Evaluation of Hydrolyzed Black Cumin Seed Protein as a Bioactive Peptide Source in Broiler Chicken Diets
الموضوعات :A. Dadashi Oranj 1 , B. Navidshad 2 , S. Karimzadeh 3 , A. Kalantari Hesari 4 , F. Mirzaei Aghjehgheshlagh 5
1 - Department of Animal Science, Faculty of Agricultural Science and Technology, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, Faculty of Agricultural Science and Technology, University of Mohaghegh Ardabili, Ardabil, Iran
3 - Department of Animal Science, Rodaki Higher Education Institute, Tonekabon, Mazandaran, Iran
4 - Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran
5 - Department of Animal Science, Faculty of Agricultural Science and Technology, University of Mohaghegh Ardabili, Ardabil, Iran
الکلمات المفتاحية: bioactive peptides, black cumin seed meal, broiler chickens, performance,
ملخص المقالة :
This study investigated the effects of hydrolyzed black cumin seed protein (HBCP) as a potential source of bioactive peptides on broiler chicken performance. A total of 560 Ross 308 broiler chicks were randomly allocated to seven dietary treatments: a control diet and six treatments containing varying levels of HBCP (0.05%, 0.1%, 0.15%, 0.2%), prebiotic (0.2%), or organic acid (0.2%). Results showed that the inclusion of 0.1%, 0.15%, and 0.2% HBCM in broiler diets improved body weight gain and feed conversion ratio during the entire rearing period. Additionally, the addition of 0.2% prebiotic and organic acid to the diet signifi-cantly increased body weight and decreased feed conversion ratio compared to the control group (P<0.05). Inclusion of 0.1%, 0.15%, and 0.2% HBCM in the diet significantly increased the digestibility of dry matter and organic matter compared to the control group (P<0.05). The use of 0.15% and 0.2% HBCM improved ash digestibility compared to the control group (P<0.05). The inclusion of prebiotics and organic acids in broiler diets also significantly improved the digestibility of dry matter, ash, and organic matter (P<0.05). Furthermore, the addition of HBCP, prebiotic, and organic acid positively influenced intestinal morphology, characterized by increased villus height, width, while decreasing crypt depth (P<0.05). Regarding blood biochemistry, HBCP, prebiotic, and organic acid supplementation led to decreased cholesterol, low-density lipoprotein (LDL), and triglyceride levels, while increasing high-density lipoprotein (HDL) levels (P<0.05). The results of this experiment demonstrated that bioactive peptides derived from black cumin hydrolysate exerted effects comparable to those of prebiotics and organic acids, improving productive traits in broilers.
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