Effect of Seasons, Gender and Agrobacterium rhizogenes Strains on Adventitious Root Induction of Male and Female Juniperus communis L.
محورهای موضوعی : مجله گیاهان زینتیMostafa Khoshhal Sarmast 1 , Rudabeh Kordkatoli 2 , Zahra Rezaei 3 , Azim Ghasemnezhad 4
1 - Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
2 - Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
3 - Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
4 - Department of Horticultural Science, Faculty of Plant Production, Gorgan University, Faculty of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
کلید واژه: Season, Adventitious rooting, Common juniper, Dioecy, <i>Agrobacterium rhizogenes</i>,
چکیده مقاله :
Scrutinizing of different aspects in vegetative propagation of Iranian J. communis L. is of prime importance in order to prevent extinction of such valuable conifer. In this research, the effects of different seasons, gender, different chemical compounds (IBA, PBZ, putrescine and sodium nitroprusside) and three strains of Agrobacterium rhizogenes on ex-vitro rooting capacity of male and female J. communis L. were evaluated. Rooting of semi-hard wood J. communis L.cuttings in autumn were notably successful either in female or male plants. Research results indicated that cutting gained in September provides the highest rooting percentage in female plants while no significant differences in rooting ability were found in male plants which were collected in December and September. There were a female-biased root induction trends between genders. Treatment of female cuttings with IBA declined rooting to the half, even in male cuttings it completely suppress root induction ability compared to control plants. Our empirical evidence on presence of genetic difference in random amplified loci of male and female plants to some extent can explain the difference in rooting competency which can be influenced by difference in genetic make up. A4 strain of A. rhizogenes managed to yield 53.5% rooting compared to the other strains in male plants. The gender-mediated adventitious root formation of J. communis L. which has supported with some genetic evidence will pave the way for further fundamental studies to delineate the missing molecular key of rooting mechanisms.
موشکافی جنبههای مختلف افزایش رویشی سرو کوهی ایرانی یکی از مهمترین اولویتها در رابطه با جلوگیری از انقراض این گونهها به حساب میآید. در این پژوهش اثر سویههای مختلف اگروباکتریوم رایزوژنز، ترکیبات هومونی مختلف (ایندول بوتریک اسید، پاکلوبوترازول، پوتریسین و سدیم نیتروپروساید)، فصول مختلف و جنسیتهای متفاوت در توانایی ریشهزایی دو پایه نر و ماده سرو کوهی مورد بررسی قرار گرفت. ریشهزایی قلمههای نیمه خشبی سرو کوهی در هر دو پایه در پاییز موفقیتآمیزتر بود. قلمههای گرفته شده در سپتامبر بالاترین درصد ریشهزایی را در پایه ماده داشتند در حالی که تفاوت معنیداری در توانایی ریشهزایی در پایههای نر که در دسامبر و سپتامبر گرفته شده بود دیده نشد.گرایش به ریشهزایی در پایه ماده بیشتر از نر بود. تیمار پایههای ماده با IBA ریشهزایی را به نصف کاهش داد و حتی این تنظیم کننده رشد ریشهزایی را در پایه نر نسبت به کنترل به صفر رساند. تفاوت در درصد ریشهزایی بین پایهها تا حدی بهوسیله تفاوت ژنتیکی مشاهده شده در دو پایه با استفاده از تکثیر تصادفی مکانهای ژنی آنها قابل توجیه خواهد بود. سویه A4 اگرو باکتریوم رایزوژنز در مقایسه با دیگر سویهها ریشهزایی را در پایه ماده به 5/53 درصد رساند. تشکیل ریشههای نابجا مرتبط با جنسیت در سرو کوهی که به کمک دادههای ژنتیکی حمایت میشود راه را برای مطالعات پایهای برای آشکار نمودن حلقه گم شده در سازوکار ریشهزایی گیاهان آشکار خواهد نمود.
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