A comprehensive review on morphological, genetic and phytochemical diversity, breeding and bioprospecting studies of genus Chlorophytum Ker Gawl. from India

Subject Areas : Pharmacognosy

Ajay Sarjerao  Nalawade ¹ (Sanjay Ghodawat University, Kolhapur-416118 MS, India)
Rajaram Vithoba  Gurav ² (Director of Student Development and Department of Botany, Shivaji University, Kolhapur-416004 MS, India)
Abhinandan Ravsaheb  Patil ³ (Sanjay Ghodawat University, Kolhapur-416118 MS, India)
Mohsina  Patwekar ⁴ (Luqman college of Pharmacy, PB 86, P and T colony, Old Jewargi Road, Gulbarga-585102, India)
Faheem  Patwekar ⁵ (Luqman college of Pharmacy, PB 86, P and T colony, Old Jewargi Road, Gulbarga-585102, India)

Received: 2022-01-10 Accepted : 2022-03-25 Published : 2022-05-01

Keywords: Morphology, genetic, India, Phytochemical, Bioprospecting, Chlorophytum Ker Gawl,

Abstract :

India presently comprises 23 species of Chlorophytum which are commercially utilized as ‘White gold’ or ‘Divya aushadhi’. There are pressing demands on the biodiversity and bioresources of Western Ghats and Chlorophytum is one of the highly demanded medicinal entities, as Chlorophytum has export value and there is a vast demand for its roots under the trade name ‘Safed Musali’. The roots of various species are collected from nature. Even all the species of Chlorophytum are not studied properly for taxonomy, morphology, medicinal value and antioxidant contents. The present review proposes a comprehensive impression of phytogeographical survey, morphological, genetic and phytochemical diversity, medicinal properties and uses, field studies and breeding practices of genus Chlorophytum. Conclusively, the anticipated article is an endeavour to provide a complete update of several studies led by members of the genus Chlorophytum that will possibly be utilized systematically and appropriately in different conservation strategies and sustainable development.

References:

Aberoumand, A., 2010. Comparison of chemical analysis of Asparagus officinalis and Chlorophytum comosum; Iranian plant foods. Indian J. Agric. Res. 44(1), 58-61.
Acharya, D., Mitaine-Offer, A.C., Kaushik, N., Miyamoto, T., Paululat, T., Mirjolet J. F., Duchamp, O., Lacaille-Dubois, M.A., 2009. Cytotoxic spirostane-type saponins from the roots of Chlorophytum borivilianum. J. Nat. Prod. 72 (1), 177-181.
Acharya, D., Mitaine-Offer. A.C., Kaushik, N., Miyamoto, T., Paululat, T., Lacaille-Dubois, M.A., 2008. Furostane-type steroidal saponins from the roots of Chlorophytum borivilianum. Helv. Chim. Acta. 91(12), 2262-2269.
Acharya, S., 2013. Control of the potato virus X through application of root extracts of Chlorophytum nepalense to potato plants and tubers. Potato Res. 56, 1-10.
Adsul, A.A., 2015. Taxonomic revision of genus Chlorophytum Ker Gawl. for India, Ph. D. Thesis Submitted to Shivaji University, Kolhapur.
Adsul, A.A., Lekhak, M.M., Yadav S.R., 2014. Chlorophytum sharmae (Asparagaceae): a new taxon from Kerala, India. Kew Bull. 69, 9503
Adsul, A.A., Patil, S.A., Yadav, S.R. Chlorophytum kolhapurense Distribution, Phenology and Morphological variations. K. S. Manilal Award Paper in XXIII Annual Conference of Indian Angiosperm Taxonomy and National Seminar on Recent Advances in Plant Taxonomy Research, 27th to 29th December 2013.
Agricare: Safed Musali cultivation, www.agricare.org 2005 - 2006. Site Developed and maintained by: CyberXel.
Alim, I., Arora, H., Rafi, M., 2010. Purity, strength and trace elements contents evaluation of Chlorophytum borivilianum for export purpose. Orient J. Chem. 26, 259-262
APG III, 2009. An update of the Angiosperm phylogeny group classification for the orders and families of flowering plants: APG III. Bot. J. Linn. Soc. 161(2), 105-121.
Asolkar, L.V., Kakkar, K.K., Chakra, O.J., 1992. Second Supplement to Glossary of Indian Medicinal Plants with Active Principles Part-1 (A-K) 1965-1981. NISC, CSIR, New Delhi, India, pp. 265-266.
Aundhe, D.J., Deokule, S.S., 2001. Studies on root morphology of Chlorophytum. Ker-Gawl. J. Phytol. Res. 14(2), 195-197.
Bahmania, M., Zargaranb, A., Rafieian-Kopaeic, A., 2014. Identification of medicinal plants of Urmia for treatment of gastrointestinal disorders. Rev. Bras. Farmacogn. 24(4), 468-480.
