نقش کاربرد براسینواسترویید بر تحمل به تنش کم آبی سرخارگل (.Echinacea purpurea L)

الموضوعات : اکوفیزیولوژی گیاهان زراعی

مطلب حسین پور ¹ , علی عبادی ² , اسماعیل نبی زاده ³ , حسن حبیبی ⁴ , سدابه جهانبخش گده کهریز ⁵

1 - دانشگاه محقق اردبیلی
2 - استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران
3 - استادیار گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران
4 - استادیار گروه زراعت و اصلاح نباتات، دانشکده علوم کشاورزی، دانشگاه شاهد، تهران، ایران
5 - دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

تاريخ الإرسال : 04 السبت , ربيع الأول, 1438 تاريخ التأكيد : 21 الأحد , ذو القعدة, 1438 تاريخ الإصدار : 15 السبت , ربيع الثاني, 1440

الکلمات المفتاحية: رنگدانه‌های فتوسنتزی, عملکرد اسانس, عملکرد اقتصادی, کارتنوئیدها,

ملخص المقالة :

خشکی می تواند اثر شدیدی بر رشد گیاهان داشته باشد. به منظور بررسی اثر کاربرد براسینواسترویید در تحمل به تنش کم آبی سرخارگل آزمایشی به‌صورت کرت های دو بار خرد شده بر پایه طرح بلوک های کامل تصادفی در سه تکرار و در شهرستان مهاباد اجرا شد. فاکتورهای مورد بررسی شامل رژیم آبیاری به عنوان فاکتور اصلی در سه سطح (آبیاری پس از 70، 120 و 170 میلی متر تبخیر از تشتک کلاس A)، کاربرد سورفکتانت (در دو سطح بدون و با غلظت نیم لیتر در هکتار) به عنوان فاکتور فرعی و محلول پاشی 24-اپی براسینواسترویید (در سه سطح شاهد (صفر)، 8-10 و 7-10 مولار) به عنوان فاکتور فرعی فرعی بودند. در این پژوهش صفات رنگدانه های فتوسنتزی (کلروفیل a، کلروفیل b، کلروفیل کل)، رنگدانه های کمکی (کارتنوئیدها، آنتوسیانین ها، فلانوئیدها و ترکیبات فنلی)، شاخص سبزینگی، هدایت روزنه ای، میزان مالون دی آلدهید، عملکرد اقتصادی (عملکرد ساقه، برگ و گل)، درصد اسانس و عملکرد اسانس اندازه گیری شدند. براسینواسترویید موجب کاهش اثرات مخرب تنش بر میزان کلروفیل a شد. تنش آبی میزان کلروفیل a را 28 درصد کاهش داد. با کاربرد براسینواسترویید (7-10 مولار) میزان تغییرات کلروفیل 35 درصد افزایش داشت. تنش آبی شاخص سبزینگی را 25 درصد کاهش داد. با کاربرد براسینواسترویید (7-10 مولار) شاخص سبزینگی 8 درصد افزایش یافت. کاربرد براسینواسترویید (7-10 مولار) موجب افزایش میزان کارتنوئیدها، آنتوسیانین ها و فلانوئیدها به ترتیب 17، 59 و 50 درصد شد. استفاده از غلظت 7-10 مولار براسینواسترویید در شرایط تنش باعث افزایش 13 درصدی در هدایت روزنه‌ای در مقایسه با عدم استفاده از آن گردید. نتایج رگرسیونی پیش بینی عملکرد نشان داد، هدایت روزنه ای، میزان مالون دی آلدهید و میزان کلروفیل a نقش مهمی در پیش بینی عملکرد دارند. نتایج این پژوهش نشان داد که تنش کم‌آبی سهم تعیین‌کننده‌ای بر کاهش فاکتورهای اساسی در رشد سرخارگل دارد؛ اما کاربرد براسینواسترویید و محلول سورفکتانت تحمل به تنش کم‌آبی را در این گیاه دارویی افزایش داد.

المصادر:

· Ahmadi Mosavi, E., KH. Kalantari, and M. Torkzade. 2005. Effects of 24-epibrassinolide on lipid peroxidation, prolin, sugar and photosynthesis pigments content of canola (Brassica napus L.) under water stress. Physiology. 18: 295-306. (In Persian).
