بررسی محتوی متابولیتی و فعالیت آنتی‌اکسیدانی اندام‌های مختلف علف‌هرز مهاجم نیلوفر پیچ (Ipomoea tricolor Cav.) در مزارع گنبد کاووس
محورهای موضوعی : ژنتیکابراهیم غلامعلی پور علمداری 1 , عالیه سیدی 2 , حسین صبوری 3 , زینب اورسجی 4 , عباس بیابانی 5
1 - گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.
2 - شناسایی و مبارزه با علف‌های‌هرز، دانشکده کشاورزی و منابع‌طبیعی دانشگاه گنبد کاووس، گنبدکاووس، ایران.
3 - گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.
4 - گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.
5 - گروه تولیدات گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه گنبد کاووس، گنبد کاووس، ایران.
کلید واژه: فنل کل, آنتوسیانین‌ها, اندام برگ, تجزیه اکسیداتیو, روش DPPH,
چکیده مقاله :
هدف از این آزمایش، ارزیابی محتوی متابولیتی (اولیه و ثانویه) و فعالیت آنتی‌اکسیدانی اندام‌های مختلف علف‌هرز نیلوفر پیچ نظیر ساقه، برگ و میوه به‌علاوه مخلوطی از آن‌ها بود. ابتدا علف‌هرز مورد برسی در مرحله میوه‌دهی جمع‌آوری و به‌تفکیک اندام جدا گردید. فعالیت آنتی‌اکسیدانی اندام‌های مختلف نیلوفر پیچ به روش DPPH مورد اندازه‌گیری قرار گرفت. نتایج نشان داد که مخلوطی از اندام‌های علف‌هرز نیلوفر پیچ و سپس برگ از بیشترین مقدار فنل کل برخوردار بودند. در مورد آنتوسیانین‌ها، بیشترین مقدار آن‌ها به برگ اختصاص داشت. در حالی‌که ساقه از کمترین میزان هر دوی متابولیت‌های ثانویه برخوردار بود. هم‌چنین نتایج نشان داد که بیشترین میزان قندهای محلول و پرولین به اندام برگ اختصاص داشت. ضرایب همبستگی داده‌ها نشان داد که میزان آنتوسیانین‌ها در اندام‌های مختلف نیلوفر پیچ رابطه مثبت و معنی‌داری با قندهای محلول و اسید آمینه پرولین داشتند. مطابق نتایج، با افزایش میزان متابولیت ثانویه آنتوسیانین‌ها در اندام‌های مختلف، میزان نشاسته و پروتئین به‌طور معنی‌داری کاهش یافت. مقایسه میانگین فعالیت آنتی‌اکسیدانی اندام‌های مختلف نیلوفر پیچ نشان داد که برگ و میوه به‌ترتیب از بیشترین و کمترین فعالیت آنتی‌اکسیدانی در مهار رادیکال‌های آزاد برخوردار بودند. با توجه به تاثیرگذاری نوع اندام علف هرز نیلوفر پیچ بر کمیت محتوی متابولیتی به‌ویژه ترکیبات ثانویه نظیر فنل‌ها، آنتوسیانین‌ها به همراه فعالیت آنتی‌اکسیدانی، شاید بتوان این گیاه بخصوص اندام برگ را به‌عنوان کاندیدی قابل تامل برای تجزیه اکسیداتیو رادیکال‌های آزاد، بهبود ارزش تغذیه‌ای غذا و یا علف‌کش‌ها با منشاء طبیعی با توجه به زیست توده بالا معرفی نمود.
The purpose of this study was to evaluate metabolites content (primary and secondary) and antioxidant activity of different organs of Ipomoea tricolor weed including stem, leaf, and fruit leaf, individually and in combination. The weed under study was first collected at fruit stage and divided into organs. Antioxidant activities of various organs of Ipomoea tricolor were measured according to the method of DPPH. Results showed that mixed organs of Ipomoea tricolor and then leaf had maximum total phenol contents in that order. In case of anthocyanins, the highest levels were obtained from leaf while the lowest content of both secondary metabolites were found in the stem. Results also showed that leaves had maximum soluble sugars and proline contents. Correlation coefficient of data showed a significant positive relationship between anthocyanin contents of various organs of Ipomoea tricolor and their soluble sugar and proline contents. According to the findings, content of protein and starch in various organs significantly decreased with an increase in the secondary metabolite contents of anthocyanins. Mean comparison of antioxidant activity of various organs of Ipomoea tricolor showed that leaf and fruit had the highest and lowest antioxidant activities for inhibiting free radicals, respectively. Regarding the effect of the type of Ipomoea tricolor weed organ on the quantity of metabolites especially secondary compounds like phenols and anthocyanins and also antioxidant activity, the plant, especially the leaves may be introduced as a notable candidate for oxidative decomposition of free radicals, improving the nutritional value of food, or as a natural herbicide due to its high biomass.
