Fenugreek Seed (Trigonella foenum-graecum) and Asparagus Root (Asparagus officinalis) Effects on Digestion and Kinetics of Gas Production of Alfalfa Hay Using in vitro Technique
الموضوعات :و. ناصری 1 , ف. کفیلزاده 2 , ف. هژبری 3
1 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermakshah, Iran
2 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermakshah, Iran
3 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermakshah, Iran
الکلمات المفتاحية: digestibility, alfalfa, gas production, asparagus root, fenugreek seed, <, i>, in vitro<, /i>, ,
ملخص المقالة :
The aim of this paper was to study of the effect of fenugreek seed (Trigonella foenum-graecum) and asparagus root (Asparagus officinalis) on in vitro digestibility and kinetics of gas production of alfalfa hay. Fenugreek seed (FS) and asparagus root (AR) were added at different levels (0, 5, 10, 15 and 20% of DM). Total phenolic components of alfalfa hay (AH), fenugreek seed (FS) and asparagus root (AR) were 5.9, 10 and 8.3 g/kg DM, total tannins 0.4, 3.8 and 1.5 g/kg DM and saponin 10.4, 27.3 and 40.3 g/kg DM, respectively. In vitro dry matter (DM), organic matter (OM) and neutral detergent fiber (NDF) digestibility of alfalfa decreased (P<0.05) by addition of different levels of FS, but were not affected by adding AR. Addition of FS did not affect alfalfa crude protein (CP) digestibility, but AR at levels of 5 and 20% DM decreased (P<0.05) it. Metabolizable energy (ME) increased (P<0.05) due to addition of FS, but it decreased (P<0.05) by incorporation of AR. Addition of FS (at 10% DM level) and AR (at 5% DM level) decreased (P<0.05) potential gas production. The rate constants (c and d) were not affected by addition of FS and AR. FS and AR at level of 5% DM decreased (P<0.05) lag time (l). FS did not affect fermentation rate (h-1), but AR at level of 10% DM increased (P<0.05) it. Results suggest that fenugreek seed and asparagus root due to secondary metabolites content's may have potential to improve the nutritive value of alfalfa hay
Alexander G., Singh B., Sahoo A. and Bhat T.K. (2007). In vitro screening of plant extracts to enhance the efficiency of utilization of energy and nitrogen in ruminant diets. Anim. Feed Sci. Technol. 145, 229-242.
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Baccou J.C., Lambert F. and Sauvaire Y. (1977). Spectrophotometric method for determination of total steroidal sapogenin. Analyst. 102, 458-465.
Blümmel M. and Becker K. (1997). The degradability characteristics of 54 roughages and roughage neutral detergent fiber as described by in vitro gas production and their relationship to voluntary feed intake. Br. J. Nutr. 77, 757-786.
ChurchD.C. and Pond W.G. (1988). Basic Animal Nutrition and Feeding. J. Wiley and S. Inc, Ed. New York, USA.
Duncan D.B. (1955). Multiple range and multiple F-tests. Biometrics. 11, 1-42.
France J., Dijkstra J., Dhanoa M.S., Theodorou M.K., Lister S.J., Davies D.R. and Isac D.A. (1993). A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. J. Theoret. Biol. 163, 99-111.
Getachew G., Blummel M., Makkar H.P.S. and Becker K. (1998). In vitro gas measuring techniques for assessment ¨ of nutritional quality of feeds: a review. Anim. Feed Sci. Technol. 72, 261-281.
Getachew G., Depeters E.J. and Robinson P.H. (2004). In vitro gas production provides effective method for assessing ruminant feeds. Cal. Agric. 58(1), 54-58.
Julkunen-Titto R. (1985). Phenolics constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J. Agric. Food Chem. 33, 213-217.
Lopez S., Dhanoa M.S., Dijkstra J., Bannink A., Kebreab E. and France J. (2007). Some methodological and analytical considerations regarding application of the gas production technique. Anim. Feed Sci. Technol. 135, 139-156.
Makkar H.P.S., Blummel M. and Becker K. (1995). In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. J. Sci. Food Agric. 69, 481-493.
Makkar H.P.S. (2004). Recent advances in the in vitro gas method for evaluation of nutritional quality of feed resources. Pp. 55-88 in Assessing Quality and Safety of Animal Feeds. FAO Animal Production and Health Series. Rome.
Makkar H.P.S. (2005). In vitro gas methods for evaluation of feeds containing phytochemicals. Anim. Feed Sci. Technol. 123, 291-302.
Menke K.H. and Steingass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 7-55.
Mirzaei-Aghsaghali A., Maheri-Sis N., Mansouri H., Razeghi ME., Shayegh J. and Aghajanzadeh-Golshani A. (2011). Evaluating nutritional value of apple pomace for ruminants using in vitro gas production technique. Ann. Biol. Res. 2, 100-106.
Naseri V., Hozhabri F. and Kafilzadeh F. (2012). Assessment of in vitro digestibility and fermentation parameters of alfalfa hay based diet following direct incorporation of fenugreek seed (Trigonella foenum) and asparagus root (Asparagus officinalis). J. Anim. Physiol. Anim. Nutr.97(4), 773-784.
Newbold C.J., Lassalas B. and Jouany J.P. (1995). The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Lett. Appl. Microbiol. 21, 230-234.
Pell A.N., Mackie R.I., Mueller-Harvey I. and Ndlovu L.R. (2001). Tannins: analysis and biological effects in ruminant feed (special issue). Anim. Feed Sci. Technol. 91(1), 1-113.
SAS Institute. (1996). SAS®/STAT Software, Release 6.11. SAS Institute, Inc., Cary, NC. USA.
Scehovic J. (1999). Evaluation in vitro de l’activité de la population microbienne du rumen en présence d’extraits végétaux. Rev. Suisse. Agric. 31, 89-93.
Sliwinski B.J., Soliva C.R., Machmuller A. and Kreuzer M. (2002). Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Anim. Feed Sci. Technol. 101, 101-104.
Stumm C.K. and Zwart K.B. (1986). Symbiosis of protozoa with hydrogen utilizing methanogens. Microbial. Sci. 3, 100-105.
Theodorou M.K., Williams B.A., Dhanoa M.S., McAllan A.B. and France J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Technol. 48, 185-197.
Tilley J.M.A. and Terry R.A. (1963). A two-stage technique for the in vitro digestion of forage crops. J. Br. Grassl. Soc. 18, 2.
Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Carbohydrate methodology, metabolism and nutritional implications in dairy cattle. J. Dairy Sci.74, 3583-3597.
Wang Y., McAllister T.A., Yanke L.J. and Cheek P.R. (2000). Effect of steroidaul saponin from Yucca schidigera extract on ruminant microbes. J. Appl. Microbiol. 88, 887-896.