· Ahmadian, A., A. Ghanbari, and M. Golvi. 2009. The Interaction effect of water Stress and animal manure on yield components, essential oil and chemical composition of Cuminum cyminum. Iranian Journal of Field Crop Science. 40(1): 173-180. (In Persian).
· Andalibi, B. 2009. The changes to the amount and composition of essential oil of Dill (Anethum graveolens) under limited irrigation conditions during growth and development. Ph.D. dissertation Agronomy, Faculty of Agriculture, University of Tabriz. (In Persian).
· Arnon, A.N. 1967. Method of extraction of chloroophll in the plants. Agronomy Journal. 23: 112-121.
· Asadisnam, S., M. Zavareh, H. Peardshti, F. Sefidkon, G.A. Nihmatzadeh, and A. Hashempoor. 2014. The effect of planting date and duration of waterlogging on some biochemical properties of leaves of purple coneflower (Echinacea purpurea (L.) Moench). National Conference on Medicinal Plants Educators and Technologists. Twenty-ninth and thirtieth November Islamic Azad University, Science and Research Ayatollah Amoli. Amol. Iran. (In Persian).
· Azizi, M., F. Rezwanee, M. Hassanzadeh Khayat, A. Lackzian, and H. Neamati. 2009. The effect of different levels of vermicompost and irrigation on morphological properties and essential oil content of german chamomile (Matricaria recutita) C.V. Goral. Iranian Jouranal of Meadicial and Aromatic Plant. 24(1): 82-93. (In Persian).
· Bajgaz, A. 2000. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of chorella vulgaris. Plant Physiology and Biochemistery. 38: 209-215.
· Balouchi, H.R., S.A. Modarres Sanavy, Y. Emam, and M. Barzegar. 2008. Effect of water deficit, ultraviolet radiation and carbon dioxide enrichment on flag leaf qualitative characters of durum wheat (Triticum turgidum L.). Journal of Agricultural Science and Technology and Natural Resources. 12 (45): 167-181. (In Persain).
· Bazzazi, N., M. Khodambashi, and SH. Mohammadi.2013. The Effect of drought stress on morphological characteristics and yield components of medicinal plant fenugreek. Journal of Crop Production and Processing. 8(3): 11-23. (In Persian).
· Behnamnia, M., Kh.M. Kalantari,and F. Rezanejad. 2009. Exogenous application of brassinosteroid alleviates drought-induced oxidativestress in Lycopersicon esculentum L. General and Applied Plant Physiology. 35 (1-2): 22-34.
· Bettaieb, I., N. Zakhama, N. Aidi-Wannes, M.E. Kchouk, and B. Marzouk. 2009. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Scientia Horticulturae. 120: 271-275.
· Chaichi, M.R., P. Nurre, J. Slaven, and M. Rostamza. 2015. Surfactant application on yield and irrigation water use efficiency in corn under limited irrigation. Crop Science. 55: 386-393.
· Daneshnia, F., and M.R. Chaichi. 2014. The effect of surfactant application and limited irrigation systems on qualitative traits of medicinal forage (Case Study: berseem clover and basil intercropping). 1^stInternational and 13^thIranian Crop Science Congress 3 ^rd Iranian Seed Science and Technology Conference. Karaj, Iran. (In Persian).
· De Vos, C., H. Schat, M. De Waal, R. Vooijs, and W. Ernst. 1991. Increased to copperinduced damage of the root plasma membrane in copper tolerant silene cucubalus. PlantPhysiology. 82: 523-528.
· Eiasu, B.K., J.M. Steyn, and P. Soundy. 2012. Physiomorphological response of rose-scented geranium (Pelargonium spp.) to irrigation frequency. South African Journal of Botany. 78: 96-103.
· Ekrena, S., C. Sonmez, E. Ozcakal, Y.S.K. Kurttas, E. Bayram, and H. Gurgulu. 2012. The effect of different irrigation water levels on yield and quality characteristics of purple basil (Ocimum basilicum L.). Agricultural Water Management. 109: 155-161.
· Eskandari, M. 2011. Effect of 28-hemobrassinolid to reducing the effects of drought in savory herbs. Plant Physiology and Biochemistry. 3(11): 183-187.
