Unveiling the potential antidiabetic, antidyslipidemic, and antimicrobial effects of methanolic root extracts of Nymphaea pubescens Willd. plant: Evidence from in-vitro and in-vivo approaches
الموضوعات :
Sahid Ahmad Santo
1
,
Tanmay Deb
2
,
Md. Abdus Salam
3
,
Arafat Miah
4
,
Md. Saddam Hussain
5
,
Anamika Datta
6
,
Mohammad Nurul Amin
7
,
Mohammad Sarowar Uddin
8
,
Mohammad Safiqul Islam
9
,
Md. Shalahuddin Millat
10
1 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
2 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
3 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
4 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
5 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
6 - Department of Pharmacy, R. P. Shaha University, Narayanganj-1400, Bangladesh
7 - Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka-1230, Bangladesh
8 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
9 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
10 - Department of Pharmacy, Noakhali Science and Technology University, Noakhali-3814, Bangladesh
الکلمات المفتاحية: Antidiabetic, Antihyperlipidemic, Antimicrobial, In vitro, In vivo, Nymphaea pubescens Willd., Nymphaeaceae ,
ملخص المقالة :
This study investigated the therapeutic potential of methanolic root extracts of Nymphaea pubescens Willd. with a focus on their antidiabetic, antilipidemic, and antimicrobial properties. The methanolic root extracts of the N. pubescens at higher doses (500 mg/kg) exhibited a pronounced serum blood glucose-lowering activity (p < 0.001) and also demonstrated remarkable outcomes in body weight loss (p < 0.01), closely resembling the effects of the standard metformin. The same experiment displayed a noticeable reduction in low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) levels (p < 0.001), along with a notable elevation in high-density lipoprotein cholesterol (HDL-C) levels (p < 0.01). Furthermore, the plant extract at 500 mg/kg unveiled significant antimicrobial activities (p < 0.001) against both Staphylococcus aureus and Aspergillus niger and also exhibited significant (p < 0.01) inhibition of Escherichia coli. In summary, these findings highlight the multifaceted therapeutic potential of N. pubescens root extracts, warranting further investigation for their possible development into novel therapeutic agents.
Acharjee, M., Zerin, N., Ishma, T., Mahmud, M.R. (2023) In-vitro anti-bacterial activity medicinal plants against urinary tract infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics. New Microbes New Infect. 51:1-11.
DOI: https://doi.org/10.1016/j.nmni.2022.101076.
Akinjogunla, O., Adegoke, A., Udokang, I., Adebayo-Tayo, B. (2009) Antimicrobial potential of Nymphaea lotus (Nymphaeaceae) against wound pathogens. J. Med. Plant Res. 3(3):138-141.
Akinyemi, K.O., Oladapo, O., Okwara, C.E., Ibe, C.C., Fasure, K.A. (2005) Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity. BMC Complement. Altern. Med. 5:1-7.
DOI: https://doi.org/10.1186/1472-6882-5-6.
Al-Ishaq, R.K., Abotaleb, M., Kubatka, P., Kajo, K., Büsselberg, D. (2019) Flavonoids and their anti-diabetic effects: Cellular mechanisms and effects to improve blood sugar levels. Biomolecules. 9(430):1-35.
DOI: https://doi.org/10.3390/biom9090430.
Angadi, K.K., Gundampati, R.K., Jagannadham, M.V., Kandru, A. (2013) Molecular docking studies of guggultetrol from Nymphaea pubescens with target glucokinase (GK) related to type-II diabetes. J. Appl. Pharm. Sci. 3(2):127-131.
DOI: https://doi.org/10.7324/JAPS.2013.30222.
Angadi, K.K., Kandru, A., Rahaman, A., (2013) Antihyperglycaemic, antihyperlipidaemic and antioxidant assays (in vivo) of Nymphaea pubescens leaf extract. Int. J. Pharma. Bio. Sci. 4(2):(B) 624-630.
Artha, I.M.J.R., Bhargah, A., Dharmawan, N.K., Pande, U.W., Triyana, K.A., Mahariski, P.A., Yuwono, J., Bhargah, V., Prabawa, I.P.Y., Manuaba, I.B.A.P. (2019) High level of individual lipid profile and lipid ratio as a predictive marker of poor glycemic control in type-2 diabetes mellitus. Vasc. Health Risk Manag. 15:49-157.
DOI: https://doi.org/10.2147/VHRM.S209830.
Arya, A.K., Durgapal, M., Bachheti, A., Joshi, K.K., Gonfa, Y.H., Bachheti, R.K., Husen, A. (2022) Ethnomedicinal use, phytochemistry, and other potential application of aquatic and semiaquatic medicinal plants. Evid.-Based Complementary Altern. Med. 2022:1-19.
DOI: https://doi.org/10.1155/2022/4931556.
Auwal, M.S., Saka, S., Mairiga, I.A., Sanda, K.A., Shuaibu, A., Ibrahim, A. (2014) Preliminary phytochemical and elemental analysis of aqueous and fractionated pod extracts of Acacia nilotica (Thorn mimosa). Vet. Res. Forum. 5(2):95-100.
