ارزیابی تجربی تاثیر اسید فولیک بر ترمیم زخم سوختگی قرنیه در خرگوش سفید نیوزلندی
الموضوعات :
امیرحسین محلوجیان
1
,
علیرضا جهاندیده
2
,
احمد اصغری
3
,
سید پژمان مرتضوی
4
1 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده علوم تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - دانشیار گروه جراحی، دانشکده علوم تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - دانشیار گروه جراحی، دانشکده علوم تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
4 - دانشیار گروه پاتوبیولوژی، دانشکده علوم تخصصی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
تاريخ الإرسال : 01 الإثنين , جمادى الأولى, 1440
تاريخ التأكيد : 12 السبت , ربيع الأول, 1441
تاريخ الإصدار : 26 الثلاثاء , جمادى الأولى, 1441
الکلمات المفتاحية:
اسید فولیک,
خرگوش,
زخم قرنیه,
آنتی اکسیدان‌ها,
ملخص المقالة :
زخم قرنیه یکی از بیماریهای به نسبت شایع چشم است که در اثر ضربه، مواد شیمیایی و دخالت برخی عوامل ویروسی، باکتریایی و کلامیدیایی ممکن است رخ دهد. این مطالعه به منظور بررسی اثر اسید فولیک بر ترمیم زخم سوختگی قرینه روی 20 سر خرگوش سفید نیوزلندی نر بالغ در یک محدوده وزنی و سنی انجام شد.بدین منظور،بعد از بیهوشی کامل، زخم قرینه در چشم چپ هر یک از خرگوشها ایجاد شد و پس از ایجاد سوختگیبرای اطمینان از یکسان بودن زخم‌‌‌‌‌ها (قطر 6 میلی‌متر) رنگ آمیزی چشم با فلورسئین انجام شد. سپس خرگوشها به چهار گروه مساوی شامل سه گروه آزمایشی و یک گروه شاهد تقسیم شدند. در گروه های آزمایشی (اول تا سوم) اسید فولیک به ترتیب به مقدار 2، 5 و 10 میلی‌گرم به ازای هر کیلوگرم وزن بدن، روزانه و تا 21 روز بعد از جراحی گاواژ شد. در آسیب‌شناسی بافتی عروق زایی، میزان التهاب و ادم بستره قرینه در خرگوش‌‌‌‌‌‌ها‌ی تحت درمان با اسید فولیک در مقایسه با گروه شاهد به طور معنیداری کاهش نشان داد، اما لایه‌ها‌ی بافت پوششی قرینه افزایش یافته بود (05/0>p ). همچنین استفاده از اسید فولیک به طور معنیداری موجب بهبود نظم طبیعی رشته‌‌‌‌‌‌‌‌ها‌ی کلاژن قرینه شد (05/0>p ). براساس نتایج بدست آمده به نظر می‌‌‌‌‌‌‌‌‌‌‌‌رسد که اسید فولیکاثرات مناسب و قابل قبولی بر روند ترمیم زخم قرینه خرگوش داشته باشد.
المصادر:
Alio, J.L., Ayala, M.J., Mulet, M.E., Artola, A., Ruiz, J.M. and Bellot, J. (1995). Antioxidant Therapy in the Treatment of Experimental Acute Corneal Inflammation. Karger Medical and Scientific Publishers, 2(7): 136-143.
Babior, B.M. and Bunn, H.F. (1994). Megaloblastic anemias. In: Harrison's Principles of Internal Medicine. Isselbacher, K.J., Braunwald, E., Wilson, J.O., Martin, J.B., Fauci, A.S. and Kasper, D.L. editors. 13th ed., New York: McGraw-Hill, pp: 1726-1732.
Bewndich, A. and Dekelbaum, R.J. (1997). Preventive Notrition, The comprehensive guid for helth Professionals. New Jersey Torowa Press, pp: 351-371.
Boushey, C., Beresford, A.A., Omenn, G.S. and Motulsky, A.A. (1996). Quantitative assessment of plasma homocysteine as a risk factor for vascular diseases. Journal of Nutrigenetics and Nutrigenomics 27(4): 1049-1057.
Burrner, F.N. (1991). Use of fulic acid for prevention of spina bifida and other neural rube defects. Journal of the American Medical, 26(6): 1191-1197.
De Silva, P., Jayammanne, G. and Bolton, R. (2008). Folic acid deficiency optic neuropathy. Journal of Medical Case Reports, 7(3): 1-3.
Farhoodi, M., Majidi, J., Talebi, M., Yazdchi, M., Alizade, M. and Arjangnia, F. (2008). Evaluation of serum homocysteine level in Parkinson's disease or its association with severity of disease. Journal of Tabriz University of Medical Sciences, 29(4): 47-51. [In Persian]
Fellstrom, B., Akuyrek, L.M. and Zezina, L. (1998). Post ischemic reperfusion injury and allograft arteriosclerosis. Transplantation Proceedings, 3(8): 4278-4280.
