Detection of integrons and gene cassettes among multidrug resistant (MDR) Escherichia coli uropathogenic strains isolated from clinical specimens in Bushehr
Subject Areas : Molecular Microbiology
Keywords: Urinary tract infection (UTI), Uropathogenic Escherichia coli (UPEC), Multidrug resistant (MDR), Integrons, Gene Cassette. ,
Abstract :
Background & Objectives: Integrons and gene cassettes are one of the main mechanisms of the development of multi-drug resistant Escherichia coli strains.The aim of this research was to evaluate the prevalence of class 1 to 3 integrons and determine its relationship increating multi-drug resistant Escherichia coli uropathogenic isolated from urinary tract infection in Bushehr. Material and Methods: This descriptive cross-sectional study was performed on 140 E.coli isolated from samples of urinary tract infections in the city of Bushehr. Then by using specific culture media and standard biochemical tests, identification of strains of E.coli was done. Antibiotic sensitivity of isolates to 18 antibiotics was tested. Finally, to determine whether the E.coli isolates carried integrons, the conserved regions were amplified with the degenerate primer and Single PCR method was used to determine frequency of class 1 to 3 of integrons. Results: Among the 140 isolates tested, the highest antibiotic resistance was related to amoxicillin and ampicillin with the frequency of 82% and 80%, respectively. The prevalence of class 1 and 2 integrons was (29) 20.72% and (10) 7.11%, respectively while class 3 integron was not detected in any of the isolates. The frequency of gene cassettes in integron class 1 was (25) 17.92% and for class 2 integron was (8) 5.67%. Conclusion: The prevalence of class 1 integron and related gene cassettes among Escherichia coli uropathogenic strains higher than other integrons, and this factor can probably important role in the spread of multidrug resistance strains.
Mokracka J , Oazynska A , Kanznowski A. Increased frequency of integrons and B-lactamase-coding genes among exteraintestinal Escherichia coli isolated with a 7-Year interval, Antonie Van Leeuwenhoek, 2013;103:163-174.
Asadi S, Solhjoo K, Kargar M, Rezaeian AA. Phylogenic groups of Escherichia coli strains isolated from urinary tract infection in Jahrom city, southern Iran. Journal of Microbial World.2011; 3 (4): 240-245
Al-Yasi, AAA, Al Saadi kA. Molecular detection of genes responsible for multidrug resistance in uropathogenic Esherichia coli, Iranian journal of ichthyology 9(icab, special issue 2022: 69-78.
Gümüs D , Kalayci Yükse F , Camadan FD , Oral M ,Macunluoğlu AC , Ang küçüker M. High prevalence of class 1 and class 2 integrons in uropathogenic E. coli strains (UPECs) and their relationship with antibiotic resistance, phylogeny and virulence. J Ist Faculty Med 2022; 85(1):77-85.
Sarra S, Arsene MMJ, Grigorievna V E Victorovna PI, Vyacheslavovna YN, Nikolaïevna,BM. Antibiotic resistance pattern of uropathogenic Escherichia coli isolated from children with symptomatic urinary tract infection in Moscow, int. J. One health, 2021; 7(2):212-219.
Shokri D , Mobasherizadeh S , Fatemi SM , Moayednia R , Sadeghi Naeini M. Hospital based surveillance of carbapenem resistance in multidrug-resistant(MDR) strains of Enterobacter and Escherichia coli in Isfahan. Journal of Microbial World 2015; 8(1):64-75
Lina TT, Rahman SR, Gomes DJ. Multiple-Antibiotic Resistance Mediated by Plasmids and Integrons in Uropathogenic Escherichia coli and Klebsiella Pneumoniae, Bangladesh J Microbiol, 2007 ;(24), 1, 19-23.
Domingues S, Silva G,Nielsin K. Vehicle and pathways for horizontal dissemination in bacteria, Mob Gene El, 2012; 2, 5, 211-223.
Mazel D. Integrons:agents ofbacterialevolution, Nat Rev Microbiol, 2006;4(8):608-20
Ochoa SA, Cruz-Cordova A, Luna-Pineda VM, Reyes-Grajeda JP, Cazares-Dominguesz V, et al. Multidrug And Extensively Drug-Resistant Uropathogenic Escherichia Coli Clinical Strains: Phylogenic Groups Widely Associated with Integrons Maintain High Genetic Diversity, ORGINAL RESEARCH fmicb.2016; 02042.
Shi L,Fujihara K,Sato T ,Ito H, et al. Distribution and characterization of integrons in various serogroups of Vibrio cholera strains isolated from diarrhoeal patients between 1992 and 2000 in Kolkata, India, J Med Microbiol, 2006;55, 575-583.
Yu H , Lee J, Kang H, Ro D, et al. Changes in Gene Cassettes of Class 1 Integrons among Escherichia coli Isolates from Urine Specimens Collected in Korea during the Last Two Decades, J clin Microbiol, 2003; 41,12 ,5429–5433
FluitA, Schmitz F. Resistance integrons and super- integrons, CM I,2004; 10, 272-288.
MarquezC,LabbateM,RaymondoC,FernandezJ,GestalA,HollyM,BorthagarayG,StokesH. Urinary Tract Infection in a South American Population :Dynamic Spread of Class 1 Integrons and Multidrug Resistance by Homologous and Sit-Spesific Ricombination , J Clin Microbiol, 2008; P.3417-3425.