Banso, A., Adeyemo, S. (2006) Phytochemical screening and antimicrobial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri. 18(1):39-44.
DOI: https://doi.org/10.4314/biokem.v18i1.56390.
Bassalat, N., Kadan, S., Melamed, S., Yaron, T., Tietel, Z., Karam, D., Kmail, A., Masalha, M., Zaid, H. (2023) In vivo and in vitro antidiabetic efficacy of aqueous and methanolic extracts of Orthosiphon stamineus Benth. Pharmaceutics. 15(3):1-14.
DOI: https://doi.org/10.3390/pharmaceutics15030945.
Bouhrim, M., Daoudi, N.E., Ouassou, H., Benoutman, A., Loukili, E.H., Ziyyat, A., Mekhfi, H., Legssyer, A., Aziz, M., Bnouham, M. (2020) Phenolic content and antioxidant, antihyperlipidemic, and antidiabetogenic effects of Opuntia dillenii seed oil. Sci. World J. 2020:1-8.
DOI: https://doi.org/10.1155/2020/5717052.
Calheiros, D., Dias, M.I., Calhelha, R.C., Barros, L., Ferreira, I.C., Fernandes, C., Gonçalves, T. (2023) Antifungal activity of spent coffee ground extracts. Microorganisms. 11(242):1-20.
DOI: https://doi.org/10.3390/microorganisms11020242.
Chehade, J.M., Gladysz, M., Mooradian, A.D. (2013) Dyslipidemia in type 2 diabetes: Prevalence, pathophysiology, and management. Drugs. 73:327-339.
DOI: https://doi.org/10.1007/s40265-013-0023-5.
Chukiatsiri, S., Wongsrangsap, N., Ratanabunyong, S., Choowongkomon, K. (2023) In vitro evaluation of antidiabetic potential of Cleistocalyx nervosum var. paniala fruit extract. Plants. 12(112):1-13.
DOI: https://doi.org/10.3390/plants12010112.
Cushnie, T.P.T., Lamb, A.J. (2005) Antimicrobial activity of flavonoids. Int. J. Antimicrob. Agents. 26(5):343-356.
DOI: https://doi.org/10.1016/j.ijantimicag.2005.09.002.
Dodamani, S., Sanakal, R., Kaliwal, B. (2012) Antidiabetic efficacy of ethanolic leaf extract of Nymphaea odorata in alloxan induced diabetic mice. Int. J. Pharm. Pharm. Sci. 4:338-341.
Fisher, M.C., Gurr, S.J., Cuomo, C.A., Blehert, D.S., Jin, H., Stukenbrock, E.H., Stajich, J.E., Kahmann, R., Boone, C., Denning, D.W. (2020) Threats posed by the fungal kingdom to humans, wildlife, and agriculture. MBio. 11(3):e00449-20.
DOI: https://doi.org/10.1128/mbio.00449-20.
Gueye, F.K., Niang, L., Dieng, B., Gueye, M.F., Ayessou, N.C., Mbaye, M.S., Noba, K. (2022) Phytochemical screening and antioxidant activities of water lilies seeds neglected and underused species in the delta and lower valley of the Senegal river. Am. J. Plant Sci. 13(6):756-765.
DOI: https://doi.org/10.4236/ajps.2022.136051.
Hossain, M.M., Uddin, M.S., Baral, P.K., Ferdus, M., Bhowmik, S. (2022) Phytochemical screening and antioxidant activity of Ipomoea hederifolia stems: A potential medicinal plant. Asian J. Nat. Prod. Biochem.. 20(2):41-47.
DOI: https://doi.org/10.13057/biofar/f200201.
Hui, Z., Zhou, X., Li, R., Bin Wang, Y., Ma, J. (2015) Studies on the extraction process of total flavonoids in Radix puerariae and their hypoglycemic effect in mice. Biomed. Res. 26(1):51-54.
Hussain, M.S., Jahan, N., Rashid, M.M.O., Hossain, M.S., Chen, U., Rahman, N. (2019) Antihyperlipidemic screening and plasma uric acid reducing potential of Momordica charantia seeds on Swiss albino mice model. Heliyon. 5(5):e01739.
DOI: https://doi.org/10.1016/j.heliyon.2019.e01739.
Ifesan, B., Fashakin, J., Ebosele, F., Oyerinde, A. (2013) Antioxidant and antimicrobial properties of selected plant leaves. European J. Med. Plants. 3(3):465-473.
DOI: https://doi.org/10.5555/20133287109.
Kancherla, N., Dhakshinamoothi, A., Chitra, K., Komaram, R.B. (2019) Preliminary analysis of phytoconstituents and evaluation of anthelminthic property of Cayratia auriculata (in vitro). Maedica. 14(4):350-356.
DOI: https://doi.org/10.26574/maedica.2019.14.4.350.
Marzan, S., Bulbul, L., Halim, M.A., Alam, M.Z. (2023) In-vivo assessment of hypoglycemic and anxiolytic activities on Swiss albino mice and anthelmintic activity on earthworm of methanolic extract of Microcos paniculata leaves. EPRA IJRD. 8(3):91-96.