· FathiAmirkhiz, K., M. Amini Dehaghi, and S.Heshmati. 2015. Study the effect of iron chelate on chlorophyll content, photochemical efficiency and some biochemical traits in safflower under deficit irrigation condition. Iranian Journal of Field Crop Science. 46(1): 137-145. (In Persian).
· Figueiredo, A.C., J.G. Barroso, L.G. Pedro, and J.J. Scheffer. 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal. 23: 213-226.
· Fu, J., and B. Huang. 2001. Involvement of antioxidant and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany. 45: 105-114.
· Gautam, P.P., A.K. Fritz, M.B.K. Kirkham, and B. Gill. 2011. Response of Aegilops species to drought stress during reproductive stages of development. Fundamental for Life. Soil, Crop & Environmental Sciences. Internatinal Annual Meetings. 16-19.
· Ghorbanali, M., and M. Niakan. 2005. Evaluation of drought stress effect on soluble sugars content, protein, prolin and phenolic compounds and nitrate reeducates activity in soybean cultivar Gorgan 3. Iranian Journal of Science Education. 1(2): 537-550. (In Persian).
· Grace, S.C., and B.A. Logan. 2000. Energy dissipation and radical scavenging by the plant phenyl propanoid pathway. Philosophical Transactions of the Royal Society B. 355: 1499-1510.· Habibi, H., D. Mazaheri, N. Majnoon Hosseini, M.R. Chaichi, M. Tabatabai, and M. Bigdeli, 2011. Evaluation of the impact of organic sources (biological) and inorganic nitrogen (urea) on performance and secondary metabolites both wild and cultivated plant species of thyme (Thymus spp.). Ph.D. Thesis. Department of Crop and Animal Sciences, College of Agriculture and Natural Resources, Tehran University.129 pp (In Persian).
· Hanstein, S.M., and H.H. Felle. 2002. CO₂-triggered chloride release from guard cells in intact faba bean leaves. Kinetics of the onset of stomatal closure. Plant Physiology. 130: 940-950.
· Hataei, Sh. 2004. The effect of drought on physiological characteristics and activity of antioxidant enzymes in different cultivars of peas. M.S. dissertation. Agricultural faculty in Karaj, Islamic Azad University of Karaj. 150 pp. (In Persian).
· Heidari Sharif-Abad, H. 2000. Plants, aridity, and drought. Research Institute of Forests and Rangelans, Tehran, Iran. 200pp. (In Persian).
· Inze, D., and M.V. Montagu. 2000 Oxidative stress in plants. T.J. International Ltd, Padstow, Carnawell. Great Britain. 321pp
· Jaleel, C.A., R. Gopi, B. Sankar, M. Gomathinayagam, and R. Panneerselvam. 2008. Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress. Comptes Rendus Biologies. 33: 42-47.
· Jin, J., S. Ningwei, B. Jinhe, and G. Junping. 2006. Regulation of ascorbate peroxidase the transcript level is involved in tolerance to postharvest water deficit stress in the cut Rose (Rose Hybrida L.) cv. Samantha. Postharvest Biology and Technology. 40: 236-243.
· Khazaie, H.R., F. Nadjafib, and M. Bannayan. 2008. Effect of irrigation frequency and planting density on herbage biomass and oil production of thyme (Thymus vulgaris) and hyssop (Hyssopus officinalis). Industrial Crops and Products. 27: 315–321.
· Krizek, D.T., S.J. Britz, and R.M. Mirecki. 1998. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of, New Red Fire Lettuce. Physiology Plant. 103: 1-7.
· Lebaschy, M.H., and E. Sharifi Ashoorabadi. 2004. Growth indices of some medicinal plants under different water stresses. Iranian Journal of Medicinal and Aromatic Plants Research. 20(3): 249-261. (In Persian).
· Leng, P., H. Itamura, H. Yamamura, and M.X. Deng, 2000. Anthocyanin accumulation in apple and peach shoots during cold acclimation. Scientia Horticulturae. 83: 43-50.
· Letchamo, W., L.V. Polydeonny, N.O. Gladisheva, and T.J. Arnason. 2002 Factors affecting Echinacea quality. In: Trends in new crops and new uses (eds. Janick J, Whipkey A.). 514 pp. ASHS Press: Alexandria VA.
