Molecular Identification of Gelatin Origin in Pastilles and Jelly Products Collected from Tehran Markets
الموضوعات :N. Kamandi 1 , M. Ghobadi Dana 2 , M. Ghavami 3
1 - MSc Graduated of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor of Microbiology Research Group, Faculty of Food Science and Agriculture, Standard Research Institute, Karaj, Iran.
3 - Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
الکلمات المفتاحية: Fraud detection, Gelatin, Halal Authentication, Jelly Product, Qualitative PCR, Species Identification,
ملخص المقالة :
Gelatin is a beneficial component in the structure of various foods such as desserts and pastilles. These products are most popular with children because of their visual appeal and bright colors. In this study, 30 samples of jelly products were collected from the Tehran markets and were tested for the molecular identification of the gelatin source. The gelatin used in the industry is mainly produced from bovine and porcine species. Identification and detection of the species used in the gelatin structure are required from the perspective of consumer confidence in food health safety as well as religious beliefs. A species-specific singleplex PCR reaction targeting 277 bp porcine D-loop regions was used to detect porcine species. It was found that none of the products contained porcine gelatin. Complementary experiments used with 130 bp bovine mitochondrial DNA (mtDNA) to detect bovine species showed that the collected samples, according to the label, contained bovine species DNA. Therefore, this qualitative PCR assay is a useful and effective method for monitoring gelatin species origin in food products and has the ability to identifying the DNA of the porcine in gelatin as a highly processed food product and Halal authentication.
Abbas, O., Zadravec, M., Baeten, V., Mikuš, T., Lešić, T., Vulić, A. & Pleadin, J. (2018). Analytical methods used for the authentication of food of animal origin. Food Chemistry, 246, 6-17.
Aida, A. A., Man, Y. C., Wong, C. M. V. L., Raha, A. R. & Son, R. )2005). Analysis of raw meats and fats of pigs using polymerase chain reaction for Halal authentication. Meat Science, 69(1), 47-52.
Ali, M. E., Al Amin, M., Razzak, M. A., Abd Hamid, S. B., Rahman, M. M., Abdul Rashid, N. & Asing. (2015). Short Amplicon-Length PCR Assay Targeting Mitochondrial Cytochrome b Gene for the Detection of Feline Meats in Burger Formulation. Food Analytical Methods, 9(3), 571-581.
Anon. (2011) US Pharmacopeial Convention. Rockville, MD, 4403-4404.
Anon. (2020). https://www.technavio.com/report/gelatin-market-industry-nalysis.
Anon. (2020). Tehran chamber of commerce, industries, mines and agriculture
http://www.tccim.ir/ImpExpStats_TarrifCustomCountry.aspx?slcImpExp=Import&slcCountry=&sYear=1397&mode=doit
Che Man, Y. B., Aida, A. A., Raha, A. R. & Son, R. (2007). Identification of pork
derivatives in food products by species-specific polymerase chain reaction )PCR) for halal verification. Food Control, 18(7), 885-889.
Che Man, Y. B., Mustafa, S., Khairil Mokhtar, N. F., Nordin, R. & Sazili, A. Q. (2012). Porcine-specific polymerase chain reaction assay based on mitochondrial D-loop gene for identification of pork in raw meat.
International Journal of Food Properties, 15(1), 134-144.
Dalmasso, A., Fontanella, E., Piatti, P., Civera, T., Rosati, S. & Bottero, M. T. (2004). A multiplex PCR assay for the identification of animal species in feed stuffs. Molecular and Cellular Probes, 18(2), 81-87.
Demirhan, Y., Ulca, P. & Senyuva, H. Z. (2012). Detection of porcine DNA in gelatine and gelatine-containing processed food products-Halal/Kosher authentication. Meat Science, 90(3), 686-689.
Doosti, A., Ghasemi Dehkordi, P. & Rahimi, E. (2014). Molecular assay to fraud identification of meat products. Journal of Food Science and Technology, 51(1), 148-52.
Fajardo, V., González, I., Rojas, M., García, T. & Martín, R. (2010). A review of
current PCR-based methodologies for the authentication of meats from game animal species. Trends in Food Science & Technology, 21(8), 408-421.
GIMA, G. G. (2012). Gelatin Manufacturers Institute of America. New York, NY, USA.
Girish, P., Anjaneyulu, A., Viswas, K., Anand, M., Rajkumar, N., Shivakumar, B. &
Bhaskar, S. (2004). Sequence analysis of mitochondrial 12S rRNA gene can identify meat species. Meat Science, 66(3), 551-556.
