تعیین الگوی فنوتیپی و ژنوتیپی مقاومت آنتی‌بیوتیکی در ایزوله های اشریشیا کلی یوروپاتوژن جداشده از بیماران دیابتی در شهرستان شهرکرد
محورهای موضوعی : میکروبیولوژیفاطمه خداوردی پور 1 , امین روزبهی 2
1 - گروه میکروبیولوژی، دانشکده علوم،واحد شهرکرد، دانشگاه آزاد اسلامی،شهرکرد، ایران
2 - گروه میکروبیولوژی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامي، شهرکرد، ايران
کلید واژه: اشریشیا کلی یوروپاتوژنیک, الگوی فنوتیپی, بیماران دیابتی, مقاومت آنتی‌بیوتیکی,
چکیده مقاله :
مقدمه : جلوگيري از انتشار مقاومت‌هاي دارويي يكي از مسائل مهم در جامعه است. اشریشیا کلی یکی از شایع ترین عوامل باکتریایی جداشده از عفونت‌های ادراری و بیمارستانی می‌باشد. درمان عفونت‌های ناشی از آن بدلیل کسب ژن‌های مقاومت مشکل است. هدف از این مطالعه بررسی الگوی فنوتیپی و ژنوتیپی مقاومت آنتی‌بیوتیکی در سویه‌های بالینی اوروپاتوژنیک جداشده از بیماران دیابتی بود. مواد و روش‌ها: در این مطالعه 51 ایزوله بالینی اشریشیا کلی از بیماران دیابتی مراجعه‌کننده به آزمایشگاه‌های تشخیص طبی موردبررسی قرار گرفت. تایید ایزوله ها با استفاده از روشهای بیوشیمیایی و مولکولی بر اساس ردیابی ژن 16srRNA و مقاومت آنتی‌بیوتیکی ایزوله‌ها با روش دیسک دیفیوژن و بررسی مولکولی ژن¬های مقاومت (qnr،tet A، tet B, aac (3)IIa و sul1 ) انجام شد. یافته¬ها: بیشترین مقاومت نسبت به آمپی سیلین (66/66 درصد) و کمترین مقاومت نسبت به نیتروفورانتئین (96/1درصد) گزارش گردید. فراوانی ژن‌های tet A، tet B،qnr ، sul 1 و aac (3)IIa به ترتیب 62/68 ، 7/64، 41/29، 21/39و 41/29 درصد گزارش گردید. در تجزیه و تحلیل آماری بین مقاومت به آنتی‌بیوتیک تتراسایکلین و ژن‌های tet A، tet B ارتباط آماری معنی دار مشاهده گردید. بحث: تشخيص به موقع سويه‌هاي مقاوم در انتخاب درمان مناسب و جلوگيري از گسترش مقاومت، ضروري است. پیشنهاد می‌شود،بدلیل اهمیت درمان عفونت‌هاي ادراري، درمان با توجه به الگوي حساسیت و مقاومت منطقه صورت گیرد تا از ایجاد مقاومت دارویی و شکست‌هاي درماني که منجر به عارضه دارشدن عفونت می‌گردد، جلوگیري شود.
Introduction: Preventing the spread of drug resistance is one of the most important issues in society. Escherichia coli is one of the most common bacterial agents isolated from the urinary tract and nosocomial infections. Treatment of infections due to it is difficult due to the acquisition of resistance genes. This study aimed to investigate the phenotypic and genotypic pattern of antibiotic resistance in clinical uropathogenic strains isolated from diabetic patients. Materials and methods: A total of 51 E. coli isolates from urinary tract infection in diabetic patients obtained from clinical, were used in this study. Isolates were confirmed by chemical tests and molecular techniques based on tracking of the 16srRNA gene. Antimicrobial resistance assessment of isolates was done using molecular methods base on (qnrA, tet A, tet B, aac (3)IIa, sul1) and disk diffusion. Results: Most resistance r to ampicillin (66.66%) and the lowest resistance to Nitrofurantoin (1.96%) were reported. The frequency of tet A, tet B, qnr A, sul 1 and aac (3) IIa genes reported 68.62%, 64.7%, 29.41%, 39.21%, and 29.41% respectively. The statistical analysis shows a significant relationship between resistance to the antibiotic tetracycline and the gene tet A, tet B statistically. Conclusion: Early detection of resistant strains to select the most appropriate treatment options is essential to prevent the spread of resistance. It is suggested, as treatment for urinary tract infections is important, so to prevent drug resistance and treatment failure, it should be done according to the resistance pattern in the region.
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