· Li, K.R., H.H. Wang, G. Han, Q.J. Wang, and J. Fan. 2008. Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. New Forests. 35: 255-266.
· Meri, H.R. 2004. Physiology and yield of crops plants under drought stress. Press the Navid of Shiraz. (In Persian).
· Motsa, N.M., P. Soundy, J.M. Steyn, R.A. Learmonth, N.Mojela, and C. Teubes. 2006. Plant shoot age and temperature effects on essential oil yield and oil composition of rose-scented geranium (Pelargonium sp.) grown in South Africa. Journal of Essential Oil Research. 18: 106-110.
· Mrozikiewicz, P.M., A. Bogacz, M. Karasiewicz, P.L. Mikolajczak, M. Ozarowski, A. Seremak-Mrozikiewicz, B. Czerny, T. Bobkiewicz-Kozlowska, and E. Grzeskowiak. 2010. The effect of standardized Echinacea purpurea extracts on rat cytochrome P450 expression level. Phytomedicine. 17: 830-833.
· Navrozpoor, Gh., and P. Rezvanimqadam. 2006. The effect of irrigation intervals and plant density on yield and essential oil of black seed oil (Nigella sativa L.). Research and Development in Agriculture and Horticulture. 73:133-138. (In Persian).
· Norzad, S., A. Ahmadian, M. Moghaddam, and E. Daneshfar. 2014. Effect of drought stress on yield, yield components and essential oil in coriander treated with organic and inorganic fertilizers. Agricultural Crop Management. 16(2): 289-302. (In Persian).
· Omidbaigi, R. 2005. Production and processing of medicinal plants. Tehran University. 283 pp. (In Persian).
· Ouerghi, Z., G. Cornie, M. Roudani, A. Ayadi, and J. Bruifert. 2000. Effect of NaCl on the photosynthesis of two wheat species (Triticum durum and T. aestivum) differing in their sensitivity to salt stress. Plant Physiology. 156: 335-340.
· Ozdamir, F., M. Bor, T. Demiral, and I. Turkan. 2012. Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and anti oxidative system of rice (Oriza sativa L.) under salinity stress. Plant Growth Regulation. 42: 203-211.
· Raziqiedk, W. 2006. Effect of salinity and drought stresses on phosphatase enzyme activity in bread wheat cultivars in the early stages of seed germination. M.S. Dissertation. Tehran University. 150 pp. (In Persian).
· Rezaei Chaine, A. 2012. The effect of irrigation on the accumulation of essential oil, its composition and some physiological traits in fennel. Ph.D. Thesis Agronomy, Faculty of Agriculture, University of Tabriz. 93pp. (In Persian).
· Rezaei Chaine, A., S. Zehtab Salmasi, K. Ghasemi Golezani, and H. Dlazr. .2012. The effects on yield and yield components of fennel landraces. Journal of Sustainable Agricultural Knowledge. 22(4): 55-70. (In Persian).
· Rezapour, H., M. Haider, M. Glovi, and M. Ramroudi. 2011. Effect of drought stress and different amounts of sulfur fertilizer on yield and yield components and osmotic adjustment in medicinal plant buckwheat. Quarterly Journal of Medicinal and Aromatic Plants of Iran Research. 27(3): 396-384. (In Persian).
· Ronald, S.F., and S.K. Laima. 1999. Phenolics and cold tolerance of Brassica napus. Plant Agriculture. 1: 1-5.
· Sabra, A., F. Daayf, and S. Renault. 2012. Differential physiological and biochemical responses of three Echinacea species to salinity stress. Scientia Horticulturae. 135: 23-31.
· Shahmoradi, Sh. 2003. Effects of drought stress on qualitative and quantitative traits of soybean cultivars and advanced Layn‌Hay. Tehran University, Tehran. M.Sc. Thesis. 104pp. (In Persian).
· Siosemardeh, A., A. Ahmadi, K. Poustini, and H. Ebrahimzadeh, 2003. Stomatal and nonstomatal limitations to photosynthesis and their relationship with drought resistance in wheat cultivars. Iranian Journal of Agricultural Science. 35(1): 93-106. (In Persian).
· Sofo, A., A.C. Tuzio, B. Dichio, and C. Xiloyannis. 2005. Influence of water deficit and rewatering on the components of the ascorbate-gluta-thione cycle in four interspecific Prunus hybrids. Plant Science. 69: 403-412.
