تغییرات بیان ژن سرکوبگر تومور (TP53) بافت کبد و شاخص های اکسیداتیو متعاقب شش هفته تمرین مقاومتی و مصرف عصاره آناناس در موش های مبتلا به سرطان ملانوما
محورهای موضوعی : تشخیص مولکولی نشانگر های بیوشیمیایی و ژنتیکی
سعید شهرستانی
1
,
حسین عابدنطنزی
2
*
,
بهرام عابدی
3
,
شهرام سهیلی
4
1 - 1- دانشجوی دکتری تخصصی فیزیولوژی ورزشی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، گروه تخصصی تربیت بدنی و علوم ورزشی، تهران، ایران
2 - گروه تخصصی تربیت بدنی و علوم ورزشی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 - Department of Sports Sciences, Islamic Azad University, Mahalat Branch, Mahalat, Iran
4 - دانشگاه آزاد اسلامی واحد شهرقدس. گروه تربیت بدنی
کلید واژه: تمرین مقاومتی, ¬آناناس , سرطان ملانوما, تومور, P53,
چکیده مقاله :
زمینه و هدف: سرطان ملانوما شدیدترین زیر مجموعه سرطان پوست است که قدرت تهاجم بالا و متاستاز سريع به ساير اندامها را دارد. اخیرا نقش تمرینات ورزشی در پیشگیری و درمان سرطان بسیار مورد توجه قرار دارد . آناناس نیز متعلق به خانواده برومولياسه و زير خانواده برومولويده مي باشد که خواص ضد سرطانی آن مطرح شده است. ژنP53 یک ژن مهار کننده تومور است. هدف پژوهش حاضر مطالعه تاثیر شش هفته تمرین مقاومتی و مصرف عصاره آناناس بر حجم تومور و بیان ژن P53 بافت تومورو عوامل اکسیدانی آنتی اکسیدانی در موش های مبتلا به سرطان ملانوما بود.
مواد و روش ها: این مطالعه بنیادی بر روی 32 موش نژاد C57 بعنوان نمونه شروع شد که در انتها بر روی 20 سر موش های باقیمانده در چهار گروه شامل کنترل، تمرین مقاومتی، عصاره آناناس و تمرین مقاومتی- آناناس انجام شد. پس از القای تومور به موش ها ، برنامه تمرین مقاومتی و عصاره دهی آناناس به میزان mg/kg 300 بصورت گاواژ به مدت شش هفته اجرا شد. وزن و حجم تومور موش ها اندازه گیری شد. اندازه گیری بیان ژن P53 بافت تومور به روش RT-PCR انجام گرفت. سپس داده ها با استفاده آنالیز واریانس یک طرفه، آزمون تحلیل واریانس دو عاملی و تعقیبی مورد تجزیه تحلیل قرار گرفتند. سطح معنی داری p≤0.05 در نظر گرفته شد.
نتایج : نشان داد در مقایسه با گروه کنترل، تمرین مقاومتی و تمرین - آناناس به کاهش معنیدار حجم تومور و کاهش بیان ژن P53 بافت تومور و افزایش شاخص آنتی اکسیدانی GPXو کاهش شاخص استرس اکسیداتیو MDA در گر.ههای تجربی شد که در گروه تعاملی تغییرات میانگین و اثر بخشی بیشتر بود .
نتیجه گیری: تمرین مقاومتی و عصاره آناناس منجر به کاهش وزن وکاهش معنی‌دارحجم تومور و افزایش بیان ژن p53 سرکوب‌کننده تومور و افزایش شاخص آنتی اکسیدانی GPXو کاهش شاخص استرس اکسیداتیو MDA در در گروه‌های تجربی نسبت به گروه کنترل در بافت تومور شد.
Background & Aim: Melanoma cancer is the most severe subtype of skin cancer, which has high invasiveness and rapid metastasis to other organs. Recently, the role of sports training in the prevention and treatment of cancer has received much attention. Pineapple also belongs to the bromolyase family and the bromoloid subfamily, whose anti-cancer properties have been suggested. P53 gene is a tumor suppressor gene. The aim of the present study was to study the effect of six weeks of resistance training and consumption of pineapple extract on tumor volume and tumor tissue P53 gene expression in mice with melanoma cancer.
Materials & Methods: The basic study began with 32 C57 mice as a sample, which was eventually divided into four groups, including the remaining 20 mice in four groups including control, resistance training, pineapple extract and resistance training-pineapple. After tumor induction in mice, the program of resistance training and pineapple extract at the rate of 300 mg/kg was implemented for six weeks. The weight and tumor volume of mice were measured. Measurement of P53 gene expression in tumor tissue was done by RT-PCR method. Then the data were analyzed using one-way analysis of variance, two-factor analysis of variance and post hoc test. A significance level of p≤0.05 was considered.
Results: Results was shown that compared to the control group, resistance training and pineapple-based training significantly reduced tumor volume and decreased P53 gene expression in tumor tissue and increased GPX antioxidant index and decreased MDA oxidative stress index in experimental groups, while in the interaction group, the mean changes and effectiveness were greater.
Conclusion: Resistance training and pineapple extract led to weight loss and significant reduction in tumor volume and increased expression of the tumor suppressor p53 gene and increased GPX antioxidant index and decreased MDA oxidative stress index in experimental groups compared to the control group in tumor tissue.
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