Association of PIT1 Gene with Milk Fat Percentage in Holstein Cattle

الموضوعات :

ز. ابراهیمی حسین‌زاده ¹ (Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran)
م.ر. محمدآبادی ² (Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran)
ع. اسمعیلی‌زاده ³ (Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran)
الف خضری ⁴ (Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran)
ع. نجمی نوری ⁵ (Department of Animal Science,Yasouj University,Yasouj,Iran)

تاريخ الإرسال : 01 الأربعاء , ذو القعدة, 1435 تاريخ التأكيد : 22 السبت , محرم, 1436 تاريخ الإصدار : 17 الثلاثاء , ذو القعدة, 1436

الکلمات المفتاحية: PCR, polymorphism, milk fat, Holstein cattle, PIT1 gene,

ملخص المقالة :

The pituitary-specific transcription factor (PIT1) gene is responsible for pituitary development and hormone secreting gene expression in mammals. PIT-1 is studied as a candidate genetic marker for growth, carcass and also for milk yield traits. In dairy farm animals, the principal goal of the selection is the improvement of milk yield and composition. The genes of milk proteins and hormones are excellent candidate genes for linkage analysis with quantitative trait loci (QTL) because of their biological significance on the quantitative traits of interest. Thus, in this study association between polymorphism of the pituitary transcription factor 1 (PIT1) gene and milk fat percentage of Holstein cattle in Khorasan Razavi province of Iran were analyzed. A total of 100 dairy cows from a herd containing 1000 animals were included in the study. Genomic DNA was extracted from the whole blood. One pair primers were used for amplification of PIT1 gene and PCR products were electrophoresed on 1% agarose gel. Then, PCR products were digested with HinfI restriction enzyme. Results were analyzed using PopGene software and allele frequencies A and B were 0.25 and 0.75, respectively. Frequencies of AA, AB and BB genotypes, number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected hemozygosity, observed hemozygosity, Nei’s index and Shanon’s index were 6, 40 and 54%, 2, 1.6, 0.37, 0.40, 0.37, 0.62, 0.59, 0.37 and 0.56, respectively. Results of k-square shown that population is in Hardy-Weinberg equilibrium. SAS software with GLM procedure was used for calculation of association between milk fat percentage and observed genotypes and results indicated that the effect of genotype on fat percentage was significant (P<0.01) and AB genotype had the highest effect on milk fat percentage. These results imply that the PIT1 genotypes affected milk fat percentage, suggesting that this polymorphism can be used as a molecular marker for this trait.

المصادر:

Arora R. and Bhatia S. (2004). Genetic structure of Muzzafarnagri sheep based on microsatellite analysis. Small Rumin. Res. 54, 227-230.

Aytekin İ. and Boztepe S. (2013). Associations of PIT1gene polymorphism with milk yield and composition traits in brown swiss cattle. J. Anim. Plant Sci. 23, 1281-1289.

Beigi Nassiri M.T., Biranvand Z., Hartatik T., Fayazil J. and Tavakoli S. (2010). The study of PIT1gene polymorphism in the Najdi cattle using PCRRFLP method. J. Anim. Vet. Adv. 9, 2001-2003.

Bona G., Paracchini R., Giordano M. and Momigliano-Richiardi P. (2004). Genetic defects in GH synthesis and secretion. EuropeanJ. Endocrinol. 151, 3-9.

Camper S.A., Saunders T.L., Katz R.W. and Reeves R.H. (1990). The PIT1transcription factor gene is a candidate for the murine Snell dwarf mutation. Genomics. 8, 586-590.

Carrijo S.M., De Alencar M.M., Toral F.L.B. and De Almedia Regitano L.C. (2008). Association of PIT1genotypes with growth traits in Canchim cattle. Sci. Agric. 65, 116-121.

Curi, R.A., Palmieri D.A., Suguisawa L., de Oliviera H.N., Silveira A.C. and Lopes C.R. (2006). Growth and carcass traits associated with GH1/AluI and POU1F1/HinfI gene polymorphisms in Zebu and crossbred beef cattle. Genet. Mol. Biol. 29, 56-61.

