بررسی خصوصیات فیزیولوژیکی و فعالیت آنتی‌اکسیدانی ریحان (Ocimum basilicum cv. Keshkeni luvelou)تحت سطوح مختلف متیلجاسمونات و سمیت سرب
الموضوعات :
سمیه اسدی
1
,
محمد مقدم
2
,
عبدالله قاسمی پیربلوطی
3
,
امیر فتوت
4
1 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
2 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهدف مشهد، ایران.
3 - مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهرقدس، تهران، ایران
4 - گروه علوم خاک، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
الکلمات المفتاحية: پرولین, محتوای نسبی آب, رنگیزه‌های فتوسنتزی, تنظیم کننده رشد, نشت الکترولیت,
ملخص المقالة :
آزمایشی به منظور بررسی تاثیر متیل جاسمونات بر خصوصیات فیزیولوژیکی و فعالیت آنتی اکسیدانی گیاه ریحان تحت سمیت سرب بصورت فاکتوریل در قالب طرح کاملا ً تصادفی در سه تکرار در گلخانه تحقیقاتی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل سه سطح نیترات سرب (صفر (شاهد)، 200 و 400 میلی گرم در کیلوگرم خاک) و محلول پاشی متیل جاسمونات در سه غلظت (صفر، 5/0 و 1 میلی‌مولار) بودند. صفات مورد ارزیابی شامل میزان کلروفیل a و b، کلروفیل کل، کارتنوئیدها، نشت الکترولیت، رطوبت نسبی، فعالیت آنتی‌اکسیدانی، کربوهیدرات های محلول، فنل کل و پرولین بود. نتایح حاصل از این مطالعه نشان داد که در گیاهان تیمار شده با سرب، میزان پرولین، محتوای نسبی آب، کلروفیلa ، فعالیت آنتی اکسیدانی، فنل کل در مقایسه با گیاهان شاهد کاهش معنی داری نشان داد و کاربرد متیلجاسمونات (5/0 میلی مولار) در شرایط تنش سرب، باعث افزایش این صفات گردید. علاوه بر این در شرایط تنش سرب، میزان نشت الکترولیت به میزان قابل توجهی افزایش یافت و محلول پاشی متیل جاسمونات سبب کاهش آن شد. بنابراین، استفاده از متیلجاسمونات برای حفظ عملکرد اقتصادی گیاهان تحت تنش قابل توجیه است. همچنین استفاده از غلظت‌های 5/0 و 1 میلی مولار آن در شرایط تنش عنصر سنگین سرب با تحریک ساخت آنتی اکسیدانت ها، منجر به کاهش و تعدیل اثرات این تنش می شود.
Abdala, G., Miersch, O., Kramell, R., Vigliocco, A., Agostini, E., Forchetti, G. and Alemano, S. (2003). Jasmonate and octadecanoid occurrence in tomato hairy roots. Endogenous level changes in response to NaCl. Plant Growth Regulation. 40: 21-27.
Al-Amier, H. and Craker, L. E. (2007). In-vitro selection for stress tolerant spearmint. Issues in New Crops and New Uses, pp. 306-310.
Aldoobie, N.F. and Beltagi, M.S. (2013). Physiological, biochemical and molecular responses of common bean (Phaseolus vulgaris L.) plants to heavy metals stress. African Journal of Biotechnology. 12(29): 4614-4622.
Amirjani, M., Abnosi, M.H., Mahdieh, M. and Ghareshekhlou, S. (2015). Study of the effect of lead on the activity of antioxidant enzymes, proline and total alkaloids of callus of Catharantus roseus. Cell and Tissue Journal. 6(1): 9-21. (In Persian).
Arabaci, D. and Bayram, E. (2004). The effect of nitrogen fertilization and different plant densities on some agronomic and technologic characteristic of Ocimum basilicum L. (Basil). Journal of Plant Biology Research. 3(4): 255-62.
Arora, A., Byrem, T.M., Nair, M. and Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics. 373:102–109.
Asadi Karam, A., Asrar, Z. and Keramat, B. (2013). Effect of methyl-jasmonate treatment on the content of phenolic compounds and PAL activity in Lepidium sativum under the toxicity of copper. Journal of Plant Process and Function. 2(6): 89-96. (In Persian).
Ayala-Zavala J.F., Wang, S.Y., Wang, C.Y. and Gonzalez-Aguilar, G.A. (2005). Methyl jasmonate in conjunction with ethanol treatment increases antioxidant capacity, volatile compounds and post-harvest life of straw berry fruit. European Food Research and Technology. 221: 731-8.
Babst, B.A., Ferrieri, R.A., Gray, D.W., Lerdau, M., Schyler, D.J., Schueller, M., Thorpe, M.R. and Orians, C.M. (2005). Jasmonic acid induces rapid changes in carbon transport and partitioning in populus. New Phytologist. 167: 63-72.
