01

کلید واژه:  تغذیه برگی,  دی‌اکسیدکربن,  منتول,  منتون,  هیدروالکل,

چکیده مقاله :

گونه های مختلف نعناع منبع عمده تولید اسانس های منتولی به شمار می روند. محلول پاشی گیاهان دارویی با الکل هایی نظیر متانول و اتانول می تواند افزایش رشد و میزان متابولیت های ثانویه را سبب شود. این پژوهش جهت تعیین اثر محلول‎پاشی متانول و اتانول بر ترکیب اسانس نعناع فلفلی در مزرعه تحقیقاتی گیاهان دارویی دانشگاه آزاد اسلامی واحد میانه به صورت آزمایشی در قالب طرح بلوک های کامل تصادفی انجام گرفت. گیاهچه های 1 ماهه نعناع فلفلی با محلول های آبی 10، 20، 30 و 40% حجمی متانول و اتانول محلول پاشی شده و این کار 2 و 4 هفته بعد از اولین محلول پاشی تکرار شد. گیاهان در مرحله گلدهی کف بُر شده و استخراج اسانس با روش تقطیر با آب انجام شد. ترکیب اسانس نعناع فلفلی با دستگاه کروماتوگراف گازی و کروماتوگراف گازی متصل به طیف سنج جرمی تعیین شد. میزان ترکیبات موجود در اسانس نعناع فلفلی به جز ترانس پولِگُل، سیس پولِگُل، منتیل استات، ای کریوفیلن، آلفاهیموکالن و جرماکرن دی تحت تأثیر محلول پاشی با هیدروالکل ها قرار گرفت. کاربرد هیدروالکل ها در غلظت بالاتر از 10% میزان منتول را به طور معنی داری افزایش داد. در مجموع، میزان منتول در گیاهان محلول پاشی شده با متانول بیشتر از اتانول بود. با این حال، مقادیر اغلب ترکیبات موجود در اسانس نعناع فلفلی با کاربرد اتانول 30% افزایش بیشتری نشان داد. همچنین، محلول پاشی با متانول اثر منفی بر تولید منتون و ایزومنتون در نعناع فلفلی داشت. بنابراین، با توجه به یافته های این پژوهش، محلول پاشی متانول برای افزایش کیفیت اسانس نعناع فلفلی قابل توصیه می باشد.

چکیده انگلیسی:

Mentha spp. are the main source of menthol essential oils. Medicinal plants sprayed with alcohols such as methanol and ethanol may cause vegetative growth and secondary metabolites improvement. The current study was carried out to evaluate ethanol and methanol foliar application efficiency on peppermint essential oils composition at Medicinal Plant Research Farm of Islamic Azad University, Miyaneh Branch based on randomized complete block design. One month aged peppermint plantlets were sprayed by 10, 20, 30 and 40% (v/v) ethanol and methanol aqueous solutions repeated twice two and four weeks after. The plants were harvested in flowering stage and their essential oils were extracted using hydro-distillation. The essential oils composition was specified using GC and GC-MS techniques. All phytochemicals in peppermint essential oils were significantly affected by hydroalcoholic solutions application but transpulegol, cis-pulegol, menthyl acetate, e-caryophyllene, α-himochalene and germacrene D. Plants sprayed by upper than 10% hydroalcohol solutions contained more menthol, significantly. Moreover, menthol amount was in higher values in the case of methanol application than ethanol. However, most of phytochemicals of peppermint were effectively promoted using ethanol 30%. Also, menthone and isomenthone negatively affected by methanol spraying in peppermint. Therefore, considering current research findings, methanol spraying is recommending to increase peppermint essence quality.

منابع و مأخذ:


  1. Abdolmaleki M, Bahraminejad S, Salari M, Abbasi S, Panjeke N (2011) Antifungal activity of peppermint (Mentha piperita L.) on phytopathogenic fungi. Journal of Medicinal Plants 2(38): 26-34. [in Persian with English abstract]
    2.
  2. Alaei SH, Khan Ahmadi M, Zaji B (2007) Comparative studies of essential oil and menthol content of cultivated peppermint in three different regions of Kermanshah. Plant and Ecosystem 3(9): 71-81. [in Persian with English abstract]
    3.
  3. Asgari AA, Moinfard A (2014) The effect of alcohol foliar application on plants as a modern application in agriculture. Proceedings of the First National Congress of Biology and Natural Sciences of Iran. Tehran, Iran 4 pp. [in Persian]
    4.
  4. Chakraborty A, Chattopadhyay S (2008) Stimulation of menthol production in Mentha piperita cell culture. In Vitro Cellular and Developmental Biology – Plant 44(6): 518-524.
    5.
  5. Fall R, Benson AA (1996) Leaf methanol, the simplest natural product from plants. Trends Plant Sciences 1(9): 296-301.
    6.
  6. Farzaneh A, Ghani A, Azizi M (2010) The effect of water stress on morphological characteristic and essential oil content of improved sweet basil (Ocimum basilicum L.). Journal of Plant Production 17(1): 103-111. [in Persian with English abstract]
    7.
  7. Heidari F, Zehtab Salmasi S, Javanshir A, Aliari H, Dadpoor MR (2008a) The effects of application microelements and plant density on phonology, yield and essential oil of peppermint (Mentha piperita L.). Iranian Journal of Medicinal and Aromatic Plants 24(1): 1-9. [in Persian with English abstract]
    8.
  8. Heidari F, Zehtab Salmasi S, Javanshir A, Aliari H, Dadpoor MR (2008b) The effect of plant density on yield and production of essential oil of peppermint (Mentha piperita L.). Journal of Water and Soil Science 12(45): 501-510. [in Persian with English abstract]
    9.
  9. Izadi Z, Ahmadvand GH, Esna-Ashari M, Piri M (2010) The effect of nitrogen and plant density on some growth characteristics, yield and essential oil in peppermint (Mentha piperita L.). Iranian Journal of Field Crops Research 8(5): 824-836. [in Persian with English abstract]
    10.
  10. Khosravi MT, Mehrafarin A, Naghdibadi H, Hajiaghaee R, Khosravi E (2011) Effect of methanol and ethanol application on yield of Echinacea purpurea L. in Karaj region. Journal of Herbal Drugs 2(2): 121-128. [in Persian with English abstract]
    11.
  11. Mehrafarin A, Naghdi Badi H, Poorhadi M, Hadavi E, Qavami N, Kadkhoda Z (2011) Phytochemical and agronomical response of peppermint (Mentha piperita L.) to bio-fertilizers and urea fertilizer application. Journal of Medicinal Plants 4(40): 107-118. [in Persian with English abstract]
    12.
  12. Nonomura AM, Benson AA (1992) The path of carbon in photosynthesis: improved crop yield with methanol. Proceedings of the National Academy of Sciences of the United States of America. Boston, USA 9794-9798.
    13.
  13. Nourafcan H (2014) Elicitors, precursors and culture medium components effects on some growth characteristics, citral percentage and antimicrobial activity of essential oil of lemon verbena (Lippia citriodora H.B.K.) in vitro and greenhouse conditions. PhD Thesis, Islamic Azad University - Science and research Branch, Faculty of Agriculture: Tehran, Iran. [in Persian with English abstract]
    14.
  14. Nourafcan H (2015) Effect of salicylic acid on salinity stress tolerance improvement of peppermint (Mentha piperita L.) in greenhouse. Agroecology Journal 10(2-2): 85-95. [in Persian with English abstract]
    15.
  15. Nourafcan H, Kalantari Z (2017) The effect of methanol and ethanol foliar application on peppermint morpho-physiological traits. Agroecology Journal 12(4): 1-9. [in Persian with English abstract]
    16.
  16. Safarzadeh Vishkaei MT (2007) Effect of methanol on growth and yield of peanut (Arachis hypogaea L.). PhD Thesis, Islamic Azad University - Science and research Branch, Faculty of Agriculture: Tehran, Iran. [in Persian with English abstract]
    17.
  17. Sajedi Moghadam S, Mehrafarin A, NaghdiBadi H, Pazoki AR, Qavami N (2012) Evaluation of phytochemical yield of thyme (Thymus vulgaris L.) under foliar application of hydro alcohols. Journal of Medicinal Plants 11(44): 130-140. [in Persian with English abstract]
    18.
  18. Sayyadi A, Ahmadi J, Asghari B, Hosseini SM (2014) Effect of drought stress and salinity on phenolic compounds of Thymus vulgaris L. Eco-phytochemical Journal of Medicinal Plants 2(4): 50-61. [in Persian with English abstract]
    19.
  19. Tahami Zarandi SMK, Rezvani Moghaddam P, Jahan M (2010) Comparison the effect of organic and chemical fertilizers on yield and essential oil percentage of basil (Ocimum basilicum L.). Agroecology 2(1): 63-74. [in Persian with English abstract]
    20.
_||_

