ارزیابی اثرات محلول پاشی اسید سالیسیلیک و نانو کلات آهن بر برخی خصوصیات بیوشیمیایی و مورفوفیزیولوژیکی بنفشه معطر (odorata L. Viola)

محورهای موضوعی : ژنتیک

اسماء حسنوند ¹ , لیلا فهمیده ² , فاطمه بیدرنامنی ³

1 - گروه گیاهان دارویی، ادویه‌ای و نوشابه‌ای دانشگاه زابل، زابل، ایران
2 - گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
3 - گروه زراعت و اصلاح نباتات، پژوهشکده کشاورزی، پژوهشگاه زابل، زابل، ایران

تاریخ دریافت : 1399/04/24 تاریخ پذیرش : 1399/07/22 تاریخ انتشار : 1401/04/01

کلید واژه: کربوهیدرات, موسیلاژ, رنگیزه‌های فتوسنتزی, القا کننده, بنفشه معطر,

چکیده مقاله :

به منظور ارزیابی تأثیر غلظت‌های مختلف اسید سالیسیلیک و نانو کلات آهن بر میزان موسیلاژ، کربوهیدرات ها و برخی صفات مورفوفیزیولوژیکی گیاه بنفشه معطر، آزمایشی به صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار انجام شد. پس از کشت بذرها در گلدان، تیمارهای آزمایشی شامل محلول پاشی اسید سالسیلیک (در سه سطح صفر (شاهد)، 75/0 و 5/1 میلی‌گرم در لیتر) و نانو کلات آهن (در دو سطح صفر (شاهد) و 1 گرم در لیتر) در مرحله گیاهچه ای اعمال شد. نتایج حاصله نشان داد که بیشترین مقادیر کاروتنوئیدها، کلروفیل a، b و کل، پروتئین و فعالیت کاتالاز در گیاهان محلول پاشی شده با 5/1 میلی‌گرم در لیتر اسید سالسیلیک حاصل شد و همچنین بیشترین مقدار عناصر معدنی سدیم، مس، موسیلاژ و وزن‌تر بوته هم در تیمار محلول پاشی یک گرم در لیتر نانو کلات وجود داشت. سایر نتایج نیز مشخص ساخت که بیشترین مقدار کربوهیدرات در محلول پاشی تیمار 5/1 میلی‌گرم در لیتر اسید سالسیلیک و یک گرم در لیتر نانو کلات آهن بدست آمد. در این مطالعه افزایش 12 و 19 درصدی ارتفاع در بوته های محلول پاشی با اسید سالسیلیک (5/1 میلی‌گرم در لیتر) و نانو کلات آهن (1 گرم در لیتر) نسبت به شاهد مشاهده شد. بر اساس نتایج حاصله می توان تیمار اسید سالسیلیک و نانو کلات آهن را به منظور افزایش خصوصیات رشدی و داروئی این گیاه پیشنهاد نمود.

چکیده انگلیسی:

 To evaluate the effects of different concentrations of salicylic acid and nano iron chelate on the mucilage and carbohydrate values as well as some morpho-physiological traits of Viola aodorata L. species, a factorial experiment was defined based on randomized complete block design with three replications. After planting the seeds in pots, experimental treatments included foliar application of salicylic acid at three levels (0, 0.75 and 1.5 mg / L) as well as nano iron chelate at two levels (0 and 1 g / L) was applied at seedling stage. According to the results, the highest amounts of carotenoid, chlorophyll a, chlorophyll b as well as the total chlorophyll, protein and catalase were observed in specimen treated with 1.5 mg / L salicylic acid. Moreover, the highest amounts of sodium, copper, mucilage and fresh plant weight were detected in specimen treated with 1g / L iron nano chelate. The results also demonstrated that the highest amount of carbohydrate was found  in specimen treated with 1.5 mg / L salicylic acid and 1 g / L nano-iron chelate. In this investigation, the foliar application of salicylic acid (1.5 mg / L) and iron nano chelate (1 g / L) led to a 12 and 19 percent increase in the plant height compared to the untreated sample, respectively. Therefore, treatment with salicylic acid and nano-iron chelate could be recommended to increase the growth and medicinal properties of this plant species.

منابع و مأخذ:

Agarawal, S., Sairam, R.K., Srivasta, G.C. and Meena, R. C. (2005). Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes. Biological Plant. 49(4): 541-550.