Bahulikar, R.A., Lagu, M.D., Kulkarni, B.G., Pandit, S.S., Suresh, H.S., Rao, M.K.V., Ranjekar, P.K., Gupta, V.S., 2004. Genetic diversity among spatially isolated populations of Eurya nitida Korth. (Theaceae) based on inter-simple sequence repeats. Curr. Sci. 86, 824-831.
Baker, J.G., 1876. Revision of the genera and species of Anthericeae and Eriospermae. Bot. J. Lin. Soc. 15, 253-363.
Barrett, S.R.H., Kohn, J., 1991. Genetic and evolutionary consequences of small population size in plants: implications for conservation, In: Falk DA, Holsinger KE, Eds. Genetics and conservation of rare plants. New York: Oxford University Press. pp. 3-30.
Basu, S., Jha, T.B., 2008. Chlorophytum nepalense (Lindl.) Baker — an unexplored plant of potential economic value? Curr. Sci. 95(4), 439.
Bathoju, G., Giri, A., 2012. Production of medicinally important secondary metabolites (Stigmasterol and Hecogenin) from root cultures of Chlorophytum borivilianum (Safed musli). Recent res. Sci. Technol. 4(5), 45-48.
Batugal, P.A., Kanniah, J., Lee, S.Y., Oliver, J.T., (eds), 2004. Medicinal Plants Research in Asia, Volume 1: The Framework and Project Workplans. International Plant Genetic Resources Institute - Regional Office for Asia, the Pacific and Oceania (IPGRI-APO), Serdang, Selangor DE, Malaysia.
Bianco, A., Venditti, A., Foddai, S., Toniolo, C., & Nicoletti, M., 2014. A new problem. Contamination of botanicals by phthalates. Rapid detection tests. Nat. Prod. Res. 28(2), 134-137.
Bjora, C.S., Elden, M., Nordal, I., Brysting, A.K., Awas, T., Demissew, S., Bendiksby, M., 2017. Speciation in the genera Anthericum and Chlorophytum (Asparagaceae) in Ethiopia - A molecular phylogenetic approach. Phytotaxa 297(2), 139-156.
Bjora, C.S., Hoell, G., Kativu, S., Nordal, I., 2008. New taxa of Chlorophytum (Anthericaceae) from southern tropical Africa with notes on their sister group relationships. Bot. J. Linn. Soc. 157, 223-238.
Bodare, S., Tsuda, Y., Ravikanth, G., Uma Shaanker, R., Lascoux, M., 2013. Genetic structure and demographic history of the endangered tree species Dysoxylum malabaricum (Meliaceae) in Western Ghats, India: implications for conservation in a biodiversity hotspot. Ecol. Evol. 3(10), 3233-3248.
Bodele, M.A., 2016. Comparative Studies on Root Tubers of Indian Species of Chlorophytum and Aphrodisiac Ayurvedic Drug ‘Safed Musli’ of Indian Market, Ph. D. Thesis submitted to University of Mumbai.
Bonnefont-Rousselot, D., 2004. The role of antioxidant micronutrients in the prevention of diabetic complications. Ther. Adv. Endocrinol. 3(1), 41.
Borah, D., Kafley, P., Das, A.P., Tangjang, S., Averynov, L., 2019. Chlorophytum assamicum (Asparagaceae), a new species from Northeast India. Phytotaxa 394 (1), 123-125.
Bordia, P.C., Joshi, A., Simlot, M.M. in K.L. Chadha and Gupta, R. (Eds.), 1995. Safed Musali Advances in Horticulture, Medicinal and Aromatic Plants. Malhotra Publishing House New Delhi, Vol. 11.
Canter, P.H., Thomas, H., Ernst, E., 2005. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol. 23(4), 180-185.
Chakraborthy, G.S., Aeri, V., 2009. Phytochemical and antimicrobial studies of Chlorophytum borivilianum. Int. J. Pharm. Sci. Drug Res. 1(2), 110-112.
Chakraborthy, G.S., Aeri, V., 2010. Pharmacognostical studies of potential herb- Chlorophytum borivilianum. Int. J. Pharm. Sci. Res. 1(4), 89-95.
Chandore, A.N., Malpure, N.V., Adsul, A.A., Yadav, S.R., 2012. Chlorophytum belgaumense, a new species of Asparagaceae from the Western Ghats of India. Kew Bull. 67, 527-531.
Chandore, A.N., Yadav, S.R., 2019. Chlorophytum tillariense (Asparagaceae), a new species from Western Ghats of India. Phytotaxa 427(4), 279-284.
Chauhan, R., Quraishi, A., Jadhav, S.K., Keshavkant, S., 2016. A comprehensive review on pharmacological properties and biotechnological aspects of genus Chlorophytum. Acta Physiol. Plant. 38, 1-14.
Chopra, R.N., Nayar, S.L. Chopra, I.C., 1956. Glossary of Indian Medicinal Plants. C.S.I.R.. New Delhi. pp. 67.