Baker, A.J.M., Mc Grath, S.P., Reeves, R.D. and Smith, J.A.C. (2000) . Metal hyperaccumulator plants: a review of the ecology and physiology of a biological resource for phytoremediation of metal-polluted soils. In: Phytoremediation of contaminated soil and water (eds. Terry, N. and Banuelos, G.). CRC Press, Boca Raton, Florida, USA. 85-107.
Barrientos Carvacho, H., Pérez, C., Zúñiga, G. and Mahn, A. (2014). Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts. Journal of the Science Food and Agriculture. 63(1): 20-31.
Bates, L.S., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1): 205-207.
Beladi, S.M., Kashani, A., Habibi, D., Paknejad, F. and Golshan, M. (2010). The effect of lead and copper on some physiological traits of Lathyrus sativus L. New Finding in Agriculture. 5(1): 9-20. (In Persian).
Brunet, J., Repellin, A., Varrault, G., Terrync, N. and Zuily-fodil, Y. (2008). Lead accumulation in the roots of grass pea (Lathyrus sativus): a novel plant for phytoremediation systems. Comptes Rendus Biologies. 331: 859-864.
Bruni, R. and Sacchetti, G. (2009). Factors affecting polyphenol biosynthesis in wild and field grown St. John´s Wort (Hypericum perforatum L. Hypericaceae/ Guttiferael). Molecules. 14: 682-725.
Capitani, F., Biondi, S., Falasca, G., Ziosi, V., Balestrazzi, A., Carbonera, D., Torrigiani, P. and Altamura, M. (2005). Methyl jasmonate disrupts shoot formation in tobacco thin cell layers by over-inducing mitotic activity and cell expansion. Planta. 220: 507-519.
Chen, J., Zhu, C., Lin, D. and Sun, Z. X. (2007). The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings. Canadian Journal of Plant Science. 87: 49-57.
Chien, H.F., Wang, J.W., Lin, C.C. and Kao C.H. (2001). Cadmium toxicity of rice leaves is mediated through lipid peroxidation. Journal of Plant Growth Regulation. 33: 205-213.
Choudhury, S. and Panda, S.K. (2004). Role of salicylic acid in regulating cadmium induced oxidative stress in Oryza sativa L roots. Bulgarian Journal of Plant Physiology. 30: 95-110.
Comparot, S.M., Graham, C.M. and Reid, D.M. (2002). Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Journal Plant Growth Regulation. 38: 21-30.
Creelman, R. A. and Mullet, J. E. (1997). Oligosacchrins, brassinolides and jasmonates: non traditional regulators of plant growth, development, and gene expression. American Society of Plant Biology. 9: 1211- 1223.
Divya, P., Puthusseri, B. and Neelwarne, B. (2013). The effect of plant regulators on the concentration of caretonoids and phenolic compound in foliage o coriander. LWT-Food Science and Technology. 56(1):101-110.
Dong, J., Wan, G. and Liang, Z. (2010). Accumulation of salicylic acid induced phenolic compounds and raised activities of secondary metabolic and antioxidant enzymes in Salvia miltorrhiza cell culture. Journal of Biotechnology. 148: 99-104.
Garcia-Mata, C. and Lamattina, L. (2001). Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiology. 126(3): 1196-1204.
Ghelich, S., Zarin Kamar, F. and Niknam, V. (2015). Investigating the amount of lead accumulation and its effect on peroxidase activity, phenolic and flavonoids content in germination stage in alfalfa (Medicago sativa L.). Journal of Plant Researches. 28(1): 164-174. (In Persian).
Gonzalez-Aguilar, G., Tiznado-Hernandez, M.E. and Zavaleta-Gaticar Martinez-Tellez, M.A. (2004). Methyl jasmonat treatment reduce chilling injury and activate the defense of guava fruits. Biochemical and Biophysical Research Communications. 313: 694-701.
Hashemi, Sh., Asrar, Z. and Pourseyidi, Sh. (2010). Effect of seed premixing by salicylic acid on growth and some physiological and biochemical indices in cress. Journal of Plant Researches. 2(2):1-10. (In Persian).
Heredia, J.B. and Cisneros-Zevallos, L. (2009). The effect of exogenous ethylene and methyl jasmonate on PAL activity, phenolic profiles and antioxidant capacity of carrots (Daucus carota) under different wounding intensities. Postharvest Biology and Technology, 51(2): 242-249.
Horton, R.F. (1991). Methyl Jasmonate and transpiration in barley. Plant Physiology. 96:1376-1378.
Islam, E., Yang, X., Li T., Liu D., Jin, X. and Meng, F. (2007). Effect of Pb toxicity on root morphology, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. Journal of Hazardous Materials. 147(3): 806–16.
John, R., Ahmad, P., Gadgil, K. and Sharma, S. (2009). Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. International Journal of Plant Diseases and Protection. 3(3): 1735-8043.
Jung, S. (2004). Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant Physiology and Biochemistry. 42: 231-255.