  1. Abdolmaleki M, Bahraminejad S, Salari M, Abbasi S, Panjeke N (2011) Antifungal activity of peppermint (Mentha piperita L.) on phytopathogenic fungi. Journal of Medicinal Plants 2(38): 26-34. [in Persian with English abstract]
    2.
  2. Alaei SH, Khan Ahmadi M, Zaji B (2007) Comparative studies of essential oil and menthol content of cultivated peppermint in three different regions of Kermanshah. Plant and Ecosystem 3(9): 71-81. [in Persian with English abstract]
    3.
  3. Asgari AA, Moinfard A (2014) The effect of alcohol foliar application on plants as a modern application in agriculture. Proceedings of the First National Congress of Biology and Natural Sciences of Iran. Tehran, Iran 4 pp. [in Persian]
    4.
  4. Chakraborty A, Chattopadhyay S (2008) Stimulation of menthol production in Mentha piperita cell culture. In Vitro Cellular and Developmental Biology – Plant 44(6): 518-524.
    5.
  5. Fall R, Benson AA (1996) Leaf methanol, the simplest natural product from plants. Trends Plant Sciences 1(9): 296-301.
    6.
  6. Farzaneh A, Ghani A, Azizi M (2010) The effect of water stress on morphological characteristic and essential oil content of improved sweet basil (Ocimum basilicum L.). Journal of Plant Production 17(1): 103-111. [in Persian with English abstract]
    7.
  7. Heidari F, Zehtab Salmasi S, Javanshir A, Aliari H, Dadpoor MR (2008a) The effects of application microelements and plant density on phonology, yield and essential oil of peppermint (Mentha piperita L.). Iranian Journal of Medicinal and Aromatic Plants 24(1): 1-9. [in Persian with English abstract]
    8.
  8. Heidari F, Zehtab Salmasi S, Javanshir A, Aliari H, Dadpoor MR (2008b) The effect of plant density on yield and production of essential oil of peppermint (Mentha piperita L.). Journal of Water and Soil Science 12(45): 501-510. [in Persian with English abstract]
    9.
  9. Izadi Z, Ahmadvand GH, Esna-Ashari M, Piri M (2010) The effect of nitrogen and plant density on some growth characteristics, yield and essential oil in peppermint (Mentha piperita L.). Iranian Journal of Field Crops Research 8(5): 824-836. [in Persian with English abstract]
    10.
  10. Khosravi MT, Mehrafarin A, Naghdibadi H, Hajiaghaee R, Khosravi E (2011) Effect of methanol and ethanol application on yield of Echinacea purpurea L. in Karaj region. Journal of Herbal Drugs 2(2): 121-128. [in Persian with English abstract]
    11.
  11. Mehrafarin A, Naghdi Badi H, Poorhadi M, Hadavi E, Qavami N, Kadkhoda Z (2011) Phytochemical and agronomical response of peppermint (Mentha piperita L.) to bio-fertilizers and urea fertilizer application. Journal of Medicinal Plants 4(40): 107-118. [in Persian with English abstract]
    12.
  12. Nonomura AM, Benson AA (1992) The path of carbon in photosynthesis: improved crop yield with methanol. Proceedings of the National Academy of Sciences of the United States of America. Boston, USA 9794-9798.
    13.
  13. Nourafcan H (2014) Elicitors, precursors and culture medium components effects on some growth characteristics, citral percentage and antimicrobial activity of essential oil of lemon verbena (Lippia citriodora H.B.K.) in vitro and greenhouse conditions. PhD Thesis, Islamic Azad University - Science and research Branch, Faculty of Agriculture: Tehran, Iran. [in Persian with English abstract]
    14.
  14. Nourafcan H (2015) Effect of salicylic acid on salinity stress tolerance improvement of peppermint (Mentha piperita L.) in greenhouse. Agroecology Journal 10(2-2): 85-95. [in Persian with English abstract]
    15.
  15. Nourafcan H, Kalantari Z (2017) The effect of methanol and ethanol foliar application on peppermint morpho-physiological traits. Agroecology Journal 12(4): 1-9. [in Persian with English abstract]
    16.
  16. Safarzadeh Vishkaei MT (2007) Effect of methanol on growth and yield of peanut (Arachis hypogaea L.). PhD Thesis, Islamic Azad University - Science and research Branch, Faculty of Agriculture: Tehran, Iran. [in Persian with English abstract]
    17.
  17. Sajedi Moghadam S, Mehrafarin A, NaghdiBadi H, Pazoki AR, Qavami N (2012) Evaluation of phytochemical yield of thyme (Thymus vulgaris L.) under foliar application of hydro alcohols. Journal of Medicinal Plants 11(44): 130-140. [in Persian with English abstract]
    18.
  18. Sayyadi A, Ahmadi J, Asghari B, Hosseini SM (2014) Effect of drought stress and salinity on phenolic compounds of Thymus vulgaris L. Eco-phytochemical Journal of Medicinal Plants 2(4): 50-61. [in Persian with English abstract]
    19.
  19. Tahami Zarandi SMK, Rezvani Moghaddam P, Jahan M (2010) Comparison the effect of organic and chemical fertilizers on yield and essential oil percentage of basil (Ocimum basilicum L.). Agroecology 2(1): 63-74. [in Persian with English abstract]
    20.

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