De Mattos K.K., Del Lama S.N., Martinez M.L. and Freitas A.F. (2004). Association of bGH and PIT1gene variants with milk production traits in dairy Gyr bulls. Pesqui. Agropecu. Bras. 39, 147-150.

Dierkes B., Kriegesmann B., Baumgartner B.G. and Brening B. (1998). Partial genomic structure of the bovine PIT1gene and characterization of a HinfI transition polymorphism in exon 6. Anim. Genet. 29, 405.

Dybus A., Kmieć M., Sobek Z., Pietrzyk W. and Wiśniewski B. (2003). Associations between polymorphism of growth hormone releasing hormone (GHRH) and pituitary transcription factor 1 (PIT1) genes and production traits of Limousine cattle. Arch. Tierz. 46, 527-534.

Dybus A., Szatkowska I., Czerniawska-Piątkowska E., Grzesiak W., Wojcik J., Rzewucka E. and Zych S. (2004). PIT-1-HinfI gene polymorphism and its associations with milk production traits in polish Black-and-White cattle. Arch. Tierz. 47, 557-563.

Edriss V., Edriss M.A., Rahmani H.R. and Sayed-Tabatabaei B.E. (2008). PIT1gene polymorphism of Holstein cows in Isfahan Province.Biotechnology. 7, 209-212.

FAO. (1993). Survey of present status of the use of artificial insemination in developing countries. World Anim. Rev. 75, 26-35.

Haugen B.R., Wood W.M., Gordon D.F. and Ridgeway E.C. (1993). A thyrotrope-specific variant of PIT1transactivates the thyrotropin β promoter. J. Biol. Chem. 268, 20818-20824.

Heravi-Moussavi A.R. and Danesh-MesgaranM. (2009). Effect of diverse sire origins on first-parity performance in Iranian Holstein cows. Res. J. Biol. Sci. 4(7), 800-806.

Hori-Oshima S. and Barreras-Serrano A. (2003). Relationships between DGAT1 and PIT1Genes Polymorphism and Milk Yield in Holstein Cattle, Proceedings, Western Section. American Society of Animal Science.

Javanmard A., Asadzadeh N., Banabazi M.H. and Tavakolian J. (2005). The allele and genotype frequencies of bovine pituitary specific transcription factor and leptin genes in Iranian cattle and buffalo populations using PCR-RFLP. Iranian J. Biotechnol. 3, 2.

Jawasreh K.I.Z., Awawdeh F., Rawashdeh I., Hejazeen F. and Al-Talib M. (2009). The allele and genotype frequencies of bovine pituitary specific transcription factor and leptin genes in Jordanian cattle population by using PCR-RFLP. Australian J. Basic Appl. Sci. 3, 1601-1606.

Kopp P. and Jameson J.L. (1998). Thyroid disorders. Pp. 459-473 in: Principles Of Molecular Medicine. J.L. Jameson and F.S. Collins, Eds. Humana Press. Totowa NJ.

Li S., Crenshaw R.D., Rawson E.J., Simmons D.M., Swanson L.W. and Rosenfeld M.G. (1990). Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene PIT1. Nature. 357, 528-533.

Moody D.E., Pomp D. and Berendse W. (1995). Restriction fragment length polymorphism in amplification products of bovine PIT1 gene and assignment of PIT1 to bovine chromosome 1. Anim. Genet. 26, 45-47.

Mukesh M., Sodhi M., Sobti R.C., Prakash B., Kaushik R., Aggarwal R.A.K. and Mishra B.P. (2008). Analysis of bovine pituitary specific transcription factor-HinfI gene polymorphism in Indian zebuine cattle. Livest. Sci. 113, 81-86.

Nei M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natio. Acad. Sci. 70, 3321-3323.

Nei M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89, 583-590.

Oprzadek J., Flisikowski K., Zwierzchowski L. and Dymnicki E. (2003). Polymorphisms at loci of Leptin (LEP), PIT1 and STAT5A and their association with growth, feed conversion and carcass quality in black-and-white bulls. Anim. Sci. Pap. Rep. 21, 135-145.

