Phytochemical constituents found in Physalis peruviana L. leaf extracts and their ability to inhibit alpha-glucosidase and scavenge DPPH free radicals in vitro

Subject Areas : Pharmacognosy

Félicien  Kasali ¹ (Pharm-Bio Technology and Traditional Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda|Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda|Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, PO Box 570 Bukavu, the Democratic Republic of Congo)
Muhammad  Ali ² (HEJ Research Institute of Chemistry, International Centre for Chemical & Biological Sciences, University of Karachi, Karachi-75270, Pakistan)
Jonans  Tusiimire ³ (Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda)
Mehreen  Lateef ⁴ (Multi-Disciplinary Research Lab, Bahria University, Medical & Dental College, Karachi, Pakistan)
Ruth  Omole ⁵ (School of Chemistry and Material Science, Technical University of Kenya, P.O Box 52428- 00200, Nairobi, Kenya)
Justin  Kadima ⁶ (Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, PO Box 570 Bukavu, the Democratic Republic of Congo|Department of Pharmacology, School of Medicine and Pharmacy, University of Rwanda, PO Box 117 Huye, Rwanda Mbarara)
Amon  Agaba ⁷ (Department of Pharmacology and Therapeutics, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda)

Received: 2021-11-29 Accepted : 2022-02-28 Published : 2022-05-01

Keywords: GC-FID, Hexane fraction, MS, Bio-compounds, <i>Physalis peruviana</i> L,

Abstract :

Physalis peruviana L. is widely used in folk medicines to manage diabetes. This study aimed to identify new chemical constituents and explore in vitro antioxidant ability and alpha-glucosidase inhibition activity to explain its antidiabetic mechanism. Methanolic extracts and hexane fractions were prepared and analyzed by CG-FID and GC-MS. In vitro antidiabetic activity was assessed on &alpha;-glucosidase inhibition and DPPH models. The phytochemical analysis detected 29 constituents, manly heptacosane (25) and hexadecanoic acid methyl ester (14). Aliphatic hydrocarbons and esters were identified as the main classes of secondary metabolites. Compared to the standard, the methanol-water fraction was the most potent fraction on &alpha;-glucosidase inhibition (IC50 32.5 &plusmn; 0.27 &micro;g/mL). The antioxidant values (IC50) of two fractions, and the reference were quite close (44.5 &plusmn; 0.54; 49.5 &plusmn; 0.87; 44.2 &plusmn; 0.77 &micro;g/mL). Almost 82.75% are recognized for the first time in the leaf hexane fractions of this plant. The observed antioxidant activity was found to be significantly notable.

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