Cloning and phylogenetic analysis of Bovine immunodeficiency virus gag gene in Iranian isolates
محورهای موضوعی : میکروبیولوژی
1 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد شهرکرد
کلید واژه: Bovine immunodeficiency virus, gag gene, pTZ57R/T vector, pGEX-4T-3 vector, Phylogenetic analysis,
چکیده مقاله :
ویروس نقص ایمنی گاوی عضوی از خانواده رتروویریده، جنس لنتی ویروس، دارای سه ژن مهم به نام‌های gag، pol و env است محصول PCR ژن gag ویروس نقص ایمنی گاوی جدا شده از مناطق مختلف ایران، در ناقل pTZ57R/T کلون شد؛ سپس قطعات الحاقی توسط آنزیم‌های محدودکننده BamHI و XhoI هضم و سپس در ناقل بیانی pGEX-4T-3 کلون شدند. تجزیه و تحلیل توالی‌های ژن gag ویروس نقص ایمنی گاوی به دست آمده از قطعه الحاقی انجام شد. توالی gag ویروس نقص ایمنی گاوی ایرانی با 5 توالی متناظر دیگر از BIV جدا شده در کشورهای مختلف مقایسه شد. در نهایت، مشخص شد که تجزیه و تحلیل نوکلئوتیدی توالی‌ها، تنوعی بین 4/0 تا 6/10 درصد را نشان می‌دهد و رسم درخت فیلوژنتیکی دو خوشه را در آن آشکار کرد.
Bovine immunodeficiency virus (BIV), a member of the family Retroviridae, genus Lentivirus, has three important genes namely gag, pol and env. In the first step, PCR product of gag gene of BIV, isolated in different regions of Iran, were cloned in to a pTZ57R/T vector; then insert were digested by BamHI and XhoI restriction enzymes and then cloned in to pGEX-4T-3 as an expression vector. Analysis of the partial bovine immunodeficiency virus (BIV) gag gene sequences obtained from insert and was run. The Iranian BIV gag sequence was compared to 5 other corresponding sequences of BIV isolated in different countries. In the end, It was revealed that nucleotide analysis of the sequences witnessed, a variation of 0.4-10.6% and constructing phylogenetic tree revealed two clusters in it.
1. Van der Maaten MJ, Boothe AD, Seger CL. Isolation of a virus from cattle with persistent lymphocytosis. J Natl Cancer Inst. 1972; 49: 1649-1657.
2. Snider TG, Hoyt PG, Jenny BF, Coats KS, Luther DG, Storts RW, Battles JK, Gonda MA. Natural and experimental bovine immunodeficiency virus infection in cattle. Vet Clin North Am Food Anim Prac. 1997; 13:151-176.
3. Snider TG 3rd, Luther DG, Jenny BF, Hoyt PG, Battles JK, Ennis WH, Balady J, Blas-Machado U, Lemarchand TX, Gonda MA. Encephalytis , lymphoid tissue depletion and secondary disease associated with bovine imunodeficiency virus in a dairy herd. Comp Immunol Microbiol Infect Dis.1996; 19:117-131.
4. Gonda MA, Braun MJ, Carter SG, Kost TA, Bess JW Jr, Arthur LO, Van der Maaten MJ.,. Characterization and molecular cloning of a bovine lentivirus related to the human immunodeficiency virus. Nature. 1987; 330: 388–391.
5. Garvey KJ, Oberste MS, Elser JE, Braun MJ, Gonda MA. Nucleotide sequence and genome organization of biologically active proviruses of the bovine immunodeficiency-like virus.Virology. 1990; 175: 391-409.
6. Oberste MS, Gonda MA). Conservation of amino-acid sequence motifs in lentivirus Vif proteins.Virus genes.1992; 6: 95-102.
7. Lechner F, Machado J, Bertoni G, Seow HF, Dobbelaere DA, Peterhans E. Caprine arthritis encephalitis virus dysregulates the expression of cytokines in macrophages. J Virol . 1997; 71: 7488–7497.
8. Turelli P, Guiguen F, Mornex JF, Vigne R, Quérat G. dUTPase- minus caprine arthritis-encephalitis virus is attenuated for pathogenesis and accumulates G-to-A substitutions . J Virol. 1997; 71: 4522–4530.
9. Chen H, Wilcox G, Kertayadnya G, Wood C). Characterizations of the Jembrana disease virus tat gene and the cis-and trans-regulatory elements in its long terminal repeats. J Virol . 1999; 73: 658–666.
10. Agnarsdóttir G, Thorsteinsdóttir H, Oskarsson T, Matthíasdóttir S, Haflidadóttir BS, Andrésson OS, Andrésdóttir V. The long terminal repeat is a determinant of cell tropism of maedi-visna virus. J Gen Virol. 2000; 81: 1901–1905.
11. Gonda MA, Luther DG, Fong SE, Tobin GJ. Bovine immunodeficiency virus: molecular biology and virus-host interactions. Virus Res. 1994; 32:155–181.
12. St-Louis MC, Cojocariu M, Archambault D. The molecular biology of bovine immunodeficiency virus: a comparison with other lentiviruses. Anim Health Res Rev. 2004; 5: 125–143.
13. Rasmussen L, Battles JK, Ennis WH, Nagashima K, Gonda MA. Characterization of virus-like particles produced by a recombinant baculovirus containing the gag gene of the bovine immunodeficiency-like virus. Virology. 1990; 178: 435–451.
14. Zhang S, Xue W, Wood C, Chen Q, Kapil S, Minocha HC. Detection of bovine immunodeficiency virus antibodies in cattle by western blot assay with recombinant gag protein. J Vet Diagn Inves. 1997; 9:347-351.
15. Battles JK , Hu MY, Rasmussen L, Tobin GJ, Gonda MA. Immunological characterization of the gag gene products of bovine immunodeficiency virus .J Virol. 1992; 66: 6868–6877.
16. Tobin GJ, Sowder RC 2nd, Fabris D, Hu MY, Battles JK, Fenselau C, Henderson LE, Gonda MA. Amino acid sequence analysis of the proteolytic cleavage products of the bovine immunodeficiency virus Gag precursor polypeptide. J Virol. 1994; 68: 7620–7627.
17. Lillehoj EP, Salazar FH, Mervis RJ, Raum MG, Chan HW, Ahmad N, Venkatesan S. Purification and structural characterization of the putative gag-pol protease of human immunodeficiency virus. J Virol. 1998; 62: 3053-3058.