Kang, H.M. and Saltveit, M.E. (2002). Antioxidant enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles. Journal of Agricultural and Food Chemistry. 50(3): 513-518.
Kim, Y. Y., Yang, Y., and Lee, Y. (2002). Pb andCd uptake in rice roots. Plant Physiology. 116: 368-372.
Kovacik, J., Klejdus, B., Hedbavny, J., Covska, S. and Zon, J. (2010). Significance of phenols in cadmium and nickel uptake. Journal of Plant Physiology. 168: 576-584.
Kumar, A. and Elston, J. (1992). Genotypic differences in leaf water relations between Brassica juncea and B. napus. Annals of Botany. 70(1): 3-9.
Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi, J., Sriranganayakulu, G., Reddy, P.C. and Sudhakar, C. (2006). Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings. Biologia Plantarum. 50: 119-226.
Liamas, A., Ullrich, C.I. and Sanz, A. (2000). Cadmium effects on transmembrane electrical potential difference, respiration and membrane permeability of rice (Oryza sativa) roots. Plant and Soil. 219: 21-8.
Lutts, S., Kinet, J.M. and Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany. 46(12): 1843-1852.
Lutts, S., Kinet, J.M. and Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany. 78(3): 389-398.
Maciejewska, W. and Krupa, Z. (2002). Jasmonic acid and heavy metals in Arabidopsis plant a similar physiological response to both stressors. Plant Physiology. 159: 509-515.
Maksymiec, W. and Krupa, Z. (2006). The effects of shortterm exposition to Cd, excess Cu ions and jasmonate on oxidative stress appearing in Arabidopsis thaliana. Environmental and Experimental Botany. 57: 187-194.
Mazaheri Tirani, M., Kalantari, Kh. and Hasibi, N. (2008). Study of the interaction of ethylene and salicylic acid on oxidative stress and its mechanisms of resistance to rapeseed. Journal of Plant Researches. 21(3): 421-432. (In Persian).
Mishra, S. and Dubey, R.S. (2006). Heavy metal uptake and detoxification mechanisms in plants. International Journal of Agricultural Research. 1(2): 122-141.
Mittler, R., Vanderauwera, S., Gollery, M. and Breusegem, F.V. (2004). The reactive oxygen gene network of plants. Trends Plant Science. 9: 490-498.
Moon, J.H. and Terao, J. (1998). Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agricultural and Food Chemistry. 46(12): 5062-5065.
Morgan, J.M. (1988). The use of coleoptile responses to osmoregulation; growth and yield. Annals of Botany. 62: 193-8.
Naderi, N., Mirzamasoumzadeh, B. and Aghaei, A. (2013). Effects of different levels of Lead (Pb) on physiological characteristics of sugar beet. International Journal of Agriculture and Crop Sciences. 5(10): 1154-1157.
Neill, S., Boini, M.K., Lodagala, S.D. and Behara, R.B.( 2007). The fresh leaves of Catharantus roseus Linn Reduces blood glucose in normal and alloxan diabetric rabbites. BMC Complement Altern Med, 3(4):433-440.
Omidbaigi, R. (2004). Production and processing of medicinal plants. Astan Qods Razavi Publisher. [In Persian].
Pallavi, Sh. and Rama, Sh.D. (2005). Lead toxicity in plant. Brazilian Journal of Plant Physiology. 17: 1-6.
Patra, M., Bhowmik, N., Bandopadhyay, B. and Sharma, A. (2004). Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environmental and Experimental Botany. 52(3):199-223.
Pichtel, J. and Carol, A.S. (1998). Vegetative growth and trace metal accumulation on metalliferous wastes. Journal of Environmental Quality. 27: 618-24.
Popova, L., Ananieva, V., Hristova, V., Christov K., Georgieva, K., Alexieva, V. and Stoinova Zh. (2003). Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology. 2003: 133-152.
Pourrut, B., Shahid, M., Dumat, C., Winterton, P. and Pinelli E. (2012). Lead uptake, toxicity, and detoxification in plants. Reviews of Environmental Contamination and Toxicology. 213:113-36.
Prakash, V. (1990). Leafy Spices. CRC Press, 114 p.
Rajabi, A., Abaspour, H. and Sinaki, J.M. (2016). The effect of chemical stimulants of methyl jasmonate and salicylic acid on stimulating the production of Hypersin in Hypericum perforatum. New Cellular and Molecular Biotechnology Journal. 6(22):41-49. (In Persian).
Ranjbar, M., Lari Yazdi, H. and Bromandjazi, Sh. (2011). Effect of salicylic acid on photosynthetic pigments, sugar content and antioxidant enzymes in rapeseed under lead stress. Journal of Plant Researches. 3(9):39-52. (In Persian).
Rao, M.S.S. and Mendham, N.J. (1991). Soil–plant–water relations of oilseed rape (Brassica napus and B. campestris). The Journal of Agricultural Science. 117(02): 197-205.