DOI: https://doi.org/10.53365/nrfhh/177626.
Mazumder, T., Mamun, I.P., Zaman, M.S., Islam, A.K., Chowdhury., S., Reza, M.S., Hussain, M.S. (2021) Comparative lipid and uric acid suppressing properties of four common herbs in high fat-induced obese mice with their total phenolic and flavonoid index. Biochem Biophys. Rep. 26:1-8.
DOI: https://doi.org/10.1016/j.bbrep.2021.100990.
Mokha, J.S., Srinivasan, S.R., DasMahapatra, P., Fernandez, C., Chen, W., Xu, J. (2010) Berenson GS. utility of waist-to-height ratio in assessing the status of central obesity and related cardiometabolic risk profile among normal weight and overweight/obese children: The Bogalusa Heart Study. BMC Pediatr. 10:1-7.
DOI: https://doi.org/10.1186/1471-2431-10-73.
Organization, W.H. (2019) New report calls for urgent action to avert antimicrobial resistance crisis. Joint News Release. https://www.who.int/news/item/29-04-2019-new-report-calls-for-urgent-action-to-avert-antimicrobial-resistance-crisis.
Pokhrel, T., Shrestha, D., Dhakal, K., Yadav, P.M., Adhikari, A. (2022) Comparative analysis of the antioxidant and antidiabetic potential of Nelumbo nucifera Gaertn. and Nymphaea lotus L. var. pubescens (Willd.). J. Chem. 2022:1-5.
DOI: https://doi.org/10.1155/2022/4258124.
Prodhan, A., Mridu, F.S. (2023) Antidiabetic properties of nymphaea species (water lilies): A review. Nat. Prod. J. 13(1):78-112.
DOI: https://doi.org/10.2174/2210315512666220405083207.
Raja, M.M.M., Sethiya, N.K., Mishra, S. (2010) A comprehensive review on Nymphaea stellata: a traditionally used bitter. J. Adv. Pharm. Technol. Res. 1(3):311-319.
DOI: https://doi.org/10.4103/0110-5558.72424.
Rajagopal, K., Sasikala, K. (2008) Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan-induced diabetic rats. Singap. Med. J. 49(2):137-141.
Salam, M.A., Rana, M.F., Baral, P.K., Rahman, M.O., Ahmed, S.R.R., Jahan, N., Mazumder, T., Islam, M.S., Hussain, M.S. (2023) Glipizide has low influences on lipid index and major organs weight variation and considerable anxiolytic properties: An in vivo Investigation. J. Med. Sci. 23:7-17.
DOI: https://doi.org/10.3923/jms.2023.7.17.
Selvakumari, E., Shantha, A., Kumar, C.S., Prabhu, T.P. (2016) Phytochemistry and pharmacology of the genus Nymphaea. J. Acad. Ind. Res. 5(7):98-108.
Shajeela, P.S., Kalpanadevi, V., Mohan, V.R. (2012) Potential antidiabetic, hypolipidaemic and antioxidant effects of Nymphaea pubescens extract in alloxan induced diabetic rats. J. Appl. Pharm. Sci. 02(02):83-88.
DOI: https://doi.org/10.5555/20123336960.
Shams-Ghahfarokhi, M., Shokoohamiri, M-R., Amirrajab, N., Moghadasi, B., Ghajari, A., Zeini, F., Sadeghi, G., Razzaghi-Abyaneh, M. (2006) In vitro antifungal activities of Allium cepa, Allium sativum and ketoconazole against some pathogenic yeasts and dermatophytes. Fitoterapia. 77(4):321-323.
DOI: https://doi.org/10.1016/j.fitote.2006.03.014.
Sharmin, T., Sultana, R., Hossain, F., Shakil, S.K., Hossen, F., Rashid, M.M.O. (2018) Neuropharmacological and antibacterial effects of the ethyl acetate extract of Diospyros malabarica (Ebenaceae) seeds. Clin. phytosci. 4:1-8.
DOI: https://doi.org/10.1186/s40816-018-0083-x.
Sundaram, T., Napagoda, M., Wijesekera, K., Wijayaratne, G.B. (2020) Determination of Total Phenolic Content (TPC) of Nymphaea nouchali and Nymphaea pubescens by Folin-Ciocalteu Method. International Conference on Frontiers in Chemical Technology. IchemC, pp. 58.
Taskinen, M.R. (2002) Diabetic dyslipidemia. Atheroscler. Suppl. 3(1):47-51.
DOI: https://doi.org/10.1016/S1567-5688(01)00006-X.
Thawabteh, A., Juma, S., Bader, M., Karaman, D., Scrano, L., Bufo, S.A., Karaman, R. (2019) The biological activity of natural alkaloids against herbivores, cancerous cells and pathogens. Toxins. 11(656):1-28.
DOI: https://doi.org/10.3390/toxins11110656.
Vinayagam, R., Jayachandran, M., Xu, B. (2016) Antidiabetic effects of simple phenolic acids: A comprehensive review. Phytother. Res. 30(2):184-199.
DOI: https://doi.org/10.1002/ptr.5528.