Ishaq, A., ur Rahman, U., Sahar, A., Perveen, R., Deering, A. J., Khalil, A. A. & Siddique, U. (2019). Potentiality of analytical approaches to determine gelatin authenticity in food systems: A review. LWT, 108968.
Kang, S. S. N., Lee, H. G. & Kim, H. (2018). Development and comparison of a porcine gelatin detection system targeting mitochondrial markers for Halal authentication. LWT, 97, 697-702.
Lee, J. H., Kim, M.-R., Jo, C. H., Jung, Y. K., Kwon, K. & Kang, T. S. (2016). Specific PCR assays to determine bovine, porcine, fish and plant origin of gelatin capsules of dietary supplements. Food Chemistry, 211, 253-259.
Mafra, I., Ferreira, I. M. & Oliveira, M. B. P. (2008). Food authentication by PCR-based methods. European Food Research and Technology, 227(3), 649–665
Mane, B. G., Mendiratta, S. K. & Tiwari, A. K. (2012). Beef specific polymerase chain reaction assay for authentication of meat and meat products. Food Control, 28(2), 246-249.
Nikzad, J., Shahhosseini, S., Tabarzad, M., Nafissi-Varcheh, N. & Torshabi, M. (2017). Simultaneous detection of bovine and porcine DNA in pharmaceutical gelatin capsules by duplex PCR assay for Halal authentication. DARU Journal of Pharmaceutical Sciences, 25(1), 3.
Rao, M. S., Chakraborty, G. & Murthy, K. S. (2019). Market Drivers and Discovering Technologies in Meat Species Identification. Food Analytical Methods, 12(11), 2416-2429.
Rodríguez, M. A., García, T., González, I., Asensio, L., Hernandez, P. E. & Martin, R. (2004). PCR identification of beef, sheep, goat, and pork in raw and heat-treated meat mixtures. Journal of Food Protection, 67(1), 172-177.
Rohman, A., Windarsih, A., Erwanto, Y. & Zakaria, Z. (2020). Review on analytical methods for analysis of porcine gelatine in food and pharmaceutical products for halal authentication. Trends in Food Science and Technology, 101, 122-132.
Sahilah, A. M., Norhayati, Y., Norrakiah, A. S., Aminah, A. & Wan Aida, W. M. (2011). Halal authentication of raw meats using PCR amplification of mitchondrial DNA. International Food Research Journal, 18(4), 1481-1491.
Sahilah, A. M., Fadly, M. L., Norrakiah, A. S., Aminah, A., Aida, W. W., Ma'aruf, A. G. & Khan, M. A. (2012). Halal market surveillance of soft and hard gel capsules in pharmaceutical products using PCR and southern-hybridization on the biochip analysis. International Food Research Journal, 19(1), 371-375.
Song, K. Y., Hwang, H. J. & Kim, J. H. (2017). Ultra-fast DNA-based multiplex convection PCR method for meat species identification with possible on-site applications. Food chemistry, 229, 341-346.
Sultana, S., Hossain, M. M., Naquiah, N. N. A. & Ali, M. E. (2018-a). Novel multiplex PCR-RFLP assay discriminates bovine, porcine and fish gelatin substitution in Asian pharmaceuticals capsule shells. Food Additives and Contaminants: Part A, 35(9), 1662-1673.
Sultana, S., Hossain, M. M., Zaidul, I. S. M. & Ali, M. E. (2018-b). Multiplex PCR to discriminate bovine, porcine, and fish DNA in gelatin and confectionery products. LWT, 92, 169-176.
Sultana, S., Hossain, M. M., Azlan, A., Johan, M. R., Chowdhury, Z. Z. & Ali, M. E. (2020). TaqMan probe based multiplex quantitative PCR assay for determination of bovine, porcine and fish DNA in gelatin admixture, food products and dietary supplements. Food Chemistry, 126756.
Tasara, T., Schumacher, S. & Stephan, R. (2005). Conventional and real-time PCR-based approaches for molecular detection and quantitation of bovine species material in edible gelatin. Journal of Food Protection, 68(11), 2420-2426.
Wang, Q., Cai, Y., He, Y., Yang, L. & Pan, L. (2018). Collaborative ring trial of two real-time PCR assays for the detection of porcine-and chicken-derived material in meat products. Plos One, 13(10), e0206609.