Chua, B.L., Abdullah, Z., Pin, K.Y., Abdullah, L.C., Choong, T.S.Y., Yusof, U.K., 2017. Isolation, structure elucidation, identification and quantitative analysis of 1’-Acetoxychavicol (Aca) from the roots of Chlorophytum boriviliuanum (Safed musli). J. Eng. Sci. Technol. 12(1), 198-213.
Dabur. R., Gupta, A., Mandal, T.K., Singh, D.D., Bajpai, V., Gurav, A.M., Lavekar, G.S., 2007. Antimicrobial activity of some Indian medicinal plants. Afr. J. Tradit. Complement. Altern. Med. 4(3), 313-318.
Deore, S.L., Jajoo, N.B., Chittam, K.P., Deshmukh, T.A., 2015. Comparative pharmacognostic, phytochemical and biological evaluation between five Chlorophytum species. Pharmacogn. J. 7(5), 317-325.
Deore, S.L., Khadabadi, S.S., 2009. Standardisation and Pharmaceutical Evauation of Chlorophytum borivalianum Mucilage. Rasayan J. Chem. 1(4), 887-892.
Deore, S.L., Khadabadi, S.S., 2010. In vitro anthelmintic studies of Chlorophytum borivilianum Sant. & Fernandez tubers. Indian J. Nat. Prod. Resour. 1(1), 53-56.
Deore, S.L., Khadabadi, S.S., 2010. Isolation and characterization of phytoconstituents from Chlorophytum borivilianum. Pharmacogn. Res. 2(6), 343-349.
Dwivedi, R., 2013. The study of morphological characteristics of Chlorophytum borivilianum accessions from Chhattisgarh natural habitats. Int. J. Agric. Sci. Res. 3 (4), 31-38.
Dwivedi, R., Sharma, D.K., 2011. Assessment of genetic diversity among collected genotypes of Chlorophytum borivilianum using random amplified polymorphic DNA (RAPD) markers. Afr. J. Biotechnol. 10(27), 5268-5272.
Gao, Y.H., MEI, Q.X., Wu, H.F., Diao, Y.M., Peng, X.S., Zhang, R.F., 2005. Chlorophytoside A, a new labdane diterpene glycoside from Chlorophytum laxum. Chin. Chem. Lett. 16(7), 925-927.
Garg, N., 2010. Morphological, Biochemical Studies and RAPD Analysis for Varietal Identification in Safed musli (Chlorophytum borivilianum) Cultivars. Thesis, Ch. Charan Singh University, Meerut.
Ghosal, Shibnath, 2006. Composition for Anti-Obesity, Health Restorative and Health Promotional Benefits. Patent No. WO/2006/036638 A1.
Govaerts, R., Zonneveld, B.J.M., Zona, S.A., 2012. World Checklist of Asparagaceae. Royal Botanic Gardens, Kew.
Gunn, C.R., Seldin, M.J., 1976. Seeds and fruits of North American Papaveraceae. Tech. Bull. 1517.
Harish Gupta, A.K., Phulwaria, M., Rai, M.K., Shekhawat, N.S., 2014. Conservation genetics of endangered medicinal plant, Commiphora wightii in Indian Thar Desert. Gene 535(2), 266-272.
Hooker, J.D., 1892. Flora of British India. L. Reeve and Co., London. 6. 332-336.
Howell, J.T., Raven, P.H., Rubtzoff, P., 1958. Flora of San Francisco. Wasman. J. Biol. 16, 1-155.
Jacqueline and John., “Chlorophytum amaniense ‘Fire Flash’, the glowing beauty” Posted by Jacqueline on November 30th, 2008. in Plants database https://www.jaycjayc.com/chlorophytum-amaniense-fireflash/#.XLi8iOgzbIU
Jay, B., Bhoot, S., Nariya, P, Savalia, V., Pandya, D., 2021. Isolation and characterization of marker compound for detecting adulteration of Chlorophytum Species in Asparagus racemosus, Research J. Pharm. Tech. 14(2), 800-802.
Jaykumar, B., Sagar, S.K., Sinnathurai, G., 2008. Neutraceutical compositions and methods of use of Safed Musli extracts. Patent No. WO/2008/020451 A2.
Joshi, A., Rajamani, G., Kaswan, V., 2013. Seperation of saponins from Chlorophytum borivilianum by high performance liquid chromatography. Adv. Appl. Res. 5(1), 78-80.
Kagale, L.A., 2016. Survey and Nutritional Status of Wild Leafy Vegetables Along the Caostal Region of Ratnagiri and Sindhudurg. Ph.D. Thesis, Submitted to Shivaji University, Kolhapur.
Kale, K., Thakare, P., 2013. Genus Chlorophytum Ker Gawl.: Medicinally important plant from Ancient Indian literature (Ayurveda). Int. J. Ayurvedic herb. Med., 3(3), 1201-1211.