Aghazadehkhalkhali, D., Mehrafarin, A., Abdosi, V. and Naghdibadi, H. A. (2014). The operation of fleawort seed and mucus in response to nano-fertilizer spraying of iron and potassium kalat. Journal of Herbs. 14(4): 23-34.

Akbarian M.M., Heidari Sharifabad, H., Noormohammadi, G. and Darvish Kojouri, F. (2012). The effect of potassium, zinc and iron foliar application on the production of saffron (Crocus sativa). Annals of Biological Research. 3 (12): 5651 - 58.

Amaliotis, D., Velemis, D., Bladenopoulou, S. and Karapetsas, N. (2002). Leaf nutrient levels of strawberries (cv. Tudla) in relation to crop yield. Acta Horticulturae. 567: 447 - 50.

Amiri, A., Siroosmehr, A. R., Yadolahi, P., Asgharipoor, M. R. and Esmailzadehbahabadi, S. (2016). The effect of drought and spraying of salicylic acid and chitosan on photosynthetic pigments and antioxidant enzymes of safflower, Agricultural Crop. 18(2): 453-466.

Arnon, A. N. (1976). Method of extraction of chlorophyll in the plants. Journal Agronomy, 112: 23-121.

Arora, D. S. and Kaur, G. J. (2007). Antibacterial activity of some Indian medicinal plants. Journal of Nationality Medicinal. 61(3): 313-17.

Arvin, M.J., Beidshki, A., Kramt, B. and Maghsodi, K. (2011). The study salicylic acid (SA) role in contrast with drought stress by affecting on morphological and physiological parameters in garlic plant. In: Proceeding of 7th Iranian Horticultural Science Congress, Isfahan Industrial University, 4-7 September.

Asadi Karam, E., Asrar, Z. and Keramat, B. (2016). Effect of methyl jasmonate on proline content and absorption Cu, Fe, Zn and Mg in Lepidium sativum L. subjected to copper toxicity. Journal of Plant Biology.29(2): 243-253.

Askari, M., Amirjani, M. R. and Saberi, T. (2014). Evaluation of the effects of iron nanofertilizer on leaf growth, antioxidants and carbohydrate contents of Catharanthus roseus. Journal of Plant Process and Function Iranin Society of Plant Physiology. 3 (7): 43-56.

Azad, H., Fakheri, B., Mahdinezhad, N. and Parmoon, Q. (2017). The effect of foliar application of nano-iron chelated on antioxidant enzymes activity and flower yield of chamomile genotypes under drought stress condition. Journal of Crop Improvement. 19(2): 257-271.

 Azadbakht, M. (1999). Classification of herbs, Vol.1, Teymoorzade’s Publication, 420 pp.

Baghaie, N. and Maleki Farahani, S. (2014). Comparison of nano and micro chelated iron fertilizers on quantitative yield and assimilates allocation of saffron (Crocus sativus L.). Journal of Saffron Research. 1(2): 156-169.

Bahrani, A.M. and Pourreza, A. (2014). Effects of micronutrients on seed yield and oil content of Brassica napus L. CV Talaeh. Botany. 43(2): 231-233.

Barghi, A., Gholipoori, A., Tobeh, A., Jahan Bakhsh, S., and Jamaati, S. (2014). Survey on the effects of iron nano oxide foliar application on mineral nutrients uptake in potato tuber. Journal of Plant Ecophysiology, 6(16), 1-12.

Bayat, H., Alirezaie, M. and Neamati, H. (2012). Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L.) under salinity stress. Journal Stress Physiology Biochemistry. 8: 258-267.

Beers, G.R. and Sizer, I. V. (1952). A spectrophotometric method for measuring the break down of hydrogen peroxide by catalase. Journal of Biological Chemistry. 195: 133-140.

Bradford, M. M. (1976). A rapiand sensitive method for quantitation of microgram quantitiesof protein utilizing the principle of protein-dye binding. Journal of Analytical Biochemistry. 72: 248- 254.

Dahmardeh, M., Taji, R., Khammari, I. and Hadad, T. (2018). Evaluation of the green synthesis iron and silver nanoparticles of green tea (Camellia sinesis L.) extract on morphological and physiological traits of borage (Borago officinalis L.). Iranian Journal of Medicinal and Aromatic Plants. 34(2): 330-345.

