In vitro Gas Production and Fermentation Parameters of Some Plants Species Collected from Algerian Arid Rangelands

الموضوعات :

Souhil Boufennara ¹ , Samir Medjekal ² , Lyas Bouazza ³ , Amal Hamedellou ⁴ , Ibtissem Bella ⁵ , Nour Elhouda Ayeb ⁶ , Secunino Lopez ⁷

1 - Department of Cellular and Molecular Biology, Faculty of Nature Sciences, University Abbès Laghrour of Khenchela, 40000. Khenchela, Algeria
2 - Department of Microbiology and Biochemistry, Faculty of Science, University Mohamed Boudiaf of M'sila, Algeria
3 - Department of Cellular and Molecular Biology, Faculty of Nature Sciences, University Abbès Laghrour of Khenchela, 40000. Khenchela, Algeria
4 - Faculty of nature Sciences, University Abbès Laghrour of Khenchela, 40000. Khenchela, Algeria
5 - Faculty of nature Sciences, University Abbès Laghrour of Khenchela, 40000. Khenchela, Algeria
6 - Faculty of nature Sciences, University Abbès Laghrour of Khenchela, 40000. Khenchela, Algeria
7 - Institute of Livestock of Mountain -CSIC- Department of Animal Production, University of Léon, 24007 León, Spain

تاريخ الإرسال : 08 السبت , رجب, 1442 تاريخ التأكيد : 04 الأحد , شوال, 1442 تاريخ الإصدار : 28 السبت , جمادى الأولى, 1443

الکلمات المفتاحية: gas production, rumen, nutritive value, volatile fatty acids, forages,

ملخص المقالة :

The objective of the study was to evaluate the nutritional value of some native plants, collected from Algerian arid zones by in vitro gas production method. The volatile fatty acids (VFA) of samples were also determined in the culture medium. This work was carried out in 2011 and was conducted in comparison to a control substrate (oat vetch) considered as a reference plant in literature. The selected plants were collected in the arid areas of the Algerian desert. Nine forages including six dicotyledon plants (Atriplex halimus L., Artemisia campestris L., Artemisia herba-alba subsp. valentina, Astragalus gombiformis Pomel, Spartidium saharae (Coss. & Dur.) and Retama raetam Forssk., and three monocotyledon plants (Stipagrostis pungens (Desf.), Lygeum spartum L.and Stipa tenacissima L.) were selected. A. campestris, A. gombiformis and A. herba-alba recorded the highest values of gas production. The total VFA production of the different substrates is significantly different between them (p<0.0001). A. gombiformis had the highest total VFA (34.7 mmol/L) followed by A. campestris (32.8 mmol/L), while the lowest total production of VFA was observed in S. tenacissima (17.3 mmol/L). Generally, the plant studied can be classified in two groups, one group with poor-quality grasses (L. spartum. and S. tenacissima) and other with higher digestibility (A. gombiformis and Artemisia spp.). In conclusion, dicot species are therefore recommended for feeding ruminants.

المصادر:

Andrighetto, I., Gruber, L., Cozzi, G., Uray, G., Guidetti, G., Buchgraber, K., 1992. Prediction of digestible organic matter in dry matter in vivo from the chemical composition, in vitro and in situ measurements on native mountain forages. Anim. Feed Sci. Technol., 39(3-4), 323-333.

Aregheore, E.M., 2000. Chemical composition and nutritive value of some tropical by-product feedstuffs for small ruminants - In vivo and in vitro digestibility. Anim. Feed Sci. Technol., 85(1-2), 99-109.

Arhab, R., 2006. Etude de la fermentescibilité in vitro et de la digestibilité in vivo de végétaux des zones arides et de sous-produits de l’agronomie saharienne par l’estimation de l’activité métabolique du microbiote ruminal d’ovins. Thèse de de doctorat. Université Mentouri de Constantine, Algeria.

Ballet, N., 1989. Contribution à l’étude cinétique de la lignification et à l’étude de l’action d’un inhibiteur de ce processus chez le blé et le maïs. Mesures obtenues par voies biochimiques et histologiques D.E.A. Université Blaise-Pascal, Clermont-Ferrand, France.