Kale, K.A., Deogade, M.S., 2016. DNA Bar code: - New approach for authentication of medicinal plant. Joinsismed 4(4), 185-187.
Kale. K., 2016. DNA Bar Coding of selected Chlorophytum Spp. Ph.D. Thesis submitted to Sant Gadge Baba Amravati University, Amravati.
Karp, A., Edwards, K., 1995. Molecular techniques in the analysis of the extent and distribution of genetic diversity. In: Ayad, W.G., Hodgkin, T., Jaradat, A., Rao, V.R., (Ed.), Molecular Genetic Techniques for Plant Genetic Resources. Report of an IPGRI Workshop 9-11 October 1995, Rome. International Plant Genetic Resources Institute. Rome, pp. 11-38.
Kativu, S., Nordal, I., 1993. New combinations of African species in the genus Chlorophytum (Anthericaceae). Nord. J. Bot. 13(1), 59 -65.
Katoch, M., Kumar, R., Pal, S., Ahuja, A., 2010. Identification of Chlorophytum species (C. borivilianum, C. laxum, C. capense and C. comosum) using molecular markers. Ind. Crops Prod. 32(3), 389-393.
Kaur, R., Arora, S., Thukral, A.K., 2015. Quantitative and qualitative analysis of saponins in different plant parts of Chlorophytum borivilianum. Int. J. Pharma Bio Sci. 6(1), 826-835.
Kayang, H., 2007. Triblal Knowledge on medicinal plants of Meghalaya, Northeast India. Indian J. Tradit. Knowl. 6(1), 177-181.
Kirtikar, K.R., Basu, B.D., 1994, Indian Medicinal Plants. 2nd Ed. Vol IV. Deharadun, M/s Bishensingh Mahendra Palsingh. pp. 2509.
Kite, G.C., Grayer, R.J., Rudall, P.J., Simmonds, M.S.J., 2000. The potential for chemical characters in Monocotyledon systemaics, Monocots: Systematics and Evolution. Eds K. L. Wilson and D. A. Morrison (CSIRO: Melbourne), pp. 101-113.
Kothari, S.K., Singh, K., 2004. Safed moosli (Chlorophytum borivilianum) revisited. J. Med. Aromat. Plant Sci. 26(1), 60-63.
Kubitzki, K., Huber, H., Rudall, P.J., Stevens, P.S., Stutzel, T., 1998. The Families and Genera of Vascular Plants Volume III Flowering Plants Monocotyledons Lilianae (except Orchidaceae). Springer- Verlag Berlin Hiedenberg, New York, pp. 114-125.
Kumar, B., Verma, A.K., Singh, H.P., Misra, H.O., Lal, R.K., 2010. Genetic divergence and correlations study in Chlorophytum borivilianum. J. N. Seeds. 19(4), 321-329.
Kumar, M., Priyadarshi, M., Verma, S., Kumar, M., Kumar, A., 2010. Anti-tumour, anti-mutagenic and chemomodulatory potential of Chlorophytum borivilianum. Asian Pac. J. Cancer Prev. 11(2), 327-334.
Kumari, B., Mallick, M.A., Vyas, G.K., 2014. Assessment of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in different agro-climatic regions of India revealed by random amplified polymorphic primer (DNA Marker). Int. J. Biodivers. Conserv. 6(12), 854-860.
Lakshmi, V., Kumar, R., Pandey, K., Joshi, S.B., Roy, R., Kunnath, P.M., Tiwari, P., Srivastava, A.K., 2009. Structure and activities of a steroidal saponin from Chlorophytum nimonii (Grah) Dalz. Nat. Prod. Res. 23(10), 963-972.
Lekhak, M.M., Adsul, A.A., Yadav, S.R., 2012. Cytotaxonomical investigations into the genus Chlorophytum from India. Kew Bull. 67(2), 285-292.
Mabberley, D.J., 2017. The Plant Book, 4th ed. Cambridge University Press, Cambridge. pp.196.
Maiti, S. (Ed.), 2005. Red listed plant Chlorophytum arundinaceum in National Research Centre For Medicinal & Aromatic Plants Newsletter. The Director, NRC for Medicinal & Aromatic Plants, Boriavi, Anand 387 310, Gujarat. 6(2), 1-6.
Maiti, S., Geetha, K.A., 2005. Characterization, genetic improvement and cultivation of Chlorophytum borivilianum ‐ an important medicinal plant of India, Plant Genet. Resour. 3(2), 264‐272.
Malpure, N.V., Yadav, S.R., 2009. Chlorophytum gothanense, a new species of Anthericaceae from the Western Ghats of India. Kew Bull. 64(4), 739-741.
Manandhar, N.D., 1985. Ethnobotanical notes on certain medicinal plants used by Tharus of Dang-Deokhuri District, Nepal. Int. J. Crude Drug Res. 23(4), 153-159.