Resh, H.M. (2001). Hydroponic food production. Woodbridge press publishing. Company, Santa Barbara.
Rudell, D.R. and Matteis, J.P. (2002). Methyl Jasmonate enhances antocyanin accumulation and modifies production of phenolics and pigments in Fuji apples. Horticultural Science. 127: 435-41.
Sadasivam, S. and Manickam, A. (1992). Biochemical methods for agricultural sciences. Wiley Eastern Limited.
Samadi, S., Ghasemnezhad, A. and Alizadeh, M. (2014). Investigation on phenylalanine ammonia-lyase activity of artichoke (Cynara scolymus L.) affected by methyl jasmonate and salicylic acid in in-vitro conditions. Plant Products Research Journal. 21(4): 135-48.
Samardakiew, S. and Wozny, A. (2000). Thedistribution of lead in dunckweed root tip. Plant and Soil. 226: 107-111.
Sánchez, F.J., Manzanares, M., de Andres, E.F., Tenorio, J.L. and Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research. 59(3): 225-235.
Sebastiani, L., Scebba, F. and Tognetti, R. (2004). Heavy metal accumulation and growth responses in poplar clones Eridano (Populus deltoides × maximowiczii) and I 214 (P. × euramericana) exposed to industrial waste. Environmental and Experimental Botany. 52:79–88.
Senaranta, T., Touchell, D., Bum M.E. and Dixon, K. (2002). Acetylsalicylic (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation. 30:157-161.
Sengar, R., Gautam, M., Sengar, R., Sengar, R., Garg, S., Sengar, K. and Chaudhary, R. (2008). Lead stress effects on physiobiochemical activities of higher plants. Reviews of Environmental Contamination and Toxicology Vol. 196. D.M. Whitacre, Springer US. 196:73-93.
Shakirova, F.M., Sakhabutdinova, A.R., Bozrutkova, M.V., Fatkhutdinova, R.A. and Fatkhutdinova, D.R. (2003). Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science. 164: 317-322.
Sharma, P. and Dubey, R. S. (2004). Ascorbateperoxides from rice seedling. Plant Science. 167: 541-550.
Sharma, A., Jha, A.M., Dubey, R.S. and Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plant under stressful conditions. Journal of Botany. 26: 1–26.
Singh, G.R.K.A., Reshma, R.K. and Ahmad, M. (2012). Effect of lead and nickel toxicity on chlorophyll and proline content of Urd (Vigna mungo L.) seedlings. International Plant Physiology and Biochemistry. 4(6): 136-41.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16(3):144-158.
Szabados, L. and Savouré, A. (2010). Proline: a multifunctional amino acid. Trends in Plant Science. 15:89-97.
Sonar, B.A., Nivas, M.D., Gaikwad, D.K., and Chavan, P.D.( 2011). Assessment of salinity-induced antioxidative defense system in Colubrina asiatica brong. Journal of Stress Physiology and Biochemistry, 7: 193–200.
Tan, J., Zhao, H., Hoang, J., Han, Y., Li, H. and Zhao, W. (2008). Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat Seedlings subjected to osmotic stress. World Journal of Agricultural Science. 4:307-13.
Tsuchiya, T., Ohta, H., Okawa, K., Iwamatsu, A., Shimada, H., Masuda, T. and Takamiya, K. (1999). Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate. Proceeding of The National Academy of Sciences of the United States of America. 96: 15362–15367.
Vatan Khah, A., Kalantari, B. and Andalibi, B. (2015). Methyl-jasmonate effect on some physiological and biochemical responses of peppermint (Mentha piperita L.) under salinity stress. Journal of Plant Process and Function. 5(17): 157-170. (In Persian).
Verma, S. and Dubey, R.S.(2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant in growing rice plants. Plant Science, 164: 1489– 1498.
Walia, H., Wilson, C., Condamine, P., Liu, X., Ismoil, A.M. and Close, T.J. (2007). Large–scale expression profiling and physiological characterization of jasmonic acid– mediated adaptation of barley to salinity stress. Plant Cell and Environment. 30:410-421.
Wang, K., Jin, P., Cao, S., Shang, H., Yang, Z. and Zheng, Y. (2009). Methyl jasmonate reduces decay and enhances antioxidant capacity in chines bay berries. Journal of Agricultural and Food Chemistry. 57: 5809-5850.
Wang, S.Y. (1999). Methyl Jasmonate reduces water stress in strawberry. Plant Growth Regulation. 18:127-134.
Wasternack, C. and Kombrink, E. (2009). Jasmonates structural requirements for lipid-derived signals active in plant stress responses and development. ACS Chemical Biology. 5:63-77.
Wasternack, C. and Parthier, B. (1997). Jasmonate signalled plant gene expression. Trends in Plant Science. 2: 302–307.
Weidhase, R.A., Kramell, H., Lehmann, J., Liebisch, H., Lerbs, W. and Parthier, B. (1987). Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Science. 51:177-186.