Davoudi, S., Biabani, A., rahemi-karizaki, A., modares sanavi, S., Gholamalipor Alamdari, E., and Zarei, M. (2020). Effects of nano and common forms of iron and zinc chelates on some characteristics of medicinal plant black cumin (Nigella sativa L.). Iranian Journal of Medicinal and Aromatic Plants Research, 36(3), 448-465.

El-Tayeb, M. A. (2005). Response of barley grain to the interactive effect of salinity and salicylic
acid. Plant Growth Regulation. 42: 215-224.

Enteshar, Sh. and Esnaashari, E. (2018). Effects of iron chloride, iron chelate and nano-iron on enzymatic and non-enzymatic antioxidant mechanisms in Melissa officinalis under aluminum treatment. Journal of Plant Process and Function. 7(23): 193-204.

Eraslan, F., Inal, A., Pilbeam, DJ. and Gunes, A. (2008). Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. CV. Matador) grown under boron toxicity and salinity. Plant Growth Regulation. 55: 207-219.

Farsari, S., Moghaddam, M. and Mehdizadeh, L. (2022). Effects of salicylic acid on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress. Journal of Plant Environmental Physiology. 17(65): 57-70.

Fathi-Amirkhiz, K., Amini-Dehagi, M. and Heshmati, S. (2016). The effect of chelated iron on chlorophyll content, quantum efficiency of photosystem II and some biochemical traits of safflower in low irrigation condition. Iran. Journal Field Crops Science. 46 (1): 137 - 45.

Fazeli, A., Zarei, B. and Tahmasebi, Z. (2018). The effect of salinity stress and salicylic acid on some physiological and biochemical traits of Black cumin (Nigella sativa L.). Iranian Journal of Plant Biology. 9(4): 69-83.

Gardner, F.P., Piers, R. and Michelle, L. (2011). Physiology of crop plants. Translation: Koocheki A, and Sarmadnia Gh. 16th ed. Mashhad SID Press. 400 pages.

Ghai, N., Setia, R. C. and Setia, N. (2002). Effect of paclobutrazol and salicylic acid on chlorophyll content, hill activity and yield components in Brassica napus L. Phytomorphol. 52: 83-87.

Ghani, U. K., Saeed, A. and Alam, M. T. (1997). Industryunic Medicine. Department of Pharmacognosy, University of Karachi, India, pp, 310-311.

Ghasemian, V., Shafaghkalvanegh, J. and Pirzad, A. (2017). The effect of fertilizer treatments and irrigation regimes on operation and combination of mucus citron. Journal of Agricultural Kknowledge and Sustainable Production. 27(3): 17-31.

Gheyrati arani, L., Sheykhi, M. and Sharifi T. (2014). Effect of micronutrient application at different growth stages on growth and yield of common bean. Agroecology Journal. 9 (3): 35-47.

Graham, J.G. and Quinn, M.L. (2000). Plants used against cancer – an extension of the work of Jonathan Hartwell. Journal of Ethnopharmacology. 73: 347–377.

Hameri, E., Khadem Al-Rasul, A., Abdollahi, L. and Findout, U. (2005). Study of different levels of cocoa and cake filters on iron, manganese and organic matter of calcareous soils under sugarcane cultivation. Abstract of articles of the 9th Iranian soil science congress, 3-7 September 2005, Tehran, Iran. Soil and Water Conservation Research Center of Iran, Iran. Pp. 88-59.

Hashemi Fadaki, S. E., Fakheri, B. A., Mahdinezhad, N. and Mohammadpour Vashvaei, R. (2017). Effects of nano and nano bio-fertilizer on physiological, biochemical characteristics and yield of roselle (Hibisicus sabdariffa L.) under drought stress. Crops Improvement (Journal of Agricultural Crops Production). 20(1): 45-66.

Hodálová, I., Mereďa Jr, P., Mártonfi, P., Mártonfiová, L. and Danihelka, J. (2008). Morphological characters useful for the delimitation of taxa within Viola subsect. Viola (Violaceae): a morphometric study from the West Carpathians. Folia Geobotanica. 43(1): 83-117.

Hoseinzadeh, A., Chaparzadeh, N. and Deilamghan, K. L. D. (2014). The effects of salicylic acid on growth parameters, esmolites and osmotic potential in radish under salinity tension, Journal of Plant Researches (Iranian Journal of Biology). 27(1): 32-40.

