A Study on the Effects of Chilling Period on Sperm Quality of Sannan and Jamunapari Goats
الموضوعات :تی. اسواراموهان 1 , کا. نیلانی 2 , پی. سورکا 3 , پی. ماهادوان 4 , ک. بالاسوبرامانیام 5
1 - Department of Zoology, Faculty of Science, University of Jaffna, Jaffna, Sri Lanka
2 - Department of Zoology, Faculty of Science, University of Jaffna, Jaffna, Sri Lanka
3 - Department of Zoology, Faculty of Science, University of Jaffna, Jaffna, Sri Lanka
4 - Artificial Insemination Centre, Thirunelvely, Jaffna, Sri Lanka
5 - Bio Tec, Thirunelvely, Jaffna, Sri Lanka
الکلمات المفتاحية: viability, chilling period, goat semen, Velocity,
ملخص المقالة :
The objective of the present study was to examine the effects of chilling period on semen quality of Sannan and Jamunapari. Semen of goat breeds were collected with artificial vagina and chilled at 5-9˚C. Volume, colour and pH were determined on fresh semen. After dilution, microscopic examination was performed for the progressive motility and sperm viability. Diluted semen of both breed was compared by microscopic examination from day 0 to 3 during chilling. Hemocytometer was loaded with 10 µL of semen to evaluate the sperm count. Eosin stain (1%) was used to assess the viable sperm. Although viability and sperm velocity of both goat breed decreased(P<0.05) in a time dependent manner during chilling, the viability between the Sannan and Jamunapari did not differ significantly. However there was a significant difference for means of sperm velocity (P<0.05). Progressive motility and viability decreased gradually during chilling at 5-9˚C.In order to obtain higher successful rate of insemination, chilled goat semen should be supplied to the farmers within a day.
Abeygunawardena H., Alexander P.A.B.D. and Abeygunawardena I.S. (2001). Artificial insemination of cattle in Sri Lanka: status, performance and problems. Animal Production and Health Section International Atomic Energy Agency, Vienna, Austria.
Aitken R.J. (1990). Motility Parameters and Fertility. Pp. 285-302 in Controls Sperm Motility: Biological and Clinical Aspects. C. Gagnon, Ed. Boca Raton: CRC Press.
Budsworth P.R., Amann R.P. and Chapman P.L. (1988). Relationship between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility. J. Androl. 9, 41-53.
Hidalgo M., Rodriguez I. and Dorado J.M. (2007). The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability. Anim. Reprod. Sci. 100, 61-72.
Holt W.V. and North R.D. (1984). Partially irreversible cold-induced lipid phase transitions in mammalian sperm plasma membrane domains: freeze-fracture study. J. Exp. Zootech. 230, 473-483.
Iguer Ouada M. (2001). Medically assisted procreation in Canine species: analyses and 4 ˚C preservation of semen. Ph D Thesis. Univ. de Liège, Liège.
Kirk E.S., Squires E.L. and Graham J.K. (2005). Comparison of in vitro laboratory analyses with the fertility of cryopreserved stallion spermatozoa. Theriogenology. 64, 1422-1439.
Leboeuf B., Restall B. and Salamon S. (2000). Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci. 62, 113-141.
Lopez A., Söderquist L. and Rodriquez-Martinez H. (1999). Sperm viability in ram semen diluted and stored in three different extenders. Acta. Vet. Scand. 40, 1-9.
Salamon S. and Robinson T.J. (1962). Studies on the artificial insemination of Merino sheep. II. The effects of semen diluents and storage on lambing performance. Australian J. Agric. Res. 13, 271-281.
Sarma P.V., Reddy I.J. and Sarma P.A. (1996). Bovine spermatozoan motility behaviour in cervical mucus and its relationship to fertility. Indian J. Anim. Reprod. 17, 137-137.
Schindler H. and Amir D. (1961). Longevity of ram sperm in various diluents and different dilution rates. J. Agric. Sci. 56, 183-189.
Tardiff A.L., Farrell P.B., Trouern Trend V. and Foote R.H. (1997). Computer assisted sperm analysis for assessing initial semen quality and changes during storage at 5 ˚C. J. Dairy Sci. 80, 1606-1612.
Udeh I. and Oghenesode B. (2011). Effects of type of extender and storage conditions on the motility of goat spermatozoa. Int. J. Anim. Vet. Adv. 3(5), 282-286.