Association between Ovoinhibitor (OIH) Gene Polymorphism and Egg Quality Traits in Golden Kamper Hybrid Chickens (Gallus gallus domesticus)
الموضوعات :
D. Retnosari
1
,
B.S. Daryono
2
1 - Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, 55281, Indonesia
2 - Genetics and Breeding Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan Sekip Utara, 55281, Indonesia
الکلمات المفتاحية: chicken, polymorphism, egg quality, ovoinhibitor gene,
ملخص المقالة :
The ovoinhibitor (OIH) has been identified in all egg compartments including egg white, egg yolk, and eggshell. The objective of this study was to analyze the association between OIH gene polymorphism and egg quality in the Golden Kamper Hybrid chickens. The chickens used in this study were BC Golden Kamper and F4 Golden Kamper. Chickens were maintained in the individual cage to observe egg quality. Chicken blood samples were taken for DNA isolation by Chelex 5% method followed by OIH gene amplification and polymorphism detection with sanger sequencing. Significant differences in egg quality were analyzed using one-way ANOVA. The relationship between genotype and haplotype with egg quality was analyzed using Pearson correlation. Average egg weight, egg length, shell weight, and shell thickness of BC Golden Kamper were 47.06 ± 2.91 g, 5.13 ± 0.3 mm, 4.58 ± 0.52 g, 0.30 ± 0.03 mm and F4 Golden Kamper was 47.42 ± 3.74 g, 5.16 ± 0.2 mm, 5.18 ± 0.33 g and 0.315 ± 00 mm. The mean albumen height, haugh unit, and yolk color of BC Golden Kamper was 5.4 ± 1.14 mm, 85.86 ± 8.71, 8.80 ± 0.45 and F4 Golden Kamper was 6.13 ± 0.33 mm, 79.42 ± 2.21, 8.00 ± 0. There was a single nucleotide polymorphisms (SNP) G4363T in the OIH gene intron 7. Genotype (GG and TT) and haplotype (H1 and H2) were significantly negatively correlated with albumen weight (P<0.05), shell weight (P<0.05), and shell thickness (P<0.05).
Aberra M., Zemen W. and Yosef T.G. (2012). Assessment of the prevailing handling and quality of eggs from scavenging indigenous chickens reared in different agro-ecological zone of Ethiopia. Res. J. Poult. Sci. 5(4), 64-70.
Bhattacharya T.K., Chatterjee R.N., Dange M. and Bhanja S.K. (2019). Polymorphisms in GnRHI and GnRHII genes and their association with egg production and egg quality traits in chicken. British Poult. Sci. 60(3), 187-194.
Bourin M., Gautron J., Berges M., Attucci S., Le Blay G., Labas V., Nys Y. and Rehault-Godbert S. (2011). Antimicrobial potential of egg yolk ovoinhibitor, a multidomain kazal-like inhibitor of chicken egg. J. Agric. Food Chem. 59, 12368-12374.
Eddin A.S., Ibrahim S.A. and Tahergorabi R. (2019). Egg quality and safety with an overview of edible coating application for egg preservation. Food Chem. 296, 29-39.
Gao D., Qiu N., Liu Y. and Ma M. (2017). Comparative proteome analysis of egg yolk plasma proteins during storage. J. Sci. Food Agric. 97, 2392-2400.
Hanusová E., Hrnčár C., Hanus A. and Oravcová M. (2015). Effect of breed on some parameters of egg quality in laying hens. Acta Fytotechnol Zootechnol. 18, 20-24.
Huang T., Ma J., Gong Y. and Feng Y. (2019). Polymorphisms in the ovoinhibitor gene (OIH) and their association with egg quality of Xinhua E-strain chickens. British Poult. Sci. 60(2), 88-93.
Isidahomen C.E., Njidda A.A. and Olatunji E.A. (2013). Egg quality traits of indigenous and exotic chickens as influenced by specific genes. J. Biol. Agric. Health. 3(1), 53-58.
Karve R., Liu W., Willet S.G., Torii K.U. and Shpak E.D. (2011). The presence of multiple introns is essential for ERECTA expression in arabidopsis. RNA. 17(10), 1907-1921.
Kinoshita K., Shimogiri T., Okamoto S., Yoshizawa K., Mannen H. and Ibrahim H.R. (2004). Linkage mapping of chicken ovoinhibitor and ovomucoid genes to chromosome 13. Anim. Genet. 35(4), 356-358.
Kostaman T. and Sopiyana S. (2016). The differences in egg quality of White Leghorn and naked neck chicken. Int. Semin. Livest. Prod. Vet. Technol. 2007, 365-369.
Li L., Li D., Liu L., Li S., Feng Y., Peng X. and Gong Y. (2015). Endothelin receptor B2 (EDNRB2) gene is associated with spot plumage pattern in domestic ducks (Anas Platyrhynchos). PLoS One. 10(5), e0125883.
Martos G., Lopez-Fandino R. and Molina E. (2013). Immunoreactivity of hen egg allergens: influence on in vitro gastrointestinal digestion of the presence of other egg white proteins and of egg yolk. Food Chem. 136(2), 775-781.
Myers R.L., Airey D.C., Manier D.H., Shelton R.C. and Sanders-Bush E. (2007). Polymorphisms in the regulatory region of the human serotonin 5-HT2A receptor gene (HTR2A) influence gene expression. Biol. Psych. 61(2), 167-173.
Nagpal K., Watanabe K.S.B., Tsao P. and Tsokos G.C. (2014). Transcription factor ikaros represses protein phosphatase 2A (PP2A) expression through an intronic binding site. J. Biol. Chem. 289(20), 13751-13757.
Padhi M.K., Chatterjee R.N., Haunshi S. and Rajkumar U. (2013). Effect of age on egg quality in chicken. Indian J. Poult. Sci. 48(1), 122-125.
Petibon C., Parenteau J., Catala M. and Elela S.A. (2016). Introns regulate the production of ribosomal proteins by modulating splicing of duplicated ribosomal protein genes. Nucleic Acids Res. 44(8), 3878-3891.
Saragih H.T.S.S.G. and Daryono B.S. (2012). Effect of high protein diet on body weight and pectoralis thoracicus muscle performance on pelung and broiler chicken (Gallus gallus domesticus). Anim. Prod. 14(3), 199-204.
Sayed M.A.M., Abouelezz F.M.K. and Abdel-Wahab A.A.M. (2017). Analysis of sperm motility, velocity and morphometry of three Egyptian indigenous chicken strains. Egyptian Poult. Sci. J. 37(4), 1173-1185.
Singh U.A., Kumari M. and Iyengar S. (2018). Method for improving the quality of genomic DNA obtained from minute quantities of tissue and blood samples using Chelex 100 resin. Biol. Proc. Online. 20, 12-21.
Słowińska M., Liszewska E., Nynca J., Bukowska J., Hejmej A., Bilińska B., Szubstarski J., Kozłowski K., Jankowski J. and Ciereszko A. (2014). Isolation and characterization of an ovoinhibitor, a multidomain kazal-like inhibitor from turkey (Meleagris gallopavo) seminal Plasma1. Biol. Reprod. 91, 108-117.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Yang W., Bei X., Liu M. and Qi X. (2015). Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression. Biochem. Biophys. Res. Commun. 463(4), 1097-1101.
