The Effect of Soybean Meal Heating Time on the in vitro Digestibility and Ruminal Fermentation Profile
الموضوعات :W. Wulandari 1 , B.P. Widyobroto 2 , C.T. Noviandi 3 , A. Agus 4
1 - Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
2 - Department of Animal Production, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
3 - Department of Animal Nutrition and Feed Science, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
4 - Department of Animal Production, Faculty of Animal Science, Gadjah Mada University, Yogyakarta, Indonesia
الکلمات المفتاحية: Degradation, rumen, soybean meal, protection, <i>in vitro</i>,
ملخص المقالة :
The aim of this study was to determine the most optimal heating time in protection of protein rich feedstuff on digestibility and in vitro ruminal fermentation profile. Proteinous feedstuffs used in this study is soybean meal (Glycine max). This study is designed using one way ANOVA, with five treatments of heating time (T0 (control)= unheated, T1= 10 min, T2= 20 min, T3= 30 min, and T4= 40 min) at 120 ˚C and 6 replications. All the treatment samples then incubated for 48 h according to the 2-stage in vitro technique. The results showed that protecting soybean meal through heating decreased the dry matter (DM), organic matter (OM) digestibility, NH3 concentration and acetic acid:propionate ratio (A:P) (p < 0.05) compared with the control group. In general, there were no significant effects on ruminal pH, total and proportion of volatile fatty acids (VFA), and microbial protein. A decrease in NH3 concentration and A:P ratio was seen in T2 (49.05 mg/100 mL and 1.52, respectively). It can be concluded that protein protection in soybean meal through heat treatment can decrease rumen degradation. The best heating time for protecting soybean meal was found at 20 minutes.
Agus A. (2012). Bahan Pakan Konsentrat untuk Sapi. PT Citra Aji Parama. Yogyakarta.
AOAC. (2005). Official Methods of Analysis. 12th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.
Banaszkiewicz T. (2011). Nutritional value of soybean meal. Pp. 1-20 in Soybean and Nutrition. H. El-Shemy, Ed. In-Tech Publishing, Rijeka, Croatia.
Boucher S.E., Calsamiglia S., Parsons C.M., Stein H., Stern M.D., Erickson P.S., Utterback P.L. and Schwab C.G. (2009). Intestinal digestibility of amino acids in rumen-undegraded protein estimated using a precision-fed cecectomized rooster bioassay: II. Distillers dried grains with solubles and fish meal. J. Dairy Sci. 92, 6056-6067.
Chaney A.L. and Marbach E.P. (1962). Modified reagent for determination urea and ammonia. Clin. Chem. 8, 130-132.
Cheeke P.R. (2005). Applied Animal Nutrition: Feed and Feeding. Pearson Prentice Hall, New Jersey, United States.
Chen K.J., Jan D.F., Chiou P.W.S. and Yang D.W. (2002). Effects of dietary heat extruded soybean meal and protected fat supplement on the production, blood and ruminal characteristics of holstein cows. Asian-Australasian J. Anim. Sci. 15(6), 821-827.
Dourado L.R.B., Pascoal L.A.F., Sakomura N.K., Costa F.G.P. and Biagiotti D. (2011). Soybeans (Glycine max) and soybean products in poultry and swine nutrition. Pp. 175-190 in Recent Trends for Enhancing the Diversity and Quality of Soybean Products. D. Krezhova, Ed. In-Tech Publishing, Rijeka, Croatia.
El-Waziry A.M., Nasser M.E.A., Sallam S.M.A., Abdallah A.L. and Bueno I.C.S. (2007). Effect of autoclaving and quebracho tannin treated- soybean meal on gas production and rumen fermentation in vitro. J. Appl. Sci. Res. 3(1), 17-24.
Filipek J. and Dvorak R. (2009). Determination of the volatile fatty acid content in the rumen liquid: Comparison of gas chromatography and capillary isotachophoresis. Acta Vet. Brno. 78, 627-633.
Giallongo F., Oh J., Frederick T., Isenberg B., Kniffen D.M., Fabin R.A. and Hristov A.N. (2015). Extruded soybean meal increased feed intake and milk production in dairy cows. J. Dairy Sci. 98(9), 1-15.
Haraki H.M.C., Gandra J.R., Oliveira E.R., Takiya C.S., Goes R.H.T.B., Gabriel A.M.A., Rodrigues G.C.G., Gandra E.R.S., Pereira T.L., Damiani J. and Batista J.D.O. (2018). Effects of chitosan and whole raw soybeans on feeding behavior and heat losses of Jersey heifers. Iranian J. Appl. Anim. Sci. 8(3), 397-405.
Hartadi H., Kearl L.C., Reksohadiprojo S., Harris L.E. and Lebdosukoyo S. (1980). Tabel-Tabel dari Komposisi Bahan Makanan Ternak untuk Indonesia. Data Ilmu Makanan untuk Indonesia. International Feedstuffs Institute Utah Agricultural Experiment Station. Utah.
