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Open Access Article
1 - Isolation and identification of salinity and drought tolerant bacteria from Jiroft agricultural fields
زینب فتوحیانOne of the new ways to cope with salinity and reduce its harmful effects is to introduce salt-tolerant bacteria. In this research, indigenous bacteria resistant to salinity and drought were isolated and identified. The growth rate of isolates at different concentrations MoreOne of the new ways to cope with salinity and reduce its harmful effects is to introduce salt-tolerant bacteria. In this research, indigenous bacteria resistant to salinity and drought were isolated and identified. The growth rate of isolates at different concentrations of salinity (5%, 10%, 15%, 20%, 25%, 35% and 40%), drought(0.50, -0.15, -0.03, -0.49 and -0.73 MPa), pH and 50°C. Then, the production of Auxin, Siderophore, hydrogen cyanide and phosphate solubility were investigated. A total of 42 soil samples were collected, 50 bacteria were obtained. The K4, K10, K12, K14, K15 codes for the Konar-sandal, C18, C10, C11 codes for Karim-abad, A2, A3, A4 codes for Anbar-abad were resistant to salinity. Also, the K4, K14, K15, C8 codes had the ability to grow in water potential at -0.73. The C8 and K4 isolates had the ability to grow at high pH and 50°C. Also, isolate C8 produced the highest amount of Auxin (1.8 μg/ml) and it was the only isolate that dissolved phosphate. The isolates C8 and K14 had the ability to produce Siderophore. Therefore, C8 isolate was Bacillus subtilis as the superior isolate. Manuscript profile -
Open Access Article
2 - Study of application of Biosynthesis of silver nanoparticles using Trachyspermum ammi extract in controlling of Onion bacterial rot
زینب فتوحیانToday, the use of metal nanoparticles, as a suitable alternative to chemical poisons reduces their risk to the environment and consumers. Therefore, the aim of this study is the biosynthesis of silver nanoparticles using the aqueous extract of native Ajowan seeds and it MoreToday, the use of metal nanoparticles, as a suitable alternative to chemical poisons reduces their risk to the environment and consumers. Therefore, the aim of this study is the biosynthesis of silver nanoparticles using the aqueous extract of native Ajowan seeds and its effect against Onion bacterial rot. In this research, first, isolation and identification of pathogenic bacteria from different areas of Jiroft city was done. Then the biosynthesis of Ag NPs was carried out by aqueous extract of Ajowan seeds and the their effects against Pectobacterium were investigated in laboratory and warehouse conditions. A total of 12 bacterial isolates, N3 isolate from Anbarabad region had the highest pathogenicity. The biosynthesis of Ag NPs was confirmed by observing the date color after 15 minutes in the dark at room temperature and the ultraviolet spectrometer showed that the surface plasmon resonance is at 420 nm. The growth inhibition rate of biosynthesized Ag NPs and Mancozeb poison each alone and their combination against Pectobacterium in vitro were 62.45, 100 and 88.67%, respectively. Also, Ag NPs and Mancozeb each alone within three months and combining them together within two months caused 100% shelf life of onions in storage. However, the combination of biosynthesized Ag NPs and Mancozeb together with Pectobacterium had 100% inhibition in the first and second month and 85% in the third month against bacterial rot disease. Therefore, the results showed that biosynthesized Ag NPs were effective in controlling pathogenic bacteria and can replace agricultural toxins. Manuscript profile -
Open Access Article
3 - Isolation and identification of salinity and drought tolerant bacteria from Jiroft agricultural fields
زینب فتوحیان احمد یوسفیDue to the increasing spread of dry lands in different regions of the world, including Iran, it should be adapted to the new conditions and there must be a plan to prevent the development of these lands. Due to the fact that some areas of Jiroft city were affected by dr MoreDue to the increasing spread of dry lands in different regions of the world, including Iran, it should be adapted to the new conditions and there must be a plan to prevent the development of these lands. Due to the fact that some areas of Jiroft city were affected by drought and salinity facoers, therefore they were considered in this study. One of the new ways to cope with salinity in plants and reduce its harmful effects is to introduce salt-tolerant bacteria. In this research, indigenous bacteria resistant to salinity and drought were isolated and identified. The growth rate of isolates at different concentrations of salinity (5%, 10%, 15%, 20%, 25%, 35% and 40%), drought(0.50, -0.15, -0.03, -0.49 and -0.73 MPa), pH and temperature of 50°C was calculated by reading the optical absorption by spectrophotometer. Then, the production of auxin, siderophore and hydrogen cyanide and phosphate solubility of resistant isolates were investigated. A total of 42 soil samples were collected from the salty and dry areas of Jiroft city in the crop year 2019 and of this number of soil amples, 50 bacteria were obtained. From isolates, K4, K10, K12, K14, K15 codes for the Konar-sandal, C18, C10, C11codes for Karim-abad, A2, A3, A4 codes for anbar-abad were resistant to salinity due to their growth up to 40% salt concentration. Also, the K4, K14, K15, and C8 codes had the ability to grow in water potential at -0.73 and resistant to drought. From selective isolates, the isolate C8 had the ability to grow at high pH and C8 and K4 isolates were capable of growing at 50°C. Also, isolate C8 produced the highest amount of auxin among isolates by producing 1.8 μg/ml of auxin and it was the only isolate that dissolved phosphate. The isolates C8 and K14 had the ability to produce cidrofur in isolates. Therefore, according to biochemical and molecular identification of isolate C8, it was Bacillus subtilis as the superior isolate. This isolate was isolated from the Karim-abad region. Manuscript profile -
Open Access Article
4 - Application of Biosynthesis of silver nanoparticles using Trachyspermum ammi extract in controlling of Onion bacterial rot
زینب فتوحیانToday, the use of metal nanoparticles, as a suitable alternative to chemical poisons reduces their risk to the environment and consumers. Therefore, the aim of this study is the biosynthesis of silver nanoparticles using the aqueous extract of native Ajowan seeds and it MoreToday, the use of metal nanoparticles, as a suitable alternative to chemical poisons reduces their risk to the environment and consumers. Therefore, the aim of this study is the biosynthesis of silver nanoparticles using the aqueous extract of native Ajowan seeds and its effect against Onion bacterial rot. In this research, first, isolation and identification of pathogenic bacteria from different areas of Jiroft city was done. Then the biosynthesis of Ag NPs was carried out by aqueous extract of Ajowan seeds and the their effects against Pectobacterium were investigated in laboratory and warehouse conditions. A total of 12 bacterial isolates, N3 isolate from Anbarabad region had the highest pathogenicity. The biosynthesis of Ag NPs was confirmed by observing the date color after 15 minutes in the dark at room temperature and the ultraviolet spectrometer showed that the surface plasmon resonance is at 420 nm. The growth inhibition rate of biosynthesized Ag NPs and Mancozeb poison each alone and their combination against Pectobacterium in vitro were 62.45, 100 and 88.67%, respectively. Also, Ag NPs and Mancozeb each alone within three months and combining them together within two months caused 100% shelf life of onions in storage. However, the combination of biosynthesized Ag NPs and Mancozeb together with Pectobacterium had 100% inhibition in the first and second month and 85% in the third month against bacterial rot disease. Therefore, the results showed that biosynthesized Ag NPs were effective in controlling pathogenic bacteria and can replace agricultural toxins. Manuscript profile
List of Articles Zeinab Fotoohiyan
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