Othman E., Fawzia A., Zayed A., El Gawead A. and Medhat El-Rahman R.A. (2011). Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo. J. Genet. Engin. Biotechnol. 9, 97-102.

Özdemir M. (2012). Determination of Pit-1/Hinf1 polymorphism in Holstein and Native Ear cattle raised as genetic resource in Turkey. J. Anim. Plant Sci. 22, 25-28.

Parmentier I., Portetelle D., Gengler N., Pradi A., Bertozzi C., Vleurick L., Gilson R. and Renaville R. (1999). Candidate gene markers associated with somatotropic axis and milk selection. Domes. Anim. Endocrinol. 17, 139-148.

Renaville R., Gengler N., Vrech E., Prandi A., Massart S., Corradini C., Bertozzi C., Mortiaux F., Burny A. and Portetelle D. (1997a). PIT1gene polymorphism, milk yield, and conformation traits for Italian Holstein-Friesian bulls. J. Dairy Sci. 80, 3431-3438.

Renaville R., Gengler N., Parmentier I., Mortiaux F., Massart S., Bertozzi C., Burny A. and Portetelle D. (1997b). PIT1gene HinfI RFLP and growth traits in doublemuscled Belgian Blue cattle. J. Anim. Sci. 75(1), 146.

SAS Institute. (2002). SAS/STAT 9 User's Guide –Procedures, SAS Inst. Inc., Cary, NC.

Shook G.E. (2006). Major advances in determining appropriate selection goals. J. Dairy Sci. 89, 1349-1361.

Soyeurt H., Dardenne P., Gillon A., Croquet C., Vanderic S., Mayeres P., Bertozzi C. and Gengler N. (2006). Variation in fatty acid contents of milk and milk fat within and across breeds. J. Dairy Sci. 89, 4858-4865.

Vargas L.D., Gana V.E. and Escudero I.F. (2004). PIT1gene polymorphism in dairy cows from Central Chile. Arch. Zoot. 53, 217-220.

Viorica C., Vlaic A. and Gaboreanu I. (2007). HinfI polymorphism of K-Casein and PIT1genes in Romanian Simmental Cattle. Lucrări Stiinţifice Zootehnie Si Biotehnologiy. 40, 59-61.

Xue K., Chen H., Wang S., Cai X., Liu B., Zhang C.F., Lei C.Z., Wang X.Z., Wang Y.M. and Niu H. (2006). Effect of genetic variations of the POU1F1 gene on growth traits of nanyang cattle.Acta Genet. Sinica. 33, 901-907.

Yan L.J., Liu B., Fang X.T., Chen H., Zhang R.F., Bao B. and Zhang H.J. (2006). Analysis of POU1F1 gene polymorphisms in Qinchuan cattle and Chinese Holstein cattle. Yi Chuan. 28(11), 1371-1375.

Yeh F.C., Yang R. and Boyle T. (1999). Pop Gene Version 1.31. Microsoft Window–based free software for population genetic analysis, University of Alberta. Edmonton, AB, Canada.

Zakizadeh S., Reissmann M., Rahimi G., Javaremi N.A., Reinecke P., Mirae-Ashtiani S.R. and Shahrbabak M.M. (2007). Polymorphism of bovine POU1F1 gene: allele frequencies and effects on milk production in three Iranian native breeds and Holstein cattle of Iran. Pakistan J. Biol. Sci. 10, 2575-2578.

Zhang C., Liu B., Chen H., Lan X., Lei C., Zhang Z. and Zhang R. (2009). Associations of a HinfI PCRRFLP of POU1F1 gene with growth traits in Qinchuan cattle. Anim. Biotechnol. 20, 71-74.

Zhao Q., Davis M.E. and Hines H.C. (2004). Associations of polymorphisms in the PIT1gene with growth and carcass traits in Angus beef cattle. J. Anim. Sci. 82, 2229-2233.