Haryanto B. (2012). Perkembangan penelitian nutrisi ruminansia. Wartazoa. 22(4), 169-177.
Jeong C.D., Mamuad L.L., Kim S.H., Choi Y.J., Soriano A.P., Cho K.K., Jeon C.O., Sung-Sil L. and Sang-Suk L. (2015). Effect of soybean meal and soluble starch on biogenic amine production and microbial diversity using in vitro rumen fermentation. Asian-Australasian J. Anim. Sci. 28(1), 50-57.
Karlsson L., Ruiz-Moreno M., Stern M.D. and Martinsson K. (2012). Effects of temperature during moist heat treatment on ruminal degradability and intestinal digestibility of protein and amino acids in hempseed cake. Asian-Australasian J. Anim. Sci. 25(11), 1559-1567.
Lin C. and Kung L. (2015). Heat-treated soybeans and soybean meal in ruminant nutrition. Ph D. Thesisi. University of Delaware. Newark, Dolaware, United States.
McDonald P., Edwards R.A., Greenhalgh J.F.D., Morgan C.A., Sinclair L.A. and Wilkinson R.G. (2011). Animal Nutrition. Pearson Canada Inc., Toronto, Canada.
Millen D.D., Arrigoni M.D.B. and Pacheco R.D.L. (2016). Rumenology. Springer International Publishing, Cham, Switzerland.
Mjoun K., Kalscheur K.F., Hippen A.R. and Schingoethe D.J. (2010). Ruminal degradability and intestinal digestibility of protein and amino acids in soybean and corn distillers grains products. J. Dairy Sci. 93, 4144-4154.
Mudita I.M., Kayana I.G.N. and Wirawan I.W. (2016). Rumen fermentation of bali cattle fed basal diet with biosupplement of cattle colon and organic waste bacteria consortium. Int. J. Environ. Agric. Res. 2(6), 2455-6939.
Murray R.K., Bender D.A., Botham K.M., Kennelly P.J., Rodwell V.W. and Weil P.A. (2009). Harper’s Illustrated Biochemistry. McGraw Hill, New York.
Nedelkov K.V. (2019). In situ evaluation of ruminal degradability and intestinal digestibility of sunflower meal compared to soybean meal. Iranian J. Appl. Anim. Sci. 9(3), 395-400.
Nobar R.S. (2011). Ruminal dry matter degradability of treated soybean meal as source of escape protein. African J. Biotechnol. 10(41), 8090-8092.
Noviandi C.T., Eun J.S., Peel M.D., Waldron B.L., Min B.R., Zobell D.R. and Millers R.L. (2014). Effect of energy supplementation in pasture forages on in vitro ruminal fermentation characteristics in continuous cultures. Prof. Anim. Sci. 30, 13-22.
Owens N.F. and Basalan M. (2016). Ruminal fermentation. Springer International Publishing, Cham, Switzerland.
Pilachai R., Schonewille J.T., Thamrongyoswittayakul C., Aiumlamai S., Wachirapakorn C., Evert H. and Hendriks W.H. (2011). The effects of high levels of rumen degradable protein on rumen pH and histamine concentration in dairy cows. J. Anim. Physiol. Anim. Nutr. 96(2), 206-213.
Plummer D.T. (1971). An Introduction Practical Biochemistry. McGraw-Hill Book Company Ltd., London, United Kingdom.
Scanes C. (2010). Fundamentals of Animal Science. Delmar Cengage Learning. New York.
Schmidt J. and Zsedely E. (2011). Nutrition of Ruminants. University of West-Hungary, Sopron, Hungary.
Steel R.G.D. and Torrie J.H. (1980). Principles and Procedures of Statistics: A Biometrical Approach. McGraw Hill, New York.
Stern M.D., Bach A. and Calsamiglia S. (2006). New concepts in protein nutrition of ruminants. Pp. 45-66 in Proc. Ann. Southwest Nutr. Manag. Conf., Tempe, Arizona.
Suhartanto B., Utomo R., Kustantinah Budisatria I.G.S., Yusiati L.M. and Widyobroto B.P. (2014). Pengaruh penambahan formaldehid pada pembuatan undegraded protein dan tingkat suplementasinya pada pelet pakan lengkap terhadap aktivitas mikroba rumen secara in vitro. Bullet. Anim. Sci. 38(3), 141-149.
Tilley J.M.A. and Terry R.A. (1963). A two-stage technique for in vitro digestion of forage crop. Grass Forage Sci. 18, 104-111.
Vanegas J.L., Carro M.D., Alvir M.R. and Gonzalez J. (2016). Protection of sunflower seed and sunflower meal protein with malic acid and heat: Effects on in vitro ruminal fermentation and methane production. J. Sci. Food Agric. 97, 350-356.
Wiryawan K.G., Saefudin A., Fuah A.M., Priyanto R., Khotijah L., and Suharti S. (2017). Fermentation characteristics and nitrogen retention of Madura cattle fed complete rations containing soybean pod and by-products. Med. Peternak. 40(1), 28-34.