Giles, W.H., Kittner, S., Anda, R.F., Croft, B. and Casper, M.L. (1995). Serum folate and risk for ischemic stroke first national health and nutrition examination survey epidemiologic follow-up study. Stroke, 26(11): 66-70.
Girod, A. (1970). The Nutrition of the Embroyo. 2nd ed., Illionoise, Springfield, pp: 690-701.
Kasetsuwan, N., Wu, F.M., Hsieh, F., Sanchez, D. and Mc-Donnell, P.J. (1999). Effect of topical ascorbic acid on free radical tissue damage and inflammatory cell influx in the cornea after excimer laser corneal surgery. Archives of Ophthalmology, 117(5): 649-652.
Lalonde, R., Barraud, H., Ravey, J., Gueant, J.L., Bronowicki, J.P. and Strazielle, C. (2008). Effects of a B-vitamin-deficient diet on exploratory activity, motor coordination and spatial learning in young adult Balb/c mice. Annals of the New York Academy of Sciences, 118(8): 122-131.
Lalonde, R., Joyal, C.C. and Botez, M.I. (1993). Effects of folic acid and folinic acid on cognitive and motor behaviors in 20-month-old rats. Journal of Pharmacology, Biochemistry, and Behavior, 44(3): 703-707.
Lewis, C.A., Pancharuniti, N. and Sauberlich, H.E. (1992). Plasma folate adequacy as determined by homocysteine level. Annals of the New York Academy of Sciences, 66(9): 360-368.
Li Y., Feng, G., Yi Y. and Lin J. (1999). The experimental investigation of epithelial healing in rabbit central corneal alkali wounds. Annals of Eye Science, 15(2): 74-77.
Liu, R. and Pravia, K. (2010). Oxidative stress and glutathione in TGF-β-mediated fibrogenesis. Radical Biology and Medicine, 48(1): 1-15.
Maher, J.J. (2001). Interactions between hepatic stellate cells and the immune system. Seminars in Liver Disease, 21(3): 417-426.
Mannino, D.M., Mulinare, J., Ford, E.S. and Schwartz, J. (2003). Tobacco smoke exposure and decreased serum and red blood cell folate levels: data from the third national health and nutrition examination Survey. Nicotine and Tobacco Research, 5(3): 357-362.
Marsillach, J., Ferre, N., Camps, J., Riu, F., Rull, A. and Joven, J. (2008). Moderately high folic acid supplementation exacerbates experimentally induced liver fibrosis in rats. Radical Biology and Medicine, 233(1): 38-47.
Meller, D., Pires, R.T., Mack, R.G., Figueiredo, F., Heiligenhaus, A., Park, W.C., et al. (2000). Amniotic membrane transplantation for acute chemical or thermal burns. Ophthalmology, 107(5): 980-989.
Miller, A.L. (2003). The methionin-homocystein cycle and its effects on cognitive diseases. Scientific Review of Alternative Medicine, 63(8): 7-19.
Mills, J., Rhoads, G. and Simpson, J. (1989). The absence of a relationbetween the periconceptional use of vitamins and neural tube defects. The New England Journal of Medicine, 32(1): 430-435.
Nicholls, P. (1975). Formate as an inhibitor of cytochrome-C oxidase. Biochemical and Biophysical Research Communications, 67(2): 610-616.
Pietrzik, K., Bailey, L. and Shane, B. (2010). Folic acid and L-5-methyltetrahydrofolate. Clinical Pharmacokinetics, 49(8): 535-548.
Ponziani, F.R., Cazzato, I., Danese, S., Fagiuoli, S., Gionchetti, P., Annicchiarico, B., et al. (2012). Folate in gastrointestinal health and disease. European Review for Medical and Pharmacological Sciences, 16(3): 376-385.
Rinastiti, M., Harijadi, A., Santoso, A.L. and Sosroseno, W. (2006). Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. International Journal of Oral and Maxillofacial Surgery, 35(3): 247-51.
Selhub, J., Jacques, P.F., Wilson, P.W., Rush, D. and Rosenberg, I.H. (1993). Vitamin status and intake as primary determinants of homocysteinemia in an elderly population. Journal of the American Medical, 230(70): 269-438.
Seth, P., Kumari, R., Madhavan, S., Singh, A.K., Mani, H., Banaudha, K., et al. (2000). Prevention of renal ischemia reperfusion-induced injury in rats by picroliv. Biochemical Pharmacology, 59(10): 1315-1322.
Shimazaki, J., Yang, H.Y. and Tsubota, K. (1997). Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burns. Ophthalmology, 104(12): 2068-2076.
