تاثیر هشت هفته تمرین مقاومتی در ترکیب با مکمل BCAA نانولیپوزوم بر بیان ژن های HSP60 و HSP70 میتوکندری کاردیومیوسیت های رتهای مدل سکته قلبی
محورهای موضوعی : نقش بیان ژن در سلامتی
الهام فرهادفر
1
,
ماکان خواجه وندی
2
1 - گروه تربیت بدنی و علوم ورزشی، دانشگاه آزاد اسلامی، واحد دزفول، دزفول
2 - گروه تربیت بدنی و علوم ورزشی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: تمرین مقاومتی, مکمل BCAA نانولیپوزوم, HSP60 و HSP70 و رتهای مدل سکته قلبی.,
چکیده مقاله :
هدف از پژوهش حاضر بررسی تاثیر هشت هفته تمرین مقاومتی در ترکیب با مکمل BCAA نانولیپوزوم بر بیان ژنهای HSP60 و HSP70 میتوکندری کاردیومیوسیتهای رتهای مدل سکته قلبی بود. در اين مطالعه تجربي، 32 سر موش صحرایی نر نژاد ویستار مدل سکته قلبی به صورت تصادفی به 4 گروه کنترل، مکمل (BCAA نانولیپوزوم)، توام (تمرین مقاومتی+ مکمل BCAAنانولیپوزوم) و تمرین (تمرین مقاومتی) تقسیم شدند. تمرين مقاومتي شامل هشت هفته تمرين نردبان با شدت متوسط (70 درصد از MVCC) و پنج روز در هفته بود. در گروه¬های مکمل و توام 5 روز در هفته و به مدت 8 هفته، مکمل BCAA نانولیپوزوم و به میزان 600 میلی گرم به ازای هر کیلوگرم وزن بدن به صورت گاواژ دریافت شد. بیان نسبی ژنهای HSP60 و HSP70 میتوکندری با استفاده از روش Real-time PCR به دست آمد. ﺩﺍﺩﻩ ﻫﺎ ﺑﺎ ﺍﺳﺘﻔﺎﺩ ﺍﺯ ﺁﺯﻣﻮن ﺁﻣﺎری تحلیل واریانس دوطرفه ﻭ ﺁﺯﻣﻮﻥ تعقیبی توکیﺁﻧﺎﻟﻴﺰ ﺷﺪﻧﺪ. كليه عمليات آماري توسط نرم افزار(SPSS) نسخه 23 و سطح معني داري آزمون ها در سطح 05/0≥p مورد تجزیه و تحلیل قرار گرفتند.نتایج آزمون نشان داد که ميانگين بیان ژنهای HSP60 و HSP70 کاردیومیوسیت‌ها در گروه‌های تمرين کرده با و بدون مصرف BCAA (تمرین+ مکمل و تمرین) به طور معنی‌داری بیشتر از گروه‌های تمرين نکرده با و بدون مصرف BCAA (مکمل و کنترل) است. نتایج تحقيق اهميت HSP60 و HSP70 را در تكثير و تمایز سلول های عضلانی همراه با بازسازی از آسيب ناشی از انفارکتوس قلبی را نشان می دهد.
The purpose of this study was to investigate the effect of eight weeks of resistance training in combination with nanoliposome BCAA supplement on the expression of HSP60 and HSP70 genes in mitochondrial cardiomyocytes of heart attack model rats. 32 male Wistar rats with heart attack model were randomly divided into 4 groups: control, supplemented (BCAA nanoliposome), combined (resistance training + BCAA nanoliposome supplement) and training (resistance training). Resistance training consisted of eight weeks of ladder training with moderate intensity (70% of MVCC) and five days a week. In the supplement and combined groups, 5 days a week and for 8 weeks, BCAA nanoliposome supplement was received by gavage in the amount of 600 mg per kilogram of body weight. The relative expression of mitochondrial HSP60 and HSP70 genes was obtained using Real-time PCR method. The data were analyzed using two-way analysis of variance and Tukey's post hoc test. The results of the two-way analysis of variance test showed that the average expression of HSP60 and HSP70 genes in cardiomyocytes in the trained groups with and without BCAA consumption (exercise + supplement and exercise) was significantly higher than the untrained groups with and without consumption. BCAA (supplement and control. The results of this research clearly show the importance of HSP60 and HSP70 in the proliferation and differentiation of muscle cells along with regeneration from damage caused by heart infarction.
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