Yaron, B., Calvet, R. and Prost, R. (1996). Soil pollution: processes and dynamics. Springer-Verlag, Berlin Heidelberg, 312 p.
Zawoznik, M.S., Gropp, M.D., Tomaro, M.L. and Benavides, M.P. (2007). Endogenoussalicylic acid potentiates cadmium- inducedoxidative stress in Arabidopsis thaliana. Plant Science. 173:190-19.
Zhang, W., Curtin, C., Kikuchi, M. and Franco, C. (2002). Integration of jasmonic acid and lightirradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Science. 162: 459–468.
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Abdala, G., Miersch, O., Kramell, R., Vigliocco, A., Agostini, E., Forchetti, G. and Alemano, S. (2003). Jasmonate and octadecanoid occurrence in tomato hairy roots. Endogenous level changes in response to NaCl. Plant Growth Regulation. 40: 21-27.
Al-Amier, H. and Craker, L. E. (2007). In-vitro selection for stress tolerant spearmint. Issues in New Crops and New Uses, pp. 306-310.
Aldoobie, N.F. and Beltagi, M.S. (2013). Physiological, biochemical and molecular responses of common bean (Phaseolus vulgaris L.) plants to heavy metals stress. African Journal of Biotechnology. 12(29): 4614-4622.
Amirjani, M., Abnosi, M.H., Mahdieh, M. and Ghareshekhlou, S. (2015). Study of the effect of lead on the activity of antioxidant enzymes, proline and total alkaloids of callus of Catharantus roseus. Cell and Tissue Journal. 6(1): 9-21. (In Persian).
Arabaci, D. and Bayram, E. (2004). The effect of nitrogen fertilization and different plant densities on some agronomic and technologic characteristic of Ocimum basilicum L. (Basil). Journal of Plant Biology Research. 3(4): 255-62.
Arora, A., Byrem, T.M., Nair, M. and Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics. 373:102–109.
Asadi Karam, A., Asrar, Z. and Keramat, B. (2013). Effect of methyl-jasmonate treatment on the content of phenolic compounds and PAL activity in Lepidium sativum under the toxicity of copper. Journal of Plant Process and Function. 2(6): 89-96. (In Persian).
Ayala-Zavala J.F., Wang, S.Y., Wang, C.Y. and Gonzalez-Aguilar, G.A. (2005). Methyl jasmonate in conjunction with ethanol treatment increases antioxidant capacity, volatile compounds and post-harvest life of straw berry fruit. European Food Research and Technology. 221: 731-8.
Babst, B.A., Ferrieri, R.A., Gray, D.W., Lerdau, M., Schyler, D.J., Schueller, M., Thorpe, M.R. and Orians, C.M. (2005). Jasmonic acid induces rapid changes in carbon transport and partitioning in populus. New Phytologist. 167: 63-72.
Baker, A.J.M., Mc Grath, S.P., Reeves, R.D. and Smith, J.A.C. (2000) . Metal hyperaccumulator plants: a review of the ecology and physiology of a biological resource for phytoremediation of metal-polluted soils. In: Phytoremediation of contaminated soil and water (eds. Terry, N. and Banuelos, G.). CRC Press, Boca Raton, Florida, USA. 85-107.
Barrientos Carvacho, H., Pérez, C., Zúñiga, G. and Mahn, A. (2014). Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts. Journal of the Science Food and Agriculture. 63(1): 20-31.
Bates, L.S., Waldren, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water-stress studies. Plant and Soil. 39(1): 205-207.
Beladi, S.M., Kashani, A., Habibi, D., Paknejad, F. and Golshan, M. (2010). The effect of lead and copper on some physiological traits of Lathyrus sativus L. New Finding in Agriculture. 5(1): 9-20. (In Persian).
Brunet, J., Repellin, A., Varrault, G., Terrync, N. and Zuily-fodil, Y. (2008). Lead accumulation in the roots of grass pea (Lathyrus sativus): a novel plant for phytoremediation systems. Comptes Rendus Biologies. 331: 859-864.
Bruni, R. and Sacchetti, G. (2009). Factors affecting polyphenol biosynthesis in wild and field grown St. John´s Wort (Hypericum perforatum L. Hypericaceae/ Guttiferael). Molecules. 14: 682-725.
Capitani, F., Biondi, S., Falasca, G., Ziosi, V., Balestrazzi, A., Carbonera, D., Torrigiani, P. and Altamura, M. (2005). Methyl jasmonate disrupts shoot formation in tobacco thin cell layers by over-inducing mitotic activity and cell expansion. Planta. 220: 507-519.
Chen, J., Zhu, C., Lin, D. and Sun, Z. X. (2007). The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings. Canadian Journal of Plant Science. 87: 49-57.
Chien, H.F., Wang, J.W., Lin, C.C. and Kao C.H. (2001). Cadmium toxicity of rice leaves is mediated through lipid peroxidation. Journal of Plant Growth Regulation. 33: 205-213.
