اثر هشت هفته مصرف عصاره کروستين همراه با تمرین هوازی بر بیان ژن VEGF و اندوتلین -1 بافت قلب موش‌های ديابتی سالمند
محورهای موضوعی : نقش بیان ژن در سلامتی
شهرام پویا
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خسرو جلالی دهکردی
2
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فرزانه تقیان
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مهدی کارگرفرد
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سید علی حسینی
5
1 - گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان(خوراسگان)، اصفهان، ایران
2 - گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان(خوراسگان)، اصفهان، ایران
3 - گروه فیزیولوژی ورزش، دانشگاه آزاد اسلامی، واحد اصفهان(خوراسگان)، اصفهان، ایران
4 - استاد،گروه فیزیولوژی دانشکده علوم ورزشی،دانشگاه اصفهان، اصفهان، ایران
5 - گروه فیزیولوژی ورزش، واحد مرودشت، دانشگاه آزاد اسلامی، مرودشت، ایران
کلید واژه: تمرین, کروستین, آنژیوژنز, قلب, سالمند, , دیابت ,
چکیده مقاله :
زمينه و هدف: دیابت از عوامل خطر اصلی بیماری‌های قلبی عروقی هستند که منجر به اختلال در عملکرد اندوتلیال و رگزایی معیوب می‌شوند .مطالعه حاضر با هدف بررسی اثر هشت هفته مصرف عصاره کروستين همراه با تمرین هوازی بر بیان ژن VEGF و اندوتلین -1 بافت قلب موش‌های پيش ديابتی و ديابتی سالمند انجام گرفت روش بررسی: در این کارآزمایی تجربی، 25 سر موش نر c57bl6 ماده سالمند مبتلا به دیابت با سن 14-16 هفته و وزن 30-35 گرم به گروه¬های ((1)دیابتی (2)دیابتی+تمرین هوازی(3)دیابتی+کروستین(4)دیابتی+تمرین هوازی+کروستین و(5)کنترل سالم تقسیم شدند.. گروه¬ه تمرین هوازی به مدت هشت هفته، پنج جلسه در هفته تمرین هوازی تناوبی انجام دادند و دوز mg/kg 40 استروپتوزتوسین (STZ) تزریق شد. همچنین کروستین را mg/kg/day 30 به صورت تزریق صفاقی دریافت کردند.م.جهت تجزیه و تحلیل داده¬ها از آزمون آنالیز واریانس یک¬راهه و آزمون تعقیبی توکی استفاده شد (05/0≥P). يافته¬ها: مقادیر بیان¬ژنی VEGF و اندوتلین 1 در گروه دیابت به طور معنی داری بالاتر از سایر گروهها بود (05/0≥P)؛ مقادیر بیان ژنی VEGF و اندوتلین 1 در گروه دیابتی+تمرین هوازی و دیابتی+کروستین و دیابتی+تمرین هوازی+کروستین به طور معنی داری پایین تر از گروه دیابتی بود (05/0≥P). نتيجه¬گيری: به نظر می¬رسد هوازی تناوبی و کروستین هم به تنهایی و هم به طور همزمان در بهبود آنژیوژنز در بافت قلب مبتلا به دیابت موثر باشند. لذا استفاده از هوازی تناوبی و کروستین در شرایط دیابتی سالمندی توصیه می¬شود.
Background and aims: Diabetes is one of the main risk factors for cardiovascular diseases that lead to impaired endothelial function and defective angiogenesis. The purpose of this study is to investigate the effect of eight weeks of consumption of crocetin extract along with aerobic exercise on the expression of VEGF and endothelin-1 gene in the heart tissue of preterm rats. Diabetes and elderly diabetes were performed Methods In this experimental trial, 25 elderly female c57bl6 male mice with diabetes, aged 14-16 weeks and weighing 30-35 grams, were divided into groups (1) diabetic (2) diabetic + aerobic exercise (3) diabetic + crocetin ( 4) Diabetics + aerobic exercise + crocetin and (5) healthy control were divided. Aerobic exercise group did intermittent aerobic exercise for eight weeks, five sessions a week, and 40 mg/kg dose of streptozotocin (STZ) was injected. They also received 30 mg/kg/day crocetin. The expression of VEGF and endothelin 1 genes was measured by Real Time PCR technique. One-way analysis of variance and Tukey's post hoc test were used for data analysis. was (P≥0.05). Results: The expression values of VEGF and endothelin 1 in the diabetes group were significantly higher than other groups (P≥0.05); Conclusion: It seems that interval aerobics and crocetin both alone and simultaneously are effective in improving angiogenesis in diabetic heart tissue. Therefore, it is recommended to use periodic aerobics and crocetin in diabetic conditions of old age
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