شناسایی اینتگرون، کاست های ژنی و مقاومت چنددارویی در سویه های اشریشیاکلی یوروپاتوژنیک جدا شده از نمونه های کلینیکی شهرستان بوشهر
محورهای موضوعی : میکروب شناسی مولکولی
کلید واژه: عفونت دستگاه ادراری, اشریشیا کلی یوروپاتوژنیک, مقاومت چند دارویی (MDR), اینتگرون‌ها, کاست‌های ژنی. ,
چکیده مقاله :
سابقه و هدف: اینتگرون ها و کاست های ژنی یکی از مکانیسم های اصلی گسترش مقاومت چندگانه در سویه های اشریشیا کلی می باشند. هدف از این پژوهش، ارزیابی شیوع اینتگرون‌های کلاس 1 تا 3 و کاست های ژنی و تعیین ارتباط آن در ایجاد مقاومت چنددارویی در جدایه های اشریشیا کلی یوروپاتوژنیک جدا شده از عفونت‌های دستگاه ادراری در شهرستان بوشهر بود. مواد و روش ها: این پژوهش به صورت مقطعی-توصیفی بر روی140 سویه اشریشیاکلی جداشده از نمونه‌های عفونت ادراری در شهرستان بوشهر انجام شد. با استفاده از کشت در محیط های اختصاصی و آزمون های استاندارد بیوشیمیایی، تعیین هویت سویه های اشریشیا کلی انجام شد. حساسیت آنتی بیوتیکی جدایه ها نسبت به 18 آنتی بیوتیک ارزیابی گردید. در نهایت با استفاده از پرایمرهای اختصاصی فراوانی اینتگرون‌های کلاس 1تا 3 در جدایه‌ها با روش Single PCR تعیین گردید. یافته ها: از مجموع نمونه های مورد پژوهش بیشترین میزان مقاومت آنتی‌بیوتیکی در جدایه های اشریشیاکلی مربوط به آنتی‌بیوتیک‌های آموکسی‌سیلین و آمپی‌سیلین به ترتیب با فراوانی (82% ) و (80% ) مشاهده شد. میزان فراوانی اینتگرون های کلاس 1و2 به ترتیب تعداد (29)72 /20 % و(10) 7/11 % بود اما اینتگرون کلاس 3 در هیچکدام از جدایه‌ها شناسایی نشد. میزان فراوانی کاست های ژنی مربوط به اینتگرون کلاس 1، تعداد(25) 17/92% و برای اینتگرون کلاس2، (8) 5/67% بود. نتیجه گیری: میزان فراوانی اینتگرون کلاس 1 و کاست های ژنی مربوط به آن ها در سویه‌های اشریشیاکلی مورد مطالعه نسبت به سایر اینتگرون ها بودند. از این رو احتمالا می تواند در گسترش سویه‌هایی با مقاومت چند دارویی نقش داشته باشد.
Background & Objectives: Integrons and gene cassettes are one of the main mechanisms of the development of multi-drug resistant Escherichia coli strains.The aim of this research was to evaluate the prevalence of class 1 to 3 integrons and determine its relationship increating multi-drug resistant Escherichia coli uropathogenic isolated from urinary tract infection in Bushehr. Material and Methods: This descriptive cross-sectional study was performed on 140 E.coli isolated from samples of urinary tract infections in the city of Bushehr. Then by using specific culture media and standard biochemical tests, identification of strains of E.coli was done. Antibiotic sensitivity of isolates to 18 antibiotics was tested. Finally, to determine whether the E.coli isolates carried integrons, the conserved regions were amplified with the degenerate primer and Single PCR method was used to determine frequency of class 1 to 3 of integrons. Results: Among the 140 isolates tested, the highest antibiotic resistance was related to amoxicillin and ampicillin with the frequency of 82% and 80%, respectively. The prevalence of class 1 and 2 integrons was (29) 20.72% and (10) 7.11%, respectively while class 3 integron was not detected in any of the isolates. The frequency of gene cassettes in integron class 1 was (25) 17.92% and for class 2 integron was (8) 5.67%. Conclusion: The prevalence of class 1 integron and related gene cassettes among Escherichia coli uropathogenic strains higher than other integrons, and this factor can probably important role in the spread of multidrug resistance strains.
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