Choudhury, S. and Panda, S.K. (2004). Role of salicylic acid in regulating cadmium induced oxidative stress in Oryza sativa L roots. Bulgarian Journal of Plant Physiology. 30: 95-110.
Comparot, S.M., Graham, C.M. and Reid, D.M. (2002). Methyl jasmonate elicits a differential antioxidant response in light and dark grown canola (Brassica napus) roots and shoots. Journal Plant Growth Regulation. 38: 21-30.
Creelman, R. A. and Mullet, J. E. (1997). Oligosacchrins, brassinolides and jasmonates: non traditional regulators of plant growth, development, and gene expression. American Society of Plant Biology. 9: 1211- 1223.
Divya, P., Puthusseri, B. and Neelwarne, B. (2013). The effect of plant regulators on the concentration of caretonoids and phenolic compound in foliage o coriander. LWT-Food Science and Technology. 56(1):101-110.
Dong, J., Wan, G. and Liang, Z. (2010). Accumulation of salicylic acid induced phenolic compounds and raised activities of secondary metabolic and antioxidant enzymes in Salvia miltorrhiza cell culture. Journal of Biotechnology. 148: 99-104.
Garcia-Mata, C. and Lamattina, L. (2001). Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiology. 126(3): 1196-1204.
Ghelich, S., Zarin Kamar, F. and Niknam, V. (2015). Investigating the amount of lead accumulation and its effect on peroxidase activity, phenolic and flavonoids content in germination stage in alfalfa (Medicago sativa L.). Journal of Plant Researches. 28(1): 164-174. (In Persian).
Gonzalez-Aguilar, G., Tiznado-Hernandez, M.E. and Zavaleta-Gaticar Martinez-Tellez, M.A. (2004). Methyl jasmonat treatment reduce chilling injury and activate the defense of guava fruits. Biochemical and Biophysical Research Communications. 313: 694-701.
Hashemi, Sh., Asrar, Z. and Pourseyidi, Sh. (2010). Effect of seed premixing by salicylic acid on growth and some physiological and biochemical indices in cress. Journal of Plant Researches. 2(2):1-10. (In Persian).
Heredia, J.B. and Cisneros-Zevallos, L. (2009). The effect of exogenous ethylene and methyl jasmonate on PAL activity, phenolic profiles and antioxidant capacity of carrots (Daucus carota) under different wounding intensities. Postharvest Biology and Technology, 51(2): 242-249.
Horton, R.F. (1991). Methyl Jasmonate and transpiration in barley. Plant Physiology. 96:1376-1378.
Islam, E., Yang, X., Li T., Liu D., Jin, X. and Meng, F. (2007). Effect of Pb toxicity on root morphology, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. Journal of Hazardous Materials. 147(3): 806–16.
John, R., Ahmad, P., Gadgil, K. and Sharma, S. (2009). Heavy metal toxicity: Effect on plant growth, biochemical parameters and metal accumulation by Brassica juncea L. International Journal of Plant Diseases and Protection. 3(3): 1735-8043.
Jung, S. (2004). Effect of chlorophyll reduction in Arabidopsis thaliana by methyl jasmonate or norflurazon on antioxidant systems. Journal of Plant Physiology and Biochemistry. 42: 231-255.
Kang, H.M. and Saltveit, M.E. (2002). Antioxidant enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles. Journal of Agricultural and Food Chemistry. 50(3): 513-518.
Kim, Y. Y., Yang, Y., and Lee, Y. (2002). Pb andCd uptake in rice roots. Plant Physiology. 116: 368-372.
Kovacik, J., Klejdus, B., Hedbavny, J., Covska, S. and Zon, J. (2010). Significance of phenols in cadmium and nickel uptake. Journal of Plant Physiology. 168: 576-584.
Kumar, A. and Elston, J. (1992). Genotypic differences in leaf water relations between Brassica juncea and B. napus. Annals of Botany. 70(1): 3-9.
Kumari, G.J., Reddy, A.M., Naik, S.T., Kumar, S.G., Prasanthi, J., Sriranganayakulu, G., Reddy, P.C. and Sudhakar, C. (2006). Jasmonic acid induced changes in protein pattern, antioxidative enzyme activities and peroxidase isozymes in peanut seedlings. Biologia Plantarum. 50: 119-226.
Liamas, A., Ullrich, C.I. and Sanz, A. (2000). Cadmium effects on transmembrane electrical potential difference, respiration and membrane permeability of rice (Oryza sativa) roots. Plant and Soil. 219: 21-8.
Lutts, S., Kinet, J.M. and Bouharmont, J. (1995). Changes in plant response to NaCl during development of rice (Oryza sativa L.) varieties differing in salinity resistance. Journal of Experimental Botany. 46(12): 1843-1852.
Lutts, S., Kinet, J.M. and Bouharmont, J. (1996). NaCl-induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Annals of Botany. 78(3): 389-398.
