Preparation and Characterization of Pullulan-Soy Protein Concentrate Biocomposite Film
Subject Areas : food microbiologyF Hedayati Rad 1 , F. Khodaiyan 2 , S. E. Hossaini 3 , A. Sharifan 4
1 - Ph. D. Student of the Department of Food Science and Technology, Science and Research Branch, Islamic
Azad University, Tehran, Iran.
2 - Assistant Professor of the Department of Food Science, Engineering and Technology, Faculty of Agricultural
Engineering and Technology, University of Tehran, Karaj, Iran
3 - Associate Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic
Azad University, Tehran, Iran.
4 - Associate Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic
Azad University, Tehran, Iran.
Keywords: Functional Properties, Pullulan, Soy Protein Concentrate,
Abstract :
The non-biodegradable nature of plastic packaging has brought about an attention in bio-basedpackaging materials. Edible composite films were prepared using soy protein concentrate (SPC) and pullulan(PUL) biopolymer at five different ratio (100:0; 70:30; 50:50; 30:70 and 0:100) with glycerol as plasticizer bycasting-evaporation method. The thickness, mechanical properties, water vapor permeability (WVP), solubilityand color of the film specimens were investigated. Increasing PUL content decreased the thickness, and tensilestrength but the visual properties, elongation at break, WVP, and solubility in water of the films improved,simultaneously. In addition, the crystalline properties and microstructure of the bio-composites werecharacterized by X-ray diffraction and Scanning Electron Microscopy (SEM), respectively. Homogenousmicrostructure and crystalline properties of the films showed good affinity with mechanical and functionalproperties of the films. The optimum mechanical properties and WVP were obtained at the SPC to PUL ratio of50:50. These findings support the potential of the equal proportion of SPC/PUL biocomposite as a foodpackaging material.
Anon. (2016). Food and Agriculture
Organization. FAOSTAT. http://faostat3.fao.org/
browse/Q/QC/E Accessed Augest 2016.
Emiroğlu, Z. K., Yemiş, G. P., Coşkun, B. K.
& Candoğan, K. (2010). Antimicrobial activity
of soy edible films incorporated with thyme and
oregano essential oils on fresh ground beef
patties. Meat Science. 86(2), 283-288.
doi:10.1016/j.meatsci.2010.04.016
Friesen, K., Chang, C. & Nickerson, M.
(2015). Incorporation of phenolic compounds,
rutin and epicatechin, into soy protein isolate
films: Mechanical, barrier and cross-linking
properties. Food chemistry. 172, 18-23.
Galus, S., Mathieu, H., Lenart, A. &
Debeaufort, F. (2012). Effect of modified starch
or maltodextrin incorporation on the barrier and
mechanical properties, moisture sensitivity and
appearance of soy protein isolate-based edible
films. Innovative food science and emerging
technologies. 16, 148-154.
González, A. & Igarzabal, C. I. A. (2013).
Soy protein–Poly (lactic acid) bilayer films as
biodegradable material for active food
packaging. Food Hydrocolloids. 33(2), 289-296.
González, A. & Igarzabal, C. I. A. (2015).
Nanocrystal-reinforced soy protein films and
their application as active packaging. Food
Hydrocolloids. 43, 777-784.
Gounga, M. E., Xu, S. Y. & Wang, Z. (2007).
Whey protein isolate-based edible films as
affected by protein concentration, glycerol ratio
and pullulan addition in film formation. Journal
of Food Engineering. 83(4), 521-530.
Hassannia-Kolaee, M., Khodaiyan, F.,
Pourahmad, R. & Shahabi-Ghahfarrokhi, I.
(2016). Development of ecofriendly
bionanocomposite: Whey protein
isolate/pullulan films with nano-SiO 2.
International journal of biological
macromolecules. 86, 139-144.
Hassannia-Kolaee, M., Khodaiyan, F. &
Shahabi-Ghahfarrokhi, I. (2016). Modification
of functional properties of pullulan–whey
protein bionanocomposite films with nanoclay.
