بررسی اثر محلول‌پاشی سیلیس و تنش شوری بر برخی صفات فیزیولوژیکی گیاه روغنی کاملینا (Camelina sativa)

محورهای موضوعی : تنش

ابراهیم فانی ¹ (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، بهبهان، ایران)
شکوفه حاجی هاشمی ² (گروه زیست شناسی، دانشکده علوم پایه، دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان، بهبهان، ایران)

تاریخ دریافت : 1400/12/13 تاریخ پذیرش : 1401/02/24 تاریخ انتشار : 1402/01/01

کلید واژه: کلروفیل a, کلروفیل b, ظرفیت آنتی‌اکسیدانی, قند محلول, پروتئین محلول,

چکیده مقاله :

به منظور بررسی اثرات تنش شوری و محلول پاشی سیلیس بر روی گیاه روغنی کاملینا، مطالعه ای در اتاقک رشد آزمایشگاه گروه زیست شناسی دانشگاه صنعتی خاتم الانبیاء (ص) بهبهان در 3 تکرار با طرح آزمایشی فاکتوریل در قالب پایه کاملاً تصادفی به اجرا درآمد. تیمارها شامل تنش شوری در دو سطح (بدون تنش و تنش 100 میلی مولار) و محلول سیلیکات پتاسیم در دو سطح ( عدم محلول پاشی و محلول پاشی 5 میلی‌مولار) بود. هدف از این مطالعه، بررسی اثرات تیمار خارجی سیلیس بر صفات فیزیولوژیکی و بیوشیمیایی گیاه کاملینا در شرایط تنش شوری و ارزیابی نقش مواد اسمزی مانند قند محلول و پروتئین محلول در کاهش اثرات مضر تنش شوری در گیاه کاملینا به عنوان یک گیاه مهم روغنی بود. نتایج نشان داد که تنش شوری در سطح معنی داری سبب کاهش میزان کلروفیل‌های a، b و کل در گیاه کاملینا شد، درحالیکه تیمار سیلیس در سطح معنی‌داری سبب کاهش اثرات مضر شوری برآنها شد. میزان قند محلول برگ در پاسخ به تنش شوری 46 درصد کمتر از گیاه شاهد بود. تنش شوری توام با سیلیس میزان قند محلول را 27 درصد بیشتر از گیاه شاهد افزایش داد. همچنین نتایج نشان داد که در تیمار سیلیس توام با 100 میلی‌مولار کلریدسدیم میزان پروتئین‌های برگ تقریبا 10 درصد کمتر از گیاه شاهد بود. نتایج اندازه گیری ظرفیت آنتی‌اکسیدانی FRAP گیاه نشان داد که در پاسخ به تیمار سیلیس بدون شوری تفاوت معنی داری در میزان ظرفیت آنتی‌اکسیدانی مشاهده نشد، در حالی که در تنش شوری توام با تیمار سیلیس سبب کاهش 9 درصد کمتر از گیاه شاهد شد. نتایج این تحقیق نشانگر نقش مفید سیلیس به عنوان یک ترکیب سازگار با محیط زیست به منظور افزایش مقاومت گیاهان به تنش شوری بود.

چکیده انگلیسی:

In order to investigate the effects of salinity stress and foliar application of silica on Camelina sativa oil plant, a factorial study was carried out in the growth chamber of the laboratory of the Department of Biology, Behbahan Khatam Alanbia University of Technology, in 3 replications based on a completely randomized block design. Treatments included salinity stress at two levels (0 and 100 mM NaCl) and potassium silicate solution at two levels (0 and 5 mM). The physiological and biochemical traits of Camelina sativa under salinity stress were evaluated along with the role of osmotic substances such as soluble sugar and soluble protein in reducing the harmful effects of salinity stress. Results showed that salinity stress significantly reduced the amount of chlorophyll a, b, and total in Camelina sativa, while silica treatment significantly reduced the harmful effects of salinity. Soluble sugar content of leaves in response to salinity stress was 46% lower than in the control plant. Salinity stress combined with silica increased the amount of soluble sugar 27% more than the control plant. Results also showed that in the treatment with silica under 100 mM salinity level, the amount of leaf proteins was almost 10% less than the control plants. The results of measuring the plant’s antioxidant capacity of FRAP showed that in response to silica treatment without salinity, no significant difference was observed in the amount of antioxidant capacity of FRAP, while in the combined treatment of salinity stress + silica it decreased by 9% compared with the control plants. The results of this study supports the beneficial role of silica as an environmentally friendly compound to increase plant resistance to salinity stress.

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