Irigoyen, J.J., Emerrich, D. W. and Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa plant. Physiologia Plantarum. 84: 55-60.

Irrigoyen, J. H., Emerich, D.W. and Sanchez Diaz, M. (1992). Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa plant. Journal of Physiologia Pantarum. 84: 55-66.

Jamali, B., Eshghi, S. and Tafazoli, E. (2013). Vegetative growth, yield, fruit quality and fruit and leaf composition of strwberry cv. ‘Pajaro’ as influenced by salicylic acid and nickel sprays. Journal of Plant Nutrition. 36: 1043-1055.

Janovitz-Klapp, A. H., Richard, F. C., Goupy, P. M., and Nicolas, J. J. (1990). Inhibition studies on apple polyphenol oxadase. Journal of Agricultural Food Chemistry. 38: 926–931

Kalayasundram, N. K., Pateb, P. B. and Dalat, K. C. (1982). Nitrogen need of Plantago ovata in reaction to the available nitrogen in soil. Indian Journal of Agricultural Science. 52: 240 -242.

Kamiab, F., and Mohamadi, H. (2019). Evaluation of the Effects of Fe and Cu Nano Chelates on some Morphological and Physiological Characteristics of Narcissus (Psedudonarcissus narcissus Cv. Jonquil). Journal of Horticultural Science, 33(2): 257-272.

Kashefi, B., Ghods, M. and Mogadam, M. (2015). Study of salicylic acid application on some morphological and physiological traits in clary sage under salinity stress. Journal of Crops Management. 17(2):431-440.

Koochaki, A. and Khajehoseini, M. (2012). Novin farming. Vol.1, publication of Mashhad. 704 pp.

Ladan Moghadam, A., Vattani, H., Baghaei, N. and Keshavarz, N. (2012). Effect of different levels of fertilizer nano_iron chelates on growth and yield characteristics of two varieties of Spinach (Spinacia oleracea L.): Varamin 88 and Viroflay. Research Journal of Applied Sciences, Engineering and Technology. 4(12): 4813-4818.

Lim, T. (2014).Viola odorata. In Edible Medicinal and Non Medicinal Plants Springer. 795-807.

Lim, T. K. (2014). Edible Medicinal and Non Medicinal Plants: Volume 8, Flowers, Springer Science, vNew York, London. 1038p.

Mabberley, D. (1987). The Plant Book. Cambridge University Press. Cambridge, 858p.

Mahdavian, K. (2021). The study of the effects of different concentrations of salicylic acid on improving physiological and biochemical properties of pistachio (Pistacia vera L.) var. Akbari seedlings under salinity stress. Journal of Plant Environmental Physiology. 16(62): 139-150.

Mahdi nezhad, N., Jamalpour, H., Fakheri, BA. and Azad, H. (2019). The study of the effects of different concentrations of salicylic acid on improving physiological and biochemical properties of pistachio (Pistacia vera L.) var. Akbari seedlings under salinity stress. Journal of Plant Environmental Physiology. 14(54): 74-89.

Marschner, H. (1995). Mineral Nutrition of HigherPlants. 2ed. New York: Academic. Press. 889pp.

Mehraban, A. (2017). The effects of iron nano-kalat on operation, Operation Components of Lentil, Environmental Physiology of Journal. 12(45): 27-37.

Mengel, K., and Kirby, EA. (1987). Principles of plant nutrition. International Potash, Inst. 320 p. 24.

Mishra, S. and Dubey, R. S. (2006). Heavy metal uptake and detoxification mechanisms in plants. International Journal of Agricultural Research. 1(2): 122-141.

Moghadam, E., Mahmoodi Sourestani, M., Farrokhian Firozi, A., Ramazani, Z. and Eskandari, F. (2016). The effect of foliar application of iron chelate type on morphological traits and essential oil content of holy basil (Ocimum sanctum). Journal of Crop Improvement. 17 (3): 595-606.

Mohamadipoor, R., Sedaghathoor, S., and Mahboub Khomami, A. (2013). Effect of application of iron fertilizers in two methods 'foliar and soil application' on growth characteristics of Spathyphyllum illusion. Iranian J. Hort. Sci. Technol. 3(1):232-240.