Afshar Mohammadian, M., Sharifi, M., Abolghasemi, S.N. and Mohammadi, N. (2015). Investigation of some medicinal secondary metabolites and antioxidants of Dittrichia graveolens L. Greuter. Nova Biologica Reperta. 2: 140-150.
AOAC. (2003). Official Methods of Analysis of AOAC International. 17 editions. 2 revisions. Gaithersburg, MD, USA, Association of Analytical Communities.
Bates, L.S., Walderen, R.D. and Taere, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.
Banerjee, D., Chakrabarti, S., Hazra, A.K., Banerjee, S., Ray, J. and Mukherjee, B. (2008). Antioxidant activity and total phenolic of some mangroves in Sundarbans. African Journal of Biotechnology. 7(6): 805-810.
Bhadoria, P.B.S. (2011). Allelopathy: a natural way towards weed management. American Journal of Experimental Agriculture. 1: 7.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft und Technologie. 28:25- 30.
Bryson, C.T. and Defelice, M.S. (2010). Weeds of the Midwestern United States and Central Canada, Athens, GA: university of Georgia press. 195p.
Bystrická, J., Vollmannová, A., Margitanová, E. and Čičová, I.. (2010). Dynamics of polyphenolics formation in different plant parts and different growth phases of selected buckwheat cultivars. Acta Agriculturae Slovenica. 95: 225-229.
Caceres, A. (2000). Calidad de la material prima para la elaboracion de productos fitofarma ceuticas. Primer Congreso International FITO 2000 Por la investigacion, conservacion y diffusion del conocimiento de las plantas medicinals 27-30 de septiembre, Lima, Peru.
Cary, D.B. and Wink, M. (1994). Elevation variation of quinlizidine alkaloid contents in a lupine (Lupinus argenteus) of the Rocky Mountains. Journal of Chememical Ecology. 20 (4): 849- 57.
Dlnoaz Hashimloyan, B., Ataiazimin1, A. and Mozhdehi, M. (2015). Identification and measurement of some secondary metabolites of leaves, stems and roots of Dendrostellera lessertii and their allelopathy effects on barley and mung bean plants. Journal of Plant Ecophysiology. 22:167-172.
Friedman, J.M., Auble, G.T., Shafroth, P.B., Scott, M.L., Merigliano, M.F., Freehling, M.D. and Griffin, E.R. (2005). Dominance of non-native riparian trees in western USA. Biological Invasions. 7(4): 747-751.
Farooq, M., Jabran, K., Cheema, Z.A., Wahid, A. and Siddique, K.H. (2011). The role of allelopathy in agricultural pest management. Pest Management Science. 67: 493-506.
Ghahreman, A. (1994). Iran Chromophytes, Volume 4. Tehran University Publication center. 618p. (In Persian)
Henríquez, C., Almonacid, S., Escobar, B., Chiffelle, I., Gómez, M. and Speisky, H. (2009). Antioxidant content and activity in different structures of five apple cultivars grown in Chile. Acta Horticulturae. 841: 275-280.
Ibrahim, M.H., Jaafar, H.Z.E., Rahmat, A. and Abdul Rahman, Z. (2011). The relationship between phenolics and flavonoids production with total non structural carbohydrate and photosynthetic rate in Labisia punila benth. Under high CO2 and nitrogen fertilization. Molecules. 16: 162 - 74.
Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J.M. (2003). Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry. 83: 547-550.
Kamali, M., Khosroyar, S. and Jalilvand, M. (2015). Evaluation of phenols, flavonoids, thocyanins and antioxidant capacity of different extracts of the aerial parts of medicinal plant Dracocephalum kotschyi. Journal of North Khorasan University of Medical Sciences. 6(3): 627-634. (In Persian)
Kochert,G.(1978). Carbohydrate determination by phenol-sulfuric acid method. In: J.A. Hellebust and J.S. Craige, Editors, Handbook of physiological and biochemical methods, Cambridge University Press, London. pp. 95–97.
Lowry, O.H., Rosebrough, N.J. and Rand, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 193: 265-273.
Malick, C.P. and Singh, M.B. (1980). In plant Enzymology and Histo Enzymologhy, Kalyani Publishers, New Dehli.
Mehrpour, M., Kashfi, B. and Moghadam, M. (2016). Study of phytochemical compounds and antoxidant of various organs from medicinal plant of Ferula assafoetida L.in two natural habitat of Semnan and Khorasan provinces. Eco-phytochemical Journal of Medicinal Plants. 4(1): 56-68. (In Persian)
Mita, S., Murano, N., Akaike, M. and Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that are inducible by sugars. Plant Journal.11: 841-851.