· Swamy, K.N., and S.S.R. Rao. 2009. Effect of 24-Epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit. Russian Journal of Plant Physiology. 56(5): 616-620.
· Tsai, Y.L., S.Y. Chiou, K.C. Chan, and J.M. Sung. 2012. Caffeic acid derivatives, total phenols, antioxidant and antimutagenic activities of Echinacea purpurea flower extracts. LWT-Food Science and Technology. 46: 169-176.
· Wagner, G. J. 1979. Content and vacuole /extera vacuole distribution of neutral sugars, free amino acids and anthocyanin in protoplast. Plant Physiology. 68: 88-93.
· Wang, Z., and B. Huang. 2004. Physiological recovery of Kentucky bluegrass from simultaneous drought and heat stress. Crop Science. 44: 1729-1736.
· Xiao X., X. Xu, and F. Yang. 2008. Adaptive responses to progressive drought stress in two Populus cothayana populations. Silva Fennica. 42: 705-719.
· Yang, J., J. Zhang, Z. Wang, Q. Zhu, and L. Liu. 2001. Water deficit induced senescence and its relationship to the remobilization of pre-stored carbon in wheat during grain filling. Agronomy Journal. 93: 196-206.
· Yang, X. 2008. Effects of nonionic surfactant on plant growth and physiology. Ph.D. Thesis. Faculty of University Auburn of Alabama. 141pp.
· Zhang, M., Z. Zhai, X. Tian, L. Duan, and Z. Li. 2008. Brassinolide alleviated the adverse effect of water deficits on photosynthesis and the antioxidant of soybean (Glycine max L.). Plant Growth Regulation. 56: 257-264.
Zhuoyun, W., and L. Jia. 2016. Brassinosteroids regulate root growth, development, and symbiosis. Molecular Plant. 9(1): 86-100.

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· Ahmadi Mosavi, E., KH. Kalantari, and M. Torkzade. 2005. Effects of 24-epibrassinolide on lipid peroxidation, prolin, sugar and photosynthesis pigments content of canola (Brassica napus L.) under water stress. Physiology. 18: 295-306. (In Persian).
· Ahmadian, A., A. Ghanbari, and M. Golvi. 2009. The Interaction effect of water Stress and animal manure on yield components, essential oil and chemical composition of Cuminum cyminum. Iranian Journal of Field Crop Science. 40(1): 173-180. (In Persian).
· Andalibi, B. 2009. The changes to the amount and composition of essential oil of Dill (Anethum graveolens) under limited irrigation conditions during growth and development. Ph.D. dissertation Agronomy, Faculty of Agriculture, University of Tabriz. (In Persian).
· Arnon, A.N. 1967. Method of extraction of chloroophll in the plants. Agronomy Journal. 23: 112-121.
· Asadisnam, S., M. Zavareh, H. Peardshti, F. Sefidkon, G.A. Nihmatzadeh, and A. Hashempoor. 2014. The effect of planting date and duration of waterlogging on some biochemical properties of leaves of purple coneflower (Echinacea purpurea (L.) Moench). National Conference on Medicinal Plants Educators and Technologists. Twenty-ninth and thirtieth November Islamic Azad University, Science and Research Ayatollah Amoli. Amol. Iran. (In Persian).
· Azizi, M., F. Rezwanee, M. Hassanzadeh Khayat, A. Lackzian, and H. Neamati. 2009. The effect of different levels of vermicompost and irrigation on morphological properties and essential oil content of german chamomile (Matricaria recutita) C.V. Goral. Iranian Jouranal of Meadicial and Aromatic Plant. 24(1): 82-93. (In Persian).
· Bajgaz, A. 2000. Effect of brassinosteroids on nucleic acids and protein content in cultured cells of chorella vulgaris. Plant Physiology and Biochemistery. 38: 209-215.
· Balouchi, H.R., S.A. Modarres Sanavy, Y. Emam, and M. Barzegar. 2008. Effect of water deficit, ultraviolet radiation and carbon dioxide enrichment on flag leaf qualitative characters of durum wheat (Triticum turgidum L.). Journal of Agricultural Science and Technology and Natural Resources. 12 (45): 167-181. (In Persain).