Shoham, A., Hadziahmetovic, M., Dunaief, J.L., Mydlarski, M.B. and Schipper, H.M. (2008). Oxidative stress in diseases of the human cornea. Radical Biology and Medicine, 45(8): 1047-1055.
Slatter, D. (2003). Eye and adnexa. In: Text book of Small Animal Surgery. Basher, T. editor. 2rd ed., Saunders, pp: 1354-1486.
Strubbe, D.T., Brooks, D.E., Schultz, G.S., Willis‐Goulet, H., Gelatt, K.N., Andrew, S.E., et al. (2000). Evaluation of tear film proteinases in horses with ulcerative keratitis. Veterinary Ophthalmology, 3(2-3): 111-119.
Unal, D., Yeni, E., Erel, O., Bitiren, M. and Vural, H. (2002). Antioxidative effects of exogenous nitric oxide versus antioxidant vitamins on renal ischemia reperfusion injury. Undergraduate Research Opportunities, 30(3): 190-194.
Walter, M.M., Shapior, S. and Mitchell, A.A. (1993). Periconceptional folic acidexposure and risk of oceurent tube defects. Journal of the American Medical, 269(10): 1257-1261.
Xiao-Wei, G., Xu-Dong, Z., Wen-Jing, L., Xin, Z. and Ying, L. (2012). Experimental study on the treatment of rabbit corneal melting after alkali burn with Collagen cross-linking. International Journal of Ophthalmology, 5(2): 147-150.
_||_
Alio, J.L., Ayala, M.J., Mulet, M.E., Artola, A., Ruiz, J.M. and Bellot, J. (1995). Antioxidant Therapy in the Treatment of Experimental Acute Corneal Inflammation. Karger Medical and Scientific Publishers, 2(7): 136-143.
Babior, B.M. and Bunn, H.F. (1994). Megaloblastic anemias. In: Harrison's Principles of Internal Medicine. Isselbacher, K.J., Braunwald, E., Wilson, J.O., Martin, J.B., Fauci, A.S. and Kasper, D.L. editors. 13th ed., New York: McGraw-Hill, pp: 1726-1732.
Bewndich, A. and Dekelbaum, R.J. (1997). Preventive Notrition, The comprehensive guid for helth Professionals. New Jersey Torowa Press, pp: 351-371.
Boushey, C., Beresford, A.A., Omenn, G.S. and Motulsky, A.A. (1996). Quantitative assessment of plasma homocysteine as a risk factor for vascular diseases. Journal of Nutrigenetics and Nutrigenomics 27(4): 1049-1057.
Burrner, F.N. (1991). Use of fulic acid for prevention of spina bifida and other neural rube defects. Journal of the American Medical, 26(6): 1191-1197.
De Silva, P., Jayammanne, G. and Bolton, R. (2008). Folic acid deficiency optic neuropathy. Journal of Medical Case Reports, 7(3): 1-3.
Farhoodi, M., Majidi, J., Talebi, M., Yazdchi, M., Alizade, M. and Arjangnia, F. (2008). Evaluation of serum homocysteine level in Parkinson's disease or its association with severity of disease. Journal of Tabriz University of Medical Sciences, 29(4): 47-51. [In Persian]
Fellstrom, B., Akuyrek, L.M. and Zezina, L. (1998). Post ischemic reperfusion injury and allograft arteriosclerosis. Transplantation Proceedings, 3(8): 4278-4280.
Giles, W.H., Kittner, S., Anda, R.F., Croft, B. and Casper, M.L. (1995). Serum folate and risk for ischemic stroke first national health and nutrition examination survey epidemiologic follow-up study. Stroke, 26(11): 66-70.
Girod, A. (1970). The Nutrition of the Embroyo. 2nd ed., Illionoise, Springfield, pp: 690-701.
Kasetsuwan, N., Wu, F.M., Hsieh, F., Sanchez, D. and Mc-Donnell, P.J. (1999). Effect of topical ascorbic acid on free radical tissue damage and inflammatory cell influx in the cornea after excimer laser corneal surgery. Archives of Ophthalmology, 117(5): 649-652.
Lalonde, R., Barraud, H., Ravey, J., Gueant, J.L., Bronowicki, J.P. and Strazielle, C. (2008). Effects of a B-vitamin-deficient diet on exploratory activity, motor coordination and spatial learning in young adult Balb/c mice. Annals of the New York Academy of Sciences, 118(8): 122-131.
Lalonde, R., Joyal, C.C. and Botez, M.I. (1993). Effects of folic acid and folinic acid on cognitive and motor behaviors in 20-month-old rats. Journal of Pharmacology, Biochemistry, and Behavior, 44(3): 703-707.