Mandal, G.D., Nandi, A.K., 2013. Comparative pharmacognostic and Phytochemical studies of two aphrodisiac plants - Chlorophytum borivilianum Santapau & Fernandes and Chlorophytum tuberosum (Roxb.) Baker. Int. J. Pharm. Pharm. Sci. 5(4), 517-523.
Marais, W., Reilly, J., 1978. Chlorophytum and its related genera (Liliaceae). Kew Bull. 32(3), 653-663.
Mathew, J., George, K.V., 2015. A new Chlorophytum (Asparagaceae) from the southern Western Ghats, Telopea J. Plant Syst. 18, 379-382.
Mathew, K.M., 1983. The Flora of The Tamilnadu Carnatic, The Rapinat Herbarium St. Joseph’s Tiruchirapalli, India, pp. 1641.
Matsushita, H., Kuwabara, H., Ishikawa, S., Mochizuki, M., 2005. Apoptosis Induced in human cell lines by a butanol extract from Chlorophytum comosum roots. J. Helath Sci. 51(3), 341-345.
Meerts, P., Bjora, C.S., 2012. Synopsis of the genus Chlorophytum (Asparagaceae) in Central Africa (Democratic Republic of the Congo, Rwanda, Burundi). Plant Ecol. Evol. 145(3), 373-409.
Mimaki, Y., Kanmoto, T., Sashida, Y., Nishino, A., Satomi, Y., Nishino, H., 1996. Steriodal saponins from the underground parts of Chlorophytum comosum and their inhibitory activity on tumor promoter-induced phospholipids metabolism of HeLa cells. Phytochemistry 41(5), 1405-1410.
Mishra, M., Kotwal, P.C., 2010. Unripe collection of musli (Chlorophytum spp.) and its impact on raw material quality: a case of Dhamtari forest, Chattisgarh, India. Life Sci. Leaf. 3, 79-89.
Mishra, P., Amit Kumar, Nagireddy A., Mani, D.N., Shukla, A.K., Tiwari, R., Sundaresan, V., 2016. DNA barcoding: an efficient tool to overcome authentication challenges in the herbal market. Plant Biotechnol. J. 14, 8-21.
Misra, A., Shasany, A.K., Shukla, A.K., Sundaresan, V., Jain, S.P., Bagchi, G.D., Singh, J., Khanuja, S.P.S., 2006. AFLP-based Detection of adulterants in crude drug preparations of the ‘Safed Musli’ complex. Nat. Prod. Communic. 2(1), 93-97.
Mohammadhosseini, M., Frezza, C., Venditti, A., Mahdavi, B., 2021a. An overview of the genus Aloysia Palau (Verbenaceae): Essential oil composition, ethnobotany and biological activities. Nat. Prod. Res., doi: org/10.1080/14786419.2021.1907576.
Mohammadhosseini, M., Venditti, A., Frezza, C., Serafini, M., Bianco, A., Mahdavi, B., 2021b. The genus Haplophyllum Juss.: Phytochemistry and bioactivities—A review. Molecules 26, 4664.
Mouthe Kemayou, G.P., Fotsing Kache, S., Dzouemo, L.C., M. Happi, G., Fogue Kouam, S., Tchouankeu, J.C., 2021. Phytochemistry, traditional uses, and pharmacology of the genus Ekebergia (Meliaceae): A review. Trends Phytochem. Res. 5(3), 110-125.
Mukherjee, N., 1975. Phytogeography and phylogeny of Chlorophytum Ker-Gawl. (Liliaceae), Bull. Botanic. Soc. Bengal. 29(1), 75-82.
Murthy, K.R.K.,Yoganarasimhan, S.N., 1990. Flora of Coorg, (Kodagu) Karnataka, India. Vismat Publishers, Bangalore, pp. 472.
Nahar, L., El-Seedi, H.R., Khalifa, S.A.M., Mohammadhosseini, M., Sarker, S.D., 2021. Ruta essential oils: Composition and bioactivities. Molecules 26, 4766.
Naik, V.N., 1974. Cytological studies in Chlorophytum bharuchae Ansari, Raghavan et Hemadri. Curr. Sci. 43,161.
Naik, V.N., 1977. Erroneous report of the new basic chromosome number in Chlorophytum. Sci. Cult. 43, 260-261.
Naik, V.N., 1979. Chromosomal behavior in Chlorophytum bharuchae Ansari, Raghavan et Hem. Indian J. Bot. 2, 59-62.
Naik, V.N., 1980. Chlorophytum (Liliaceae) in Indian Garden. J. Indian Bot. Soc. 59, 67-71.
Naik, V.N., Nirgude, S.M., 1980. Reproductive isolation between Chlorophytum glaucum and C. glaucoides. Proc. Plant Sci. 89(6), 465-472.
Naik, V.N., Nirgude, S.M., 1981. Anatomy in relation to taxonomy of Chlorophytum (Liliaceae). Indian J. Bot. 4(2), 48-60.