· Bazzazi, N., M. Khodambashi, and SH. Mohammadi.2013. The Effect of drought stress on morphological characteristics and yield components of medicinal plant fenugreek. Journal of Crop Production and Processing. 8(3): 11-23. (In Persian).
· Behnamnia, M., Kh.M. Kalantari,and F. Rezanejad. 2009. Exogenous application of brassinosteroid alleviates drought-induced oxidativestress in Lycopersicon esculentum L. General and Applied Plant Physiology. 35 (1-2): 22-34.
· Bettaieb, I., N. Zakhama, N. Aidi-Wannes, M.E. Kchouk, and B. Marzouk. 2009. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Scientia Horticulturae. 120: 271-275.
· Chaichi, M.R., P. Nurre, J. Slaven, and M. Rostamza. 2015. Surfactant application on yield and irrigation water use efficiency in corn under limited irrigation. Crop Science. 55: 386-393.
· Daneshnia, F., and M.R. Chaichi. 2014. The effect of surfactant application and limited irrigation systems on qualitative traits of medicinal forage (Case Study: berseem clover and basil intercropping). 1^stInternational and 13^thIranian Crop Science Congress 3 ^rd Iranian Seed Science and Technology Conference. Karaj, Iran. (In Persian).
· De Vos, C., H. Schat, M. De Waal, R. Vooijs, and W. Ernst. 1991. Increased to copperinduced damage of the root plasma membrane in copper tolerant silene cucubalus. PlantPhysiology. 82: 523-528.
· Eiasu, B.K., J.M. Steyn, and P. Soundy. 2012. Physiomorphological response of rose-scented geranium (Pelargonium spp.) to irrigation frequency. South African Journal of Botany. 78: 96-103.
· Ekrena, S., C. Sonmez, E. Ozcakal, Y.S.K. Kurttas, E. Bayram, and H. Gurgulu. 2012. The effect of different irrigation water levels on yield and quality characteristics of purple basil (Ocimum basilicum L.). Agricultural Water Management. 109: 155-161.
· Eskandari, M. 2011. Effect of 28-hemobrassinolid to reducing the effects of drought in savory herbs. Plant Physiology and Biochemistry. 3(11): 183-187.
· FathiAmirkhiz, K., M. Amini Dehaghi, and S.Heshmati. 2015. Study the effect of iron chelate on chlorophyll content, photochemical efficiency and some biochemical traits in safflower under deficit irrigation condition. Iranian Journal of Field Crop Science. 46(1): 137-145. (In Persian).
· Figueiredo, A.C., J.G. Barroso, L.G. Pedro, and J.J. Scheffer. 2008. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour and Fragrance Journal. 23: 213-226.
· Fu, J., and B. Huang. 2001. Involvement of antioxidant and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany. 45: 105-114.
· Gautam, P.P., A.K. Fritz, M.B.K. Kirkham, and B. Gill. 2011. Response of Aegilops species to drought stress during reproductive stages of development. Fundamental for Life. Soil, Crop & Environmental Sciences. Internatinal Annual Meetings. 16-19.
· Ghorbanali, M., and M. Niakan. 2005. Evaluation of drought stress effect on soluble sugars content, protein, prolin and phenolic compounds and nitrate reeducates activity in soybean cultivar Gorgan 3. Iranian Journal of Science Education. 1(2): 537-550. (In Persian).
· Grace, S.C., and B.A. Logan. 2000. Energy dissipation and radical scavenging by the plant phenyl propanoid pathway. Philosophical Transactions of the Royal Society B. 355: 1499-1510.· Habibi, H., D. Mazaheri, N. Majnoon Hosseini, M.R. Chaichi, M. Tabatabai, and M. Bigdeli, 2011. Evaluation of the impact of organic sources (biological) and inorganic nitrogen (urea) on performance and secondary metabolites both wild and cultivated plant species of thyme (Thymus spp.). Ph.D. Thesis. Department of Crop and Animal Sciences, College of Agriculture and Natural Resources, Tehran University.129 pp (In Persian).
· Hanstein, S.M., and H.H. Felle. 2002. CO₂-triggered chloride release from guard cells in intact faba bean leaves. Kinetics of the onset of stomatal closure. Plant Physiology. 130: 940-950.