Maciejewska, W. and Krupa, Z. (2002). Jasmonic acid and heavy metals in Arabidopsis plant a similar physiological response to both stressors. Plant Physiology. 159: 509-515.
Maksymiec, W. and Krupa, Z. (2006). The effects of shortterm exposition to Cd, excess Cu ions and jasmonate on oxidative stress appearing in Arabidopsis thaliana. Environmental and Experimental Botany. 57: 187-194.
Mazaheri Tirani, M., Kalantari, Kh. and Hasibi, N. (2008). Study of the interaction of ethylene and salicylic acid on oxidative stress and its mechanisms of resistance to rapeseed. Journal of Plant Researches. 21(3): 421-432. (In Persian).
Mishra, S. and Dubey, R.S. (2006). Heavy metal uptake and detoxification mechanisms in plants. International Journal of Agricultural Research. 1(2): 122-141.
Mittler, R., Vanderauwera, S., Gollery, M. and Breusegem, F.V. (2004). The reactive oxygen gene network of plants. Trends Plant Science. 9: 490-498.
Moon, J.H. and Terao, J. (1998). Antioxidant activity of caffeic acid and dihydrocaffeic acid in lard and human low-density lipoprotein. Journal of Agricultural and Food Chemistry. 46(12): 5062-5065.
Morgan, J.M. (1988). The use of coleoptile responses to osmoregulation; growth and yield. Annals of Botany. 62: 193-8.
Naderi, N., Mirzamasoumzadeh, B. and Aghaei, A. (2013). Effects of different levels of Lead (Pb) on physiological characteristics of sugar beet. International Journal of Agriculture and Crop Sciences. 5(10): 1154-1157.
Neill, S., Boini, M.K., Lodagala, S.D. and Behara, R.B.( 2007). The fresh leaves of Catharantus roseus Linn Reduces blood glucose in normal and alloxan diabetric rabbites. BMC Complement Altern Med, 3(4):433-440.
Omidbaigi, R. (2004). Production and processing of medicinal plants. Astan Qods Razavi Publisher. [In Persian].
Pallavi, Sh. and Rama, Sh.D. (2005). Lead toxicity in plant. Brazilian Journal of Plant Physiology. 17: 1-6.
Patra, M., Bhowmik, N., Bandopadhyay, B. and Sharma, A. (2004). Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance. Environmental and Experimental Botany. 52(3):199-223.
Pichtel, J. and Carol, A.S. (1998). Vegetative growth and trace metal accumulation on metalliferous wastes. Journal of Environmental Quality. 27: 618-24.
Popova, L., Ananieva, V., Hristova, V., Christov K., Georgieva, K., Alexieva, V. and Stoinova Zh. (2003). Salicylic acid and methyl jasmonate induced protection on photosynthesis to paraquat oxidative stress. Bulgarian Journal of Plant Physiology. 2003: 133-152.
Pourrut, B., Shahid, M., Dumat, C., Winterton, P. and Pinelli E. (2012). Lead uptake, toxicity, and detoxification in plants. Reviews of Environmental Contamination and Toxicology. 213:113-36.
Prakash, V. (1990). Leafy Spices. CRC Press, 114 p.
Rajabi, A., Abaspour, H. and Sinaki, J.M. (2016). The effect of chemical stimulants of methyl jasmonate and salicylic acid on stimulating the production of Hypersin in Hypericum perforatum. New Cellular and Molecular Biotechnology Journal. 6(22):41-49. (In Persian).
Ranjbar, M., Lari Yazdi, H. and Bromandjazi, Sh. (2011). Effect of salicylic acid on photosynthetic pigments, sugar content and antioxidant enzymes in rapeseed under lead stress. Journal of Plant Researches. 3(9):39-52. (In Persian).
Rao, M.S.S. and Mendham, N.J. (1991). Soil–plant–water relations of oilseed rape (Brassica napus and B. campestris). The Journal of Agricultural Science. 117(02): 197-205.
Resh, H.M. (2001). Hydroponic food production. Woodbridge press publishing. Company, Santa Barbara.
Rudell, D.R. and Matteis, J.P. (2002). Methyl Jasmonate enhances antocyanin accumulation and modifies production of phenolics and pigments in Fuji apples. Horticultural Science. 127: 435-41.
Sadasivam, S. and Manickam, A. (1992). Biochemical methods for agricultural sciences. Wiley Eastern Limited.
Samadi, S., Ghasemnezhad, A. and Alizadeh, M. (2014). Investigation on phenylalanine ammonia-lyase activity of artichoke (Cynara scolymus L.) affected by methyl jasmonate and salicylic acid in in-vitro conditions. Plant Products Research Journal. 21(4): 135-48.
Samardakiew, S. and Wozny, A. (2000). Thedistribution of lead in dunckweed root tip. Plant and Soil. 226: 107-111.
Sánchez, F.J., Manzanares, M., de Andres, E.F., Tenorio, J.L. and Ayerbe, L. (1998). Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. Field Crops Research. 59(3): 225-235.