Moradi Marjane, E. and Goldani, M. (2011). Evaluation of different salicylic acid levels on some growth characteristics of pot marigold (Calendula officinalis L.) under limited irrigation. Environmental Stresses in Crop Sciences. 4(1): 33-45.

Movahedi, Z. and Moieni, A. (2019). Effects of iron chelate and nano chelate on some physiological and morphological characteristics of chicory (Cichorium intybusL.) in aeroponic system. Iranian Journal of Medicinal and Aromatic Plants. 35(3): 501-511.

Naderi, M. and danesh shareki, A. (2011). Application of Nanotechnology to Optimize the Formation of Chemical Fertilizers. Journal of Nanotechnology. 165 (4): 22-20.

Najafi, S., Heidari, R. and Jamei, R. (2013) Influence of silver nanoparticles and magnetic field on phytochemical, antioxidant activity compounds and physiological factors of Phaseolus vulgaris. Technical Journal of Engineering and Applied Sciences. 3: 2812-2816.

Nasiri Dehsorkhi, A., Ghanbari, A. and Varnaseri Ghandali, V. (2018). Effect of Foliar Application of Chelate Iron in Common and Nanoparticles Forms on Yield and Yield Components of Cumin (Cuminum cyminum L.) under Drought Stress Conditions. Iranian Journal of Field Crops Research. 16(1): 229-241.

Nazeri, M., Khaleji, H., Labafihoseinabadi, M., Shamsabadi, M. and Rasooli, A. S. (2010). checking iron nano-kalat on quantitative and qualitative factors of wheat. Proceedings of second national congress on the application of nanoparticles in agriculture, Hamedan, Iran.

Nezamdoost, S., Farahzadeh, A. R. and Rasoolisedighian, M. H. (2016). The effects spraying salicylic acid on some physiological and biochemical characteristics of grape. Journal of Research on Fruit. 1(1): 15-29.

Noora, R. and Safahani, AR. (2020). Alleviatory activities of salicylic acid and chitosan in burdock plant (Arctium lappa L.) under drought stress. Journal of Plant Environmental Physiology. 14(56): 39-56.

Nourafcan, H. and Mahboubi, A. (2017). The effect of salicylic acid foliar spraying on morphophysiological characteristics of common mallow and Moldavian balm. Agroecology Journal. 13(3): 25-33.

Nouri Koutanaie, F., Bernard, F., Shaker, H. and Fahimi, H. (2006). Tissue culture, Polypeptides, Gel electrophoresis, Salicylic acid, Carnation. Medical Sciences. 16 (2) :65-68.

Padash,A., Ghanbari, A. and Asgharipour, M. R. (2016). Effect of salicylic acid on Concentration of nutrients, protein and antioxidant enzymes of basil under lead stress. Iranian Journal of Plant Biology. 8(27): 17-32.

Pandey, A. C., Sanjay, S. S., and Yadav, R. S. (2010). Application of ZnO nanoparticles in influencing the growth rate of Cicer arietinum L. Journal of Experimental Nanoscience. 5 (6): 488-497.

Peyvandi, M., Kamalijamakani, Z. and Mirza, M. (2011). The effect of Iran’s iron nano-kalat with iron kalat on growth and activity of antioxidant enzymes of savory, the magazine of cellular-molecular Biotechnology news. 2(5): 25-32.

Pirasteanooshe, H., Emam, Y., Roosta, M. J. and Hashemi, S. A. (2016). The effect of salicylic acid spraying on biochemical properties and the operation of barleycorn, Iranian journal of crop science. 18(3): 232-244.

Prochazka, S., Machaackova, I., Kreekule, J. and Sebanek , J. (1998). Plant physiology Academia Praha. 484 pp.

Ramroodi, M., Keykhazhaleh, M., Galavi, M., Saghaleslami, M. J. and Baradaran, R. (2011). The effect of micronutrient ingredients and irrigation regimes on quantitative and qualitative of fleawort, Journal of Agricultural Ecology. 3(2): 219-226.

Rasouli, F., Gholipoor, M., Jahanbin, K. and Asghari, H. R. (2018). Effect of salicylic acid and jasmonic acid on induction of oxidative stress, increasing resistance and yield of Echinacea. Journal of Crop Production. 11(2): 109-122.