Lewis, C.A., Pancharuniti, N. and Sauberlich, H.E. (1992). Plasma folate adequacy as determined by homocysteine level. Annals of the New York Academy of Sciences, 66(9): 360-368.
Li Y., Feng, G., Yi Y. and Lin J. (1999). The experimental investigation of epithelial healing in rabbit central corneal alkali wounds. Annals of Eye Science, 15(2): 74-77.
Liu, R. and Pravia, K. (2010). Oxidative stress and glutathione in TGF-β-mediated fibrogenesis. Radical Biology and Medicine, 48(1): 1-15.
Maher, J.J. (2001). Interactions between hepatic stellate cells and the immune system. Seminars in Liver Disease, 21(3): 417-426.
Mannino, D.M., Mulinare, J., Ford, E.S. and Schwartz, J. (2003). Tobacco smoke exposure and decreased serum and red blood cell folate levels: data from the third national health and nutrition examination Survey. Nicotine and Tobacco Research, 5(3): 357-362.
Marsillach, J., Ferre, N., Camps, J., Riu, F., Rull, A. and Joven, J. (2008). Moderately high folic acid supplementation exacerbates experimentally induced liver fibrosis in rats. Radical Biology and Medicine, 233(1): 38-47.
Meller, D., Pires, R.T., Mack, R.G., Figueiredo, F., Heiligenhaus, A., Park, W.C., et al. (2000). Amniotic membrane transplantation for acute chemical or thermal burns. Ophthalmology, 107(5): 980-989.
Miller, A.L. (2003). The methionin-homocystein cycle and its effects on cognitive diseases. Scientific Review of Alternative Medicine, 63(8): 7-19.
Mills, J., Rhoads, G. and Simpson, J. (1989). The absence of a relationbetween the periconceptional use of vitamins and neural tube defects. The New England Journal of Medicine, 32(1): 430-435.
Nicholls, P. (1975). Formate as an inhibitor of cytochrome-C oxidase. Biochemical and Biophysical Research Communications, 67(2): 610-616.
Pietrzik, K., Bailey, L. and Shane, B. (2010). Folic acid and L-5-methyltetrahydrofolate. Clinical Pharmacokinetics, 49(8): 535-548.
Ponziani, F.R., Cazzato, I., Danese, S., Fagiuoli, S., Gionchetti, P., Annicchiarico, B., et al. (2012). Folate in gastrointestinal health and disease. European Review for Medical and Pharmacological Sciences, 16(3): 376-385.
Rinastiti, M., Harijadi, A., Santoso, A.L. and Sosroseno, W. (2006). Histological evaluation of rabbit gingival wound healing transplanted with human amniotic membrane. International Journal of Oral and Maxillofacial Surgery, 35(3): 247-51.
Selhub, J., Jacques, P.F., Wilson, P.W., Rush, D. and Rosenberg, I.H. (1993). Vitamin status and intake as primary determinants of homocysteinemia in an elderly population. Journal of the American Medical, 230(70): 269-438.
Seth, P., Kumari, R., Madhavan, S., Singh, A.K., Mani, H., Banaudha, K., et al. (2000). Prevention of renal ischemia reperfusion-induced injury in rats by picroliv. Biochemical Pharmacology, 59(10): 1315-1322.
Shimazaki, J., Yang, H.Y. and Tsubota, K. (1997). Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burns. Ophthalmology, 104(12): 2068-2076.
Shoham, A., Hadziahmetovic, M., Dunaief, J.L., Mydlarski, M.B. and Schipper, H.M. (2008). Oxidative stress in diseases of the human cornea. Radical Biology and Medicine, 45(8): 1047-1055.
Slatter, D. (2003). Eye and adnexa. In: Text book of Small Animal Surgery. Basher, T. editor. 2rd ed., Saunders, pp: 1354-1486.
Strubbe, D.T., Brooks, D.E., Schultz, G.S., Willis‐Goulet, H., Gelatt, K.N., Andrew, S.E., et al. (2000). Evaluation of tear film proteinases in horses with ulcerative keratitis. Veterinary Ophthalmology, 3(2-3): 111-119.
Unal, D., Yeni, E., Erel, O., Bitiren, M. and Vural, H. (2002). Antioxidative effects of exogenous nitric oxide versus antioxidant vitamins on renal ischemia reperfusion injury. Undergraduate Research Opportunities, 30(3): 190-194.
Walter, M.M., Shapior, S. and Mitchell, A.A. (1993). Periconceptional folic acidexposure and risk of oceurent tube defects. Journal of the American Medical, 269(10): 1257-1261.
Xiao-Wei, G., Xu-Dong, Z., Wen-Jing, L., Xin, Z. and Ying, L. (2012). Experimental study on the treatment of rabbit corneal melting after alkali burn with Collagen cross-linking. International Journal of Ophthalmology, 5(2): 147-150.