Nalawade, A.S., 2018. Genetic Diversity and Phytochemical Analysis of Chlorophytum Ker-Gawl. Species. , Ph.D. Thesis Submitted to Shivaji University, Kolhapur.
Nalawade, A.S., Gurav, R.V., 2017. Proximate analysis and elemental composition of seventeen species of Chlorophytum from the Western Ghats. Int. J. Pharm. Sci. Res. 8(7), 131-136.
Nalawade, A.S., Gurav, R.V., 2017. Stomatal studies in the genus Chlorophytum (Asparagaceae). Biosci. Discov. 8(3), 574-581.
Nalawade, A.S., Gurav, R.V., 2020. Genetic Diversity and Phytochemical Analysis of Chlorophytum Species. LAP Lambert Academic Publishing.
Narasimhan, S., Govindarajan, R., Vijayakumar, M., Thakur, M., Dixit, V.K., Mehrotra, S., Madhusudanan, K.P., 2006. Action of (21) fructo-oligopolysaccharide fraction of Chlorophytum borivilianum against streptozotocin-induced oxidative stress. Planta Med. 72(15), 1421-1424.
Nath, V., Kumar, K.P., 2010. Traditional knowledge on ethno-medicinal uses prevailing in tribal pockets of Chhindwara and Betul Districts, Madhya Pradesh, India. Afr. J. Pharm. Pharmacol. 4(9), 662-670.
Nayar, M.P., Sibi, M., Rasiya Beegam, A., 2014. Flowering Plants of The Western Ghats. Jawaharlal Nehru Tropical Botanucal Garden and Research Institute. Vol 2.
Obiajunwa, E.I., Adebajo, A.C., Omobuwajo, O.R., 2002. Essential and trace element contents of some Nigerian medicinal plants. J. Radioanal. Nucl. Chem. 252(3), 473-476.
Oudhia, P., 2001. My Experience with Wonder Crop Safed Musli. Souvenir of International Seminar on Medicinal Plants and Standardization, VHERDS, Chennai, India, pp. 9-10.
Panda, S.K., Das, D., Tripathy, N.K., 2010. Pharmacognostical studies on root tubers of Chlorophytum borivilianum Santapau & Fernandes. Int. J. Pharm. Phytochem. Res. 2(4), 13-17.
Pandey M., Abidi A.B., Singh S., Singh, R.P., 2006. Nutritional evaluation of leafy vegetable Paratha. J. Hum. Ecol. 19(2), 155-156.
Parveen F., Singh M.P., 2018. Adulteration and its mitigation by phytochemical and anatomical screening of family Liliaceae, IOSR. J. Biotechnol. Biochem. 4(2), 22-28.
Patil, S.G., Patil, V.P., 1987. Stomatal studies in the genus Chlorophytum and their taxonomic significance. Phytomorphology 37(2-3), 155-158.
Patil, S.M., Chandanshive, V.V., Tamboli, A.S., Adsul, A.A., Yadav, S.R., Govindwar, S.P., 2015. Analysis of genetic variability in endemic medicinal plants of genus Chlorophytum from the Indian subcontinent using amplified fragment length polymorphism marker. C. R. Biol. 338(12), 838-845.
Patil, S.M., Rane, N.R., Adsul, A.A., Gholave, A.R., Yadav, S.R., Jadhav, J.P., Govindwar, S.P., 2016. Study of molecular genetic diversity and evolutionary history of medicinally important endangered genus Chlorophytum using DNA barcodes. Biochem. Syst. Ecol. 65, 245-252.
Patil, V.N., Abyari, M., Deokule, S.S., 2010. Phramacognostic and Phytochemical evaluation of Chlorophytum glaucum Dalz. - A medicinally important plant. Int. J. Res. Ayurveda Pharm. 2(4), 1297-1302.
Patil, V.N., Abyari, M., Deokule, S.S., 2011. Pharmacognostic and phytochemical study of Chlorophytum laxum L. Br. Int. Res. J. Pharm. 2(6), 169-175.
Patil, V.N., Deokule, S.S., 2010. Pharmacognostic and phytochemical investigation of Chlorophytum breviscapum Dalz. Int. J. Curr. Res. 2(12), 11-24.
Patil, V.N., Deokule, S.S., 2010. Pharmacognostic standardization of Chlorophytum bharuchae Ansari, Ragh., & Hem. Int. J. Curr. Res. 3, 27-32.
Patil, V.N., Deokule, S.S., 2011. Pharmacognostic study of Chlorophytum tuberosum Baker. Int. J. Ayurveda Res. 1(4), 237-242.
Patil, V.N., Somkuwar, S.R., Kabnoorkar, P.S., Deokule, S.S., 2015. Comparative pharmacognostic study of Chlorophytum glaucum Dalz. and Chlorophytum breviscapum Dalz. Int. J. Res. Biosci. Agric. Technol. A6, 97-100.