Journal of food science and technology. 53(2),
1294-1302.
Jensen, A., Lim, L. T., Barbut, S. & Marcone,
M. (2015). Development and characterization of
soy protein films incorporated with cellulose
fibers using a hot surface casting technique.
LWT-Food Science and Technology. 60(1), 162-
170.
Kristo, E., Biliaderis, C. & Zampraka, A.
(2007). Water vapour barrier and tensile
properties of composite caseinate-pullulan films:
Biopolymer composition effects and impact of
beeswax lamination. Food Chemistry. 101(2),
753-764.
Kurose, T., Urman, K., Otaigbe, J. U.,
Lochhead, R. Y. & Thames, S. F. (2007). Effect
of uniaxial drawing of soy protein isolate
biopolymer film on structure and mechanical
properties. Polymer Engineering and Science.
47(4), 374-380.
Leathers, T. (2003). Biotechnological
production and applications of pullulan. Applied
microbiology and biotechnology. 62(5), 468-
473.
Motedayen, A. A., Khodaiyan, F. & Salehi,
E. A. (2013). Development and characterisation
of composite films made of kefiran and starch.
Food Chemistry. 136(3), 1231-1238.
Shahabi-Ghahfarrokhi, I., Khodaiyan, F.,
Mousavi, M. & Yousefi, H. (2015). Effect of γ-
irradiation on the physical and mechanical
properties of kefiran biopolymer film.
International journal of biological
macromolecules. 74, 343-350.
Siracusa, V., Rocculi, P., Romani, S. & Rosa,
M. D. (2008). Biodegradable polymers for food
packaging: a review. Trends in Food Science
and Technology. 19(12), 634-643.
Stankovic, I. (2011). Pullulan Chemical and
Technical Assessment (CTA). Paper presented at
the Chemical and Technical Assessment 65th
JECFA.
Su, J. F., Huang, Z., Yuan, X. Y., Wang, X.
Y. & Li, M. (2010). Structure and properties of
carboxymethyl cellulose/soy protein isolate
blend edible films crosslinked by Maillard
reactions. Carbohydrate Polymers. 79(1), 145-
153.
Su, J. F., Yuan, X. Y., Huang, Z., Wang, X.
Y., Lu, X. Z., Zhang, L. D. & Wang, S. B.
(2012). Physicochemical properties of soy
protein isolate/carboxymethyl cellulose blend
films crosslinked by Maillard reactions: Color,
transparency and heat-sealing ability. Materials
Science and Engineering: C. 32(1), 40-46.
Tong, Q., Xiao, Q. & Lim, L. T. (2008).
Preparation and properties of pullulan–alginate–
carboxymethylcellulose blend films. Food
Research International. 41(10), 1007-1014.
Trinetta, V., Cutter, C. N. & Floros, J. D.
(2011). Effects of ingredient composition on
optical and mechanical properties of pullulan
film for food-packaging applications. LWT -
Food Science and Technology. 44(10), 2296-
2301.
Wihodo, M. & Moraru, C. I. (2013). Physical
and chemical methods used to enhance the
structure and mechanical properties of protein
films: A review. Journal of Food Engineering.
114(3), 292-302.
Wu, J., Zhong, F., Li, Y., Shoemaker, C. &
Xia, W. (2013). Preparation and characterization
of pullulan–chitosan and pullulan–
carboxymethyl chitosan blended films. Food
Hydrocolloids. 30(1), 82-91.
Zhang, B., Wang, D. F., Li, H. Y., Xu, Y. &
Zhang, L. (2009). Preparation and properties of
chitosan–soybean trypsin inhibitor blend film
with anti-Aspergillus flavus activity. Industrial
Crops and Products. 29(2), 541-548.
Zolfi, M., Khodaiyan, F., Mousavi, M. &
Hashemi, M. (2014). Development and
characterization of the kefiran-whey protein
isolate-TiO 2 nanocomposite films. International
journal of biological macromolecules. 65, 340-
345.