Beuvink, J.M.W., and Spoelstra, S.F., 1992. Interactions between substrate, fermentation end-products, buffering systems and gas production upon fermentation of different carbohydrates by mixed rumen microorganisms in vitro. Appl. Microbiol. Biotechnol., 37(4),505-509.

Blümmel, M., Aiple, K.P., Steingass, H., Becker, K. A., 1999. Note on the stoichiometrical relationship of short chain fatty acid production and gas formation in vitro in feedstuffs of widely differing quality. J. Anim. Physiol. Nutr., 81(3), 157-167.

Bouazza, L., Boufennara, S., Bensaada, M., Zeraib, A., Rahal, K., 2019. In vitro screening of Algerian steppe browses plants for digestibility, rumen fermentation profile and methane mitigation. Agroforestry Systems., 94, 1433- 1443.

Bouazza, L., Lopez, S., Boufennara, S., Bousseboua, H., Bodas, R., 2012. Nutritive evaluation of foliage from fodder trees and shrubs characteristic of Algerian arid and semi-arid areas. J. of Anim. and Feed Sci., 21,521-536.

Boufennara, S., Bouazza, L., Medjekal, S., Rahal, K., Mateos, E., 2019. Comparing chemical composition and digestibility of pedicels and palm leaves as a source for livestock feeding by in vitro and in situ techniques. J. Rangel. Sci., 9 (4), 378-391.

Boufennara, S., Lopez, S., Bousseboua, H., Bodas, R., Bouazza, L., 2012. Chemical composition and digestibility of some browse plant species collected from Algerian arid rangelands. Spanish J. of Agric. Res., 10 (1), 88- 98.

Brown, V.E., Rymer, C., Agnew, R.E., Givens, D.I., 2002. Relationship between in vitro gas production profiles of forages and in vivo rumen fermentation patterns in beef steers fed those forages. Anim. Feed Sci. Technol., 98(1-2), 13-24.

Cabiddu, A., Decandia, M., Sitzia, M., Molle, G., 2000. A note on the chemical composition and tannin content of some Mediterranean shrubs browsed by Sarda goats. Cahiers Options Méditerranéennes., 52, 175-178.

De Peters, E.J., Fadel, J.G., Arana, M.J., Ohanesian, N, Etchebarne, M.A., 2000. Variability in the Chemical Composition of Seventeen Selected By-Product Feedstuffs Used by the California Dairy Industry. The Professional Animal Scientist., 16(2), 69-99.

Garcıa-Gonzalez, R., Lopez, S., Fernandez, M., Bodas, R., Gonzalez, J.S., 2008. Screening the activity of plants and spices for decreasing ruminal methane production in vitro. Anim. Feed Sci. Technol., 147, 36–52.

Getachew, G., Blümmel, M., Makkar, H.P.S., Becker, K., 1998. In vitro gas measuring techniques for assessment of nutritional quality of feeds: A review. Anim. Feed Sci. Technol., 72(3-4), 261-281.

Getachew, G., Crovetto, G.M., Fondevila, M., Krishnamoorthy, U., Singh, B., 2002. Laboratory variation of 24 h in vitro gas production and estimated metabolizable energy values of ruminant feeds. Anim. Feed Sci. Technol., 102(1-4), 169-180.

Getachew, G., Robinson, P.H., DePeters, E.J., Taylor, S.J., 2004. Relationships between chemical composition, dry matter degradation and in vitro gas production of several ruminant feeds. Anim. Feed Sci. Techno., 111(1-4), 57-71.

Gokkuş, A., Koc, A., 2001. Range Management. Ataturk University,Yakutiye, Turkey.

Gulşen, N., Coşkun, B., Umucalilar, H.D., Dural, H., 2004. Prediction of nutritive value of a native forage, using of in situ and in vitro measurements. J. Arid Environ., 56(1), 167-179.

Hoover, W.H., 1986. Chemical Factors Involved in Ruminal Fiber Digestion. J. Dairy Sci., 69 (10), 2755-2766.