Phurailatpam, A.K., Geetha, K.A., Gajbhiye, N.A., Saravanan, R., Satyabrata, M., 2009. Comparative study of Chlorophytum borivilianum Sant. & Fernand. and C. arundinaceum Baker - Two ‘Safed Musli’ Species used as vital tonics in Indian system of medicine. Phytomorphology 56(1-2), 77-81.
Poulsen, A.D., Nordal, I., 2005. A phenetic analysis and revision of Guineo-Congolean rain forest taxa of Chlorophytum (Anthericaeae). Bot. J. Linn. Soc. 148(1), 1-20.
Pourmohammad, A., 2013. Application of molecular markers in the medicinal plant studies. Acta Univ. Sapientiae. Agric. Environ. 5, 80-90.
POWO, (2022). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet; http://www.plantsoftheworldonline.org/ Retrieved 21 February 2022.
Prabhu Kumar, K.M., Adsul, A.A., Mathew, J., Balachandran, I., 2016. Nomenclatural notes on the status of the name Chlorophytum clivorum (Asparagaceae). Phytotaxa 243(2), 199-200.
Prabhu Kumar, K.M., Vadakkoot, S.H., Adsul, A.A., Kollencherri, P.V., Balachandran, I., Yadav, S.R., 2014. A new species of Chlorophytum (Asparagaceae) from southern Western Ghats of India. Phytotaxa 188(5), 282-286.
Purohit, S.D., Dev, A., Kukada, G., 1994. Micropropagation of Safed Musali (Chlorophytum borivilianum) a rare Indian Medicinal herb. Plant Cell Tissue Organ Cult. 39(1), 93-96.
Rachchh, M.A., Shah, M.B., Santani, D.D., Goswami, S.S., 2004. Study of Chlorophytum arundinaceum against experimental Gastric Ulcer. Pharm. Biol. 42(8), 592-598.
Raghavan, R.S., Ansari, M.Y., Kamble, Y.S., 1977. Cytotaxonomic studies in Chlorophytum Ker-Gawl. Curr. Sci. 46, 534-535.
Rajua, G.J.N., Saritaa, P., Murtya, G.A.V.R., Kumara, M.R., Reddya, B.S., Charlesa, M.J., Lakshminarayanaa, S., Reddya, T.S., Reddya, S.B., Vijayanb, V., 2006. Estimation of trace elements in some anti-diabetic medicinal plants using PIXE technique. Appl. Radiat. Isot. 64(8), 893-900.
Rajurkar, N.S., Pardeshi, B.M., 1997. Analysis of some herbal plants from India used in the control of Diabetes by NAA and ASS techniques, Appl. Radiat. Isot. 48, 1059.
Remya, V., Ansary, P.Y., Sara Monsy Oommen, 2019. Pharmacognostical analysis of marketsamples of musali from kerala and comparative pharmacological aspects of two varieties of musali, sweta (Chlorophytum borivilianum, Asparagus adscendens) and Krishna (Curculigo orchiodes). Int. J. Ayurveda Res. 3(2), 1502-1507.
Robi, A.J., Prabhu Kumar, K.M., Adsul, A.A., Hareesh, V.S., 2015. Taxonomic notes on the occurrence of Chlorophytum heynei Baker (Asparagaceae) in Western Ghats of India. Devagiri. J. Sci. 1(1), 78-81.
Samantaray, S., Maiti, S., 2011. Factors influencing rapid clonal propagation of Chlorophytum arundinaceum (Liliales: Liliaceae): an endangered medicinal plant. Int. J. Tropic. Biol. Conserv. 59(1), 435-445.
Samantaray, S., Patel, T.K., Geetha, K.A., Maiti, S., 2011. Identification and assessment of genetic relationships in three Chlorophytum species and two high yielding genotypes of C. borivilianum through RAPD markers. Biologia 66(2), 244-250.
Santapau, H., Fernandes, R.R., 1955. A new species of Chlorophytum from Salsette Island. J. Bom. Nat. Hist. Soc. 52, 897.
Sardesai, M.M., Gaikwad, S.P., Yadav, S.R., 2006. A new species of Chlorophytum (Anthericaceae) from Western Ghats, India. Kew Bull. 61(2), 269-271.
Shaanker, R.U., Ganeshaiah, K.N., Rao, M.N., Aravind, N.A., 2004. Ecological consequences of forest use: from genes to ecosystem ˗ a case study in the Biligiri Rangaswamy temple wildlife sanctuary, South India. Conserv. Soc. 2(2), 347-363.
Shaikh, M., Tiwari, A.P., Chandore, A.N., 2017. A new species of Chlorophytum (Asparagaceae) from Madhya Pradesh, India. Phytotaxa 291(2), 159-162.
