In vitro behavior of silk fibroin-coated calcium magnesium silicate scaffolds

Subject Areas : Finite Element Modeling

Masoud Hafezi ¹ , Hossein Mohammadi ² , Ali Nadernezhad ³ , Pardis Fazlali ⁴ , Noor Azuan Abu Osman ⁵

1 - Nanotechnology and Advanced Materials Division, Materials and Energy Research Center, Iran
2 - School of Materials and Mineral Resources Engineering, Universiti Sains Malaysia, Engineering Campus, 14300 Nibong Tebal, Penang, Malaysia
3 - Biomaterials Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center, Alborz, Iran
4 - Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur
5 - Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur

Received: 2016-02-10 Accepted : 2016-03-12 Published : 2016-03-01

Keywords: coating, In vitro, Scaffold, Calcium magnesium silicate, Silk,

Abstract :

Bioceramic scaffolds such as silicate bioceramics have been widely used for bone tissue engineering. However, their high degradation rate, low mechanical strength and surface instability are main challenges compromising their bioactivity and cytocompatibility which further negatively affect the cell growth and attachment. In this study, we have investigated the effects of silk fibroin coating on the tricalcium magnesium silicate scaffolds in term of biological behavior for bone tissue engineering. The microstructure, morphology, cell adhesion and chemical composition of coated scaffolds were analyzed by scanning electron microscopy and Fourier transform infrared spectroscopy. Also, MTT assay test showed that both coated and uncoated scaffolds supported growth of mouse embryonic fibroblast cell. However, the coated scaffold revealed a higher cell proliferation than uncoated one. All the results postulated that silk fibroin was successfully coated on the scaffold and improved the biological properties of scaffold indicating a promising biomaterial for bone tissue engineering application.

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