· Hataei, Sh. 2004. The effect of drought on physiological characteristics and activity of antioxidant enzymes in different cultivars of peas. M.S. dissertation. Agricultural faculty in Karaj, Islamic Azad University of Karaj. 150 pp. (In Persian).
· Heidari Sharif-Abad, H. 2000. Plants, aridity, and drought. Research Institute of Forests and Rangelans, Tehran, Iran. 200pp. (In Persian).
· Inze, D., and M.V. Montagu. 2000 Oxidative stress in plants. T.J. International Ltd, Padstow, Carnawell. Great Britain. 321pp
· Jaleel, C.A., R. Gopi, B. Sankar, M. Gomathinayagam, and R. Panneerselvam. 2008. Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress. Comptes Rendus Biologies. 33: 42-47.
· Jin, J., S. Ningwei, B. Jinhe, and G. Junping. 2006. Regulation of ascorbate peroxidase the transcript level is involved in tolerance to postharvest water deficit stress in the cut Rose (Rose Hybrida L.) cv. Samantha. Postharvest Biology and Technology. 40: 236-243.
· Khazaie, H.R., F. Nadjafib, and M. Bannayan. 2008. Effect of irrigation frequency and planting density on herbage biomass and oil production of thyme (Thymus vulgaris) and hyssop (Hyssopus officinalis). Industrial Crops and Products. 27: 315–321.
· Krizek, D.T., S.J. Britz, and R.M. Mirecki. 1998. Inhibitory effects of ambient levels of solar UV-A and UV-B radiation on growth of, New Red Fire Lettuce. Physiology Plant. 103: 1-7.
· Lebaschy, M.H., and E. Sharifi Ashoorabadi. 2004. Growth indices of some medicinal plants under different water stresses. Iranian Journal of Medicinal and Aromatic Plants Research. 20(3): 249-261. (In Persian).
· Leng, P., H. Itamura, H. Yamamura, and M.X. Deng, 2000. Anthocyanin accumulation in apple and peach shoots during cold acclimation. Scientia Horticulturae. 83: 43-50.
· Letchamo, W., L.V. Polydeonny, N.O. Gladisheva, and T.J. Arnason. 2002 Factors affecting Echinacea quality. In: Trends in new crops and new uses (eds. Janick J, Whipkey A.). 514 pp. ASHS Press: Alexandria VA.
· Li, K.R., H.H. Wang, G. Han, Q.J. Wang, and J. Fan. 2008. Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. New Forests. 35: 255-266.
· Meri, H.R. 2004. Physiology and yield of crops plants under drought stress. Press the Navid of Shiraz. (In Persian).
· Motsa, N.M., P. Soundy, J.M. Steyn, R.A. Learmonth, N.Mojela, and C. Teubes. 2006. Plant shoot age and temperature effects on essential oil yield and oil composition of rose-scented geranium (Pelargonium sp.) grown in South Africa. Journal of Essential Oil Research. 18: 106-110.
· Mrozikiewicz, P.M., A. Bogacz, M. Karasiewicz, P.L. Mikolajczak, M. Ozarowski, A. Seremak-Mrozikiewicz, B. Czerny, T. Bobkiewicz-Kozlowska, and E. Grzeskowiak. 2010. The effect of standardized Echinacea purpurea extracts on rat cytochrome P450 expression level. Phytomedicine. 17: 830-833.
· Navrozpoor, Gh., and P. Rezvanimqadam. 2006. The effect of irrigation intervals and plant density on yield and essential oil of black seed oil (Nigella sativa L.). Research and Development in Agriculture and Horticulture. 73:133-138. (In Persian).
· Norzad, S., A. Ahmadian, M. Moghaddam, and E. Daneshfar. 2014. Effect of drought stress on yield, yield components and essential oil in coriander treated with organic and inorganic fertilizers. Agricultural Crop Management. 16(2): 289-302. (In Persian).
· Omidbaigi, R. 2005. Production and processing of medicinal plants. Tehran University. 283 pp. (In Persian).
· Ouerghi, Z., G. Cornie, M. Roudani, A. Ayadi, and J. Bruifert. 2000. Effect of NaCl on the photosynthesis of two wheat species (Triticum durum and T. aestivum) differing in their sensitivity to salt stress. Plant Physiology. 156: 335-340.