Kargar M, Mohammadalipour Z, Doosti A, Lorzadeh S, Japoni- Nejad A. High Prevalence of Class 1 to 3 Integron Among Multidrug- Resistance Diarrheagenic Escherichia coli in Southwest of Iran, Osong Public Health Res Perspect, 2014; 5, 4, 193-198.
Machado E, CantonR, Baquero F, Galan J, et al. Integron Content of Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains over 12 Years in a Single Hospital in Madrid, Spain, Antimicrob Agents Chemother, 2005; 49, 5, 1823-1829.
White P,Mclver C,Rawlinson W. Integrons and Gene Cassettes in the Enterobacteriaceae, Antimicrob. Agents Chemother, 2001; 45(9):2658-2661.
Oliveira-Pinto C, Diamantino C, Oliveira PL, Reis MP, Costa PC, et al. Occurrence and characterization of class 1 integrons in Escherichia coli from healthy individuals and those with urinary infection, Journal of Medical Microbiology 2017;66:577–583.
Partridge S ,Tsafnat G,Coiera E ,Iredell J. Gene cassettes and cassette arrays in mobile resistance integrons, FEMS Microbiol Rev,(2009) ;33 , 757-784.
Falakian Z,Nikokar I, Nafisi,M , Karimi,A,Validi,M . Frequency of Class1 Integrons among Escherichia coli Isolates of Patients with Urinary Tract Infectin,Iran, J Clin Infect Dis, 2011; 6,.4,157-160.
Haddadi A,Ghezeljeh M ,Shavandi M. Prevalence of class 1 and 2 integrons among the multidrug resistant uropathogenic strains of Escherichia coli, Asian Biomed, 2015;9.1,45-49.
Yekani M, Memar MY, Baghi HB, Sefidan FY, Alizadeh N, Ghotaslou R. Association of integrons with multidrug-resistant isolates among phylogenic groups of uropathogenic Escherichia coli, Microbiology Research 2018; 9:7484.
Nojoomi F, Vafaee M, Rajabi Vardanjan Hi. The Relationship Between Class I and II Integrons and Antibiotic Resistance Among Escherichia coli Isolates From Urinary Tract Infections, Int J Enteric Pathog. 2018; May; 6(2):45-47.
KOT B. Antibiotic Resistance among Uropathogenic Escherichia coli Polish Journal of Microbiology 2019; Vol. 68, No 4, 403–415.
Mirnezami M, Ranjbar R, Niakan M., Ahmadi MH. Frequency of Antimicrobial,., Resistance and Class 1 and 2 Integrons in Escherichia Coli Strains Isolated from Urinary Tract Infection, Iranian Journal of Pharmaceutical Research (2022 19 (3): 282-287.(
Li W, Ma J, Sun X, Liu M, Wang H, Antimicrobial Resistance and Molecular Characterization of Gene Cassettes from Class 1 Integrons in Escherichia coli Strains Microbial Drug Resistance, 2022; Volume 28, Number 4.
Farahani A, Dastranj M, Shamseddin J, Veisi H, Soltani S, Kalantar H. Antibiotic resistance and presence of integrin class 1 and class 2 genes amongst Escherichia coli isolates of urine specimens of inpatients and outpatients in Ahvaz, Southern of Iran. Journal of Microbial World 2023; 16 (3):167-177
Rowe- mangnus D,Mazel D, Therole of integrons in antibiotic resistance gane capture, J. Med. Microbiol, 2002; 292, 115-125.
Al-Azawi I H , Al-Bidiri M S , Distribution of Integron III and Phylogenic Clade amon MDR Uropathogenic E.coli from Patient in Al-Diwaniyah City , Iraq . (2022); Wiad Lek, 75(5p2):1254-1260.
Zeighami H,Haghi F,Masumian N.,Hemmati F, Samei A, NaderiG,Distribution of Integrons and Gene Cassettes Among Uropathogenic and Diarrheagenic Escherichia coli Isolates in Iran, M D R, 2015;V00,N00.
Gundogdu A ,Beverley Long Y,Liegh Vollmerhausen T Katouli M. Antimicrobial resistantce and distribution of sul genes and integron-associeted intI among uropathogenic Escherichia coli in Queensland, Australia, J med microbiol, 2011; 60,1633-1642.
Farshad S,Japoni A, Hosseini M . Low Distribution of Integrons among Multidrug Resistan E.coli Strains Isolated From Children with Community-Acquired Urinary Tract Infections in Shiraz, Iran. Pol. J. Microbial, 2008; l.57, .3, 193-198.
Recchia G, Stokes H, Hall R . Characterisaion of slecific and secondary recombination sites recognized by the integron DNA integrase, NAR, 1994; 22, 11, 2071-2078.
EL-Najjar N, Farah M ,Tokajian S, Antibiotic resistance patterns and sequencing of class 1 integron from uropathogenic Escherichia coli in Lebanon, Lett Appl Microbiol, 2010 ;51, 456-46.
Dawes F. Antibiotic resistance genes located in integrons isolated from Escherichia coli recovered from humans and animals, university of Wollongong thesis collection ; 2009.
Al-Assil B,Mahfoud M ,Hamzeh A. First report on class 1 integrons and trimethoprim- resistant genes from dfrA group in uropathogenic E.coli (UPEC) from the aleppo area in Syria, Mob Gene El, 2013; :3: (25204-1-6).