Sharma, R., Thakur, G.S., Sanodiya, B.S., Pandey, M., Bisen, P.S., 2012. Saponin: A Wonder Drug from Chlorophytum Species. Glob. J. Res. Med. Plants Indigen. Med. 1(10), 503-515.
Sheriff, A., Chennaveeraiah, M., 1975. Cytological studies in Chlorophytum attenuatum complex and cytotaxonomic considerations. Cytologia 40(2), 401-408.
Shinde, S.S., Patil, S.M., Rane, N.R., Adsul, A.A., Gholve, A.R., Pawar, P.K., Yadav, S.R., Govindwar, S.P., 2016. Comprehensive investigation of free radical quenching potential, total phenol, flavonoid and saponin content, and chemical profiles of twelve Chlorophytum Ker Gawl. Species. Indian J. Nat. Prod. Resour. 7(2), 125-134.
Sidapara, M.G., Raviya, J.R., Pandya, D.J., 2013. Establishment of quality parameters to detect substitution of Shatavari by Safed musali. Int. J. Pharma. Pharm. Sci. 6(1), 742-746.
Singh, A., Khanuja, S.P.S., Singh, S., 2003. Agronomic practices for the production of Safed musli (Chlorophytum borivilianum Santapau & Fernandes) in India. Nat. Prod. Radiance 2(6), 308-313.
Sriram, S., Meenaa, V., Kavitha Srilakshmi, J., 2012. Evaluation of antibacterial activity of Chlorophytum borivilianum against selected pathogens. J. Res. Antimicrob. 1, 028-031.
Sundaram, S., Dwivedi, P., Purwar, S., 2011. Antibacterial Activities of Crude Extracts of Chlorophytum borivilianum to Bacterial Pathogens. Res. J. Med. Plants 5(3), 343-347.
Swarnkar, S., Katewa, S.S., 2008. Ethnobotanical observation on tuberous plants from tribal area of Rajasthan (India). Ethnobot. Leaflets 12, 647-66.
Tandon, M., Shukla, Y.N., 1992. Saponins from Asparagus adscendens and Chlorophytum arundinaceum. J. Indian Chem. Soc. 69, 893.
Tandon, M., Shukla, Y.N., 1996. Arundinoside B, a new acylated glucoside from Chlorophytum arundinaceum. J. Indian Chem. Soc. 35, 988-989.
Tandon, M., Shukla, Y.N., 1997. Arundinoside A a new spirostane from Chlorophytum arundinaceum. J. Indian Chem. Soc. 74, 56-58.
Tandon, M., Shukla, Y.N., Thakur, R.S., 1992. 4-Hydroxy-8,11-oxidoheneicosanol and other constituents from Chlorophytum arundinaceum roots. Phytochemistry 31(7), 2525-2526.
Tarafder, C.R., 1983. Ethnogynecology in relation to plants: 2. Plants used for abortion. J. Econ. Taxon. Bot. 4(2), 507-516.
The Wealth of India, 1950. Raw materials, Published by PID Council of Scientific and Industrial Research, New Delhi. 3, pp. 482-483.
The Wealth of India—A Dictionary of Indian Raw Material and Industrial Products, 2000. First Supplement Series (Raw material) National Institute of Science Communication, CSIR, New Delhi. 1: A-Ci, pp. 247.
Tripathi, N., Saini, N., Nair, P., Tiwari, S., 2012. Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers. Physiol. Mol. Biol. Plants. 18(2), 161-167.
Valya, G., Ragan, A., Raju, V.S., 2009. In vitro antimicrobial study of root extract of Chlorophytum arundinaceum Baker. Nat. Prod. Radiance 8(5), 503-506.
Venditti, A., 2020. What is and what should never be: artifacts, improbable phytochemicals, contaminants and natural products. Nat. Prod. Res. 34(7), 1014-1031.
Vijay, L., Pandey, K., Roy, R., Joshi, S.B., Kunnath, P.M., Chandra, R., Srivastava, A.K., Raina, D., Rastogi, A.K., 2007. Isolation of Tigogenin Pentaglycoside from Chlorophytum nimmonii, Patent No. US 7,160,866 B2.
Vijayan, A., Liju, V.B., Reena John, J.V. Parthipan, B., Renuka, C., 2007. Traditional remedies of Kani tribes of Kottor reserve forest, Agasthayavanam, Thiruvananthapuram, Kerala. Indian J. Trad. Knowl. 6(4), 589-594.
Willis, J.C., 1973. A Dictionary of the Flowering Plants and Ferns. 8th Ed. Cambridge: Cambridge University Press.
Wolverton, B.C., McDonald, R.C., Watkins, J.E.A., 1984. Foliage plants for removing indoor air pollutants from energy-efficient homes. Econ. Bot. 38(2), 224-228.
World Checklist of Selected Plant Families wcsp.science.kew.org Search for "Chlorophytum comosum" The Board of Trustees of the Royal Botanic Gardens, Kew. Accessed 20th Oct 2017.