· Ozdamir, F., M. Bor, T. Demiral, and I. Turkan. 2012. Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and anti oxidative system of rice (Oriza sativa L.) under salinity stress. Plant Growth Regulation. 42: 203-211.
· Raziqiedk, W. 2006. Effect of salinity and drought stresses on phosphatase enzyme activity in bread wheat cultivars in the early stages of seed germination. M.S. Dissertation. Tehran University. 150 pp. (In Persian).
· Rezaei Chaine, A. 2012. The effect of irrigation on the accumulation of essential oil, its composition and some physiological traits in fennel. Ph.D. Thesis Agronomy, Faculty of Agriculture, University of Tabriz. 93pp. (In Persian).
· Rezaei Chaine, A., S. Zehtab Salmasi, K. Ghasemi Golezani, and H. Dlazr. .2012. The effects on yield and yield components of fennel landraces. Journal of Sustainable Agricultural Knowledge. 22(4): 55-70. (In Persian).
· Rezapour, H., M. Haider, M. Glovi, and M. Ramroudi. 2011. Effect of drought stress and different amounts of sulfur fertilizer on yield and yield components and osmotic adjustment in medicinal plant buckwheat. Quarterly Journal of Medicinal and Aromatic Plants of Iran Research. 27(3): 396-384. (In Persian).
· Ronald, S.F., and S.K. Laima. 1999. Phenolics and cold tolerance of Brassica napus. Plant Agriculture. 1: 1-5.
· Sabra, A., F. Daayf, and S. Renault. 2012. Differential physiological and biochemical responses of three Echinacea species to salinity stress. Scientia Horticulturae. 135: 23-31.
· Shahmoradi, Sh. 2003. Effects of drought stress on qualitative and quantitative traits of soybean cultivars and advanced Layn‌Hay. Tehran University, Tehran. M.Sc. Thesis. 104pp. (In Persian).
· Siosemardeh, A., A. Ahmadi, K. Poustini, and H. Ebrahimzadeh, 2003. Stomatal and nonstomatal limitations to photosynthesis and their relationship with drought resistance in wheat cultivars. Iranian Journal of Agricultural Science. 35(1): 93-106. (In Persian).
· Sofo, A., A.C. Tuzio, B. Dichio, and C. Xiloyannis. 2005. Influence of water deficit and rewatering on the components of the ascorbate-gluta-thione cycle in four interspecific Prunus hybrids. Plant Science. 69: 403-412.
· Swamy, K.N., and S.S.R. Rao. 2009. Effect of 24-Epibrassinolide on growth, photosynthesis, and essential oil content of Pelargonium graveolens (L.) Herit. Russian Journal of Plant Physiology. 56(5): 616-620.
· Tsai, Y.L., S.Y. Chiou, K.C. Chan, and J.M. Sung. 2012. Caffeic acid derivatives, total phenols, antioxidant and antimutagenic activities of Echinacea purpurea flower extracts. LWT-Food Science and Technology. 46: 169-176.
· Wagner, G. J. 1979. Content and vacuole /extera vacuole distribution of neutral sugars, free amino acids and anthocyanin in protoplast. Plant Physiology. 68: 88-93.
· Wang, Z., and B. Huang. 2004. Physiological recovery of Kentucky bluegrass from simultaneous drought and heat stress. Crop Science. 44: 1729-1736.
· Xiao X., X. Xu, and F. Yang. 2008. Adaptive responses to progressive drought stress in two Populus cothayana populations. Silva Fennica. 42: 705-719.
· Yang, J., J. Zhang, Z. Wang, Q. Zhu, and L. Liu. 2001. Water deficit induced senescence and its relationship to the remobilization of pre-stored carbon in wheat during grain filling. Agronomy Journal. 93: 196-206.
· Yang, X. 2008. Effects of nonionic surfactant on plant growth and physiology. Ph.D. Thesis. Faculty of University Auburn of Alabama. 141pp.
· Zhang, M., Z. Zhai, X. Tian, L. Duan, and Z. Li. 2008. Brassinolide alleviated the adverse effect of water deficits on photosynthesis and the antioxidant of soybean (Glycine max L.). Plant Growth Regulation. 56: 257-264.
Zhuoyun, W., and L. Jia. 2016. Brassinosteroids regulate root growth, development, and symbiosis. Molecular Plant. 9(1): 86-100.