Molecular Analysis, Serotyping and Antibiogram Pattern of Salmonella in Marketed Local, Industrial and Breeder Poultry Eggs in Tabriz City, Iran

Subject Areas : food microbiology

S. Danesh Ghohar ¹ , A. Javadi ² , A. R. Dehnad ³

1 - M. Sc. Student of Microbiology, Cellular and Molecular Biology Department, Institute of Rabe –Rashidieh, Tabriz, Iran.
2 - Associate Professor of Food Hygiene, Biotechnology Research Center, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
3 - M. Sc. Graduate of the Department of Biotechnology East Azerbaijan Research and Education Center Agricultural and Natural Resources, Areeo, Tabriz-Iran. Higher education institute of Rabe-Rashidieh, Tabriz, Iran.

Received: 2017-06-15 Accepted : 2017-06-15 Published : 2017-07-01

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Abstract :

Food borne diseases are considered as the major problems for public health in the world and salmonella serotypes are the most important pathogens causing these types of diseases. Present study was carried out for the isolation of salmonella and determination of their antibiotic sensitivity and serovars from local, industrial and breeder poultry eggs. In this cross-sectional survey, 300 samples of local, industrial and breeder poultry eggs (each containing 100 samples) were collected from Tabriz city, Iran and transferred to the laboratory of food hygiene, Islamic Azad University of Tabriz. Isolation of salmonella from egg yolk was performed according to the Iranian National Standards. For definitive detection of salmonella, Biochemical tests, serotyping, and PCR tests were performed followed by Antibiogram test. From a total of 300 analyzed eggs, just 3 out of local samples (3%) were contaminated with salmonella enteritidis. 100 percent of isolates were sensitive to Ciprofloxacin, Gentamycin, Neomycin, Trimethoprim and Sulfamethoxazole. All of the isolates were found to be resistant to at least 5 of 9 evaluated antibiotics. According to the results of this study, the rate of contamination in local eggs supplied and distributed in Tabriz was %3 but the industrial and breeder poultry eggs were recognized safer for public use. According to these findings, it is therefore recommended to pay further attention to cold storage of local eggs.