Jouany, J.P., 1982. Dosage des acides gras volatils dans les contenus digestifs, les jus d’ensilage, les cultures bactériennes et les contenus de fermenteurs anaérobies. Science Aliment., 2, 131-144.

Khazaal, K., Dentinho, M.T., Ribeiro, J.M., Ørskov, E.R., 1993. A comparison of gas production during incubation with rumen contents in vitro and nylon bag degradability as predictors of the apparent digestibility in vivo and the voluntary intake of hays. Anim. Sci., 57(01), 105-112.

Khazaal, K., Dentinho, M.T., Ribeiro, J.M., Ørskov, E.R., 1995. Prediction of apparent digestibility and voluntary intake of hays fed to sheep: Comparison between using fibre components, in vitro digestibility or characteristics of gas production or nylon bag degradation. Anim. Sci., 61(03), 527-538.

Krishnamoorthy, U., Soller, H., Steingass, H., Menke, K.H., 1995. Energy and protein evaluation of tropical feedstuffs for whole tract and ruminal digestion by chemical analyses and rumen inoculum studies in vitro. Anim. Feed Sci. Technol., 52(3-4), 177-188.

Larbi, A., Smith, J.W., Kurdi, I.O., Adekunle, I.O., Raji, AM., 1998.Chemical composition, rumen degradation, and gas production characteristics of some multipurpose fodder trees and shrubs during wet and dry seasons in the humid tropics. Anim. Feed Sci. Technol., 72(1-2), 81-96.

Longuo, H.F., Chehma, A., OuledBelkher, A., 1989. Quelques aspects botaniques et nutritionnels des pâturages des dromadaires en Algérie. In : Séminaire sur la digestion, la nutrition et l’alimentation des dromadaires. Cahiers Options Méditerranéennes., 2,101-113.

Lopez, S., Dhanoa M.S., Dijkstra, J., Bannink, A., Kebreab, E., France,J., 2007. ‘Some methodological and analytical considerations regarding application of the gas production technique. Anim., Feed Sci. and Techno., 135, 139–156

Mayouf, R., and Arbouche, F., 2014. Chemical composition and relative feed value of three Mediterranean fodder shrubs. Afri. J. of Agri. Rese., 746-749

McDonald, P., Greenhalgh, J.F.D., Edwards, R.A., Morgan, C.A., 1995. Animal nutrition. 5th, Longmanns, London.

Medjekal, S., Ghadbane, M., Bodas, R., Bousseboua, H., López, S., 2018. Volatile fatty acids and methane production from browse species of Algerian arid and semi-arid areas, J. Appl. Anim. Res., 46 (1), 44-49.

Menke, K.H. and Steingass, H., 1988. Estimation of the energetic feed value obtained from
chemical analyses and gas production using rumen fluid. Anim. Res. Dev., 28, 7-55.

Nedjraoui, D., 2001.Algeria Country pasture. Forage Resource Profiles, F.A.O. info

Orskov, E., R., and McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weigheted according to rate of passage. J. Agri. Sci. C., 92, 499-503.

Papanastasis, V.P., Yiakoulaki, M.D., Decandia, M., Dini-Papanastasi, O., 2008. Integrating woody species into livestock feeding in the Mediterranean areas of Europe. Anim. Feed Sci. Technol., 140 (1-2), 1-17.

SAS INSTITUTE INC. SAS/STAT® 9.2., 2008.User's Guide.SAS Institute Inc., Cary, NC, USA.

Steel, R.G.D., Torrie, J.H., 1980. Principles and Procedures of Statistics: A biometrical approach, 2nd Edition, McGraw-Hill Book Company, New York.

Theodorou, MK, Williams, BA, Dhanoa, MS, McAllan, AB, France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim Feed Sci Technol., 48(3-4), 185-197.

Tilley, J.M.A., Terry, R.A., 1963. A two-stage technique for the in vitro digestion of forage crops. J Bri Grass Soci., 18, 104-111.

Wolin, M.J., 1975. Interaction between the bacterial species in the rumen. In: Digestion and Metabolism in the Ruminant, Mc Donald J.W. and Warner A.C.I. (Eds.), Armidale, Australia.