Olofsdotter, M. and Navarez, D. (1998). Allelopathy in rice. International rice Research Institue Manila Philippines. 154p.
Omidi, A., Rahdari, S. and Hassanpour Fard, M. (2014). A preliminary study on antioxidant activities of saffron petal extracts in lambs. Veterinary Science Development. 4(5161): 1- 4.
Rashed Mohassel, M.H., Naajafi, H., Akbarzadeh, M.D. (2001). Weed Biology and Control. Ferdowsi University publications, Mashhad, Iran. 404p. (In Persian)
Sene, M., Dove, T. and Gallet, C. (2001). Relationships between biomass, and phenolic production in grain sorghum grown under different conditions. Agronomy Journal. 93: 49-54.
Swatnon, S., Buhler, M., Forcella, D.D., Gunsolus, F. and King, R.P. (1994). Estimation of crop yield loss due to interference by multiple weed species. Weed Science Journal. 42: 103-109.
Tabatabaei Raisi, A., Khaligi, A. and Kashi, A. (2007). Antioxidant activity and chemical compositions of essential oil of aerial parts of Satureja sahendica Bornm. Pharmaceutical Sciences. 3: 1-6. (In Persian)
Thayumanavan, B., Saolasivam, N. And Ohtsobo, K. (1982). Physiochemical basis for the prefunctional uses of certain rice vrieties. Plant Foods for Human Nutrition. 34:253.
Teow, C.C., Troung, V.D., McFeeters, R.F., Thompson, R.L., Pecota, K.V. and Yencho, G.C. (2007). Antioxidant activities, phenolic and β- carotene contents of sweet potato genotypes with varying flesh colors. Food Chemistry Journal. 103: 829-838.
Vogt, T. (2010). Phenylpropanoid biosynthesis. Molecular Plant. 3: 2–20.
Zeinali, Z., Hemmati, Kh. and Mazandarani, M. (2014). Aut- ecology, ethnopharmacology, phytochemistry and antioxidant activity of Ferula gummosa Boiss. In different regions of Razavi Khorasan Province. Eco-phytochemical Journal of Medicinal Plants. 1: 11-22. (In Persian)
Zovko Koncic, M., Kremer, D. and Karlovic, K. (2010). Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat. Food and Chemical Toxicology. 48: 2176-21.
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Afshar Mohammadian, M., Sharifi, M., Abolghasemi, S.N. and Mohammadi, N. (2015). Investigation of some medicinal secondary metabolites and antioxidants of Dittrichia graveolens L. Greuter. Nova Biologica Reperta. 2: 140-150.
AOAC. (2003). Official Methods of Analysis of AOAC International. 17 editions. 2 revisions. Gaithersburg, MD, USA, Association of Analytical Communities.
Bates, L.S., Walderen, R.D. and Taere, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.
Banerjee, D., Chakrabarti, S., Hazra, A.K., Banerjee, S., Ray, J. and Mukherjee, B. (2008). Antioxidant activity and total phenolic of some mangroves in Sundarbans. African Journal of Biotechnology. 7(6): 805-810.
Bhadoria, P.B.S. (2011). Allelopathy: a natural way towards weed management. American Journal of Experimental Agriculture. 1: 7.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft und Technologie. 28:25- 30.
Bryson, C.T. and Defelice, M.S. (2010). Weeds of the Midwestern United States and Central Canada, Athens, GA: university of Georgia press. 195p.
Bystrická, J., Vollmannová, A., Margitanová, E. and Čičová, I.. (2010). Dynamics of polyphenolics formation in different plant parts and different growth phases of selected buckwheat cultivars. Acta Agriculturae Slovenica. 95: 225-229.
Caceres, A. (2000). Calidad de la material prima para la elaboracion de productos fitofarma ceuticas. Primer Congreso International FITO 2000 Por la investigacion, conservacion y diffusion del conocimiento de las plantas medicinals 27-30 de septiembre, Lima, Peru.
Cary, D.B. and Wink, M. (1994). Elevation variation of quinlizidine alkaloid contents in a lupine (Lupinus argenteus) of the Rocky Mountains. Journal of Chememical Ecology. 20 (4): 849- 57.
Dlnoaz Hashimloyan, B., Ataiazimin1, A. and Mozhdehi, M. (2015). Identification and measurement of some secondary metabolites of leaves, stems and roots of Dendrostellera lessertii and their allelopathy effects on barley and mung bean plants. Journal of Plant Ecophysiology. 22:167-172.
Friedman, J.M., Auble, G.T., Shafroth, P.B., Scott, M.L., Merigliano, M.F., Freehling, M.D. and Griffin, E.R. (2005). Dominance of non-native riparian trees in western USA. Biological Invasions. 7(4): 747-751.
