Isolation and identification of salinity and drought tolerant bacteria from Jiroft agricultural fields

زینب فتوحیان ¹ (عضوهیات علمی)

Submited date : 2023-02-26 accepted date : 2024-01-05

Keywords: identification, Jiroft, drought, Resistant bacteria and Salinity,

Abstract :

One of the new ways to cope with salinity and reduce its harmful effects is to introduce salt-tolerant bacteria. In this research, indigenous bacteria resistant to salinity and drought were isolated and identified. The growth rate of isolates at different concentrations of salinity (5%, 10%, 15%, 20%, 25%, 35% and 40%), drought(0.50, -0.15, -0.03, -0.49 and -0.73 MPa), pH and 50°C. Then, the production of Auxin, Siderophore, hydrogen cyanide and phosphate solubility were investigated. A total of 42 soil samples were collected, 50 bacteria were obtained. The K4, K10, K12, K14, K15 codes for the Konar-sandal, C18, C10, C11 codes for Karim-abad, A2, A3, A4 codes for Anbar-abad were resistant to salinity. Also, the K4, K14, K15, C8 codes had the ability to grow in water potential at -0.73. The C8 and K4 isolates had the ability to grow at high pH and 50°C. Also, isolate C8 produced the highest amount of Auxin (1.8 μg/ml) and it was the only isolate that dissolved phosphate. The isolates C8 and K14 had the ability to produce Siderophore. Therefore, C8 isolate was Bacillus subtilis as the superior isolate.