Rezayatmand, Z., Khavari-Nejad, R. A. and Asghari, Gh. (2013). The effect of salicylic acid on some of physiological and biochemical parameters of Artemisia aucheri Boiss. under salt stress. Journal of Plant Biology. 5(16): 57-70.

Roosta, H. R., Rzakhanim D., Raghami, M. R. and Esmaeilizadeh, M. (2017). Comparison of the effect of nano-chelate iron with fe-chelate on growth and physiological characteristics of two cultivars of Pepper in soilless culture system. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center. 8 (1): 35-55.

Rostami, Gh., Moghaddam, M., Ghasemi Pirbalouti, A. and Tehranifar, A. (2018). The effects of iron and zinc spraying in sulfate and nano forms on morphological and biochemical properties of peppermint (Mentha piperita L.) under salinity stress. Environmental Stresses in Crop Sciences. 11 (3): 707-720.

Sairam, R.K., Rao, K. V. and Srivastava, G. C. (2002). Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science. 163: 1037-1046.

Salahiostad, M., Morshadloo, M. and Moghaddam, M. (2021). Effect of silicon oxide and salicylic acid on yield, yield components, and some biochemical properties of Persian melon (Cucumis melo var. inodorus). Journal of Plant Environmental Physiology. 16(63): 72-86.

Salarpoorgharba, F. and Farahbakhsh, H. (2014).  The effects of drought tension and salicylic acid on physiological traits of fennel. Iran crop. 16(3): 765-778.

Salarpour, F. and Farahbakhsh, H. (2016). Effects of salicylic acid on some physiological traits, yield and yield components of fennel (Foeniculum vulgare Mill.) under drought stress. Iranian Journal of Medicinal and Aromatic Plants. 32(2): 216-230.

Sartip, H. and Sirousmehr, A. R. (2017). Evaluation of salicylic acid effects on growth, yield and some biochemical characteristics of cumin (Cuminum cyminum L.) under three irrigation regimes. Environmental Stresses in Crop Sciences. 10(4): 547-558.

Shafaghat, Z. and Zarinkamar, F. (2018). Tracing mucilage compounds in different stage of development of viola odorata L. leaf. Iranian Journal of Plant Biology. 31(2): 259-269.

Sharma, P. and Shanker, R. (2005). Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation. 46: 209-221.

Sheligl, H. Q. (1986). Die verwertung orgngischer souren durch chlorella lincht. Planta, 47-51.

Tabatabaie, S. G. (2009). Principles of mineral nutrition of plants. First Edition, Author Publications, 389p.

Tari, I., Csiszar, J, Szalai, G., Horvath, F., Pecsvaradi, A., Kiss, G., Szepsi, A., Szabo, M. and Erdei, L. (2002). Acclimation of tomato plants to salinity stress after a salicylic acid pre-treatment. Acta Biologica Szegediensis. 46 (3-4): 55-56.

Toosi, P., Tajbakhsh, M. and Esfehani, M. (2014). The effects of spraying iron nano-kalat on leaf growth, carbohydrate and antioxidant levels. Progress and Function of Plants. 16(2): 125-136.

Torabian, Sh. and Zahedi, M. (2013). The effect of foliaceous nutrition of iron sulfate in two types of common and nanoparticles on growth of sunflower statistics under salinity tension. Iran’s Journal of Crop Science. 44.

Vattani, H., Keshavarz, N., and Baghaei, N. (2012). Effect of sprayed Soluble different levels of iron chelate Nano fertilizer on nutrient uptake efficiency in two varieties of spinach (Varamin88 And Virofly). International Research Journal of Applied and Basic Sciences. 3(S):2651-2656.

White, R. E. (1976). Studies on the mineral ion absorption by plants, the interaction of Aluminium phosphate and pH on the growth of medicago sativa. Journal of Plant and soil 46: 195-208.

Yousefzadeh, S., Naghdibadi, H. A., Sabaghnia, N. and Janmohamadi, M. (2016). The effects of spraying iron nano-kalat physiological and chemical properties of dracocephalum moldavica. Journal of Medicinal Plants. 15(4): 152-160.

Zayed, B.A., Salem, A.K.M. and Sharkawy, H.M. (2011). Effect of different micronutrient treatments on rice (Oriza sativa L.) growth and yield under saline soil conditions. World Journal of Agricultural Sciences. 7:179- 184.