Farooq, M., Jabran, K., Cheema, Z.A., Wahid, A. and Siddique, K.H. (2011). The role of allelopathy in agricultural pest management. Pest Management Science. 67: 493-506.
Ghahreman, A. (1994). Iran Chromophytes, Volume 4. Tehran University Publication center. 618p. (In Persian)
Henríquez, C., Almonacid, S., Escobar, B., Chiffelle, I., Gómez, M. and Speisky, H. (2009). Antioxidant content and activity in different structures of five apple cultivars grown in Chile. Acta Horticulturae. 841: 275-280.
Ibrahim, M.H., Jaafar, H.Z.E., Rahmat, A. and Abdul Rahman, Z. (2011). The relationship between phenolics and flavonoids production with total non structural carbohydrate and photosynthetic rate in Labisia punila benth. Under high CO2 and nitrogen fertilization. Molecules. 16: 162 - 74.
Javanmardi, J., Stushnoff, C., Locke, E. and Vivanco, J.M. (2003). Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chemistry. 83: 547-550.
Kamali, M., Khosroyar, S. and Jalilvand, M. (2015). Evaluation of phenols, flavonoids, thocyanins and antioxidant capacity of different extracts of the aerial parts of medicinal plant Dracocephalum kotschyi. Journal of North Khorasan University of Medical Sciences. 6(3): 627-634. (In Persian)
Kochert,G.(1978). Carbohydrate determination by phenol-sulfuric acid method. In: J.A. Hellebust and J.S. Craige, Editors, Handbook of physiological and biochemical methods, Cambridge University Press, London. pp. 95–97.
Lowry, O.H., Rosebrough, N.J. and Rand, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry. 193: 265-273.
Malick, C.P. and Singh, M.B. (1980). In plant Enzymology and Histo Enzymologhy, Kalyani Publishers, New Dehli.
Mehrpour, M., Kashfi, B. and Moghadam, M. (2016). Study of phytochemical compounds and antoxidant of various organs from medicinal plant of Ferula assafoetida L.in two natural habitat of Semnan and Khorasan provinces. Eco-phytochemical Journal of Medicinal Plants. 4(1): 56-68. (In Persian)
Mita, S., Murano, N., Akaike, M. and Nakamura, K. (1997). Mutants of Arabidopsis thaliana with pleiotropic effects on the expression of the gene for beta-amylase and on the accumulation of anthocyanin that are inducible by sugars. Plant Journal.11: 841-851.
Olofsdotter, M. and Navarez, D. (1998). Allelopathy in rice. International rice Research Institue Manila Philippines. 154p.
Omidi, A., Rahdari, S. and Hassanpour Fard, M. (2014). A preliminary study on antioxidant activities of saffron petal extracts in lambs. Veterinary Science Development. 4(5161): 1- 4.
Rashed Mohassel, M.H., Naajafi, H., Akbarzadeh, M.D. (2001). Weed Biology and Control. Ferdowsi University publications, Mashhad, Iran. 404p. (In Persian)
Sene, M., Dove, T. and Gallet, C. (2001). Relationships between biomass, and phenolic production in grain sorghum grown under different conditions. Agronomy Journal. 93: 49-54.
Swatnon, S., Buhler, M., Forcella, D.D., Gunsolus, F. and King, R.P. (1994). Estimation of crop yield loss due to interference by multiple weed species. Weed Science Journal. 42: 103-109.
Tabatabaei Raisi, A., Khaligi, A. and Kashi, A. (2007). Antioxidant activity and chemical compositions of essential oil of aerial parts of Satureja sahendica Bornm. Pharmaceutical Sciences. 3: 1-6. (In Persian)
Thayumanavan, B., Saolasivam, N. And Ohtsobo, K. (1982). Physiochemical basis for the prefunctional uses of certain rice vrieties. Plant Foods for Human Nutrition. 34:253.
Teow, C.C., Troung, V.D., McFeeters, R.F., Thompson, R.L., Pecota, K.V. and Yencho, G.C. (2007). Antioxidant activities, phenolic and β- carotene contents of sweet potato genotypes with varying flesh colors. Food Chemistry Journal. 103: 829-838.
Vogt, T. (2010). Phenylpropanoid biosynthesis. Molecular Plant. 3: 2–20.
Zeinali, Z., Hemmati, Kh. and Mazandarani, M. (2014). Aut- ecology, ethnopharmacology, phytochemistry and antioxidant activity of Ferula gummosa Boiss. In different regions of Razavi Khorasan Province. Eco-phytochemical Journal of Medicinal Plants. 1: 11-22. (In Persian)
Zovko Koncic, M., Kremer, D. and Karlovic, K. (2010). Evaluation of antioxidant activities and phenolic content of Berberis vulgaris L. and Berberis croatica Horvat. Food and Chemical Toxicology. 48: 2176-21.