Sebastiani, L., Scebba, F. and Tognetti, R. (2004). Heavy metal accumulation and growth responses in poplar clones Eridano (Populus deltoides × maximowiczii) and I 214 (P. × euramericana) exposed to industrial waste. Environmental and Experimental Botany. 52:79–88.
Senaranta, T., Touchell, D., Bum M.E. and Dixon, K. (2002). Acetylsalicylic (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulation. 30:157-161.
Sengar, R., Gautam, M., Sengar, R., Sengar, R., Garg, S., Sengar, K. and Chaudhary, R. (2008). Lead stress effects on physiobiochemical activities of higher plants. Reviews of Environmental Contamination and Toxicology Vol. 196. D.M. Whitacre, Springer US. 196:73-93.
Shakirova, F.M., Sakhabutdinova, A.R., Bozrutkova, M.V., Fatkhutdinova, R.A. and Fatkhutdinova, D.R. (2003). Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science. 164: 317-322.
Sharma, P. and Dubey, R. S. (2004). Ascorbateperoxides from rice seedling. Plant Science. 167: 541-550.
Sharma, A., Jha, A.M., Dubey, R.S. and Pessarakli, M. (2012). Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plant under stressful conditions. Journal of Botany. 26: 1–26.
Singh, G.R.K.A., Reshma, R.K. and Ahmad, M. (2012). Effect of lead and nickel toxicity on chlorophyll and proline content of Urd (Vigna mungo L.) seedlings. International Plant Physiology and Biochemistry. 4(6): 136-41.
Singleton, V.L. and Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture. 16(3):144-158.
Szabados, L. and Savouré, A. (2010). Proline: a multifunctional amino acid. Trends in Plant Science. 15:89-97.
Sonar, B.A., Nivas, M.D., Gaikwad, D.K., and Chavan, P.D.( 2011). Assessment of salinity-induced antioxidative defense system in Colubrina asiatica brong. Journal of Stress Physiology and Biochemistry, 7: 193–200.
Tan, J., Zhao, H., Hoang, J., Han, Y., Li, H. and Zhao, W. (2008). Effects of exogenous nitric oxide on photosynthesis, antioxidant capacity and proline accumulation in wheat Seedlings subjected to osmotic stress. World Journal of Agricultural Science. 4:307-13.
Tsuchiya, T., Ohta, H., Okawa, K., Iwamatsu, A., Shimada, H., Masuda, T. and Takamiya, K. (1999). Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: finding of a lipase motif and the induction by methyl jasmonate. Proceeding of The National Academy of Sciences of the United States of America. 96: 15362–15367.
Vatan Khah, A., Kalantari, B. and Andalibi, B. (2015). Methyl-jasmonate effect on some physiological and biochemical responses of peppermint (Mentha piperita L.) under salinity stress. Journal of Plant Process and Function. 5(17): 157-170. (In Persian).
Verma, S. and Dubey, R.S.(2003). Lead toxicity induces lipid peroxidation and alters the activities of antioxidant in growing rice plants. Plant Science, 164: 1489– 1498.
Walia, H., Wilson, C., Condamine, P., Liu, X., Ismoil, A.M. and Close, T.J. (2007). Large–scale expression profiling and physiological characterization of jasmonic acid– mediated adaptation of barley to salinity stress. Plant Cell and Environment. 30:410-421.
Wang, K., Jin, P., Cao, S., Shang, H., Yang, Z. and Zheng, Y. (2009). Methyl jasmonate reduces decay and enhances antioxidant capacity in chines bay berries. Journal of Agricultural and Food Chemistry. 57: 5809-5850.
Wang, S.Y. (1999). Methyl Jasmonate reduces water stress in strawberry. Plant Growth Regulation. 18:127-134.
Wasternack, C. and Kombrink, E. (2009). Jasmonates structural requirements for lipid-derived signals active in plant stress responses and development. ACS Chemical Biology. 5:63-77.
Wasternack, C. and Parthier, B. (1997). Jasmonate signalled plant gene expression. Trends in Plant Science. 2: 302–307.
Weidhase, R.A., Kramell, H., Lehmann, J., Liebisch, H., Lerbs, W. and Parthier, B. (1987). Methyljasmonate-induced changes in the polypeptide pattern of senescing barley leaf segments. Plant Science. 51:177-186.
Yaron, B., Calvet, R. and Prost, R. (1996). Soil pollution: processes and dynamics. Springer-Verlag, Berlin Heidelberg, 312 p.
Zawoznik, M.S., Gropp, M.D., Tomaro, M.L. and Benavides, M.P. (2007). Endogenoussalicylic acid potentiates cadmium- inducedoxidative stress in Arabidopsis thaliana. Plant Science. 173:190-19.
Zhang, W., Curtin, C., Kikuchi, M. and Franco, C. (2002). Integration of jasmonic acid and lightirradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Science. 162: 459–468.
